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In vitro rooting of Prunus rootstocks

In Prunus sp. micropropagation of rooting is considered a critical stage, since it determines the plant survival during the acclimatization. Among important factors associated with rooting, the genotype and the auxins are considered important in the induction and formation of roots. The objective of the present work was to evaluate the effect of different IBA on the in vitro rooting of Prunus rootstocks Capdeboscq and GF677, and the selections VP411 and VP417. For the in vitro rooting stage, shoots of 2-3cm long were inoculated in Lepoivre's medium supplemented with IBA (0.1, 0.5, 1.0 and 2.0 mg.L-1). The results showed that the rootstock Capdeboscq presented the highest in vitro rooting rate as well as root number per shoot. The level of 1.0 mg.L-1 of IBA was associated with rooting rates of 100%, 64% and 64% for the rootstocks Capdeboscq, GF677 and VP411, respectively. The level of 2.0 mg.L-1 of IBA was associated with rooting rates of 64.0% for the selection VP417. For the rootstocks Capdeboscq and GF677, the highest root number per shoot was 9.6 and 5.2, respectively in response to the level 2.0 mg.L-1 of IBA. As for the selections VP411 and VP417 the highest root number (3.6 and 3.9; respectively) was observed in response to the level 1.0 mg.L-1 of IBA.

Peach tree; IBA; rhizogenesis; micropropagation; genotype


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