Peripheral blood smear presenting numerous platelets adhered to mature granulocytes consistent with platelet satellitism
Platelet satellitism is an in vitro phenomenon involving platelet rosetting around mature granulocytes that is observed exclusively in edetic acid (EDTA)-treated peripheral blood at room temperature(11. Kjeldsberg CR, Swanson J. Platelet satellitism. Blood. 1974;43(6):831-6.). The precise underlying mechanism of platelet satellitism remains to be fully elucidated but the current explanations include an immunologic mechanism, characterized by EDTA-dependent binding of serum IgG antibodies to both the platelet glycoprotein IIb/IIIa complex and neutrophil Fcg-receptors(22. Bizzaro N, Goldschmeding R, von dem Borne AE. Platelet satellitism is Fc gamma RIII (CD16) receptor-mediated. Am J Clin Pathol. 1995;103(6):740-4.); and a non-immunologic adherence mediated by thrombospondin or another alpha-granule protein(33. Christopoulos C, Mattock C. Platelet satellitism and alpha granule proteins. J Clin Pathol. 1991;44(9):788-9.).
The main clinical significance of this event lies in false low automated platelet counts (pseudothrombocytopenia) and highlights the importance of peripheral blood smear analysis in patients with thrombocytopenia. The collection of a new blood sample in the S-Monovette ThromboExact tube (Sarsted, Germany) or alternatively in citrate or heparin can solve this issue.
References
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1Kjeldsberg CR, Swanson J. Platelet satellitism. Blood. 1974;43(6):831-6.
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2Bizzaro N, Goldschmeding R, von dem Borne AE. Platelet satellitism is Fc gamma RIII (CD16) receptor-mediated. Am J Clin Pathol. 1995;103(6):740-4.
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3Christopoulos C, Mattock C. Platelet satellitism and alpha granule proteins. J Clin Pathol. 1991;44(9):788-9.
Publication Dates
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Publication in this collection
2013
History
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Received
24 Apr 2013 -
Accepted
17 May 2013
