Abstract
Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
Keywords:
SMA; IMAC; Cryogel; Protein
