Humic acids induce the expression of nitrate transporters in passion-fruit seedlings

Ácidos húmicos induzem a expressão dos transportadores de nitrato em mudas de maracujazeiro

Abstract

The management of organic fertilization in passion fruit has been carried out empirically based on the responses of the crops and the experience of farmers. Knowledge of the physiological responses of plants to organic fertilizers is essential to optimize fertilization programs. The objective of this study was to evaluate the differential expression of genes coding for nitrate transporters as well as plasma membrane H+-ATPase in passion fruit seedlings treated with different organic fertilizers in the presence or not of humic acids. The equivalent of one gram of total nitrogen dissolved in water from cattle manure, castor oil cake and urea were added and the seedlings were treated or not with a suspension of humic acids (2 mmol/L). Differential expression of low and high affinity nitrate transporters (PeNRT2.2 and PeNRT1.1 PeNRT2.2e) and H+-ATPase (PeMha) was performed by qRT-PCR. The use of cattle manure increased the differential expression of the high affinity transporter regarding to the control. The application of humic acids amplified the increase observed in the differential transcription of the high-affinity transporter with manure and urea fertilization, with no effect observed with the use of castor oil cake. Humic acids induced transcription of the gene encoding H+-ATPases in all treatments compared to control. The application of biofertilizer based on humic acids in low concentrations directly on the plant can be used with the objective of optimizing the organic fertilization of passion fruit with cattle manure and urea.

Index terms
organic fertilizers; physiological responses; NRT genes; H+-ATPase