ABSTRACT
The goal of the present study is to assess the physiological potential of Byrsonima crassifoliaseeds and analyze the procedures of the germination and tetrazolium tests, as well as the conservation of such seeds by testing different combinations of packaging and environment. The experiment used pyrenes of the clone Açu, collected from mother plants in the germplasm collection of muruci plants of Embrapa Amazonia Oriental, in Belém, Brazil. Different temperatures were tested in the germination test (25, 30, 35 and 20 - 30 ºC). The best methodology and assessment dates were determined for the test, and the seedlings of the species were characterized. Viability was assessed with the tetrazolium test, with combinations of hydration methods (by immersion and between paper towels), staining periods (3 and 4 h) and concentrations of tetrazolium solution (0.5, 0.7 and 1.0%). Storage of muruci pyrenes was evaluated at three, six and twelve months, in polyethylene and Kraft paper bags, under dry chamber and refrigerator conditions. The study used a randomized block design with four replicates. The data underwent analysis of variance. In conclusion, the physiological potential of seeds of B. crassifoliacan be assessed by the germination test conducted in sand, with alternating temperatures of 20-30 ºC, or with the use of the tetrazolium test (with staining for three hours in the tetrazolium solution at 1.0%) as a rapid assessment of viability. Muruci pyrenes can be kept in polythene or paper Kraft bags for up to 12 months, in a dry chamber at 16 ºC and with relative air humidity of 50 to 60%.
Index terms
Pyrene; germination; tetrazolium test; storage
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