Association between Volatile Sulfur Compounds <i>Prevotella intermedia</i> and Matrix Metalloproteinase-8 Expression

Maitimu, Fergy Christin; Soeroso, Yuniarti; Sunarto, Hari; Bachtiar, Boy Muchlis

doi:10.1590/pboci.2022.049

Fergy Christin Maitimu

Conceptualization

Methodology

Formal Analysis

Investigation

Data Curation

Writing - Original Draft

Writing - Review and Editing

contribution to critical review of intellectual content

final version approval

Residency Program, Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

http://orcid.org/0000-0001-9047-4741

Yuniarti Soeroso Correspondence: Yuniarti Soeroso, Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jl. Salemba Raya no.4, Jakarta 10430, Indonesia. E-mail: yuniarti_22@yahoo.co.id

Conceptualization

Methodology

Formal Analysis

Investigation

Data Curation

Writing - Original Draft

Writing - Review and Editing

contribution to critical review of intellectual content

final version approval

Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

http://orcid.org/0000-0003-4660-3045

Hari Sunarto

Conceptualization

Methodology

Formal Analysis

Investigation

Data Curation

Writing - Original Draft

Writing - Review and Editing

contribution to critical review of intellectual content

final version approval

Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

http://orcid.org/0000-0003-3214-9144

Boy Muchlis Bachtiar

Conceptualization

Methodology

Formal Analysis

Investigation

Data Curation

Writing - Original Draft

Writing - Review and Editing

contribution to critical review of intellectual content

final version approval

Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.

http://orcid.org/0000-0002-5286-2804

Correspondence: Yuniarti Soeroso, Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jl. Salemba Raya no.4, Jakarta 10430, Indonesia. E-mail: yuniarti_22@yahoo.co.id

Academic Editor: Yuri Wanderley Cavalcanti

How to cite: Maitimu FC, Soeroso Y, Sunarto H, Bachtiar BM. Association between volatile sulfur compounds Prevotella intermedia and matrix metalloproteinase-8 expression. Pesqui Bras Odontopediatria Clín Integr. 2022; 22:e210002. https://doi.org/10.1590/pboci.2022.049

Conflict of Interest

The authors declare no conflicts of interest.

Primer Name	Sequences	Reference
Prevotella intermedia	Forward: 5′-CAG CAC CCA CAA CGA TAT GA-3′ Reverse: 5′-TTC CAC CTT CTC TGC CTG TC-3′	[¹¹11 Suzuki N, Yoshida A, Nakano Y. Quantitative Analysis of multi-species oral biofilms by taqman real-time PCR. Clin Med Res 2005; 3(3):176-185. https://doi.org/10.3121/cmr.3.3.176 https://doi.org/10.3121/cmr.3.3.176... ]
16s RNA	Forward: 5′-TTA AAC TCA AAG GAA TTG ACG G-3′ Reverse: 5′-CTC ACG ACA CGA GCT GAC GAC-3′	[¹²12 Sedgley CM, Nagel AC, Shelburne CE, Clewell DB, Appelbe O, Molander A. Quantitative real-time PCR detection of oral Enterococcus faecalis in humans. Arch Oral Biol 2005; 50(6):575-83. https://doi.org/10.1016/j.archoralbio.2004.10.017 https://doi.org/10.1016/j.archoralbio.20... ]
MMP-8	Forward: 5′-GCT GCT TAT GAA GAT TTT GAC AGA G- 3′ Reverse: 5′-ACA GCC ACA TTT GAT TTT GCT TCA G-3′	[¹³13 Guan SM, Shu L, Fu SM, Liu B, Xu XL, Wu JZ. Prevotella intermedia upregulates MMP-1 and MMP-8 expression in human periodontal ligament cells. FEMS Microbiol Lett 2009; 299(2):214-22. https://doi.org/10.1111/j.1574-6968.2009.01748.x https://doi.org/10.1111/j.1574-6968.2009... ]
GAPDH	Forward: 5′-CCC AAA ACA TCA TCC CAT CTT C-3′ Reverse: 5′-GGA ACA CGG AAC GCC ATA-3′	[¹⁴14 Radonić A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun 2004; 313(4):856-62. https://doi.org/10.1016/j.bbrc.2003.11.177 https://doi.org/10.1016/j.bbrc.2003.11.1... ]

Variables	Median [Min–Max]	p-value
Methyl Mercaptan Score
Pocket depth 4–5 mm	0.27 [0.01-2.33]
Pocket depth >5 mm	0.47 [0.01-8.88]	0.55
Hydrogen sulfide Score
Pocket depth 4–5 mm	0.80 [0.07-6.40]	0.03
Pocket depth >5mm5 mm	2.30 [0.28-23.15]

Variables	Median [Min–Max]	Normality Test	p-value
Pocket depth 4–5 mm	12.49 [0.06-1004.06]	<0.05	0.79
Pocket depth >5 mm	2.27 [0.01-6709.71]

Variables	Median [Min–Max]	Normality Test	p-value
Pocket depth 4–5 mm	5.04 [0.02-550.16]	<0.05	0.39
Pocket depth >5 mm	28.64 [1.73-566.05]

Variables	Median [Min–Max]	Normality Test	p-value
Pocket depth 4–5 mm	0.02 [0.00-0.86]	<0.05	0.29
Pocket depth >5 mm	1.33 [ 0.00-5.38]

Variables	Correlation (r)*	p-value**
Methyl Mercaptan Level
Proportion of Prevotella intermedia in GCF	-0.21	0.44
Proportion of Prevotella intermedia in Tongue Coating	0.94	0.73
MMP-8 Relative Gene Expression	-0.34	0.20
Hydrogen Sulfate Level
Proportion of Prevotella intermedia in GCF	0.04	0.88
Proportion of Prevotella intermedia in Tongue Coating	0.27	0.32
MMP-8 Relative Gene Expression	-0.37	0.16

Variables	Correlation (r)	p-value
Methyl Mercaptan Level
Proportion of Prevotella intermedia in GCF	-0.79*	0.03**
Proportion of Prevotella intermedia in Tongue Coating	-0.87*	0.01**
MMP-8 Relative Gene Expression	-0.09	0.85
Hydrogen Sulfide Level
Proportion of Prevotella intermedia in GCF	-0.93*	0.003**
Proportion of Prevotella intermedia in Tongue Coating	-0.64*	0.12
MMP-8 Relative Gene Expression	-0.14*	0.76

[1] Correspondence: Yuniarti Soeroso, Department of Periodontics, Faculty of Dentistry, Universitas Indonesia, Jl. Salemba Raya no.4, Jakarta 10430, Indonesia. E-mail: yuniarti_22@yahoo.co.id

Brasil

Brasil

Association between Volatile Sulfur Compounds Prevotella intermedia and Matrix Metalloproteinase-8 Expression

Abstract

Objective:

Material and Methods:

Results:

Conclusion: