Abstract
Introduction
Composite resins are restorative alternatives, but their surface may favor the accumulation of biofilm.
Objective
The aim of this study was to evaluate the in vitro adhesion of Streptococcus mutans (UA159) and Candida albicans (ATCC 90028) biofilms on the surface of conventional and bulk fill composites.
Material and method
Four brands of conventional and bulk fill composites were used, Aura Bulk Fill - SDI®, Premisa - Kerr®, Opallis- FGM® and Filtek bulk fill flow - 3M®. Artificial saliva was used to form the salivary film for 60 min at 37 ° C. The inoculum was standardized at 1x108 CFU/mL for S. mutans and 1x106 CFU/mL for C. albicans. The specimens (n = 8/group) were placed in 24-well plates, with BHI supplemented with sucrose for bacteria and RPMI 1640 for fungi. The biofilm formation was evaluated considering the colony forming units (CFU/mL). The data were analyzed by ANOVA and Tukey test (p <0.05).
Result
For S. mutans biofilms, there was no significant difference in the CFU/mL count between the different types of composites (p = 0.119). In the CFU / mL count for C.albicans biofilm, the CFU/mL ranged from 7.78 to 8.34. There was a significant difference between brands for Candida, especially between conventional and bulk fill composites.
Conclusion
The present study demonstrates that there is no difference in adhesion for S. mutans biofilms. On the other hand, there is a difference in the adhesion of C. albicans to the surface of different composite resins.
Descriptors:
Streptococcus mutans; Candida albicans; dental restoration, permanent; dental materials
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