Intraspecific ecomorphological variations in Poecilia reticulata (Actinopterygii, Cyprinodontiformes): comparing populations of distinct environments

Variações ecomorfológicas intraespecíficas em Poecilia reticulata (Actinopterygii, Cyprinodontiformes): comparando populações de ambientes distintos

Fábio T. Mise Fagner de Souza João P. A. Pagotto Erivelto Goulart About the authors

ABSTRACT

Morphological variations, according to the principles of ecomorphology, can be related to different aspects of the organism way of life, such as occupation of habitats and feeding behavior. The present study sought to examine the intraspecific variation in two populations of Poecilia reticulata Peters, 1859, that occur in two types of environments, a lotic (Maringá Stream) and a lentic (Jaboti Lake). Due to a marked sexual dimorphism, males and females were analyzed separately. Thus, the proposed hypotheses were that the populations that occur in distinct environments present morphological differences. The morphological variables were obtained using morphometric measurements and the ecomorphological indexes. The data were summarized in a Principal Component Analysis (PCA). A Multivariate Analysis of Variance (Manova) was made to verify significant differences in morphology between the populations. Males and females showed similar ecomorphological patterns according to the environment they occur. In general the population from Maringá Stream had fins with major areas, and the Jaboti Lake population eyes located more dorsally. Additionally, others morphological differences such as wider mouth of the males from Maringá Stream, wider heads on Jaboti Lake females and more protractible mouths on males from Jaboti Lake suggest a set of environmental variables that can possibly influence the ecomorphological patterns of the populations, as the water current, availability of food resources and predation. In summary, the initial hypotheses could be confirmed, evidencing the occurrence of distinct ecomorphotypes in the same species according to the environment type.

KEYWORDS
Ecomorphological indexes; Poeciliidae; habitat occupation; ecomorphotypes

RESUMO

Variações morfológicas, de acordo com os princípios da ecomorfologia, podem ser relacionadas a diferentes aspectos do modo de vida dos organismos, como a ocupação de hábitats e comportamento alimentar. O presente estudo buscou analisar essas variações em nível intraespecífico em duas populações de Poecilia reticulata Peters, 1859, que ocorrem em dois tipos de ambientes, um lótico (ribeirão Maringá) e um lêntico (lago Jaboti). Por apresentar um acentuado dimorfismo sexual machos e fêmeas foram analisados separadamente. Assim, a hipótese proposta foi de que as populações, que ocorrem em ambientes distintos, possuem diferenças morfológicas. As variáveis morfológicas foram obtidas por meio de medidas morfométricas e índices ecomorfológicos. Os dados foram sumarizados em uma Análise de Componentes Principais. Também foi feita uma Análise de Variância Multivariada para verificar se existe diferença significativa na morfologia entre as populações. Machos e fêmeas mostraram padrões ecomorfológicos semelhantes de acordo com o ambiente em que ocorrem. No geral, a população do ribeirão Maringá apresentou nadadeiras com maiores áreas e a população do lago Jaboti apresentou olhos localizados em região mais dorsal. Adicionalmente, outras diferenças morfológicas como bocas mais largas nos machos do ribeirão Maringá, cabeças mais largas nas fêmeas do lago Jaboti e bocas mais protráteis nos machos do lago Jaboti, sugerem um conjunto de variáveis ambientais que possivelmente influenciam os padrões ecomorfológicos das populações, como, por exemplo, a corrente de água, disponibilidade de recursos alimentares e predação. Em síntese a hipótese inicial pode ser confirmada, evidenciando a ocorrência de ecomorfótipos distintos da mesma espécie de acordo com o tipo de ambiente.

PALAVRAS-CHAVE
Índices ecomorfológicos; Poeciliidae; ocupação de hábitats; ecomorfotipos

One of the principal concepts in ecomorphology is the relation between morphology and ecological aspects (Peres-Neto, 1999Peres-Neto, P. R. 1999. Alguns métodos e estudos em ecomorfologia de peixes de riacho. In: Caramaschi, E. P.; Mazzoni, R. & Peres-Neto, P. R. eds. Ecologia de peixes de riachos. Oecologia Brasiliensis. Rio de Janeiro, UFRJ. v.VI, p. 209-236. ). Following this premise, it is expected that part of the ecological niche of species can be predicted by a set of morphological characteristics (Watson & Balon, 1984Watson, D. J. & Balon, E. K. 1984. Ecomorphological analysis of fish taxocenes in rainforest streams of northern Borneo. Journal of Fish Biology 25:371-384.). In this context, inter and intraspecific morphological variations, that are considered the natural selection's raw material (Darwin, 1859Darwin, C. 1859. On the Origin of Species. London, John Murray. 491p.), can influence the performance in the use of resources by the species and, consequently, can affect their survival (Wainwright, 1994Wainwright, P. C. 1994. Functional morphology as a tool in ecological research. In: Wainwright, P. C. & Reilly, S. M. eds. Ecological Morphology: Integrative Organismal Biology. Chicago, University of Chicago Press, p.42-59.).

Among tropical freshwater fish, the identification of the relationship between morphology and ecological aspects, like the use of food and spatial resources, can be complicated by the high opportunism degree of species (Abelha et al., 2001Abelha, M. C. F.; Agostinho, A. A. & Goulart, E. 2001. Plasticidade trófica em peixes de água doce. Acta Scientiarum 23(2):425-434.). Despite the difficulties aforementioned, recent studies have shown that there is a significant relationship between morphological variations and ecological aspects, such as feeding and habitat occupation (e.g. Teixeira & Bennemann, 2007Teixeira, I. & Bennemann, S. T. 2007. Ecomorfologia refletindo a dieta dos peixes em um reservatório no sul do Brasil. Biota Neotropica 7(2):67-76.; Oliveira et al., 2010Oliveira, E. F.; Goulart, E. Breda, L.; Minte-Vera, C. V.; Paiva, L. R. S. & Vismara, M. R. 2010. Ecomorphological patterns of the fish assemblage in a tropical floodplain: effects of trophic, spatial and phylogenetic structures. Neotropical Ichthyology 8(3):569-586.; Pagotto et al., 2011Pagotto, J. P. A.; Goulart, E. Oliveira, E. F.& Yamamura, C. B. 2011. Trophic ecomorphology of Siluriformes (Pisces, Osteichthyes) from a tropical stream. Brazilian Joural of Biology 71(2):469-479.; Santos et al., 2011Santos, A. B. I.; Camilo, F. L.; Albieri, R. J. & Araújo, F. G. 2011. Morphological patterns of five fish species (four characiformes, one perficiform) in relation to feeding habits in a tropical reservoir in South-Eastern Brazil. Journal of Applied Ichthyology 27:1360-1364.). From these studies, the interspecific morphological variation seems to have an important role in the ecological aspects, favoring the ecological diversification of the species, providing niche differentiation and, consequently, the coexistence of the species.

On the other hand, intraspecific morphological variations of some species also seem to have a key role in their occupation and survival over time in a particular habitat type (Violle et al., 2012Violle, C.; Enquist, B. J.; McGill, B. J.; Jiang, L.; Albert, C. H.; Hulshof, C.; Jung, V. & Messier, J. 2012. The return of the variance: intraspecific variability in community ecology. Trends in Ecology and Evolution 27(4):244-252.). Considering the ecomorphological paradigm, intraspecific morphological variations can result in subtle niche differences among the individuals, favoring the adaptation of some fish species according to changes in environmental conditions. These ideas are supported by studies conducted by Mittelbach et al. (1992)Mittelbach, G. G.; Osenberg, C. W & Wainwright, P. C. 1992. Variation in resource abundance affects diet and feeding morphology in the pumpkinseed sunfish (Lepomis gibbosus). Oecologia 90:8-13., Robinson et al. (1993Robinson, B. W.; Wilson, D. S.; Margosian, A. S. & Lotito, P. T. 1993. Ecological and morphological differentiation of pumpkinseed sunfish in lakes without bluegill sunfish. Evolutionaty Ecology 7:451-464.), Langerhans et al. (2003Langerhans, R. B.; Layman, C. A.; Langerhans, A. K. & DeWitt, T. J. 2003. Habitat-associated morphological divergence in two Neotropical fish species. Biological Journal of the Linnean Society 80:689-698.), and Sampaio et al. (2013Sampaio, A. L. A.; Pagotto, J. P. A.& Goulart, E.2013. Relationships between morphology, diet and spatial distribution: testing the effects of intra and interespecific morphological variations on the patterns of resource use in two Neotropical Cichlids. Neotropical Ichthyology 11(2):351-360.).

Poecilia reticulata Peters, 1859 is a widespread fish species that occurs in different kinds of habitats. The high reproduction rate (Gomiero & Braga, 2007Gomiero, L. M. & Braga, F. M. S. 2007. Reproduction of a fish assemblage in the state of São Paulo, southeastern Brazil. Brazilian Journal of Biology 67(2):283-292.) allied to its generalist behavior, pollution tolerance (Rocha et al., 2009Rocha, F. C.; Casatti, L. & Pereira, D. C. 2009. Structure and feeding of a stream fish assemblage in Southeastern Brazil: evidence of low seasonal influences. Acta Limnologica Brasiliensia 21(1):123-134.), and phenotypic plasticity (Burns et al., 2009Burns, J. G.; Nardo, P. Di & Rodd, F. H. 2009. The role of predation in variation in body shape in guppies Poecilia reticulata: a comparison of field and common garden phenotypes. Journal of Fish Biology 75:1144-1157.; Torres-Dowdall et al., 2012) contributed to its wide distribution (Alves et al., 2000Alves, D. R.; Luque, J. L.; Paraguassú, A. R. & Marques, F. A. 2000. Ocorrência de Camallanus cotti (Nematoda: Camallanidae) parasitando o Guppy, Poecilia reticulata (Osteichthyes: Poeciliidae) no Brasil. Revista Universidade Rural, Série Ciências da Vida 22:77-79.), mainly in systems where it is considered a non-native species. Therefore, investigations concerning the intraspecific morphological variation are important to identify the adaptations of this species in distinct environmental conditions.

The present study aimed to identify and analyze the intraspecific morphological variations in two populations of P. reticulata from different environments. Then, the formulated hypothesis was that the two analyzed fish populations' show different ecomorphological structure, and the morphological characteristics presented by each population can be related to habitat use and consequently to the environmental type they occur.

MATERIAL AND METHODS

Samplings were carried out in Maringá Stream (51°58'8.00"W, 23°22'28.09"S) and Jaboti Lake (23°34'00"S, 51°28'26"W). Both systems are located near urban areas in state of Paraná, Brazil, belonging respectively to the Pirapó and Ivaí River basin (Fig. 1).

Fig. 1.
Location of the sampling sites: Jaboti Lake (A) and Maringá Stream (B), state of Paraná, Brazil.

The Maringá Stream (located in Maringá municipality) is a third order stream. In the sampling site the margins were covered by grasses, and the bottom was composed of gravel and sand (Fig. 2). The Jaboti Lake (located in Apucarana city) is an artificial reservoir used for recreation, presenting lower current speed and muddy bottom (Fig. 3). The locations were chosen due to the occurrence of P. reticulata and the partial separation between the water bodies provided by a water parting. Thus, the populations can be considered partially isolated and under different environmental conditions.

Fig. 2.
Sampling site in Maringá Stream, municipality of Maringá, state of Paraná, Brazil.

Fig. 3.
Sampling site in Jaboti Lake, Apucarana city, state of Paraná, Brazil.

Samplings were conducted in April 2010 in Jaboti Lake and May 2011 in Maringá Stream. Different methods were used in order to optimize the capture of fish in the distinct environments. Electrofishing was used in the Maringá Stream, the lotic habitat (Ac current portable generator 1KW, 200v, 3-4A), throughout a 50 m stretch with nets blocking the areas downstream and upstream. On the other hand, in the Jaboti Lake, the lentic habitat, the fish capture was made in the littoral zone with a trail-net (0.5 mm between opposing knots, 15 m length and 1.20 m height).

Collected specimens were identified according to Graça & Pavanelli (2007Graça, W. J. & Pavanelli, C. S. 2007. Peixes da planície de inundação do Alto rio Paraná e áreas adjacentes. Maringá, Eduem. 241p.), separated in males and females by the analysis of external anatomy (Lucinda, 2003Lucinda, P. H. F. 2003. Family Poeciliidae (Livebearers). In: Reis, R. E.; Kullander, S. O. & Ferraris Jr., C. J. eds. Check list of the freshwater fishes of South and Central America. Porto Alegre, EDIPUCRS. p.555-581.; Graça & Pavanelli, 2007Graça, W. J. & Pavanelli, C. S. 2007. Peixes da planície de inundação do Alto rio Paraná e áreas adjacentes. Maringá, Eduem. 241p.), weighed and fixed with 10% formalin and then preserved in 70° GL ethanol. Voucher specimens (NUP13211 - Maringá Stream and NUP10010 - Jaboti Lake) were deposited in Coleção Ictiológica do Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupelia), from Universidade Estadual de Maringá (UEM) (http://peixe.nupelia.uem.br/).

A total of 78 individuals were measured, 42 from Maringá Stream (15 males and 27 females) and 36 from Jaboti Lake (13 males and 23 females). The areas and linear measures of the body were taken: standard length, body's height and width, midline body height, caudal peduncle's length, height and width, head's length, height and width, eye's height, areas of the eye and fins (dorsal, caudal, anal, pelvic and pectoral). Detailed information about the morphological measurements is described in Gatz (1979Gatz, J. R. A. J. 1979. Ecological morphology of freshwater stream fishes. Tulane Studies in Zoology and Botany 21(2):91-124.) and Oliveira et al. (2010Oliveira, E. F.; Goulart, E. Breda, L.; Minte-Vera, C. V.; Paiva, L. R. S. & Vismara, M. R. 2010. Ecomorphological patterns of the fish assemblage in a tropical floodplain: effects of trophic, spatial and phylogenetic structures. Neotropical Ichthyology 8(3):569-586.). Only adults were measured to avoid morphologic variations related to ontogeny. The linear measures were obtained using a digital caliper, and the areas from drawings of the structures previously digitalized. The software AutoCad(r) 2009 was used to calculate the areas.

For each collected species, we calculated the mean value of each morphological measurement, and subsequently calculated the ecomorphological indices. This procedure allows evaluating information restricted to differences between shapes and promotes the independence of analysis as to the size of specimens (Gatz, 1979Gatz, J. R. A. J. 1979. Ecological morphology of freshwater stream fishes. Tulane Studies in Zoology and Botany 21(2):91-124.; Winemiller, 1991Winemiller, K. O.1991. Ecomorphological diversification in lowland freshwater fish assemblages from five biotic regions. Ecological Monographs 61(4):343-365.). Although the body size is admittedly an important factor in the ecological relationships between fish, significant differences in body size may conduct the results to a trend in a variation related exclusively to specimens' size. Thus, the index use nullifies the chance of analyzes being dominated by a single variable (Winemiller, 1991Winemiller, K. O.1991. Ecomorphological diversification in lowland freshwater fish assemblages from five biotic regions. Ecological Monographs 61(4):343-365.). Despite the controversy about this procedure in ecomorphological studies (Peres-Neto, 1999Peres-Neto, P. R. 1999. Alguns métodos e estudos em ecomorfologia de peixes de riacho. In: Caramaschi, E. P.; Mazzoni, R. & Peres-Neto, P. R. eds. Ecologia de peixes de riachos. Oecologia Brasiliensis. Rio de Janeiro, UFRJ. v.VI, p. 209-236. ), some authors have used index in their analysis (Gatz, 1979Gatz, J. R. A. J. 1979. Ecological morphology of freshwater stream fishes. Tulane Studies in Zoology and Botany 21(2):91-124.; Watson & Balon, 1984Watson, D. J. & Balon, E. K. 1984. Ecomorphological analysis of fish taxocenes in rainforest streams of northern Borneo. Journal of Fish Biology 25:371-384.; Winemiller, 1991; Willis et al., 2005Willis, S. C.; Winemiller, K. O. & Lopez-Fernandez, H. 2005. Habitat structural complexity and morphological diversity of fish assemblages in a Neotropical floodplain river. Oecologia 142:284-295.; Casatti & Castro, 2006Casatti, L. & Castro, R. M. C. 2006. Testing eomorphological hypothesis in a headwater rifles fish assemblage of the rio São Francisco, southeastern Brazil. Neotropical Ichthyology 4(2):203-214.; Oliveira et al., 2010Oliveira, E. F.; Goulart, E. Breda, L.; Minte-Vera, C. V.; Paiva, L. R. S. & Vismara, M. R. 2010. Ecomorphological patterns of the fish assemblage in a tropical floodplain: effects of trophic, spatial and phylogenetic structures. Neotropical Ichthyology 8(3):569-586.). These authors assume that, by expressing the shape of morphological structures, the indices are able to reveal their ecological roles. Therefore, aware of the limitations of this approach, but at the same time, considering it an important alternative in the attempt to express the ecology of the species, in the present study ecomorphological indexes were calculated combining the morphological measures and areas: compression index; depression index; relative length, height and width of the caudal peduncle; relative length, height and width of the head; vertical eye position, relative area of the eye and fins (dorsal, anal, caudal, pectoral and pelvic). The indexes were selected according to Gatz (1979)Gatz, J. R. A. J. 1979. Ecological morphology of freshwater stream fishes. Tulane Studies in Zoology and Botany 21(2):91-124., Winemiller (1991)Winemiller, K. O.1991. Ecomorphological diversification in lowland freshwater fish assemblages from five biotic regions. Ecological Monographs 61(4):343-365., Mazzoni et al. (2010Mazzoni, R.; Moraes, M.; Rezende, C. F. & Miranda, J. C. 2010. Alimentação e padrões ecomorfológicos das espécies de peixes de riacho do alto Tocantins, Goiás, Brasil. Iheringia, Série Zoologia 100(2):162-168.), Oliveira et al. (2010)Oliveira, E. F.; Goulart, E. Breda, L.; Minte-Vera, C. V.; Paiva, L. R. S. & Vismara, M. R. 2010. Ecomorphological patterns of the fish assemblage in a tropical floodplain: effects of trophic, spatial and phylogenetic structures. Neotropical Ichthyology 8(3):569-586., and Pagotto et al. (2011Pagotto, J. P. A.; Goulart, E. Oliveira, E. F.& Yamamura, C. B. 2011. Trophic ecomorphology of Siluriformes (Pisces, Osteichthyes) from a tropical stream. Brazilian Joural of Biology 71(2):469-479.).

All ecomorphological indexes were standardized according to X’ik=(Xik - X ̅k)/SD, where Xik is the value of the index k to de specimen i, X ̅k is the mean value of the index k of the specie and SD is the standard deviation of the mean. In order to summarize the data from the ecomorphological indexes and identify trends in the morphology, a Principal Component Analysis (PCA) was performed using a correlation matrix. The Broken-Stick criterion was adopted to select the significant axis for interpretation (Jackson, 1993Jackson, D. A. 1993. Stopping rules in principal components analysis: a comparison of heuristical and statistical approaches. Ecology 74(8):2204-2214.). In complement to the PCA, a Multivariate Analysis of Variance (Manova) was performed to test significant differences of the ecomorphological indices between populations. As an alternative to control the influence of the morphological characteristics related to the sexual dimorphism in the analysis, males and females were analyzed separately, since males and females presents distinct morphology (Lucinda, 2003Lucinda, P. H. F. 2003. Family Poeciliidae (Livebearers). In: Reis, R. E.; Kullander, S. O. & Ferraris Jr., C. J. eds. Check list of the freshwater fishes of South and Central America. Porto Alegre, EDIPUCRS. p.555-581.).

All ecomorphological indexes were standardized according to is the mean value of the the specie and SD is the standard deviation of the mean. In order to summarize the data from the ecomorphological indexes and identify trends in the morphology, a Principal Component Analysis (PCA) was performed using a correlation matrix. The Broken-Stick criterion was adopted to select the significant axis for interpretation (Jackson, 1993Jackson, D. A. 1993. Stopping rules in principal components analysis: a comparison of heuristical and statistical approaches. Ecology 74(8):2204-2214.). In complement to the PCA, a Multivariate Analysis of Variance (Manova) was performed to test significant differences of the ecomorphological indices between populations. As an alternative to control the influence of the morphological characteristics related to the sexual dimorphism in the analysis, males and females were analyzed separately, since males and females presents distinct morphology (Lucinda, 2003Lucinda, P. H. F. 2003. Family Poeciliidae (Livebearers). In: Reis, R. E.; Kullander, S. O. & Ferraris Jr., C. J. eds. Check list of the freshwater fishes of South and Central America. Porto Alegre, EDIPUCRS. p.555-581.).

The PCA was performed on PcOrd(r) 5.0 software (McCune & Mefford, 1999McCune, B. & Mefford M. J. 1999. PC-ORD: multivariate analysis of ecological data. Version 4.01. Oregon, MjM Software Design.), and the Manova was conducted on R software (R Core Team, 2013R Core Team. 2013. R: A language and environment for statistical computing. Vienna, R Foundation for Statistical Computing. Available at: <Available at: http://www.R-project.org/ >. Accessed in 08/05/2015.
http://www.R-project.org/...
), using the stats package.

RESULTS

The PCAs results are summarized on Table I. According to the Broken-stick criterion only one axis was retained for interpretation for males, and two for the females. The tendencies of morphological differentiation between the populations on the first axis were similar, with the Maringá Stream population presenting negative scores and the Jaboti Lake population positive scores (Fig. 4). Also, in both ordinations the morphological indices that separated the populations were significant according to the Manova (Tab. I). Then, individuals that occur on the lotic environment can be characterized by having fins and eyes with major areas, and the individuals from the lotic environment can be characterized by having eyes positioned more dorsally.

Fig. 4.
PCA's ordination scatterplot for males and females, showing the ecomorphological indexes correlated with each axis.

For males, the Maringá Stream population showed wider mouths, and the Jaboti Lake had mouths with grater protrusion capacity. On the other hand, for the females, considering the first axis, the Jaboti Lake population was differentiated by having wider heads. On the second axis only one individual was separated in the Jaboti Lake population with major values of compression index and wider caudal peduncles, but according to the Manova only significant differences were evidenced for the relative width of the caudal peduncle.

DISCUSSION

The two analyzed populations showed distinct ecomorphological structure regardless the morphological differences related to sexual dimorphism, since males and females presented similar morphological characteristics according to the environmental pressures. The fins areas, with major values on the Maringá Stream population, are important morphological features related to swimming ability, and its role in the performance of movement on aquatic systems are well documented on literature (Gosline, 1971Gosline, W. A. 1971. Functional Morphology and Classification of Teleostean Fishes. Honolulu, University Press of Hawaii. 208p.; Gatz, 1979Gatz, J. R. A. J. 1979. Ecological morphology of freshwater stream fishes. Tulane Studies in Zoology and Botany 21(2):91-124.; Watson & Balon, 1984Watson, D. J. & Balon, E. K. 1984. Ecomorphological analysis of fish taxocenes in rainforest streams of northern Borneo. Journal of Fish Biology 25:371-384.; Kerfoot & Schaefer, 2006Kerfoot Jr., J. R. & Schaefer, J. F. 2006. Ecomorphology and habitat utilization of Cottus species. Environmental Biology of Fishes 76(1):1-13.).

On intraspecific level, Kerfoot & Schaefer (2006) Kerfoot Jr., J. R. & Schaefer, J. F. 2006. Ecomorphology and habitat utilization of Cottus species. Environmental Biology of Fishes 76(1):1-13.verified that in Cottus specimen individuals that occur on different environmental pressures also presents different morphological characteristics. Coupling field and laboratory studies, these authors found that longer fins and larger eyes occurs on individuals on high water current environment, and that these morphological features (mainly larger fins) optimize the performance in this kind of habitat. Then, the morphological tendencies with fins with greater areas found on the studied P. reticulata population from Maringá Stream corroborate with the results aforementioned.

Males from Maringá Stream presented wider mouths, and males from Jaboti Lake presented more protractible mouths (greater values of protrusion index). Both characteristics are related to feeding behavior and foraging performance (Watson & Balon, 1984Watson, D. J. & Balon, E. K. 1984. Ecomorphological analysis of fish taxocenes in rainforest streams of northern Borneo. Journal of Fish Biology 25:371-384.; Wikramanayake, 1990Wikramanayake, E. D. 1990. Ecomorphology and biogeography of a tropical stream fish assemblage: evolution of assemblage structure. Ecology 71(5):1756-1764.). In addition, females of Jaboti Lake were characterized by having wider heads, which are directly related to the size of the food consumed (Winemiller, 1991Winemiller, K. O.1991. Ecomorphological diversification in lowland freshwater fish assemblages from five biotic regions. Ecological Monographs 61(4):343-365.; Oliveira et al., 2010Oliveira, E. F.; Goulart, E. Breda, L.; Minte-Vera, C. V.; Paiva, L. R. S. & Vismara, M. R. 2010. Ecomorphological patterns of the fish assemblage in a tropical floodplain: effects of trophic, spatial and phylogenetic structures. Neotropical Ichthyology 8(3):569-586.). These morphological characteristics suggest that other factors in addition to water current can influence the ecomorphological structure of the populations, such as the food resource availability.

Studies concerning the trophic ecology of P. reticulata observed a wide range in food resources consumed by this species, occurring in some cases considerable consumption of detritus (Rocha et al., 2009Rocha, F. C.; Casatti, L. & Pereira, D. C. 2009. Structure and feeding of a stream fish assemblage in Southeastern Brazil: evidence of low seasonal influences. Acta Limnologica Brasiliensia 21(1):123-134.). Unpublished data performing stomach analysis content of P. reticulata from Maringá Stream verified great consumption of detritus and Diptera larvae (mainly from Chironomidae family). The presence of detritus in the diet of P. reticulata from streams may be related to anthropic interferences, as observed by Oliveira & Bennemann (2005Oliveira, D. C. & Bennemann, S. T. 2005. Ictiofauna, recursos alimentares e relações com as interferências antrópicas em um riacho urbano no sul do Brasil. Biota Neotropica 5(1):95-107.). The habitat homogenization caused by the removal of the riparian vegetation and other human activities can cause negative influences on the contribution of allochthonous resources, such as terrestrial insects. In homogeneous habitats, the food resources available for fish are mostly autochthonous, mainly algae, detritus and aquatic insects (Zeni & Casatti, 2014Zeni, J. O. & Casatti, L.2014. The influence of habitat homogenization on the trophic structure of fish fauna in tropical streams. Hydrobiologia 726:259-270.).

The presence of predators is other factor that may cause variation in body shape in P. reticulata (Langerhans & DeWitt, 2004Langerhans, R. B. & DeWitt, T. J. 2004. Shared and unique features of evolutionary diversification. The American Naturalist 164(3):335-349.; Torres-Dowdall et al., 2012Torres-Dowdall, J.; Handelsman, C. A.; Reznick, D. N. & Ghalambor, C. K. 2012. Local adaption and the evolution of phenotypic plasticity in Trinidadian guppies (Poecilia reticulata). Evolution 66(11):3432-3443.). Morphological characteristics in P. reticulata populations under predation pressure, according to Langerhans & DeWitt (2004)Langerhans, R. B. & DeWitt, T. J. 2004. Shared and unique features of evolutionary diversification. The American Naturalist 164(3):335-349., are more elongated bodies, larger caudal peduncle and lower position of the eye. However, predation pressure was low in Maringá Stream, with only one predator species [ Rhamdia quelen (Quoy & Gaimard, 1824)]. In Jaboti Lake, P. reticulata seems to suffer predation from two species, Hoplias malabaricus (Bloch, 1794) and Geophagus brasiliensis (Quoy & Gaimard, 1824) (Souza et al., 2015Souza, F.; Oliveira, E. F. Pagotto, J. P. A.& Silva, C. E. 2015. Estrutura ictiofaunística na zona litorânea de um lago urbano na bacia do rio Ivaí. Revista em Agronegócios e Meio Ambiente 8(1):95-110.). However, the morphological characteristics of the studied populations do not match the ones mentioned in the literature.

The differences on ecomorphological characteristics between the populations suggest a set of environmental variables influencing the fish in each studied habitat, such as water current, availability of food resources and predation. Then the initial hypothesis could be corroborated. It is important to emphasize that the variables mentioned above can act simultaneously. In this way the correlation between the morphology and ecological aspects can only be verified through experimental studies focused only in one variable. Even though, an integrative analysis including data of trophic ecology, swimming performance and observations concerning habitat occupation are important to comprehend the responses of the populations in natural conditions where the environmental variables interact.

Acknowledgments

The authors are grateful to Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais - PEA from Universidade Estadual de Maringá - UEM and to Programa de Pós-Graduação em Biologia Comparada from Universidade de São Paulo - USP for logistic and scientific support. They also thank the staff of Núcleo de Pesquisas em Limnologia Ictiologia e Aquicultura - Nupelia for the help in the fieldworks. Dra. Carla S. Pavanelli for allowing the use of the structure of the Nupélia's Ichthyological collection. The biologists and taxonomists M.Sc. Rafaela P. Ota and M.Sc. Luiz F. C. Tencatt for identifying the collected specimens. Dr. Fabrício H. Oda for revising the manuscript. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing grants to FTM, FS, JPAP.

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Publication Dates

  • Publication in this collection
    30 June 2015

History

  • Received
    24 Oct 2014
  • Accepted
    26 June 2015
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