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Structure and function of the selectin ligand PSGL-1


P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric mucin-like 120-kDa glycoprotein on leukocyte surfaces that binds to P- and L-selectin and promotes cell adhesion in the inflammatory response. The extreme amino terminal extracellular domain of PSGL-1 is critical for these interactions, based on site-directed mutagenesis, blocking monoclonal antibodies, and biochemical analyses. The current hypothesis is that for high affinity interactions with P-selectin, PSGL-1 must contain O-glycans with a core-2 branched motif containing the sialyl Lewis x antigen (NeuAc<FONT FACE="Symbol">a</font>2<FONT FACE="Symbol">®</font>3Galß1<FONT FACE="Symbol">®</font>4[Fuc<FONT FACE="Symbol">a</font>1<FONT FACE="Symbol">®</font>3]GlcNAcß1<FONT FACE="Symbol">®</font>R). In addition, high affinity interactions require the co-expression of tyrosine sulfate on tyrosine residues near the critical O-glycan structure. This review addresses the biochemical evidence for this hypothesis and the evidence that PSGL-1 is an important in vivo ligand for cell adhesion.

P-selectin; L-selectin; E-selectin; PSGL-1; O-glycosylation; glycoprotein; mucin; tyrosine sulfate; cell adhesion; leukocytes; neutrophils

Braz J Med Biol Res, May 1999, Volume 32(5) 519-528

Structure and function of the selectin ligand PSGL-1

R.D. Cummings

Department of Biochemistry and Molecular Biology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA



Correspondence and Footnotes Correspondence and Footnotes Correspondence and Footnotes

P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric mucin-like 120-kDa glycoprotein on leukocyte surfaces that binds to P- and L-selectin and promotes cell adhesion in the inflammatory response. The extreme amino terminal extracellular domain of PSGL-1 is critical for these interactions, based on site-directed mutagenesis, blocking monoclonal antibodies, and biochemical analyses. The current hypothesis is that for high affinity interactions with P-selectin, PSGL-1 must contain O-glycans with a core-2 branched motif containing the sialyl Lewis x antigen (NeuAca2®3Galß1®4[Fuca1®3]GlcNAcß1®R). In addition, high affinity interactions require the co-expression of tyrosine sulfate on tyrosine residues near the critical O-glycan structure. This review addresses the biochemical evidence for this hypothesis and the evidence that PSGL-1 is an important in vivo ligand for cell adhesion.


Key words: P-selectin, L-selectin, E-selectin, PSGL-1, O-glycosylation, glycoprotein, mucin, tyrosine sulfate, cell adhesion, leukocytes, neutrophils


The inflammatory response in animals is initiated by the tethering and rolling of circulating leukocytes on activated endothelium. These initial interactions involve glycoconjugate ligands on the leukocyte cell surface and carbohydrate-binding proteins, termed selectins, expressed on the surfaces of both endothelial cells and leukocytes. There are three known selectins, termed P-, E- and L-selectins, and all three are Ca2+-dependent binding proteins that contain a C-type carbohydrate recognition domain at their extreme N-terminus. P-selectin is constitutively expressed in intracellular vesicles of platelets and endothelial cells and is rapidly mobilized to the surface membrane in thrombin- or histamine-stimulated cells; E-selectin expression is transcriptionally up-regulated by inflammatory cytokines, and L-selectin is constitutively expressed on the surface membrane of all leukocytes.

Although initial studies suggested that all three selectins can recognize carbohydrates containing the sialyl Lewis x antigen (NeuAca2®3Galß1®4[Fuca1®3] GlcNAcß1®R) (sialyl Lex), recent studies have now shown that each selectin demonstrates higher affinity binding to specific macromolecular ligands expressing sialylated and fucosylated glycans. To date the best characterized cell adhesion ligand for selectins is a dimeric mucin termed the P-selectin glycoprotein ligand-1 (PSGL-1). This mucin is expressed on the surface membranes of all leukocytes, but in regard to its binding to P-selectin, it is only functional on granulocytes and subclasses of lymphocytes. Interestingly, PSGL-1 may also serve as a ligand for both E- and L-selectin. The overall structure and biology of PSGL-1 have been discussed in several articles (1-5). This review will attempt to provide an overall historical framework for understanding the more important studies on this ligand and focus on exciting new developments in regard to the structure and function of PSGL-1 and its role as a selectin ligand in the inflammatory response and atherosclerosis.

Historical studies on P-selectin ligands on neutrophils

The binding of P-selectin to neutrophils is abolished upon sialidase treatment, which suggested originally that sialic acid may be a critical determinant required for P-selectin recognition (6). The possible importance of the sialyl Lex moiety to P-selectin recognition was derived from experiments showing that 1) P-selectin binds to a mutant Chinese hamster ovary (CHO) cell line expressing the sialyl Lex moiety, but not to cells lacking the epitope (7); 2) antibodies to the sialyl Lex epitope, but not the Lex epitope, can block binding of cells to P-selectin (7,8), and 3) glycans containing the sialyl Lex moiety can inhibit P-selectin-mediated adhesion (8). However, the binding of P-selectin to neutrophils displays much higher affinity interactions than to other non-myeloid cells (7), suggesting that myeloid cells possess one or more specific ligands for P-selectin. Using 125I-P-selectin blotting and affinity chromatography on immobilized human P-selectin, a glycoprotein ligand for P-selectin was identified and purified, starting with total membrane glycoproteins extracted from human neutrophils and the human promyelocytic cell line HL60 (9). The purified ligand, now known as the PSGL-1, behaves as a disulfide-bonded ~250-kDa protein in non-reducing SDS/PAGE and ~120-kDa in reducing SDS/PAGE.

Primary structure of PSGL-1

The cDNA encoding PSGL-1 was expression cloned in COS cells co-expressing an a1,3/4-fucosyltransferase (human Fuc-TIII), which permits both sialyl Lex and sialyl Lea synthesis in these cells (10). PSGL-1 is predicted to be a protein of 412 amino acids with an 18-amino acid signal sequence and a tetrapeptide consensus cleavage site for paired basic amino acid converting enzyme in leukocytes at residues 38-41 (-R-D-R-R-) (Figure 1A). Thus, the N-terminus of the mature protein begins at residue 42. There are 16 decapeptide repeating units with the consensus sequence -A-T/M-E-A-Q-T-T-X-P/L-A/T- spanning residues 118-277 in the long form of the protein and the short form is missing the residues 132-141 (11,12). Within the extracellular domain is a single Cys residue at position 320 that precedes the predicted single transmembrane domain spanning residues 321-341 and the cytoplasmic domain of residues 342-412. The coding region for human PSGL-1 is contained entirely in exon 2 of the gene, which maps to chromosome 12q24. The cDNA for the murine PSGL-1 encodes a predicted 397-amino acid protein with recognizable homology to the human sequence (13). The murine protein contains a predicted signal sequence and propeptide identical in size to human PSGL-1; the mature murine PSGL-1 is also predicted to begin at residue 42. However, the mouse homologue has only 10 decameric repeats with the consensus sequence -E-T-S-Q/K-P-A-P-T/M-E-A- that are obviously different in sequence from the human PSGL-1. The highest homology between the human and murine PSGL-1 occurs in the transmembrane (83%) and cytoplasmic domains (76%).

Figure 1
- A, Scheme of the interactions between human P-selectin and the amino-terminal domain of human PSGL-1. The many N-glycans on PSGL-1 and P-selectin are indicated, as are the O-glycans on PSGL-1. B, The major fucose-containing O-glycans of human HL60-derived PSGL-1. Glycan 1 is a trifucosylated, monosialylated, polylactosamine core-2 O-glycan and glycan 2 is a monofucosylated, disialylated core 2 O-glycan.

Each subunit of human PSGL-1 contains 70 Ser and Thr residues in the extracellular domain that are potential sites for O-glycosylation and 3 potential sites for N-glycosylation. The murine PSGL-1 also contains numerous extracellular Ser and Thr residues and two potential sites for N-glycosylation. The murine PSGL-1 also contains a single unpaired extracellular Cys at residue 307 that precedes the predicted transmembrane domain. Interestingly, the human PSGL-1 contains three predicted tyrosine sulfation sites at residues 46, 48 and 51 that fall in the consensus sequence in which Tyr residues are flanked by acidic residues. The murine PSGL-1 contains two predicted tyrosine sulfation sites at residues 54 and 56.

Structural features of PSGL-1 required for binding to P-selectin

Glycosylation of native PSGL-1

The large size and extensive glycosylation of PSGL-1 present a daunting challenge to understanding how it is specifically recognized by P-selectin. It was anticipated at first that PSGL-1 might be a high affinity and unique ligand for P-selectin by virtue of its mucin-like nature and the presumption that the ligand contained large amounts of the sialyl Lex antigen (14), thereby enhancing its avidity for P-selectin. However, as discussed below, this prediction was not correct.

Treatment of purified PSGL-1 with sialidase abolishes its binding to P-selectin, confirming the cell studies that indicated a role for sialic acid in P-selectin recognition (9,14). Interestingly, treatment of neutrophil-derived PSGL-1 with peptide N-glycosidase F, which removes most, if not all, the N-glycans of the molecule, does not affect its recognition by P-selectin, suggesting that O-glycans, but not N-glycans, are important determinants (9). This conclusion is supported by the observation that treatment of either neutrophils or purified PSGL-1 with the O-sialoglycoprotease from Pasteurella hemolytica, an enzyme that degrades sialylated mucins, blocks all interactions with P-selectin (14). Furthermore, treatment of HL60 cells with benzyl-alpha-GalNAc, which inhibits extension of O-glycans, also reduces binding of cells to P-selectin (15). Other studies demonstrated that treatment of isolated PSGL-1 with endo-ß-galactosidase, a bacterial endoglycosidase capable of degrading type-2 polylactosamine repeats [-3Galß1®4GlcNAcß1-]n, significantly reduces binding to P-selectin (16), thus indicating that the polylactosamine repeats, presumably on O-glycans, may also be important for binding.

The O-glycans of native PSGL-1 purified from human HL60 cells were determined and the results were unexpected in many ways (17). Most of the O-glycans contained a simple core-2 structure with one or two sialic acid residues and lacked fucose. Fucose was found in only two relatively minor O-glycans, termed glycan 1 and 2, as shown in Figure 1B. Both glycan 1 and 2 contain the sialyl Lex antigen; however, glycan 1, but not glycan 2, contains a polylactosamine backbone on the core-2 structure with multiple fucosyl residues. These results demonstrated that the O-glycans of PSGL-1 generally lacked fucose and that only a few O-glycans displayed the sialyl Lex antigen, presumed to be important in P-selectin binding. The fact that glycan 2 was predicted to occur in sub-stoichiometric quantities led to the expectation that glycan 1 may be the more important O-glycan for P-selectin recognition. This possibility is discussed below.

Glycosylation of recombinant PSGL-1 required for binding to P-selectin

To explore the functional importance of core-2 O-glycans and fucose residues in PSGL-1 activity, a recombinant form of PSGL-1 was expressed in CHO cells. These cells do not express the sialyl Lex antigen and lack a1,3-fucosyltransferases required for either Lex or sialyl Lex synthesis; in addition, they lack the ß1,6-N-acetylglucosaminyltransferase (C2GnT) required for core-2 O-glycan synthesis. PSGL-1 expressed in these wild type CHO cells was not able to bind P-selectin (18). However, PSGL-1 synthesized in CHO cells co-transfected with cDNA encoding human a1,3-fucosyltransferase III (Fuc-TIII) and the human C2GnT was a high affinity ligand for P-selectin (18,19). Such results suggested that expression of both the sialyl Lex antigen and C2GnT is required for PSGL-1 binding to P-selectin.

Tyrosine sulfation and the role of the PSGL-1 N-terminus

Although glycosylation of PSGL-1 is clearly important for its binding to P-selectin, other biochemical studies of the molecule have provided several clues suggesting that sulfation was also important. The first of these clues was that PSGL-1 contained tyrosine sulfate and that removal of tyrosine sulfate by bacterial aryl sulfatases abrogated binding of the molecule to P-selectin (20). Consistent with this finding, recombinant forms of PSGL-1 in which the three tyrosine residues had been mutagenized to phenylalanine also failed to bind P-selectin (21,22). In addition, treatment of cells with sodium chlorate, an inhibitor of the ATP sulfurylase that is required for synthesis of the sulfate donor phosphoadenosine phosphosulfate (PAPS), also blocked synthesis of a functional PSGL-1 molecule (21,22).

These results suggested that the critical binding domain may reside in the extreme amino terminus of PSGL-1. Four different lines of experimentation are consistent with this possibility. 1) Moore et al. (12) developed a blocking mAb (PL1) that mapped to a peptide epitope overlapping the tyrosine sulfate consensus sites, whereas non-blocking monoclonal antibodies (e.g. PL2) mapped to epitopes outside this region (23). Other studies showed that such blocking mAb prevent neutrophil, monocyte, eosinophil and lymphocyte adhesion and rolling on to P- and L-selectin (12,24,25). 2) Pouyani and Seed (22) constructed a chimeric mucin in which the first 100 amino acids from the N-terminus of PSGL-1 were grafted onto the N-termini of CD43 and CD34, two mucins that are normally expressed on leukocytes. The chimeric proteins, but not the native forms of CD43 or CD34, expressed by COS cells co-transfected with Fuc-TVII, were bound by P-selectin. 3) A chimeric form of PSGL-1, in which the 19 amino acid segment from the extreme N-terminus of mature PSGL-1 was fused to the heavy chain CH2-CH3 region of IgG1, and the recombinant glycoprotein expressed in COS cells co-transfected with human Fuc-TIII, was bound by P-selectin (21). 4) Treatment of neutrophils with the cobra venom metalloproteinase mocarhagin removed the extreme N-terminal 10 amino acid residues from PSGL-1 and abrogated its binding to P-selectin (26). All of these experiments point to a model in which the combination of tyrosine sulfate residues and oligosaccharides on the protein are required for high affinity binding to P-selectin.

An additional approach to explore the fine structure of the PSGL-1 N-terminal domain required for P-selectin recognition is to selectively mutate amino acids in that domain and test the binding of recombinant PSGL-1 to P-selectin. Such site-directed mutagenesis to replace all three N-terminal Tyr residues with Phe abolishes binding of the recombinant PSGL-1 to P-selectin, but not E-selectin (18,21,22). A more detailed number of mutations reveal that any one of the three Tyr residues can support binding of the recombinant PSGL-1 to P-selectin, indicating that only one of the three potential tyrosine sulfate residues is necessary for binding to P-selectin (27). Furthermore, mutation of the Thr residue at position 57 to Ala in the extreme N-terminus human PSGL-1 blocks binding of the recombinant molecule to P-selectin (21,22,27). Taken together, the studies on the native and recombinant PSGL-1 support the types of models shown in Figure 2, where one or more tyrosine sulfate residues acting in conjunction with nearby Thr-attached O-glycans and with a sialyl Lex antigen on the core-2 motif are recognized by P-selectin. However, it must be stressed that this model is a mere prediction. Despite the elegant experiments described above, there is still no chemical proof that such O-linked glycans, as depicted in Figure 2, occur at Thr-57 in PSGL-1; nor is there any direct proof that there is coordinate binding of such an O-glycan and one or more tyrosine sulfates.

Figure 2
- Predicted interaction between the extreme amino terminal domain of human PSGL-1 and human P-selectin. The putative sites of interactions with tyrosine sulfate residues and the sialic acid and fucose residues of the core 2 O-glycan (glycan 2 of Figure 1) are indicated.

Affinity of PSGL-1 for P-selectin

Previous studies have established that P-selectin binds to free glycans containing sialyl Lex with relatively low affinity in the millimolar range (0.1 to 1.0 mM). However, recent studies using a BIAcore apparatus and surface plasmon resonance measurements demonstrated that neutrophil-derived PSGL-1 is a high affinity ligand for P-selectin and exhibits a Kd in the range of 0.3 µM (28). The binding is characterized by a high off-rate counterbalanced by an even higher on-rate. This high affinity between PSGL-1 and P-selectin contrasts with that observed for L-selectin binding to GlyCAM-1, where the Kd is in the range of 0.1 mM, which was also determined by surface plasmon resonance measurements (29). The binding of recombinant forms of PSGL-1 to P-selectin has also recently been measured and found to have a very high affinity. The Kd of soluble P-selectin with a soluble recombinant form of PSGL-1, which was prepared using human Fuc-TIII, was determined by measuring changes in intrinsic fluorescence upon ligand binding and found to be in the range of 3 nM (30). Interestingly, when the recombinant PSGL-1 was prepared with human Fuc-TVII, the Kd was large and in the range of 80 nM (30). Although many questions remain about the relative glycosylation of native versus recombinant PSGL-1 in such studies as those cited above, these results demonstrate that PSGL-1 is a high affinity ligand for P-selectin.

Dimerization of PSGL-1

To explore the role of dimerization of PSGL-1 on its ability to mediate cell adhesion and recognition by P-selectin, two laboratories prepared a recombinant form of the molecule in which the single extracellular Cys residue in PSGL-1 was changed to Ala (31,32). Essentially both groups found that the mutated protein behaved as a monomeric protein under reducing and non-reducing conditions and that the mutated protein displayed no affinity for P-selectin. These results were taken to indicate that PSGL-1 dimerization is essential for its high affinity binding to P-selectin. However, more recent studies in our laboratory have now provided data which question the results of these studies. Similar substitution of the single extracellular Cys with either Ala or Ser appear to have no discernible effect on binding of cells expressing the mutated protein to P-selectin (Epperson TK, Ramachandran V, Patel KD, McEver RP and Cummings RD, unpublished data). Furthermore, in the latter study it was found that the mutated PSGL-1 behaved as a non-covalent dimeric protein that was readily cross-linked in the membrane form to a dimer. In addition, a small tryptic fragment of ~9 kDa, prepared from the recombinant molecule and containing the extreme N-terminal domain of the molecule, bound quantitatively to a column of immobilized P-selectin. These results suggest that covalent dimerization of PSGL-1 is not required for its functional association with P-selectin. However, because PSGL-1 can non-covalently dimerize in the membrane, more studies will be needed in the future to define the significance of dimerization on activity of the membrane-bound ligand for binding to selectins.

Signaling functions of PSGL-1

PSGL-1 may have much more than a passive role in mediating adhesion. It may also be an important signaling molecule to neutrophils. Upon activation of polymorphonuclear leukocytes there is a redistribution of PSGL-1 resulting in a lowering of affinity of activated cells for P-selectin (33). Incubation of neutrophils with either P-selectin or the monoclonal antibody PL1 to PSGL-1 stimulates tyrosine phosphorylation of several proteins and production of IL-8 (34). This pathway of activation appears to activate GTPase Ras and the MAP kinase cascade (34). Other studies indicate that ligation of neutrophils with anti-PSGL-1 monoclonal antibodies and/or P-selectin triggers ß2-integrin-dependent and genestein-sensitive cell aggregation and tyrosine phosphorylation (35). The precise molecular mechanism(s) by which PSGL-1 is triggering these biochemical changes are presently unclear. It is intriguing to consider that dimerization of the ligand may be more important to cytoskeletal interactions and cell signaling than to cell adhesion.

Studies of P-selectin ligands in vivo

In vivo approaches to understanding structure/function relationships of glycans in selectin ligand function have recently provided exciting new insights and have partly confirmed predictions about the importance of sialyl Lex and core-2 O-glycans for PSGL-1 recognition by P-selectin. Neutrophils from null mice lacking the myeloid enzyme FucT-VII bind poorly to P-, E- or L-selectin, and neutrophil efflux in experimentally induced inflammation is dramatically reduced (36). Similarly, the binding of T lymphoblasts from Fuc-TVII-null mice to P-selectin is reduced (37). Interestingly, it has recently been shown that both human Fuc-TIV and Fuc-TVII are required to synthesize polyfucosylated polylactosamine in vitro, such as found on glycan-1 (Figure 1B) (38). Whether these enzymes act cooperatively in vivo is not known. More recent studies show that the core-2 O-glycans are also critically important for neutrophil interactions with all three selectins. Neutrophils from null mice lacking the C2GnT bind poorly to P-selectin in fluid-phase studies, although the cells still demonstrate adhesion under shear stress to P-selectin that is much greater than that seen for Fuc-TVII-null mice (39). Although the structures of O- and N-glycans on murine leukocytes are not known, these studies strongly support the observations on human PSGL-1 that core-2 O-glycans containing the sialyl Lex antigen are important in P-selectin recognition.

The specific in vivo functions of PSGL-1 have been explored using both blocking antibodies to the protein and recombinant forms. A blocking mAb to PSGL-1 (PL1) and its F(ab) fragments dramatically reduced rolling of human polymorphonuclear neutrophils and HL60 cells in venules of acutely exteriorized rat mesentery, indicating that PSGL-1 is important in vivo for rolling of myeloid cells in mesenteric venules at physiologic shear stress (40). Another approach to study PSGL-1 function in vivo during inflammation is to explore its role in ischemia/reperfusion injury models, in which blood flow is blocked, thereby stimulating P-selectin expression by endothelial cells. In a rat model of ischemia/reperfusion injury, using hepatic in vivo warm ischemia and ex vivo cold ischemia in a liver transplant experiment (41), animals were treated with 100 µg of recombinant PSGL-1 injected through the portal vein at the time of total hepatic inflow occlusion or into the isolated organ. Treatment with the soluble recombinant PSGL-1 significantly enhanced rat survival and liver function and recovery. PSGL-1 may also be important for lymphocyte recruitment to sites of inflammation in vivo, since intravenous administration of antibodies to the extreme N-terminus of mouse PSGL-1 block migration of Th1 T-lymphocytes into skin undergoing cutaneous delayed-type hypersensitivity reactions (42) and block rolling of leukocytes in venules of acutely exposed mouse cremaster muscle (43). Although these studies strongly support a role for PSGL-1 in leukocyte function in vivo, many more studies are needed to more precisely define the involvement of PSGL-1, as opposed to or in concert with other selectin ligands, in the overall response to inflammation.

Accumulation of circulating leukocytes, especially monocytes, is a recognized early event in development of atherosclerosis. Some exciting new studies are suggesting that P- and E-selectin may contribute to development of early and advanced stages of atherosclerotic lesions. Mice deficient for both P- and E-selectin (P/E-/-), combined with a deficiency in the LDL receptor (LDLR -/-) as a model system, developed fatty lesions that were smaller than those in mice with normal P- and E-selectin (LDLR-/-, P/E+/+) and the development of lesions was delayed (44). However, whether PSGL-1 is involved in the development of these lesions is not yet known.

Role of PSGL-1 as a ligand for L- and E-selectin

All three selectins can bind weakly to simple glycans containing the sialyl Lex determinant; however, as demonstrated for P-selectin and PSGL-1, such binding is relatively weak and macromolecular ligands bind with higher affinity. Although several glycoproteins are recognized by L- and E-selectin, whether these ligands serve physiologically to support selectin-mediated cell adhesion is still not clear (3). Interestingly, gathering evidence is indicating that PSGL-1 may be a physiological ligand for L-selectin (45-47) and may participate in some E-selectin-dependent adhesion. For example, neutrophil tethering to P- and E-selectin is inhibitable by blocking monoclonal antibody PL1 to PSGL-1, although the inhibition is much more efficient toward P-selectin and E-selectin (45). The results suggest that PSGL-1 is a high affinity ligand for P-selectin and perhaps a low affinity ligand for E-selectin. Furthermore, this interaction of PSGL-1 with E-selectin is not dependent on tyrosine sulfation of PSGL-1 (48). More interestingly, leukocyte tethering to L-selectin under shear stress is highly inhibitable by PL1, indicating a potential role for PSGL-1/L-selectin interactions in neutrophil-neutrophil interactions as a way of amplifying the initial leukocyte accumulation that is dependent on P-selectin (49). However, there may be other mucin-like receptors for L-selectin on leukocytes, as recently suggested by studies of Ramos et al. (50). All of these results are beginning to suggest that PSGL-1 is probably a physiological ligand for P- and L-selectin and may contribute to some E-selectin-dependent interactions.

Future directions

The past few years have seen an explosive growth in our knowledge of the structures and functions of selectins, but our understanding of selectin ligands involved in cell adhesion is still limited. PSGL-1 represents the best characterized adhesion ligand to date, but many questions still remain. Do both P- and L-selectin dually recognize tyrosine sulfate and sialyl Lex residues? Are there specific binding sites for both determinants on these selectins? If only the extreme N-terminal domain of PSGL-1 is responsible for its interactions with P-selectin (and perhaps L-selectin), what is the function of the proximal region of the mucin? What is the role of dimerization of PSGL-1? How does ligation of PSGL-1, an extended mucin, function as a signaling molecule in leukocytes? What is the function of PSGL-1 in most lymphocytes, where it is expressed in a glycoform that appears incapable of binding to P-selectin? These and many more questions are being eagerly explored in many laboratories around the world, and it is anticipated that the coming years will yield exciting new insights into the function of PSGL-1 and related mucin selectin ligands.

Address for correspondence: R.D. Cummings, Department of Biochemistry and Molecular Biology, The University of Oklahoma, Health Sciences Center, 975 N.E. 10th St., BRC 417, Oklahoma City, OK 73104, USA. Fax:: +1-405-271-3910. E-mail:

Presented at the 5th Brazilian Symposium on Extracellular Matrix - SIMEC, Angra dos Reis, RJ, Brasil, September 7-10, 1998. Research supported by a grant from the National Institutes of Health (PO1 HL 45510). Received March 2, 1999. Accepted March 9, 1999.

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  • Correspondence and Footnotes

  • Publication Dates

    • Publication in this collection
      27 Apr 1999
    • Date of issue
      May 1999


    • Accepted
      09 Mar 1999
    • Received
      02 Mar 1999
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