Epidemiological status of felid herpesvirus type-1 and feline calicivirus infections in Brazil

Situação epidemiológica das infecções pelo herpesvírus felino tipo 1 e calicivírus no Brasil

Andréia Henzel Luciane Teresinha Lovato Rudi Weiblen About the authors

Abstracts

Feline calicivirus (FCV) and felid herpesvirus type-1 (FeHV-1) are the main infectious agents of domestic and wild felines worldwide. The FCV and FeHV-1 viruses were isolated in Brazil in 1988 and 2012, respectively. Serology surveys were performed among domestic feline in the State of Rio Grande do Sul and among wild felines in central Brazilian States. Felines with acute or chronic infections may become carriers for both viruses and, viral transmission occurs mainly by ocular and nasal secretions. In addition, FCV may be transmitted by oropharyngeal secretion and fomites. The clinical signs commonly observed in cats are fever, sneezing, coughing and nasal and ocular discharge; however, oral lesions are restricted to FCV infection. A systemic syndrome showing hemorrhagic lesions, alopecia, facial edema and jaundice has been associated with FCV. Attenuated as well as inactivated vaccines against FCV and FeHV-1 were developed in the middle 1970s, and they are effective at reducing the presentation/development of the diseases, but they are not capable of eliminating the persistence of FCV and FeHV-1. This article presents a brief review of the main aspects of the FCV and FeHV-1 infections, with an emphasis in the current situation on the domestic feline population from Brazil.

FCV; FeHV-1; respiratory and oral disease; vaccination.


Calicivírus felino (feline calicivirus - FCV) e herpesvírus felino tipo - 1 (felid herpesvirus type 1 - FeHV-1) são os principais agentes envolvidos descritos mundialmente infectando felinos domésticos e selvagens. No Brasil, o FCV e o FeHV-1 foram isolados e caracterizados em 1988 e 2012, respectivamente. Estudos sorológicos em felinos domésticos foram realizados no estado do Rio Grande do Sul e em felinos selvagens em alguns estados da região central do país. Felinos com infecção aguda e/ou infecção crônica podem tornar-se portadores, para ambos os vírus, e a transmissão ocorre principalmente por secreções oculares, nasais. Além disso, o FCV pode também ser transmitido por secreções orofaringeanas e fômites. Os sinais clínicos comumente observados em felinos afetados são: febre, espirros, tosse, descarga nasal e ocular; lesões orais se restringem à infecção pelo FCV; além disso, foi descrita uma síndrome sistêmica que apresenta um quadro hemorrágico, alopecia, edema de face e icterícia. Vacinas vivas e inativadas que amenizam o quadro clínico, mas não previnem infecções persistentes pelos vírus, foram desenvolvidas nos anos 70. Este artigo apresenta uma breve revisão dos principais aspectos da infecção pelo FCV e FeHV-1, com ênfase na atual situação na população de felinos domésticos do Brasil.

FCV; FeHV-1; doenças respiratórias e orais; vacinação.


INTRODUCTION:

Respiratory infections in cats are frequent and of great importance in feline internal medicine. Feline calicivirus (FCV) and feline herpesvirus type-1 (FeHV-1) are the main agents of upper respiratory tract disease (URTD) in cats (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
; THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). Chlamydophila felis, Bordetella bronchiseptica and Mycoplasma felis are the other agents involved in this syndrome; however, they are secondary to FCV and FeHV-1 infection. These two viruses account for approximately 80 to 90% of the cases of URTD, although there is a greater involvement of FCV than FeHV-1 (GASKELL & KNOWLES, 1989GASKELL, R.; KNOWLES, J. Feline respiratory disease. In Practice: Journal of British Veterinary Association, v.11, n.1, p. 23-26, 1989.). FCV also causes oral lesions, including ulcers, stomatitis and gingivostomatitis, and may also present as a highly fatal systemic disease (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). FeHV-1 can cause ocular lesions that may progress to glaucoma and corneal ulcers, and therefore blindness (GASKEL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). Vaccination against FCV and FeHV-1 to be adopted in the 1970s. However, the isolation rates have not decreased since then furthermore; URTD remains responsible for high levels of morbidity and mortality in young cats (GASKELL & KNOWLES, 1989GASKELL, R.; KNOWLES, J. Feline respiratory disease. In Practice: Journal of British Veterinary Association, v.11, n.1, p. 23-26, 1989.). The viruses are distributed worldwide and have been isolated in Brazil in the State of Rio Grande do Sul [RS] (WEIBLEN et al., 1988WEIBLEN, R. et al. Isolation of feline calicivirus from cats in Brazil. Veterinary Record, v.122, n.4, p.94-95, 1988.; HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
b). Serological studies on domestic and wild cats have been performed in RS and in the West-Central region of Brazil (RUTHNER-BATISTA et al., 2005RUTHNER-BATISTA, H.B. et al. Neutralizing antibodies against feline herpesvirus type 1 in captive wild felids of Brazil. Journal of Zoo Wildlife Medicine, v.36, p.447-450, 2005. Available from: <http://www.ufrgs.br/labvir/artigos/artigo39.pdf>. Accessed: Jan. 22, 2014.
http://www.ufrgs.br/labvir/artigos/artig...
; JOHANN et al., 2009JOHANN, J.M. et al. Serum survey for antibodies to coronavirus, herpesvirus, calicivirus, and parvovirus in domestics cats from Rio Grande do Sul, Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.61, n.3, p.752-754, 2009. Available from: <http://www.scielo.br/pdf/abmvz/v61n3/33.pdf>. Accessed: Jan. 21, 2014. doi: 10.1590/S0102-09352009000300033.
http://www.scielo.br/pdf/abmvz/v61n3/33....
; HENZEL et al., 2013HENZEL, A. et al. Serological Survey of Feline Calicivirus and Felid herpesvirus in Rio Grande do Sul, Brazil. Acta Scientiae Veterinariae, v.41, p.1-6, 2013. Available from: <http://www.ufrgs.br/actavet/41/pub1153.pdf>. Accesed: Jan. 21, 2014.
http://www.ufrgs.br/actavet/41/pub1153.p...
). The molecular characterization of FCV isolates has also been reported (HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
a). However, the current distribution and prevalence of these viruses is largely unknown in Brazil. Therefore, the present review aims to gather data on the clinical, virological and epidemiological aspects of both viruses in RS and in several other Brazilian states with an emphasis on the importance of the presence of FCV and FeHV-1 in the non-immune population of cats.

Etiology

Feline herpesvirus type-1 (FeHV-1)

FeHV-1 is the infectious agent responsible for feline viral rhinotracheitis [FVR] (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). The initial isolation of FeHV-1 was described in 1957 in North America, and strain "C-27" was referred to as the virus prototype (CRANDELL & MAURER, 1958CRANDELL, R.A.; MAURER, F.D. Isolation of a feline virus associated with intranuclear inclusion bodies. Proceedings of the Society for Experimental Biology and Medicine, v.97, n.3, p.487-490, 1958.).

FeHV-1 belongs to the Varicellovirus genus, which is in the Alphaherpesvirinae subfamily of the Herpesviridae family. FeHV-1 consists of double-stranded DNA of 134 kilobases (Kb) with icosahedral capsids surrounded by a lipid envelope, presence of tegument between envelope and the capsid, and the diameter of virions range from 120 to 300nm (POVEY, 1979POVEY, R.C. A review of feline viral rhinotracheitis (feline herpesvirus I infection). Comparative Immunology Microbiology and Infectious Diseases, v.2, n.2-3, p.373-387, 1979. Avaiable from: <http://www.sciencedirect.com/science/article/pii/0147957179900237>. Accessed: Jan. 22, 2014. doi: 10.1016/0147-9571(79)90023-7.
http://www.sciencedirect.com/science/art...
; GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). The genome encodes five envelope glycoproteins that are important in the induction of humoral and cellular immunity (MAEDA et al., 1998MAEDA, K. et al. Properties and functions of feline herpesvirus type 1 glycoproteins. Journal of Veterinary Medical Science, v.60, n.8, p.881-888, 1998. Available from: <https://www.jstage.jst.go.jp/article/jvms/60/8/60_8_881/_pdf>. Accessed: Jan. 21, 2014. doi: 10.1292/jvms.60.881.
https://www.jstage.jst.go.jp/article/jvm...
). Some FeHV-1 isolates may differ in virulence, although all of them belong to one serotype (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

Epidemiology

FeHV-1 is distributed worldwide and endemic in most populations (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). The information available in Brazil consists of serological studies in wild and domestic cats (RUTHNER-BATISTA et al., 2005RUTHNER-BATISTA, H.B. et al. Neutralizing antibodies against feline herpesvirus type 1 in captive wild felids of Brazil. Journal of Zoo Wildlife Medicine, v.36, p.447-450, 2005. Available from: <http://www.ufrgs.br/labvir/artigos/artigo39.pdf>. Accessed: Jan. 22, 2014.
http://www.ufrgs.br/labvir/artigos/artig...
; FILONI et al., 2006FILONI, C. et al. First evidence of feline herpesvirus, calicivirus, parvovirus, and Ehrlichia exposure in Brazilian free-ranging felids. Journal of Wildlife Disease, v.42, n.2, p.470-477, 2006. Available from: <http://www.jwildlifedis.org/doi/pdf/10.7589/0090-3558-42.2.470>. Accessed: Jan. 20, 2014. doi: 10.7589/0090-3558-42.2.470.
http://www.jwildlifedis.org/doi/pdf/10.7...
; JOHANN et al., 2009JOHANN, J.M. et al. Serum survey for antibodies to coronavirus, herpesvirus, calicivirus, and parvovirus in domestics cats from Rio Grande do Sul, Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.61, n.3, p.752-754, 2009. Available from: <http://www.scielo.br/pdf/abmvz/v61n3/33.pdf>. Accessed: Jan. 21, 2014. doi: 10.1590/S0102-09352009000300033.
http://www.scielo.br/pdf/abmvz/v61n3/33....
; HENZEL et al., 2013HENZEL, A. et al. Serological Survey of Feline Calicivirus and Felid herpesvirus in Rio Grande do Sul, Brazil. Acta Scientiae Veterinariae, v.41, p.1-6, 2013. Available from: <http://www.ufrgs.br/actavet/41/pub1153.pdf>. Accesed: Jan. 21, 2014.
http://www.ufrgs.br/actavet/41/pub1153.p...
), and isolation of FeHV-1 in RS (HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
b).

Members of the Felidae family are the susceptible hosts, and there are no age, sex or breed predispositions to infection; however, the infection is more severe in cats of up to six months of age (POVEY 1979POVEY, R.C. A review of feline viral rhinotracheitis (feline herpesvirus I infection). Comparative Immunology Microbiology and Infectious Diseases, v.2, n.2-3, p.373-387, 1979. Avaiable from: <http://www.sciencedirect.com/science/article/pii/0147957179900237>. Accessed: Jan. 22, 2014. doi: 10.1016/0147-9571(79)90023-7.
http://www.sciencedirect.com/science/art...
; GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). The prevalence varies according to the population density; it may be lower than 1% in small groups and between 10 to 20% in large populations (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). Morbidity may reach 100% in young cats and in large populations, including those found in catteries, fairs and exhibitions, and mortality is high among newborns, young and debilitated cats. Recovery may occur in seven to 10 days. However, mortality may reach up to 50%, associated to generalized infection among predisposed cats (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

FeHV-1 persists in the population mainly by contact between cats with acute infection and susceptible cats. Latent infection, which is the persistence of the virus in clinically convalescent or vaccinated cats, is another important reason for the perpetuation of the virus (GASKELL & KNOWLES, 1989GASKELL, R.; KNOWLES, J. Feline respiratory disease. In Practice: Journal of British Veterinary Association, v.11, n.1, p. 23-26, 1989.). Approximately 90% of cats are seropositive all over the world. Furthermore, 80 to 100% of seropositive cats remain carriers for life, and approximately 45% may shed the virus intermittently (GASKELL & POVEY, 1979GASKELL, R. M.; POVEY, R.C. Feline viral rhinotracheitis: sites of virus replication and persistence in acutely and persistently infected cats. Research in Veterinary Science, v.27, n.2, p.167-174, 1979.). Antibodies against FeHV-1 were detected in 50-75% of the cat population in Great Britain and in 50 to 70% of the adult domestic cats in Germany (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). In Brazil, 38.1% of 97 samples showed antibodies against FeHV-1 in a serological survey conducted in Pelotas, RS (JOHANN et al., 2009JOHANN, J.M. et al. Serum survey for antibodies to coronavirus, herpesvirus, calicivirus, and parvovirus in domestics cats from Rio Grande do Sul, Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.61, n.3, p.752-754, 2009. Available from: <http://www.scielo.br/pdf/abmvz/v61n3/33.pdf>. Accessed: Jan. 21, 2014. doi: 10.1590/S0102-09352009000300033.
http://www.scielo.br/pdf/abmvz/v61n3/33....
). The prevalence of antibodies against FeHV-1 was 30.6% in another study conducted with 630 serum samples provided by veterinary hospitals of the Federal University of Santa Maria (UFSM), University of Passo Fundo (UPF) and Federal University of Rio Grande do Sul (UFRGS) and from clinics in other cities of RS (HENZEL et al., 2013HENZEL, A. et al. Serological Survey of Feline Calicivirus and Felid herpesvirus in Rio Grande do Sul, Brazil. Acta Scientiae Veterinariae, v.41, p.1-6, 2013. Available from: <http://www.ufrgs.br/actavet/41/pub1153.pdf>. Accesed: Jan. 21, 2014.
http://www.ufrgs.br/actavet/41/pub1153.p...
). Furthermore, FeHV-1 was isolated from single infections or co-infections with FCV in 26 of 302 samples from the cat population of RS. Between the 26 isolates, 14 were derived from cats with respiratory signs and 12 from asymptomatic felines (HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
b).

Serological studies suggest that FeHV-1 are also present in wild feline kept in captivity in Europe (DANIELS et. al., 1999DANIELS, M.J. et al. Feline viruses in wildcats from Scotland. Journal of Wildlife Disease, v.35, n.1, p.121-124, 1999. Available from: <www.jwildlifedis.org/doi/pdf/10.7589/00903558-35.1.121>. Accessed: Jan. 20, 2014. doi: 10.7589/0090-3558-35.1.121.
www.jwildlifedis.org/doi/pdf/10.7589/009...
) and Brazil (RUTHNER-BATISTA et al., 2005RUTHNER-BATISTA, H.B. et al. Neutralizing antibodies against feline herpesvirus type 1 in captive wild felids of Brazil. Journal of Zoo Wildlife Medicine, v.36, p.447-450, 2005. Available from: <http://www.ufrgs.br/labvir/artigos/artigo39.pdf>. Accessed: Jan. 22, 2014.
http://www.ufrgs.br/labvir/artigos/artig...
; FILONI et al., 2006FILONI, C. et al. First evidence of feline herpesvirus, calicivirus, parvovirus, and Ehrlichia exposure in Brazilian free-ranging felids. Journal of Wildlife Disease, v.42, n.2, p.470-477, 2006. Available from: <http://www.jwildlifedis.org/doi/pdf/10.7589/0090-3558-42.2.470>. Accessed: Jan. 20, 2014. doi: 10.7589/0090-3558-42.2.470.
http://www.jwildlifedis.org/doi/pdf/10.7...
). Antibodies were detected in studies conducted in Brazil in the serum from oncilla (Leopardus tigrinus), margay (Leopardus wiedii), ocelot (Leopardus pardalis), eyra cat (Herpailurus yaguarondi) jaguarundi (Herpailurus yaguarondi), cougar (Puma concolor) and jaguar (Panthera onca) (RUTHNER-BATISTA et al., 2005RUTHNER-BATISTA, H.B. et al. Neutralizing antibodies against feline herpesvirus type 1 in captive wild felids of Brazil. Journal of Zoo Wildlife Medicine, v.36, p.447-450, 2005. Available from: <http://www.ufrgs.br/labvir/artigos/artigo39.pdf>. Accessed: Jan. 22, 2014.
http://www.ufrgs.br/labvir/artigos/artig...
). In another study, 21.6% of the serum samples from cougars (Puma concolor) and leopards (Panthera pardus) were positive for FeHV-1 (FILONI et al., 2006FILONI, C. et al. First evidence of feline herpesvirus, calicivirus, parvovirus, and Ehrlichia exposure in Brazilian free-ranging felids. Journal of Wildlife Disease, v.42, n.2, p.470-477, 2006. Available from: <http://www.jwildlifedis.org/doi/pdf/10.7589/0090-3558-42.2.470>. Accessed: Jan. 20, 2014. doi: 10.7589/0090-3558-42.2.470.
http://www.jwildlifedis.org/doi/pdf/10.7...
).

Pathogeny and clinical signs

FeHV-1 infection occurs through nasal, conjunctival and oral routes. The infection is characterized by lytic replication in the epithelial cells of the mucous membranes of the nasal septum, turbinates, nasopharynx and tonsils; in addition, the conjunctiva, mandibular lymph node and upper trachea may also be involved. Viremia and/or generalized infection are not frequent, and the incubation period ranges from two to six days (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

FeHV-1 establishes latency in neural tissues (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
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) and ascends the sensory nerves via axons after primary replication, with latency established in the trigeminal ganglion [TG] (MAGGS, 2005MAGGS, D.J. Update on pathogenesis, diagnosis, and treatment of feline herpesvirus type 1. Clinical Techniques in Small Animal Practice, v.20, n.2, p.94-101, 2005. Available from: <http://www.sciencedirect.com/science/article/pii/S1096286704001094>. Accessed: Jan. 20, 2014. doi: 10.1053/j.ctsap.2004.12.013.
http://www.sciencedirect.com/science/art...
). Viral DNA was detected in the TG and non-neural tissues of cats during latency reactivation. FeHV-1 returns via axons and replicates in the primary site of infection, which is usually the turbinates (NASISSE et al., 1992NASISSE, M.P. et al. Isolation of feline herpesvirus 1 from the trigeminal ganglia of acutely and chronically infected cats. Journal of Veterinary Internal Medicine, v.6, n.2, p.102-103, 1992.; MAGGS, 2005MAGGS, D.J. Update on pathogenesis, diagnosis, and treatment of feline herpesvirus type 1. Clinical Techniques in Small Animal Practice, v.20, n.2, p.94-101, 2005. Available from: <http://www.sciencedirect.com/science/article/pii/S1096286704001094>. Accessed: Jan. 20, 2014. doi: 10.1053/j.ctsap.2004.12.013.
http://www.sciencedirect.com/science/art...
). Latently infected cats may transmit FeHV-1 to their progeny because parturition and lactation are stressors that may induce reactivation and viral shedding (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

The clinical signs observed in both natural and experimental conditions are sneezing, loss of appetite, pyrexia, depression, nasal and ocular discharge (which may be serous and/or mucopurulent depending on the severity and secondary bacterial contamination), conjunctivitis (with hyperemia and/or bruising) and, severe cases, dyspnea and cough (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). Conjunctivitis may be associated with corneal ulcers and may develop into stromal keratitis and blindness (THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). Fetal infection is reported, although abortion is rare. Abortion occurs in cats experimentally infected with FeHV-1 because of the systemic effects of the infection and not directly by the replication/pathogenesis of the virus itself (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

Immunity

The immune response against FeHV-1 is related to the recognition of viral envelope glycoproteins by the feline immune system. The induction of neutralizing antibodies occurs after infection, primarily against glycoprotein gD (MAEDA et al., 1998MAEDA, K. et al. Properties and functions of feline herpesvirus type 1 glycoproteins. Journal of Veterinary Medical Science, v.60, n.8, p.881-888, 1998. Available from: <https://www.jstage.jst.go.jp/article/jvms/60/8/60_8_881/_pdf>. Accessed: Jan. 21, 2014. doi: 10.1292/jvms.60.881.
https://www.jstage.jst.go.jp/article/jvm...
). However, the titers of antibodies induced by natural infection are often low and slowly increase, and may be absent until 40 days post-infection (THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). Furthermore, natural infection or vaccination fail to induce lasting immunity, and the induced response protects against the disease, although not re-infection (GASKELL & POVEY, 1979GASKELL, R. M.; POVEY, R.C. Feline viral rhinotracheitis: sites of virus replication and persistence in acutely and persistently infected cats. Research in Veterinary Science, v.27, n.2, p.167-174, 1979.). Other mechanisms of immunity, including the complement-mediated response, have been reported (THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). Moreover, since cellular immunity is essential for the defense against viral infections, antibodies against the glycoproteins are important for the neutralization of viral particles; nevertheless, the absence of detectable antibodies in the serum of vaccinated cats does not necessarily indicate that these animals remain susceptible to infection (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

Feline calicivirus (FCV)

FCV virions are non-enveloped, with icosahedral capsids with 27 to 40nm in diameter and a single-stranded and positive polarity RNA as genome; and it belongs to the Vesivirus genus of the Caliciviridae family (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). The genome has approximately 7.7 Kb and is organized in three open reading frames (ORFs). ORF 1 encodes non-structural proteins, ORF 2 encodes the main and largest capsid protein (viral protein - VP1) and ORF 3 encodes the smallest structural protein [VP2]. ORF 2 is divided into six regions (A to F) based on genetic variability. The regions B, D and F are conserved, whereas C and E are variable. Region E is subdivided into hypervariable regions (HVR_E; RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
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, 2009). The genes encoding immunodominant epitopes are present in E region, inducing neutralizing antibodies in the host. These genes play a key role in immune evasion during persistent infection (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
).

FCV undergoes genetic mutations during replication and responds quickly to selection pressures from the host and environment (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). This genetic diversity compromises the effectiveness of vaccines. It complicates the induction of cross-protective vaccine immunity and infection control and contributes to persistent infection (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). The emergence of highly virulent FCV isolates (virulent systemic disease associated with feline calicivirus - FCV-VSD) is another key consequence of this genetic diversity (PEDERSEN et al., 2000PEDERSEN, N.C. et al. An isolated epizootic of hemorrhagic-like fever in cats caused by a novel and highly virulent strain of feline calicivirus. Veterinary Microbiology, v.73, n.4, p.281-300, 2000. Available from: <http://www.sciencedirect.com/science/article/pii/S0378113500001838>. Accessed: Jan. 22, 2014. doi: 10.1016/S0378-1135(00)00183-8.
http://www.sciencedirect.com/science/art...
). However, all FCV isolates belong to the same serotype, despite this diversity (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
).

Epidemiology

The virus is widely distributed in the cat population (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
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), with the first isolate reported in New Zealand in 1957 (FASTIER, 1957FASTIER, L.B. A new feline virus isolated in tissue culture. American Journal of Veterinary Research, v.18, n.67, p.382-389, 1957.) and in Brazil in 1988 (WEIBLEN et al., 1988WEIBLEN, R. et al. Isolation of feline calicivirus from cats in Brazil. Veterinary Record, v.122, n.4, p.94-95, 1988.).

Members of the Felidae family are the only hosts susceptible to FCV infection (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). In the United Kingdom, serological survey in wild cats showed that FCV were present in 50% of the tested sera (DANIELS et al., 1999DANIELS, M.J. et al. Feline viruses in wildcats from Scotland. Journal of Wildlife Disease, v.35, n.1, p.121-124, 1999. Available from: <www.jwildlifedis.org/doi/pdf/10.7589/00903558-35.1.121>. Accessed: Jan. 20, 2014. doi: 10.7589/0090-3558-35.1.121.
www.jwildlifedis.org/doi/pdf/10.7589/009...
). In Brazil, the serology showed the presence of antibodies against FCV in 21.6% of 21 serum samples analyzed from 1998 to 2004 (FILONI et al., 2006FILONI, C. et al. First evidence of feline herpesvirus, calicivirus, parvovirus, and Ehrlichia exposure in Brazilian free-ranging felids. Journal of Wildlife Disease, v.42, n.2, p.470-477, 2006. Available from: <http://www.jwildlifedis.org/doi/pdf/10.7589/0090-3558-42.2.470>. Accessed: Jan. 20, 2014. doi: 10.7589/0090-3558-42.2.470.
http://www.jwildlifedis.org/doi/pdf/10.7...
) collected in: cougars, leopards and jaguars. Antibodies against FCV were also detected in 56.7% of the 97 serum samples from domestic cats tested in Pelotas/RS (JOHANN et al., 2009JOHANN, J.M. et al. Serum survey for antibodies to coronavirus, herpesvirus, calicivirus, and parvovirus in domestics cats from Rio Grande do Sul, Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.61, n.3, p.752-754, 2009. Available from: <http://www.scielo.br/pdf/abmvz/v61n3/33.pdf>. Accessed: Jan. 21, 2014. doi: 10.1590/S0102-09352009000300033.
http://www.scielo.br/pdf/abmvz/v61n3/33....
). Our research group detected antibodies against FCV in 39.2% of the 630 serum samples derived from cities neighboring the UPF, UFSM and UFRGS (HENZEL et al., 2013HENZEL, A. et al. Serological Survey of Feline Calicivirus and Felid herpesvirus in Rio Grande do Sul, Brazil. Acta Scientiae Veterinariae, v.41, p.1-6, 2013. Available from: <http://www.ufrgs.br/actavet/41/pub1153.pdf>. Accesed: Jan. 21, 2014.
http://www.ufrgs.br/actavet/41/pub1153.p...
). Furthermore, FCV isolation and characterization in FeHV-1 single or mixed infections was reported in 34 of 55 positive cats in the virus isolation conducted on 302 swab samples, and such infections were more frequent in animals without clinical signs (HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
b). However, FCV has been isolated from 18 cats without any clinical manifestation at the time of collection and 4 cats with FeHV-1 co-infection confirmed by PCR (HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
b).

FCV transmission occurs through direct and indirect contact between cats. Secretions containing the virus may infect cages as well as the cleaning and food utensils in catteries, and they may be spread by human caretakers. In addition, the secretions may persist and remain infectious for up to a month on dry surfaces at room temperature and for longer periods at lower temperatures (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
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, 2009). Experimental intravenous infections and transmission through the cat flea Ctenocephalides felis have also been reported (MENCKE et al., 2009MENCKE, N. et al. Transmission of feline calicivirus via the cat flea (Ctenocephalides felis). Parasitology Research, v.105, n.1, p.185-189, 2009. Available from: <http://link.springer.com/article/10.1007%2Fs00436-009-1381-5>. Accessed: Jan. 22, 2014. doi: 10.1007/s00436-009-1381-5.
http://link.springer.com/article/10.1007...
; RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
), although there is no evidence of the existence of reservoirs or alternative hosts for FCV infection and transmission (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Moreover, a virus related to FCV was isolated in dogs; however the transmission between cats and dogs is uncertain (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). The prevalence of FCV is directly related to the number of cats present in a cattery and is higher in large groups, such as in colonies and shelters, where it may reach between 25 and 40%. In addition, the prevalence of FCV may range from 50 to 90% in large groups; however small groups the prevalence is usually 10% (RADFORD et al., 2001RADFORD, A.D. et al. Endemic infection of a cat colony with a feline calicivirus closely related to an isolate used in live attenuated vaccines. Vaccine, v.19, n.31, p.4358-4362, 2001. Available from: <http://www.sciencedirect.com/science/article/pii/S0264410X01001918>. Accessed: Jan. 22, 2014. doi: 10.1016/S0264-410X(01)00191-8.
http://www.sciencedirect.com/science/art...
).

FCV-VSD isolates cause up to 67% mortality in clinically healthy adult cats, and the epidemiology of the FCV-VSD syndrome is still poorly understood (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). In the last decade, FCV-VSD has been reported in the United States and Europe (PEDERSEN et al., 2000PEDERSEN, N.C. et al. An isolated epizootic of hemorrhagic-like fever in cats caused by a novel and highly virulent strain of feline calicivirus. Veterinary Microbiology, v.73, n.4, p.281-300, 2000. Available from: <http://www.sciencedirect.com/science/article/pii/S0378113500001838>. Accessed: Jan. 22, 2014. doi: 10.1016/S0378-1135(00)00183-8.
http://www.sciencedirect.com/science/art...
; SCHORR-EVANS et al., 2003SCHORR-EVANS, E.M. et al. An epizootic of highly virulent feline calicivirus disease in a hospital setting in New England. Journal of Feline Medicine and Surgery, v.5, n.4, p.217-226, 2003. Available from: <http://jfm.sagepub.com/content/5/4/217.long.>. Accessed: Jan. 22, 2014. doi: 10.1016/S1098-612X(03) 00008-1.
http://jfm.sagepub.com/content/5/4/217.l...
; HURLEY et al., 2004HURLEY, K.E. et al. An outbreak of virulent systemic feline calicivirus disease. Journal of the American Veterinary Medical Association, v.224, n.2, p.241-249, 2004. Available from: <http://avmajournals.avma.org/doi/abs/10.2460/javma.2004.224.241>. Accessed: Jul. 21, 2014. doi: 10.2460/javma.2004.224.241.
http://avmajournals.avma.org/doi/abs/10....
), although it has not yet been described in Brazil.

Pathogeny and clinical signs

FCV infection is associated with acute oral and/or respiratory disease, and a transient viremia may occur three to four days after the oronasal infection. Thus, the virus may be detected in other tissues (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Transplacental infection was not confirmed, although FCV has been isolated from aborted fetuses (ELLIS, 1981ELLIS, T.M. Jaundice in a Siamese cat with in utero feline calicivirus infection. Australian Veterinary Journal, v.57, n.8, p.383-385, 1981.). Although FCV infection is primarily associated with the respiratory tract, viral replication is not restricted this tissue (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
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). Strains differ in tropism and pathogenicity, and different clinical manifestations may be observed (GASKELL & WARDLEY, 1978). Infected cats may have subclinical infections or fever, pharyngitis, chronic gingivitis/stomatitis and synovitis with increased synovial fluid, which manifests in the form of lameness. The pathogenesis of synovitis is unclear, although tissue deposition of immune complexes may play a key role in the development of this condition (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
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, 2009). Death may also occur in infected cats because of pneumonia (LOVE, 1975LOVE, D.N. Pathogenicity of a strain of feline calicivirus for domestic kittens. Australian Veterinary Journal, v.51, n.12, p.541-546, 1975.) and nervous system disease (SATO et al., 2004SATO, Y. et al. Properties of a calicivirus isolated from cats dying in an agitated state. Veterinary Record, v.155, n.25, p.800-805, 2004. Available from: <http://veterinaryrecord.bmj.com/content/155/25/800.long>. Accessed: Jan. 22, 2014.
http://veterinaryrecord.bmj.com/content/...
).

The severity of clinical signs depends on the route of exposure, presence of concurrent infection, age group, viral dose and immune status of the host, including its vaccination history (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Some cats remained carriers after acute FCV infection. FCV may be in the tonsillar epithelium, although tonsillectomy does not eliminate the carrier status (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). The presence of carrier feline is common, ranging from 15 to 91%, and these cats spread the virus throughout the environment (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Certain cats shed the virus through the oropharynx for weeks, months or even years, although the mechanisms involved in the establishment of such viral persistence are not well known (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). In addition, there is evidence that FCV has promoted the evolution of the immunodominant regions of the capsid through the selection of mutants that escape neutralization by the host immune response (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
, 2009).

The pathogenesis of the FCV-VSD syndrome is not well known (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Factors such as viral evolution and/or immunomediated and environmental components may be related to the development of this syndrome (HURLEY et al., 2004HURLEY, K.E. et al. An outbreak of virulent systemic feline calicivirus disease. Journal of the American Veterinary Medical Association, v.224, n.2, p.241-249, 2004. Available from: <http://avmajournals.avma.org/doi/abs/10.2460/javma.2004.224.241>. Accessed: Jul. 21, 2014. doi: 10.2460/javma.2004.224.241.
http://avmajournals.avma.org/doi/abs/10....
). The clinical manifestations are pyrexia, cutaneous edema, ulcerative lesions in the head and gums, jaundice resulting from hepatic necrosis and pancreatitis, disseminated vasculitis and hemorrhaging (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
).

Immunity

The immune response against FCV infection depends on host factors, viral dose and strain and feline immune status. Maternal antibodies are important for protection during the first few weeks of life, although they may affect vaccination. Data on the magnitude/length of passive immunity against FCV are scarce, although the levels of antibodies against FCV are mostly high and persist for 10 to 14 weeks (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
, 2009).

Neutralizing antibodies are detectable seven days post-infection and are key for active immunity (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
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, 2009). The titers of antibodies against FCV have been correlated with protection against viral homologous strains/isolates (POVEY & INGERSOLL, 1975POVEY, C.; INGERSOLL, J. Cross-protection among feline calicviruses. Infectious Immunology, v.11, n.5, p.877-885, 1975. Available from: <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC415151/pdf/iai00233-0001.pdf>. Accessed: Jan. 22, 2014.
http://www.ncbi.nlm.nih.gov/pmc/articles...
). However, the previously developed immunity will only partly protect against heterologous strains (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Heterologous protection depends on the virus strain involved; however, a reduction in clinical signs and even of oral viral shedding in some cases may be observed (POVEY & INGERSOLL, 1975POVEY, C.; INGERSOLL, J. Cross-protection among feline calicviruses. Infectious Immunology, v.11, n.5, p.877-885, 1975. Available from: <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC415151/pdf/iai00233-0001.pdf>. Accessed: Jan. 22, 2014.
http://www.ncbi.nlm.nih.gov/pmc/articles...
). Cats exposed to the virus may not show disease even in the absence of detectable antibodies, suggesting that other immune mechanisms might be involved. The cellular immune response has been observed in cats vaccinated against FCV (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
).

Diagnosis

The diagnosis of FCV and FeHV-1 is conducted by isolation in cellular culture (CC), immunofluorescence assay (IFA), serology and polymerase chain reaction (PCR). PCR is used to detect RNA/DNA from conjunctival, nasal or oropharyngeal swabs, blood or biopsies (THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). The sensitivity of reverse transcription-PCR (RT-PCR) depends on the primers and detected strain because of the genetic variability of FCV (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
; THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
).

Isolation in CC is less sensitive than PCR, although the viruses replicate readily in Crandell Rees feline kidney (CRFK) lines (CRANDELL et al., 1973CRANDELL, R.A. et al. Development and characterization and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In vitro, v.9, p.76-85, 1973. Accessed: Jan. 20, 2014. doi: 10.1007/bf02618435.
https://doi.org/10.1007/bf02618435....
). FCV has a rapid replication rate that may complicate the identification of FeHV-1 in concurrent infections (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
; RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). PCR is a good alternative in these cases. Positive viral isolation indicates that viruses are actively replicating, albeit insufficiently in chronic infections in the case of FCV and latent FeHV-1 infections (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
; THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). Furthermore, viral inactivation during transport resulting from the presence of antibodies and extracellular fluids may affect in vitro replication (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

Antibodies against FCV and FeHV-1 may be detected by serum neutralization (SN) or enzyme-linked immunosorbent assays (ELISA) from serum, aqueous humor and cerebrospinal fluid. SN is widely used, although some cats survive a severe infection without developing detectable antibodies (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
; THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
). The paired serology may aid in diagnosis, although the difficulty in collecting sufficient amounts of blood from cats, time required obtaining results, and low antibody titer in convalescent cats and those latently infected with FeHV-1 are disadvantages of using SN. Furthermore, the presence of antibodies may not be correlated with active infection because the detection of antibodies fails to differentiate between the vaccinated cats and naturally infected cats (MAGGS, 2005MAGGS, D.J. Update on pathogenesis, diagnosis, and treatment of feline herpesvirus type 1. Clinical Techniques in Small Animal Practice, v.20, n.2, p.94-101, 2005. Available from: <http://www.sciencedirect.com/science/article/pii/S1096286704001094>. Accessed: Jan. 20, 2014. doi: 10.1053/j.ctsap.2004.12.013.
http://www.sciencedirect.com/science/art...
).

The interpretation of serology against FCV must be performed carefully. False negatives may be detected when there are neutralizing antibodies that have no cross-reaction with the strain used in the test. Higher titers may be observed with homologous strains than with heterologous strains (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
).

The diagnosis of FCV-VSD syndrome is performed based on clinical signs, epidemiology (morbidity and mortality) and macroscopic and microscopic lesions that result from biopsies; in such cases, immunohistochemistry and IFA have been used to detect viral antigens present in the tissues. In addition, the nucleotide sequencing of VP1 coding region can also be used in the diagnosis of FCV-VSD (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
).

Control, prevention and vaccination

Prevention and control measures, including good hygiene practices and vaccination with three applications starting on the ninth week of life, annual boosters after the third application, and revaccination (one dose) in the third year (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
; THIRY et al., 2009THIRY, E. et al. Feline herpesvirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.547-555, 2009. Available from: <http://jfm.sagepub.com/content/11/7/547.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.003.
http://jfm.sagepub.com/content/11/7/547....
) are required to reduce the prevalence/incidence of infection. Environmental disinfection after outbreaks of FCV and FeHV-1 infections, especially in breeding catteries or shelters, are recommended to prevent the virus from spreading. FCV may persist in the environment for approximately one month. Effective disinfectants include sodium hypochlorite, potassium monosulfate peroxide, chlorine dioxide and commercial products (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
).

Vaccines against FCV and FeHV-1 have been commercially available since the 1970s (GASKEL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
; RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
). Live and inactivated vaccines for the parenteral administration against FCV and FeHV-1 and (in some countries) live intranasal vaccines against FeHV-1 are marketed (ORR et al., 1980ORR, C.M. et al. Interaction of an intranasal combined feline viral rhinotracheitis, feline calicivirus vaccine and the FVR carrier state. Veterinary Record, v.106, n.8, p.164-166, 1980.; LAPPIN et al., 2006LAPPIN, M.R. et al. Effects of a single dose of an intranasal feline herpesvirus 1, calicivirus, and panleukopenia vaccine on clinical signs and virus shedding after challenge with virulent feline herpesvirus 1. Journal of Feline Medicine and Surgery, v.8, n.3, p.158-163, 2006. Available from: <http://jfm.sagepub.com/content/8/3/158.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2005.12.001.
http://jfm.sagepub.com/content/8/3/158.l...
). However, vaccines do not provide full protection and clinical disease and viral shedding still may occur (LAPPIN et al., 2006LAPPIN, M.R. et al. Effects of a single dose of an intranasal feline herpesvirus 1, calicivirus, and panleukopenia vaccine on clinical signs and virus shedding after challenge with virulent feline herpesvirus 1. Journal of Feline Medicine and Surgery, v.8, n.3, p.158-163, 2006. Available from: <http://jfm.sagepub.com/content/8/3/158.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2005.12.001.
http://jfm.sagepub.com/content/8/3/158.l...
); therefore, massive vaccination should be performed, aiming to reduce the incidence of the disease.

Vaccines also fail to prevent cats from being carriers of both viruses. Furthermore, the attenuated vaccine against FeHV-1 may induce latent infection (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). Vaccine failures entail possible periodic reactivations of latency, allowing transmission of the virus to susceptible cats in the case of FeHV-1 (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
). Conversely, the challenge of vaccination against FCV is related to the genetic diversity among isolates. Although the vaccine strain is considered to be broadly reactive, the vaccines fail to provide adequate protection against all of the field isolates or against the carrier status (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
, 2009). The prevalence of FCV is greater than FeHV-1 because of this incomplete vaccine protection and high FCV infectivity.

The strain that is commonly used worldwide in vaccines the FCV-F9. FCV-255 and FCV-F7 are also used and the strains FCV-G1 and FCV-431 are used in Japan and Europe, respectively (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). However, current vaccines will not prevent the FCV-VSD syndrome because outbreaks have been reported in vaccinated cats (HURLEY, 2004HURLEY, K.E. et al. An outbreak of virulent systemic feline calicivirus disease. Journal of the American Veterinary Medical Association, v.224, n.2, p.241-249, 2004. Available from: <http://avmajournals.avma.org/doi/abs/10.2460/javma.2004.224.241>. Accessed: Jul. 21, 2014. doi: 10.2460/javma.2004.224.241.
http://avmajournals.avma.org/doi/abs/10....
). An FCV-VSD outbreak after vaccination in New Zealand caused 32% mortality of cats, and different viral strains were isolated (SCHORR-EVANS et al., 2003SCHORR-EVANS, E.M. et al. An epizootic of highly virulent feline calicivirus disease in a hospital setting in New England. Journal of Feline Medicine and Surgery, v.5, n.4, p.217-226, 2003. Available from: <http://jfm.sagepub.com/content/5/4/217.long.>. Accessed: Jan. 22, 2014. doi: 10.1016/S1098-612X(03) 00008-1.
http://jfm.sagepub.com/content/5/4/217.l...
) and defined as vaccine breakdown strains [VBS] (RADFORD et al., 2007RADFORD, A.D. et al. Feline calicivirus. Veterinary Research, v.38, n.2, p.319-335, 2007. Available from: <http://www.vetres.org/articles/vetres/abs/2007/02/v06220/v06220.html>. Accessed: Jan. 22, 2014. doi: 10.1051/vetres:2006056.
http://www.vetres.org/articles/vetres/ab...
, 2009). Vaccine failures against FCV and FeHV-1 may also be related to pre-existing carrier status, intercurrent illness and/or interference of passive immunity or sub-protective vaccine titers (GASKELL & WARDLEY, 1978).

In Brazil, limited data about the vaccination status of cats included in serological and viral isolation studies conducted in RS is available. These studies have shown that approximately 10% of the populations of domestic cats are vaccinated (JOHANN et al., 2009JOHANN, J.M. et al. Serum survey for antibodies to coronavirus, herpesvirus, calicivirus, and parvovirus in domestics cats from Rio Grande do Sul, Brazil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.61, n.3, p.752-754, 2009. Available from: <http://www.scielo.br/pdf/abmvz/v61n3/33.pdf>. Accessed: Jan. 21, 2014. doi: 10.1590/S0102-09352009000300033.
http://www.scielo.br/pdf/abmvz/v61n3/33....
; HENZEL et al., 2012HENZEL, A. et al. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil. Brazilian Journal of Microbiology, v.432, n.2, p.560-568, 2012b. Available from: <http://www.scielo.br/pdf/bjm/v43n2/17.pdf>. Accessed: Jan. 20, 2014. doi: 10.1590/S1517-83822012000200017.
http://www.scielo.br/pdf/bjm/v43n2/17.pd...
b; HENZEL et al., 2013HENZEL, A. et al. Serological Survey of Feline Calicivirus and Felid herpesvirus in Rio Grande do Sul, Brazil. Acta Scientiae Veterinariae, v.41, p.1-6, 2013. Available from: <http://www.ufrgs.br/actavet/41/pub1153.pdf>. Accesed: Jan. 21, 2014.
http://www.ufrgs.br/actavet/41/pub1153.p...
).

Treatment

Many antiviral drugs used in veterinary medicine only inhibit the replication of DNA viruses or retroviruses, and antiviral therapy for FCV infections has not been introduced in the clinical practice (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Ribavirin is one of the few antiviral agents capable of inhibiting the in vitro replication of FCV, although it is apparently toxic to cats, and its side effects have prevented its systemic application (POVEY, 1978POVEY, R.C. Effect of orally administered ribavirin on experimental feline calicivirus infection in cats. American Journal of Veterinary Research, v.39, n.8, p.1337-1341, 1978.). Feline interferon (IFN) omega inhibits in vitro replication, although field studies are not available (RADFORD et al., 2009RADFORD, A.D. et al. Feline calicivirus infection ABCD guidelines on prevention and management. Journal of Feline Medicine and Surgery, v.11, n.7, p.556-564, 2009. Available from: <http://jfm.sagepub.com/content/11/7/556.long>. Accessed: Jan. 22, 2014. doi: 10.1016/j.jfms.2009.05.004.
http://jfm.sagepub.com/content/11/7/556....
). Cats severely affected by FCV-VSD have been treated with support therapy (fluid and antibiotic therapy), as well as steroids and IFN, and clinical improvement has been reported informally (FOLEY, 2006FOLEY J. et al. Virulent systemic feline calicivirus infection: local cytokine modulation and contribution of viral mutants. Journal of Feline Medicine and Surgery, v.8, n.1, p.55-61, 2006. Available from: <http://www.jfm.sagepub.com/content/8/1/55.full.pdf>. Accessed: Jul. 21, 2014. doi: 10.1016/j.jfms.2005.08.002.
http://www.jfm.sagepub.com/content/8/1/5...
).

No antiviral has been developed specifically for FeHV-1, although many have been tested for their efficacy against the virus and safety in cats. Broad-spectrum antibiotics that achieve high penetration in the respiratory tract are applied in all cases to inhibit secondary bacterial infections. Nasal discharge may be reduced by using saline solutions and topical ointments. Mucolytic drugs, including bromhexine, may also be useful (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
; FONTENELLE et al., 2008FONTENELLE, J.P. et al. Effect of topical ophthalmic application of cidofovir on experimentally induced primary ocular feline herpesvirus-1 infection in cats. American Journal of Veterinary Research, v.69, n.2, p.289-293, 2008. Available from: <http://avmajournals.avma.org/doi/abs/10.2460/ajvr.69.2.289>. Accessed: Jul. 21, 2014. doi: 10.2460/ajvr.69.2.289.
http://avmajournals.avma.org/doi/abs/10....
; MALIK et al., 2009MALIK, R. et al. Treatment of feline herpesvirus-1 associated disease in cats with famciclovir and related drugs. Journal of Feline Medicine and Surgery, v.11, n.1, p.40-48, 2009. Avaiable from: <http://www.jfm.sagepub.com/content/11/1/40.full.pdf>. Accessed: Jul. 21, 2014. doi: 10.1016/j.jfms.2008.11.012.
http://www.jfm.sagepub.com/content/11/1/...
).

Certain drugs have been proposed for the treatment of FeHV-1 ocular infections, including bromovinyldeoxyuridine, cidofovir, famciclovir, N-(2-hydroxy propyl) methacrylamide (HPMA), penciclovir, ribavirin, valacyclovir, vidarabine, foscarnet and lactoferrin (MALIK et al., 2009MALIK, R. et al. Treatment of feline herpesvirus-1 associated disease in cats with famciclovir and related drugs. Journal of Feline Medicine and Surgery, v.11, n.1, p.40-48, 2009. Avaiable from: <http://www.jfm.sagepub.com/content/11/1/40.full.pdf>. Accessed: Jul. 21, 2014. doi: 10.1016/j.jfms.2008.11.012.
http://www.jfm.sagepub.com/content/11/1/...
). The efficacy of these drugs is not supported by the published data, although topical application of cidofovir in FeHV-1 primary ocular infection has shown promising results (FONTENELLE et al., 2008FONTENELLE, J.P. et al. Effect of topical ophthalmic application of cidofovir on experimentally induced primary ocular feline herpesvirus-1 infection in cats. American Journal of Veterinary Research, v.69, n.2, p.289-293, 2008. Available from: <http://avmajournals.avma.org/doi/abs/10.2460/ajvr.69.2.289>. Accessed: Jul. 21, 2014. doi: 10.2460/ajvr.69.2.289.
http://avmajournals.avma.org/doi/abs/10....
). In vitro studies have shown that FeHV-1 is susceptible to feline IFN or recombinant human IFN-alpha and the synergistic effect of acyclovir and recombinant human IFN-alpha (GASKELL et al., 2007GASKELL, R. et al. Feline herpesvirus. Veterinary Research, v.38, n.2, p.337-354, 2007. Avaiable from: <http://www.vetres.org/articles/vetres/pdf/2007/02/v07073.pdf>. Accessed: Jan. 20, 2014. doi: 10.1051/vetres:2006063.
http://www.vetres.org/articles/vetres/pd...
).

CONCLUSION:

FCV and FeHV-1 are key agents in feline veterinary medicine and have spread globally in this population. The isolation of FeHV-1 and FCV in healthy cats in the southern region of Brazil and great diversity in the genetic sequences of Brazilian isolates of FCV, both among the isolates and in comparison with a known vaccine strain, emphasize the difficulty in in controlling and preventing these viruses. Nevertheless, vaccination is currently the recommended practice for decreasing the clinical cases and viral shedding once treatment methods are ineffective and only provide palliative care. Furthermore, prophylactic measures to avoid contact between domestic animals and feral populations and promote good hygiene practices are relevant for controlling infection and preventing viral spread.

ACKNOWLEDGEMENTS

R. Weiblen (301339/04-0) is a recipient of Brazilian Council for Research (CNPq) scholarships.

Publication Dates

  • Publication in this collection
    June 2015

History

  • Received
    14 Aug 2014
  • Accepted
    03 Nov 2014
Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br