The current assay was carried out to evaluate the mycelial biomass production and sporulation of Cercospora piaropi in V8, ETD and BD liquid media at incubation periods of 96, 120, 144, and 168 h, under constant agitation. Additionally, the effect of dehydration periods on the sporulation of mycelial biomass at 24, 48, 72, 96 and 120 h was evaluated. The obtained inocula were evaluated according to the severity of the disease on water hyacinth (Eichhornia crassipes) plants. In accordance with the results, the ETD media yielded more mycelial growth than the BD and V8 media, especially after 144 h of agitation. However, the V8 medium induced a higher pathogen sporulation rate, when cultivated for up to 120 h. The obtained inocula produced in V8 and ETD media caused higher values of disease severity. The dehydration period of the mycelial biomass from 72 h was favorable for a higher conidial production. The dehydration period has no effect on disease severit.
