menu menu_open
Brazil
home Table of contents navigate_before previous current next navigate_next
home Table of contents
Patologia • J. Bras. Patol. Med. Lab. 42 (3) • June 2006 • https://doi.org/10.1590/S1676-24442006000300010 linkcopy

Open-access In situ hybridization with non-radioactive riboprobes: principles and applications in pathology

AuthorshipSCIMAGO INSTITUTIONS RANKINGS

    In situ hybridization (ISH) has been used to identify mRNA (or DNA) in fresh tissue samples of humans and animals. Several protocols describing this technique are available, although its description is not usually detailed enough. The present work describes in situ hybridization reaction on fresh tissue in a way to make understandable this important technique, which allows verifying the cellular localization, and the spatial and temporal expression, of gene transcripts (mRNA). Results with PITX1, TGIF, SHH and WNT-5A riboprobes, in fresh tissue samples, are presented.

    In situ hybridization; Messenger RNA; Pathology

    vertical_align_top file_download show_chart
    location_on
    Sociedade Brasileira de Patologia Clínica, Rua Dois de Dezembro,78/909 - Catete, CEP: 22220-040v - Rio de Janeiro - RJ, Tel.: +55 21 - 3077-1400 / 3077-1408, Fax.: +55 21 - 2205-3386 - Rio de Janeiro - RJ - Brazil
    E-mail: jbpml@sbpc.org.br
    rss_feed Stay informed of issues for this journal through your RSS reader
    Accessibility / Report Error