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Feeding preference of Dione juno juno (CRAMER, 1779) (Lepidoptera: Nymphalidae) to passion fruit genotypes

This aim of this study was to evaluate the effect of passion fruit genotypes on the attractiveness and feeding non-preference of D. juno juno larvae, through free-choice and no-choice tests. The experiments were carried out at FCAV/UNESP in Jaboticabal-SP, under lab conditions (T=26 ± 1°C, RH = 60 ± 10% and photophase = 14 hours), with Passiflora edulis, P. gibertii, P. alata, Sul Brasil, IAC-275, Flora FB 300, P. serrato-digitata, P. edulis f. flavicarpa, Maguary FB-100 and P. foetida genotypes. In free-choice test, Petri dishes with leaf disks (3.2 cm) of each genotype were used and distributed at equal distance from the center, and 5 new hatched larvae or one 10-day old larva per genotype were released. In choice test was placed only one leaf disk of each genotype per dish (9 cm in diameter) at the same infestation utilized in free-choice test. Evaluations were carried out in two stages, being first verified the attractiveness, by counting the number of insects on each disk at 1, 3, 5, 10, 15, 30, 60, 120, 240 minutes and 24 hours after releasing. In the second stage, leaf consumption was measured 24 hours after beginning the test. P. alata was the least attractive genotype to both new-hatched and 10-day old larvae in free-choice and no-choice tests. P. alata and P. foetida were the least consumed, to both larvae stages.

Passiflora spp.; host plant resistance; juno silverspot


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