Isolation of urease-positive Vibrio parahaemolyticus from diarrheal patients in Northeast Brazi

Magalhães, Marcelo; Magalhães, Vera; Antas, Maria G.; Tateno, Seiki

doi:10.1590/S0036-46651991000400004

Abstracts

Of 21 human fecal strains of Vibrio parahaemolyticus, isolated on the Northeast Coast of Brazil, eight (38%) were urease positive. Most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the Kanagawa phenomenon.

Vibrio parahaemolyticus; Vibrio diarrhea; Vibrio urease; Kanagawa phenomenon

De 21 linhagens de Vibrio parahaemolyticus, isoladas de fezes humanas, na costa Nordeste do Brasil, oito (38%) foram urease positivas. A maioria dessas linhagens, em contraste com as urease-negativas, não produziram a hemolisina responsável pelo fenômeno Kanagawa.

ORIGINAL ARTICLES

Isolation of urease-positive Vibrio parahaemolyticus from diarrheal patients in Northeast Brazil

Vibrio parahaemolyticus urease-positivos de pacientes diarréicos no Nordeste do Brasil

Marcelo Magalhães^I; Vera Magalhães^II; Maria G. Antas^I; Seiki Tateno^I

^ILaboratório Keizo Asami, Universidade Federal de Pernambuco. Recife, PE, Brasil

^IIDepartamento de Medicina Tropical, Universidade Federal de Pernambuco. Recife, PE, Brasil

Address for correspondence

SUMMARY

Of 21 human fecal strains of Vibrio parahaemolyticus, isolated on the Northeast Coast of Brazil, eight (38%) were urease positive. Most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the Kanagawa phenomenon.

Key words: Vibrio parahaemolyticus; Vibrio diarrhea; Vibrio urease; Kanagawa phenomenon.

RESUMO

De 21 linhagens de Vibrio parahaemolyticus, isoladas de fezes humanas, na costa Nordeste do Brasil, oito (38%) foram urease positivas. A maioria dessas linhagens, em contraste com as urease-negativas, não produziram a hemolisina responsável pelo fenômeno Kanagawa.

Full text available only in PDF format.

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Address for correspondence:

M. Magalhães

Rua Sete de Setembro, 508

CEP 50050, Recife, Brasil

Recebido para publicação em 21/11/1990

Aceito para publicação em 8/4/1991

Supported by CNPq - grant 416241 and Japan International Cooperation Agency.

1. ABBOTT, S.L.; POWERS C.; KAYSNER, C.A.; TAKEDA, Y.; ISHIBASHI, M.; JOSEPH, S.W. & JANDA, M. - Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause of Vibrio-associated gastroenteritis on the West Coast of the United States and Mexico. J. clin. Microbiol., 27: 2891-2893, 1989.
2. BLAKE, P.A.; WEAVER, R.E. & HOLLIS, D.G. - Diseases of humans (other than cholera) caused by vibrios. Ann. Rev. Microbiol., 34: 341-367, 1980.
3. FARMER III, J.J.; HICKMAN-BRENNER, F.W. & KELLY, M.T. Vibrio, In: LENNETTE, E.H.; BALOWS, A.; HAUSLER Jr. W.J. & SHADOMY, H.J., ed. Manual of clinical microbiology. 4th. ed. Washington, American Society for Microbiology, 1985. p. 282-301.
4. FISHBEIN, M. & WENTZ, B. - Enumeration, laboratory identification, and serotypic analyses of Vibrio parahacmolyticus, In: SHLESSINGER, D., ed. Microbiology 1974. Washington, American Society for Microbiology, 1975. p. 246-256.
5. FUJINO, T.; OKUNO, Y.; NAKADA, D.; AOYAMA, A.; FUKAI, K.; MUKAI, T. & UEHO, T. - On the bacteriological examination of shirasu food poisoning. Med. J. Osaka Univ., 4: 299-304, 1953.
6. HOFER, E. - Primeiro isolamento e identificaçăo de Vibrio parahacmolyticus no Brasil de infecçăo gastrintestinal humana. Rev. Microbiol. (S. Paulo), 14: 174-175, 1983.
7. HUQ, M.I.; HUBER, D. & KIBRYIA, G. - Isolation of urease producing Vibrio parahacmolyticus strains from cases of gastroenteritis. Indian J. med. Res.,70: 549-553, 1979.
8. KELLY, M.T. & STROH, E.M.D. - Urease-positive, Kanagawa negative Vibrio parahacmolyticus from patients and the environment in the Pacific Northwest. J. clin. Microbiol., 27: 2820-2822, 1989.
9. LAM, s. & YEO, M. - Urease-positive Vibrio parahacmolyticus strain. J. clin. Microbiol., 12: 57-59, 1980.
10. NOLAN, C.M.; BALLARD, J.; KAYSNER, C.A.; LILJA, J.L.; WILLIAMS Jr, L.P. & TENOVER, F.C. - Vibrio parahacmolyticus gastroenteritis: an outbreak associated with raw oysters in Pacific Northwest. Diagn. Microbiol. infect. Dis., 2: 119-128, 1983.
11. OBERHOFER, T.R. & PODGORE, J.K. - Urea-hydrolysing Vibrio parahacmolyticus associated with acute gastroenteritis. J. clin. Microbiol., 16: 581-583, 1982.
12. SAKAZAKI, R.S.; IWANAMI, S. & FUKUMI, H. - Studies on the enteropathogenic facultatively halophilic bacteria, Vibrio parahacmolyticus I. Morphological, cultural and biochemical properties and its taxonomical position. Jap. J. med. Sci. Biol., 16: 161-188, 1963.
13. WACHSMUTH, I.K.; DAVIES, B.R. & ALLEN, S.D. - Ureolytic Escherichia coli of human origin: Serological, epidemiological, and genetic analysis. J. clin. Microbiol., 10: 897-902, 1979.

Address for correspondence: M. Magalhães Rua Sete de Setembro, 508 CEP 50050, Recife, Brasil

Publication Dates

Publication in this collection
12 Sept 2006
Date of issue
Aug 1991

History

Accepted
08 Apr 1991
Received
21 Nov 1990

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ABBOTT, S.L.; POWERS C.; KAYSNER, C.A.; TAKEDA, Y.; ISHIBASHI, M.; JOSEPH, S.W. & JANDA, M. - Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause of Vibrio-associated gastroenteritis on the West Coast of the United States and Mexico. J. clin. Microbiol., 27: 2891-2893, 1989.

[2] 2. BLAKE, P.A.; WEAVER, R.E. & HOLLIS, D.G. - Diseases of humans (other than cholera) caused by vibrios. Ann. Rev. Microbiol., 34: 341-367, 1980.

[3] 3. FARMER III, J.J.; HICKMAN-BRENNER, F.W. & KELLY, M.T. Vibrio, In: LENNETTE, E.H.; BALOWS, A.; HAUSLER Jr. W.J. & SHADOMY, H.J., ed. Manual of clinical microbiology. 4th. ed. Washington, American Society for Microbiology, 1985. p. 282-301.

[4] 4. FISHBEIN, M. & WENTZ, B. - Enumeration, laboratory identification, and serotypic analyses of Vibrio parahacmolyticus, In: SHLESSINGER, D., ed. Microbiology 1974. Washington, American Society for Microbiology, 1975. p. 246-256.

[5] 5. FUJINO, T.; OKUNO, Y.; NAKADA, D.; AOYAMA, A.; FUKAI, K.; MUKAI, T. & UEHO, T. - On the bacteriological examination of shirasu food poisoning. Med. J. Osaka Univ., 4: 299-304, 1953.

[6] 6. HOFER, E. - Primeiro isolamento e identificaçăo de Vibrio parahacmolyticus no Brasil de infecçăo gastrintestinal humana. Rev. Microbiol. (S. Paulo), 14: 174-175, 1983.

[7] 7. HUQ, M.I.; HUBER, D. & KIBRYIA, G. - Isolation of urease producing Vibrio parahacmolyticus strains from cases of gastroenteritis. Indian J. med. Res.,70: 549-553, 1979.

[8] 8. KELLY, M.T. & STROH, E.M.D. - Urease-positive, Kanagawa negative Vibrio parahacmolyticus from patients and the environment in the Pacific Northwest. J. clin. Microbiol., 27: 2820-2822, 1989.

[9] 9. LAM, s. & YEO, M. - Urease-positive Vibrio parahacmolyticus strain. J. clin. Microbiol., 12: 57-59, 1980.

[10] 10. NOLAN, C.M.; BALLARD, J.; KAYSNER, C.A.; LILJA, J.L.; WILLIAMS Jr, L.P. & TENOVER, F.C. - Vibrio parahacmolyticus gastroenteritis: an outbreak associated with raw oysters in Pacific Northwest. Diagn. Microbiol. infect. Dis., 2: 119-128, 1983.

[11] 11. OBERHOFER, T.R. & PODGORE, J.K. - Urea-hydrolysing Vibrio parahacmolyticus associated with acute gastroenteritis. J. clin. Microbiol., 16: 581-583, 1982.

[12] 12. SAKAZAKI, R.S.; IWANAMI, S. & FUKUMI, H. - Studies on the enteropathogenic facultatively halophilic bacteria, Vibrio parahacmolyticus I. Morphological, cultural and biochemical properties and its taxonomical position. Jap. J. med. Sci. Biol., 16: 161-188, 1963.

[13] 13. WACHSMUTH, I.K.; DAVIES, B.R. & ALLEN, S.D. - Ureolytic Escherichia coli of human origin: Serological, epidemiological, and genetic analysis. J. clin. Microbiol., 10: 897-902, 1979.

Brazil

Brazil

Isolation of urease-positive Vibrio parahaemolyticus from diarrheal patients in Northeast Brazi

Vibrio parahaemolyticus urease-positivos de pacientes diarréicos no Nordeste do Brasil

Abstracts

Publication Dates

History