Genetic diversity and primary resistance among HIV-1-positive patients from Maringá, Paraná, Brazil

Diversidade genética e resistência primária entre pacientes HIV-1-positivos de Maringá, Paraná, Brasil

Karine Vieira Gaspareto Flávia Myrian Martins de Almeida Mello José Ricardo Colleti Dias Vera Alice Fernandes Meneguetti Marta Evelyn Giansante Storti João Leandro de Paula Ferreira André Minhoto Lança Rosângela Rodrigues Luis Fernando de Macedo Brígido Jorge Juarez Vieira Teixeira Dennis Armando Bertolini About the authors

The objective of this study is to identify subtypes of Human Immunodeficiency Virus type 1 (HIV-1) and to analyze the presence of mutations associated to antiretroviral resistance in the protease (PR) and reverse transcriptase (RT) regions from 48 HIV-1 positive treatment naïve patients from an outpatient clinic in Maringá, Paraná, Brazil. Sequencing was conducted using PR, partial RT and group-specific antigen gene (gag) nested PCR products from retrotranscribed RNA. Transmitted resistance was determined according to the Surveillance Drug Resistance Mutation List (SDRM) algorithm. Phylogenetic and SimPlot analysis of concatenated genetic segments classified sequences as subtype B 19/48 (39.6%), subtype C 12/48 (25%), subtype F 4/48 (8.3%), with 13/48 (27.1%) recombinant forms. Most recombinant forms were B mosaics (B/F 12.5%, B/C 10.4%), with one C/F (2.1%) and one complex B/C/F mosaic (2.1%). Low levels of transmitted resistance were found in this study, 2/48 (2.1% to NRTIs and 2.1% for PI). This preliminary data may subsidize the monitoring of the HIV evolution in the region.

Primary resistance; Transmitted resistance; HIV-1; Naïve patients; Subtypes; Brazil

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