Abstract
Passage of malaria infected blood through a two-layered column composed of acid-washed glass beads and CF 11 cellulose removes white cells from parasitized blood. However, because use of glass beads and CF 11 cellulose requires filtration of infected blood separately through these two resins and the addition of ADP, the procedure is time-consuming and may be inapropriate for use in the field, especially when large numbers of blood samples are to be treated. Our modification of this process yields parasitized cells free of contaminating leukocytes, and because of its operational simplicity, large numbers of blood samples can be processed. Our procedure also compares well with those using expensive commercial Sepacell resins in its ability to separate leukocytes from whole blood. As a test of usefulness in molecular biologic investigations, the parasites obtained from the blood of malaria-infected patients using the modified procedure yield genomic DNA whose single copy gene, the circumsporozite gene, efficiently amplifies by polymerase chain reaction.
glass beads; CF l l cellulose; leukocytes; malaria-infected human blood
Use of glass beads and CF 11 cellulose for removal of leukocytes from malaria-infected human blood in field settings
Ira F. Goldman1
Shoukat H. Qari1
Jimmie Skinner1
Salma Oliveira2
José M. Nascimento2
Marinete M. Póvoa2
William E. Collins1
Altaf A. Lal1
Atlanta. Public Health Service, CDC-NCID, Division of Parasitic Diseases. Malaria Branch, Atlanta, USA
Fundação Nacional de Saúde, Instituto Evandro Chagas, Belém, Brasil
Passage of malaria infected blood through a two-layered column composed of acid-washed glass beads and CF 11 cellulose removes white cells from parasitized blood. However, because use of glass beads and CF 11 cellulose requires filtration of infected blood separately through these two resins and the addition of ADP, the procedure is time-consuming and may be inapropriate for use in the field, especially when large numbers of blood samples are to be treated. Our modification of this process yields parasitized cells free of contaminating leukocytes, and because of its operational simplicity, large numbers of blood samples can be processed. Our procedure also compares well with those using expensive commercial Sepacell resins in its ability to separate leukocytes from whole blood. As a test of usefulness in molecular biologic investigations, the parasites obtained from the blood of malaria-infected patients using the modified procedure yield genomic DNA whose single copy gene, the circumsporozite gene, efficiently amplifies by polymerase chain reaction.
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
Dec 1992