ABSTRACT
The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by separation of fragments obtained from digested PCR products in 3% agarose gel electrophoresis. AA genotype (HaeIII) and BB genotype (RsaI) of exon 1 and only AA genotype (RsaI) of exon 3 were obtained. No polymorphisms were determined in Anatolian water buffalo breed and all loci were found as monomorphic. It can be stated that Anatolian water buffalo has a higher milk and milk fat yields since BB genotype was obtained.
HaeIII; PCR-RFLP; prolactin; RsaI; Sivas
PCR - polymerase chain reaction; PRL - prolactin; bp - base pair.857 bp band; M for 100 bp Ladder.
PCR - polymerase chain reaction; PRL - prolactin; bp - base pair.156 bp band; M for 100 bp Ladder.
PRL - prolactin; bp - base pair.A: HaeIII digestion; samples with AA genotype; M for 100 bp Ladder.B: RsaI digestion; samples with BB genotype; M for 100 bp Ladder.
PRL - prolactin; bp - base pair.1 to 16: samples with AA genotypes (156 bp); M for 100 bp Ladder.