ABSTRACT
Through a long-term micro-area positioning experiment (2006-2017), the precise fertilization locations of spring maize (Zea mays L.) in Jilin, China, were investigated using an embedded cement bucket pot. The effects of different fertilization placements and additional application of organic manure on maize yield, biomass, grain nutrient uptake, and soil fertility were examined. Six treatments were designed: base fertilizer + topdressing shallow application (BF1TD1); base fertilizer deep + topdressing shallow application (BF2TD1); base fertilizer + topdressing deep application (BF1TD2); base fertilizer deep + topdressing deep application (BF2TD2); base fertilizer + topdressing + organic manure shallow application (BF1TD1 + OS); and base fertilizer + topdressing shallow application + organic manure deep application (BF1TD1 + OD). After 12 years of continuous experimentation, the findings revealed that BF2TD2, BF1TD1 + OS, and BF1TD1 + OD exhibited 10.59 %, 25.17 %, and 29.34 % higher average yields than BF1TD1, respectively. Deep topdressing was more beneficial in increasing maize yield and nutrient accumulation, and additionally, increasing the use of organic fertilizer enhanced plant biomass and nutrient uptake. Over the 12 years considered, the soil organic matter in the 0-20 cm and 20-40 cm layers increased by 45.96 % and 80.61 %, respectively, and the soil pH increased from 0.38 to 0.48. In general, the deep application of organic manure was more beneficial for soil retention as it can increase soil fertility in the 0-40 cm layer. Considering the high yield and nutrient absorption and utilization of maize, technical advancement for soil fertilizer in whole-field tillage layers was crucial.
NPK; maize yield; nutrient absorption; pH; soil nutrient
Introduction
Agricultural practices incur environmental costs, including greenhouse gases (Hou et al., 2021; Liu et al., 2022), ozone layer destruction (Jiao et al., 2022), groundwater pollution (Megdal, 2018), acid rain (Debnath et al., 2018), and biodiversity loss (Fanin et al., 2018). To promote sustainable yield and reduce environmental costs, Chinese smallholder farmers have utilized improved management practices (Cui et al., 2018) and increased organic fertilizer usage. However, the expenses and requirements for a larger labor force have reduced the enthusiasm of farmers (Cai et al., 2018). Chemical fertilizers increase crop yield in the short term, but they barely sustain soil fertility and even reduce it (Jiang et al., 2018; Schjoerring et al., 2019; Du et al., 2021; Grandy et al., 2022). In crop fertilizer management technologies, the fertilization site is as crucial as the quantity and ratio of fertilization (Saïdou et al., 2018; Lu et al., 2019). Different application placements can affect soil and plant nutrient uptake (Nguyen et al., 2018; Yong et al., 2018). Fertilizer application at a depth of 15-20 cm has been found to play a crucial role in increasing crop nutrient and nitrogen (N) utilization rate, enhancing soil N supply capacity, and promoting dry matter accumulation and N absorption in China (Ke et al., 2018; Mi et al., 2018b). Long-term fertilizer location tests can provide scientific guidance for fertilization (Zhang et al., 2018). Most of the above studies focused on the fertilizer application location in a specific period (Liu et al., 2019) or on the type of fertilizer (Zhang et al., 2019c). Mechanized fertilizer application has become common recently (Yang et al., 2018). It is crucial to consider the effect of fertilizer application location on maize growth and nutrient uptake during the key fertilizer application periods in the growing season.
Modern agriculture has greatly popularized mechanical fertilizer applications. It is crucial to investigate the effect of fertilizer application sites on maize growth and nutrient uptake during the two critical fertilizer application phases in the maize growing seasons, the pre-sowing and jointing stages. This research aimed to enhance and automate agronomic methods for accurate maize fertilization to ensure food security, environmentally friendly agricultural practices, and the sustainable utilization of organic fertilizer. To achieve this, the study conducted 12-year cement bucket experiments using pots to systematically examine the effects of various fertilizer application placements and organic manure fertilizer application on maize yield, plant nutrient accumulation, and soil nutrients in the black soil region of central northeast China, and to provide scientific guidance for fertilization in this region, support improvement in fertilizer application machinery, and establish a scientific foundation for enhancing farmland productivity of black soil areas.
Materials and Methods
Overview of the study area
The ongoing field experiment commenced in the spring of 2006, in Gongzhuling City, Jilin Province, northeast China (43°29’55” N, 124°48’43” E, altitude 120 m). The region is characterized by a temperate continental monsoon climate featuring cold winters, high temperatures, and rainy summers. The average annual precipitation is 600 mm, and it has had an average rainfall of 477.38 mm from May to Sept over the past 20 years. The average annual temperature is 5.6 °C, the frost-free period lasts 125-140 days, and the effective accumulated temperature ranges from 26 °C to 30 °C. Prior to the experiment, soil samples were collected from a depth of 0-40 cm in the nursery on 28 Apr 2006. The experimental field was a typical black soil according to the Chinese Soil Classification System, classified as a fine-silty, mixed mesic typical Hapludolls according to the United States Department of Agriculture classification system (USDA). The soil texture was 31.6% clay, 39.5% silt, and 28.9% sand and the 0-20 cm layers were characterized by pH 6.10, 29.8 g kg1organic matter, 138.82 mg kg1available N, 57.94 mg kg1available phosphorus (AP), 192.85 mg kg1available potassium (AK) in the 0-20 cm layers. The 20-40 cm layers were characterized by pH 6.79, 22.0 g kg1organic matter, 125.41 mg kg1available N, 32.70 mg kg1AP, and 170.43 mg kg1AK.
The monthly rainfall data during the maize growth period from 2006 to 2017 are shown in Figure 1. In this study, the annual rainfall years were categorized according to the dryness index (DI) (Liu et al., 2021). DI was calculated using the formula DI = (P-M)/σ, DI < −0.35 indicates a drought year, −0.35 ≤ DI ≤ 0.35 indicates a typical water year, DI > 0.35 indicates a wet year, P represents the rainfall during the maize growth period of the current year (mm), and M the average rainfall during the multi-year maize growth period (mm) (May-Sept). In the drought years of 2006, 2007, 2009, 2011, 2014, and 2015, average precipitation during the maize growth period was 334.0 mm, and DI was –1.11. Notably, the precipitation in 2014 was 420.2 mm, and the rainfall during the growth period from May to Sept was relatively uniform. Average rainfall during the growth period of maize in 2017 was 513.0 mm, and DI was 0.30, which indicates a typical water year. Rainfall was evenly distributed in May, June, and Sept, while the flowering and grain-filling periods of maize were from July to Aug. In the years of abundant rainfall, including 2008, 2010, 2012, 2013, and 2016, average precipitation during the maize growth period was 571.4 mm, and DI was 0.73, which indicates a wet year. In 2010, the maize jointing stage experienced drought conditions, while 2012-2013 was characterized by less rainfall during the maize seedling stage.
Experimental design
The experiment involved in situ potted plants, with soil embedded in hollow cement buckets measuring 70 cm in diameter, covering an area of 0.385 m2. The variety tested was Xianyu 335, and three maize seeds were sown in each pot, with a density equivalent to 78,000 plants ha1. The experimental design consisted of two factors: base fertilizer position and topdressing position. For each factor, two application depths were used: shallow (10 cm away from the upper edge of the cement bucket) and deep (30 cm away from the upper edge of the cement bucket). Additionally, organic manure was applied in both the shallow application (OS) and the deep application (OD), resulting in a total of six treatments, and each treatment was repeated three times. The base fertilizer was applied before sowing, with N fertilizer at a rate of 110 kg ha1(5.0 g urea per pot), P-based fertilizer (P2O5) at a rate of 138 kg ha1(11.4 g diammonium phosphate per pot), and K-based fertilizer (K2O) 126 kg ha1(8.0 potassium chloride g per pot). Topdressing was conducted at the corn jointing stage, with N applied at a rate of 250 kg ha1 (21 g urea per pot) and organic fertilizer at 30000 kg ha1 (1200 g per plant), applied together with the basal fertilizer. The root stubble was left in the field every autumn. The N fertilizers used were all urea (N 46 %), the phosphate fertilizers all diammonium phosphate (N 18 %, P2O5 46 %), and the K fertilizers potassium chloride (K2O 60 %). The pH, soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), and total potassium (TK) in the organic fertilizer (cattle manure) were 8.9, 302, 20.3, 5.3, and 9.7 g kg1, respectively. The seeds were sown in early May and harvested in late Sept. The actual shot and schematic diagram of the pot experiment are shown in Figure 2, and the details of the experimental treatment are in Table 1.
Sample collection and determination
Soil samples at depths of 0-20 cm and 20-40 cm were collected before sowing maize in 2006 to measure SOM, AN, AP, TK, and pH values. After the maize harvest in 2008 and 2016, soil samples at depths of 0-20 cm and 20-40 cm were collected. The following parameters were determined: soil organic matter (SOM); total nitrogen (TN); total phosphorus (TP) and total potassium (TK); available N (AN); available phosphorus (AP); available potassium (AK); and pH values. SOM was determined via the concentrated sulfuric acid-potassium dichromate elimination-ferrous sulfate titration method. TN by the semi-micro Kjeldahl method, TP via sodium hydroxide melting and the molybdenum-antimony anti-colorimetric method, TK via sodium hydroxide fusion-flame photometry, AN via the alkaline diffusion method, AP via sodium bicarbonate extraction and molybdenum-antimony anti-colorimetric method, AK via ammonium acetate extraction and flame photometry. The pH value of soil (water:soil = 2.5 mL:1.0 g) was measured using a composite electrode (Bao, 2000). At the mature stage of maize, all three plants in each treatment and pot were harvested, and the grain yield (calculated using drying mass) and 100-kernel weight of each plant were determined. After the harvests from 2013 to 2015, the leaves, stems (sheaths), grains, and cob organs of the plants were separated. The plants were then dried in an oven at 105 °C for 60 min, then at 75 °C until constant weight was achieved. The dried plants were crushed to determine the contents of N, P, and K via the semi-micro Kjeldahl method for TN, the sodium hydroxide fusion-molybdenum-antimony anti-colorimetric method for TP, and the sodium hydroxide fusion-flame photometry was used for TK (Bao, 2000).
Statistical analysis
Statistical analysis was conducted with SPSS 22.0 software. One-way analysis of variance and multiple comparisons (Duncan’s method) were performed. Data processing and graphing were conducted using Microsoft Excel 2016.
Results
Influences of different fertilization placements on maize yield and its composition
From 2006 to 2017, the average yearly yields of the five treatments (BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD) were 304.6, 321.9, 325.8, 368.7, and 381.0 g per plant, respectively (Figure 3). Compared with the BF1TD1 treatment, the yields increased by 3.40 %, 9.28 %, 10.59 %, 25.17 %, and 29.34 %, respectively. Over the 12 years considered, the BF1TD1 + OS and BF1TD1 + OD treatments had the highest yields, followed by the BF2TD2 treatment. In the early stage, from 2006 to 2008, the yield of the BF1TD1 treatment was only marginally higher than that of the BF2TD1 treatment. The yields of the six treatments in the first three years differed slightly (p > 0.05). Since 2009, the order of maize yield has been BF2TD2 > BF1TD2 > BF2TD1 > BF1TD1, which indicates that different fertilizer application placements directly impact fertilizer efficiency and crop yield levels. Throughout the 12 years, the application of organic fertilizer increased owing to the shallow fertilizer application. Only the yields in 2006, 2010, and 2011 demonstrated that BF1TD1 + OS > BF1TD1 + OD. In other years, the yield of deep application of organic manure was superior to that of shallow application. This illustrates that using organic manure can increase maize output, with deep fertilization partially important. The yield contained a sizeable portion of the 100-kernel weight. The average yearly 100-kernel weights of the fertilization positions for BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD were higher than that of the BF1TD1 treatment, by 0.91 %, 1.96 %, 3.20 %, 4.90 %, and 7.87 %, respectively. The consistent yields further demonstrated the effectiveness of deep fertilization (Figure 4). The yearly rainfall pattern determined by DI can be used to assess maize production and its yield composition (Table 2). In the drought years, the yield of BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 0.58 %, 5.07 %, 8.06 %, 22.05 %, and 27.46 %, respectively, and the 100-kernel weight of BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 0.76 %, 3.59 %, 4.07 %, and 6.81 %, respectively, compared with the BF1TD1 treatment. In plain water years, the yields of BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 5.58 %, 14.07 %, 14.48 %, 36.18 %, 37.96 % and the 100-kernel weight of BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 2.28 %, 5.01 %, 5.47 %, 7.11 %, 9.85 % compared with the BF1TD1 treatment. In wet water years, the yields of BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 3.51 %, 7.61 %, 8.41 %, 15.19 %, and 21.21 %, while the 100-kernel weight of BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 0.47 %, 0.22 %, 0.70 %, 3.60 %, and 7.01 %, respectively, compared with the BF1TD1 treatment. The treatment, the year, and their interactions substantially influenced the yield and 100-kernel weight (Table 2). Multiple applications of organic manure, particularly deep applications, greatly improved yield and composition compared with single applications of inorganic fertilizer.
– Maize yield and its increase rate under different fertilizer placements from 2006 to 2017. Values followed by different small letters in one column in the same year indicate differences between treatments at 0.05 level (Duncan’s method). The increase rate was determined by comparing BF1TD1 with other treatments.
– 100-kernel weight of different fertilizer placement from 2006 to 2017. Values followed by different small letters in one column in the same year indicate differences between treatments at 0.05 level (Duncan’s method).
Impacts of different fertilization placements on plant nutrient uptake and distribution
Organic fertilizer application had a considerable impact on maize biomass (Figure 5). In 2013, the biomass of maize in BF2TD1, BF1TD2, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD increased by 1.78 %, 2.00 %, 3.63 %, 9.45 % and 14.26 %, respectively, compared with the BF1TD1 treatment. The increases were 5.88 %, 7.59 %, 7.89 %, 32.71 %, and 43.43 % in 2014, and 4.27 %, 6.61 %, 8.24 %, 19.74 %, and 26.39 % in 2015, respectively, compared with the BF1TD1 treatment. In the wet year (2013), only the deep application of organic manure massively increased maize biomass (p < 0.05). In the dry years (2014, 2015), both the deep and shallow applications of organic manure increased maize biomass. The biomass of maize from 2013 to 2015 followed the order BF1TD1 + OD > BF1TD1 + OS. These findings suggest that the comprehensive application of organic manure can greatly increase soil fertility and buffering effectiveness, ensure stable high maize production in both wet and dry years, and reduce soil erosion.
– Effects of different fertilizer placement on biomass in maize from 2013 to 2015. Values followed by different small letters in one column in the same year indicate difference among treatments at 0.05 level (Duncan’s method).
Owing to nutrient absorption in grain and straw from 2013 to 2015 (Figures 6 -8), N and P predominantly accumulated in the grains, accounting for 70.2 %-80.8 % of the total N accumulation and 60.0 %-67.1 % of the total P accumulation, respectively. There was no substantial difference in N and P accumulation in straw (p > 0.05). However, most of the K accumulated in straw, and the K in grain accounted for 18.1 %-32.6 % of the total K accumulation. Compared with BF1TD1, the N uptake of the BF2TD1, BF1TD2, and BF2TD2 treatment respectively increased by 2.35 %, 3.34 %, and 4.91 %, P uptake by 2.30 %, 4.20 %, and 6.12 %, and K uptake by 3.31 %, 5.51 %, and 7.60 %. Compared with the BF1TD1 treatment, the BF1TD1 + OS treatment featured N, P, and K uptake of 21.63 %, 22.94 %, and 35.15 %, respectively, while the BF1TD1 + OD treatment featured N, P, and K uptake of 30.11 %, 29.34 %, and 48.89 %, after the application of organic manure. From the perspective of different fertilization placements, the grain N, P, and K uptake showed the following trend BF2TD2 > BF1TD2 > BF2TD1 > BF1TD1, which indicates that the deep application of basal and topdressing fertilizer could increase nutrient uptake.
– Effects of different fertilizer placement on nitrogen absorption in maize from 2013 to 2015. Values followed by different small letters in one column in the same year indicate difference among treatments at 0.05 level (Duncan’s method).
– Effects of different fertilizer placement on phosphorus absorption in maize from 2013 to 2015. Values followed by different small letters in one column in the same year indicate difference among treatments at 0.05 level (Duncan’s method).
– Effects of different fertilizer placement on potassium absorption in maize from 2013 to 2015. Values followed by different small letters in one column in the same year indicate difference among treatments at 0.05 level (Duncan’s method).
Compared with the BF1TD1, in 2013, the grain N uptake of the BF1TD2, BF2TD1, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD treatments respectively increased by 5.90 %, 5.41 %, 6.14 %, 14.50 %, and 23.42 %, grain P uptake by 0.27 %, 1.85 %, 3.61 %, 13.12 %, and 18.93 %, and grain K uptake by 4.08 %, 3.54 %, 4.82 %, 46.29 %, and 48.31 %. In 2014, compared with BF1TD1 treatment, the grain N uptake of the BF1TD2, BF2TD1, BF2TD2, BF2TD2, and BF1TD1 + OD treatments respectively increased by 1.92 %, 3.27 %, 6.91 %, 25.54 %, and 34.21 %, grain P uptake increased by 3.30 %, 4.22 %, 7.17 %, 28.91 %, and 35.15 %, and K uptake in grain was increased by 4.72 %, 9.87 %, 13.61 %, 42.74 %, and 64.87 %, respectively. In 2015, compared with the BF1TD1 treatment, grain N uptake respectively increased by 1.92 %, 3.27 %, 6.91 %, 25.54 %, and 34.21 %, and grain P uptake by 5.31 %, 9.32 %, 9.78 %, 28.85 %, and 36.47 %. Additionally, K uptake in grain increased by 0.27 %, 1.90 %, 2.98 %, 15.66 %, and 31.40 %, respectively., Thus, the nutrient uptakes of grains of BF1TD1 + OD for the same fertilizer type were higher than BF1TD1 + OS, regardless of fertilization placement, growing season, and fertilizer type. Chemical fertilizer treatment had no appreciable effects, substantiating the fact that organic fertilizer impacted maize growth and nutrient uptake.
Influence of different fertilization placements on soil nutrient content
During a multi-year localization test, the amounts of SOM, AP, AK, TN, TP, TK, and soil pH varied dramatically depending on the treatments (Table 3). Compared with the initial values in 2006, SOM in all treatments increased by 4.36 %-106.68 %, and SOM in the 0-40 cm soil layer increased greatly in the BF1TD1 + OS and BF1TD1 + OD treatments. Moreover, compared with the initial values, for both treatments, the SOM in the 0-20 cm soil layer increased by 49.66 % and 42.25 % (average 45.96 %), and in the 20-40 cm soil layer increased by 54.55 % and 106.68 % (average 80.61 %), respectively. These results differed the other treatments (p < 0.05). Compared with the levels in 2006, only the BF1TD1 + OS and BF1TD1 + OD treatments enhanced the AN levels in the 0-40 cm soil layer, with a 35.34 % and 69.83 % increase in the 0-20 cm soil layer and a 45.79 % and 26.15 % increase in the 20-40 cm soil layer, respectively. However, the other treatments and soil layers exhibited decreases, and the differences were expressive (p < 0.05). The AP and AK content in the 20-40 cm soil layer increased by 26.30 % and 1.79 % in the BF1TD1 + OD treatment compared with the contents in 2006, while the BF1TD1, BF1TD2, BF2TD1, BF2TD2, BF1TD1 + OS, and BF1TD1 + OD treatments exhibited decreases of 10.55 %-76.30 % and 3.74 %-36.95 %, respectively. In terms of soil pH, all treatments exhibited a decrease of 0.02-0.68 units in the 0-20 cm soil layer compared with levels in 2006, while the 20-40 cm soil layer showed an increase of 0.20-0.48 units for all treatments except for BF1TD1, which had a lower value than the initial soil pH. The order of increase was BF1TD1 + OD > BF1TD1 + OS > BF2TD1 > BF1TD2.
After 11 years of the experiment, the 0-40 cm soil layer exhibited some alterations in SOM, AN, AP, AK, TN, TP, TK, and pH. Compared with the BF1TD1 treatment, the BF1TD1 + OD and BF1TD1 + OS treatments showed higher contents of SOM, AN, AP, AK, TN, and TP, with an average increase of 24.96 % and 39.68 % in SOM, and 52.25 % and 70.00 % in AN, respectively. AP increased by an average of 80.53 % and 132.48 %, while AK increased by 34.12 % and 35.84 %, respectively. The soil pH value of the BF1TD1 + OD treatment increased by 0.09 units compared with the BF1TD1 treatment, whereas the BF1TD2, BF2TD1, BF2TD2, BF1TD1 + OS treatments showed a decrease of 0.01-0.13 units.
Discussion
A practical strategy for promoting sustainable agricultural development is the “4R” nutrient management, which includes “reasonable fertilizer location” to optimize nutrient application under suitable environmental conditions, enhancing nutrient uptake by crops, and reducing losses through fixation, volatilization, and leaching (Mi et al., 2018a). Improper fertilization placement can negatively affect the yield result in terms of wastage of fertilizer resources (Waqas et al., 2020). In this study, the average yield of maize kernels over the 12 years was basal fertilizer + topdressing deep application > basal fertilizer shallow application + topdressing deep application > basal fertilizer deep application + topdressing shallow application > basal fertilizer + topdressing shallow application. Although the impact of climatic changes varied, it revealed that deep chemical fertilizer applications generally outperformed shallow applications, and the combination of deep basal fertilizer and topdressing fertilizer applications had a positive effect on maize yield. Additionally, most above-ground nutrients such as nitrogen, phosphorus, and potassium were accumulated in the grains rather than stew. The base fertilizer + top fertilizer deep application had the best effect, followed by base fertilizer shallow application + top fertilizer deep application, followed by base fertilizer deep application + top fertilizer shallow application. This indicates that deep topdressing may enhance nutrient absorption and utilization (Sileshi et al., 2019). These findings are essential for guiding robotic precision fertilization in the black soil region of northeast China.
According to the analysis of long-term fertilization experiments in China (Zhang et al., 2019b), the average yield of corn increased by 2015 kg ha1when chemical fertilizers were combined with organic fertilizers. In this research study, the fertilization method was optimized, and the maize grain yield under shallow and deep applications of organic fertilizer remained stable or showed a steady increase over the years of fertilization. In contrast, the maize yield under chemical fertilizer application alone was lower than that under the combined application of organic fertilizer and chemical fertilizer in the same year. The annual average yields of base fertilizer + topdressing + organic manure shallow application and base fertilizer + topdressing shallow application + organic manure deep application were 368.7 g per plant and 381.0 g per plant, respectively, which increased by 25.17 % and 29.34 % compared with the base fertilizer + topdressing shallow application. In 9 out of 12 years, deep application of organic manure led to higher yields than shallow application yielded. Deep application also resulted in increased biomass and accumulation of the nitrogen, phosphorus, and nutrients potassium in the grain. Growth increased by 4.39 %-8.08 %, 6.42 %-7.80 %, 4.84 %-5.91 %, and 1.38 %-15.50 %, respectively. Moreover, since 2012, the deep application of organic manure has shown a relatively stable increase in yield. These findings are consistent with Zhang et al. (2022) research and indicate that the 100-kernel weight influenced the increase in maize production. Deep application of organic manure boosted the increase of the 100-kernel weight of maize, which was 68.41 % greater than that in that shallow application. According to the DI, compared with the base fertilizer + topdressing shallow application, the yield and 100-kernel weight of the shallow and deep application of organic manure treatments increased, respectively, by 22.05 %, 27.46 %, 4.07 %, 6.81 % in the drought year, 36.18 %, 37.96 %, 7.11 %, 9.85 % in the normal-water year, and 15.19 %, 21.21 %, 3.60 %, and 7.01 % in the wet years. These findings suggest that the long-term application of deep organic manure cultivation could significantly improve maize production, ensure high yield stability, and eliminate the negative impact of inter-annual climate variations in maize yield.
The amount of organic matter in the soil is a crucial indicator of soil quality, and the presence of different nutrients in the soil greatly influences crop nutrient acquisition (Chai et al., 2019). As of 2016, each treatment made a sizable impact on environmental variables such as soil organic matter, available phosphorus, potassium, total nitrogen, total phosphorus, total potassium content, and soil pH. Compared with the baseline value from 2006, the soil organic matter content increased in each treatment with an average growth of 31.45 %. The effect of chemical fertilizer + organic manure deep application in the 20-40 cm was particularly notable, followed by chemical fertilizer + organic fertilizer shallow application, and then basal fertilizer + top dressing, which showed a slower increase. Additionally, compared with the levels in 2008, organic matter, alkali-hydrolyzed nitrogen, and total phosphorus in the 0-20 cm soil layer increased by 24.82 %, 28.95 %, and 53.06 %, respectively, and by 94.15 %, 171.67 %, and 202.63 % in the 20-40 cm soil layer after 10 years of deep application of organic manure. However, in the 0-20 cm soil layers, organic matter, total nitrogen, and total phosphorus increased by 19.89 %, 10.33 %, and 55.10 %, respectively, and in the 20-40 cm soil layer by 85.79 %, 135.24 %, and 127.50 %, respectively. Previous research has shown that extending the duration of organic fertilizer application greatly increases fertility variables such as soil organic carbon content, available nutrient supply, and enzyme activity in the topsoil, leading to a substantial increase in maize yield (Celestina et al., 2019). One of the key factors restricting the soil quality of arable land in Jilin Province is the rise in soil bulk density, particularly in the 20-40 cm soil layer, which poses a substantial barrier to deep plowing. The distribution of nutrients between the plow layer and the subsoil layer and deep fertilization can improve the soil fertility of the subsoil layer (Jankowski et al., 2018). The findings of this study further highlight that the deep application of organic manure is conducive to soil carbon sequestration and the rapid improvement of soil fertility, which is an essential technical approach to addressing the current issues of “shallow, thin, and nutrient-poor” arable land in black soil areas, achieving cultivation of the 0-40 cm full surface layer, and improving the quality of arable land.
Too low or too high soil pH will inhibit the activities of soil microorganisms and enzymes, while long-term single application of nitrogen fertilizer has been found to intensify soil acidification (Yang et al., 2019; Yu et al., 2019). Organic fertilizer input provides a carbon source for soil microbial activities and promotes soil-related enzyme activity (Zhang et al., 2019a; Yang et al., 2022). Conversely, a single application of chemical fertilizers can drastically lower soil pH (Liu et al., 2018). Applying organic fertilizer to inherently acidic soil can increase the negative charge and neutralize H+ in the soil (Dai et al., 2019). In this study, regardless of the amount of rainfall, both shallow and deep applications of organic manure considerably increased the pH value of the soil in the 0-40 cm range. The soil pH value was 0.48 units higher than the baseline in 2006, indicating a substantial reduction in soil acidification. The comprehensive application of organic fertilizer can greatly improve soil fertility and buffering effectiveness.
In summary, our results suggest that long-term fertilizer treatments for topdressing and deep application are more advantageous than conventional application methods for improving maize productivity and nutrient absorption. Additionally, the average yield increased by 14.05 %, while the nutrient uptake of nitrogen, phosphorus, and potassium increased from 6.07 % to 22.17 %. After applying organic fertilizer, the average yield improved by 27.26 %. Nitrogen, phosphorus, and potassium uptake increased from 23.98 % to 54.87 %, while the average annual increase rates of organic matter and total nitrogen in the 20-40 cm soil layer were 7.33 % and 13.95 %, respectively. The soil pH tended to be appropriate, guaranteeing a stable, high maize yield. In Oct 2019, the State Council Information Office of the People’s Republic of China issued a white paper entitled “Food Security in China” (SCIO, 2019), which highlighted a strategy for sustainable farmland use and the innovative application of agricultural technology to increase farmland productivity, and thus strengthen China’s food security. Collectively, a comprehensive application of mechanical fertilizer combined with chemical fertilizer may maintain or even enhance maize production while increasing plant nutrient absorption, soil organic matter, nitrogen, phosphorous, and potassium supplies, and improve soil acidity. Considering all factors, applying base fertilizer at a depth of 15 cm, topdressing fertilizer at a depth of 10 cm, and organic fertilizer with the current deep tillage operation can help balance high maize production, nutrient uptake, and utilization, gradually improve soil fertility, and achieve high yield and efficiency through the integrated management of soil-crop intercropping systems to achieve sub-tillage fertilization.
Acknowledgments
This research was supported by the Innovation Project of Jilin Academy of Agricultural Sciences (CXGC2021ZD001), the National Key Research and Development Program of China (2021YFD150020104), the Science and Technology Development Planning Project of Jilin Province (20220302001NC), and we acknowledge Xiuzhi Bian, Xiaogong Yan, and Hongxi Zhang, for their technical support. We are grateful to Wei Ma, for writing guidance.
References
- Bao S. 2000. Soil Agrochemical Analysis. China Agriculture Press, Beijing, China.
-
Cai Y, Yu L. 2018. Rural household participation in and satisfaction with compensation programs targeting farmland preservation in China. Journal of Cleaner Production 205: 1148-1161. https://doi.org/10.1016/j.jclepro.2018.09.011
» https://doi.org/10.1016/j.jclepro.2018.09.011 -
Celestina C, Hunt JR, Sale PWG, Franks AE. 2019. Attribution of crop yield responses to application of organic amendments: A critical review. Soil and Tillage Research 186: 135-145. https://doi.org/10.1016/j.still.2018.10.002
» https://doi.org/10.1016/j.still.2018.10.002 -
Chai Y, Zeng X, Shengzhe E, Che Z, Bai L, Su S, et al. 2019. The stability mechanism for organic carbon of aggregate fractions in the irrigated desert soil based on the long-term fertilizer experiment of China. Catena 173: 312-320. https://doi.org/10.1016/j.catena.2018.10.026
» https://doi.org/10.1016/j.catena.2018.10.026 -
Cui Z, Zhang Y, Chen X, Zhang C, Ma W, Huang C, et al. 2018. Pursuing sustainable productivity with millions of smallholder farmers. Nature 555: 363-366. https://doi.org/10.1038/nature25785
» https://doi.org/10.1038/nature25785 -
Dai X, Zhou W, Liu G, Liang G, He P, Liu Z. 2019. Soil C/N and pH together as a comprehensive indicator for evaluating the effects of organic substitution management in subtropical paddy fields after application of high-quality amendments. Geoderma 337: 1116-1125. https://doi.org/10.1016/j.geoderma.2018.11.023
» https://doi.org/10.1016/j.geoderma.2018.11.023 -
Debnath B , Irshad M , Mitra S , Li M , Rizwan HM , Liu S , et al . 2018. Acid rain deposition modulates photosynthesis, enzymatic and non-enzymatic antioxidant activities in tomato. International Journal of Environmental Research 12: 203-214. https://doi.org/10.1007/s41742-018-0084-0
» https://doi.org/10.1007/s41742-018-0084-0 -
Du Q, Yang J, Sadiq SSM, Yang R, Yu J, Li W. 2021. Comparative transcriptome analysis of different nitrogen responses in low-nitrogen sensitive and tolerant maize genotypes. Journal of Integrative Agriculture 20: 2043-2055. https://doi.org/10.1016/S2095-3119 (20)63220-8
» https://doi.org/10.1016/S2095-3119 (20)63220-8 -
Fanin N, Gundale MJ, Farrell M, Ciobanu M, Baldock JA, Nilsson MC, et al. 2018. Consistent effects of biodiversity loss on multifunctionality across contrasting ecosystems. Nature Ecology & Evolution 2: 269-278. https://doi.org/10.1038/s41559-017-0415-0
» https://doi.org/10.1038/s41559-017-0415-0 -
Grandy AS, Daly AB, Bowles TM, Gaudin ACM, Jiling A, Leptin A, et al. 2022. The nitrogen gap in soil health concepts and fertility measurement. Soil Biology and Biochemistry 175: 108856 https://doi.org/10.1016/j.soilbio.2022.108856
» https://doi.org/10.1016/j.soilbio.2022.108856 -
Hou P, Liu Y, Liu W, Yang H, Xie R, Wang K, et al. 2021. Quantifying maize grain yield losses caused by climate change based on extensive field data across China. Resources, Conservation & Recycling 174: 105811. https://doi.org/10.1016/j.resconrec.2021.105811
» https://doi.org/10.1016/j.resconrec.2021.105811 -
Jankowski K , Neill C , Davidson EA , Macedo MN , Costa Jr C , Galford GL , et al . 2018. Deep soils modify environmental consequences of increased nitrogen fertilizer use in intensifying Amazon agriculture. Scientific Reports 8: 13478. https://doi.org/10.1038/s41598-018-31175-1
» https://doi.org/10.1038/s41598-018-31175-1 -
Jiang C, Lu D, Zu C, Zhou J, Wang H. 2018. Root-zone fertilization improves crop yields and minimizes nitrogen loss in summer maize in China. Scientific Reports 8: 15139. https://doi.org/10.1038/s41598-018-33591-9
» https://doi.org/10.1038/s41598-018-33591-9 -
Jiao Y , Zhang W , Kim JYR , Deventer MJ , Vollering J , Rhew RC . 2022. Application of copper (II)-based chemicals induces CH 3 Br and CH 3 Cl emissions from soil and seawater. Nature Communications 13: 47. https://doi.org/10.1038/s41467-021-27779-3
» https://doi.org/10.1038/s41467-021-27779-3 -
Ke J , He R , Hou P , Ding C , Ding Y , Wang S , et al . 2018. Combined controlled-released nitrogen fertilizers and deep placement effects of N leaching, rice yield and N recovery in machine-transplanted rice. Agriculture, Ecosystems & Environment 265: 402-412. https://doi.org/10.1016/j.agee.2018.06.023
» https://doi.org/10.1016/j.agee.2018.06.023 -
Liu C , Liu Y , Gao W , Gao K , Li J , Sun B , et al . 2022. The effect of chamber placement site on N 2 O emission under different fertilizer regimes from maize field. Agriculture, Ecosystems and Environment 341: 108210. https://doi.org/10.1016/j.agee.2022.108210
» https://doi.org/10.1016/j.agee.2022.108210 -
Liu J, Liu M, Wu M, Jiang C, Chen X, Cai Z, et al. 2018. Soil pH rather than nutrients drive changes in microbial community following long-term fertilization in acidic Ultisols of southern China. Journal of Soils and Sediments 18: 1853-1864. https://doi.org/10.1007/s11368-018-1934-2
» https://doi.org/10.1007/s11368-018-1934-2 -
Liu P, Zhou D, Guo X, Yu Q, Zhang Y, Li H, et al. 2021. Response of water use and yield of dryland winter wheat to nitrogen application under different rainfall patterns. Scientia Agricultura Sinica 54: 3065-3076 (in Chinese, with abstract in English). https://doi.org/10.3864/j.issn.0578-1752.2021.14.012
» https://doi.org/10.3864/j.issn.0578-1752.2021.14.012 -
Liu Z, Gao F, Liu Y, Yang J, Zhen X, Li X, et al. 2019. Timing and splitting of nitrogen fertilizer supply to increase crop yield and efficiency of nitrogen utilization in a wheat-peanut relay intercropping system in China. The Crop Journal 7: 101-112. https://doi.org/10.1016/j.cj.2018.08.006
» https://doi.org/10.1016/j.cj.2018.08.006 -
Lu D , Song H , Jiang S , Chen X , Wang H , Zhou J . 2019. Managing fertilizer placement locations and source types to improve rice yield and the use efficiency of nitrogen and phosphorus. Field Crops Research 231: 10-17. https://doi.org/10.1016/j.fcr.2018.11.004
» https://doi.org/10.1016/j.fcr.2018.11.004 -
Megdal SB . 2018. Invisible water: the importance of good groundwater governance and management. npj Clean Water 1: 15. https://doi.org/10.1038/s41545-018-0015-9
» https://doi.org/10.1038/s41545-018-0015-9 -
Mi G, Wu D, Chen Y, Xia T, Feng G, Li Q, et al. 2018a. The ways to reduce chemical fertilizer input and increase fertilizer use efficiency in maize in northeast China. Scientia Agricultura Sinica 51: 2758-2770 (in Chinese, with abstract in English). https://doi.org/10.3864/j.issn.0578-1752.2018.14.013
» https://doi.org/10.3864/j.issn.0578-1752.2018.14.013 -
Mi W, Sun Y, Xia S, Zhao H, Mi W, Brookes PC, et al. 2018b. Effect of inorganic fertilizers with organic amendments on soil chemical properties and rice yield in a low-productivity paddy soil. Geoderma 320: 23-29. https://doi.org/10.1016/j.geoderma.2018.01.016
» https://doi.org/10.1016/j.geoderma.2018.01.016 -
Nguyen LTT, Osanai Y, Lai K, Anderson IC, Bange MP, Tissue DT, et al. 2018. Responses of the soil microbial community to nitrogen fertilizer regimes and historical exposure to extreme weather events: Flooding or prolonged-drought. Soil Biology and Biochemistry 118: 227-236. https://doi.org/10.1016/j.soilbio.2017.12.016
» https://doi.org/10.1016/j.soilbio.2017.12.016 -
Saïdou A, Balogoun I, Ahoton EL, Igué AM, Youl S, Ezui G, et al. 2018. Fertilizer recommendations for maize production in the South Sudan and Sudano-Guinean zones of Benin. Nutrient Cycling in Agroecosystems 110: 361-373. https://doi.org/10.1007/s10705-017-9902-6
» https://doi.org/10.1007/s10705-017-9902-6 -
Schjoerring JK, Cakmak I, White PJ. 2019. Plant nutrition and soil fertility: synergies for acquiring global green growth and sustainable development. Plant and Soil 434: 1-6. https://doi.org/10.1007/s11104-018-03898-7
» https://doi.org/10.1007/s11104-018-03898-7 -
Sileshi GW, Jama B, Vanlauwe B, Negassa W, Harawa R, Kiwia A, et al. 2019. Nutrient use efficiency and crop yield response to the combined application of cattle manure and inorganic fertilizer in sub-Saharan Africa. Nutrient Cycling in Agroecosystems 113: 181-199. https://doi.org/10.1007/s10705-019-09974-3
» https://doi.org/10.1007/s10705-019-09974-3 -
The State Council Information Office of the People's Republic of China [SCIO]. 2019. White Paper on "Food Security in China". Available at: http://www.scio.gov.cn/zfbps/32832/Document/1666192/1666192.htm [Accessed Oct 14, 2019] (in Chinese).
» http://www.scio.gov.cn/zfbps/32832/Document/1666192/1666192.htm -
Waqas MA , Li Y , Lal R , Wang X , Shi S , Zhu Y , et al . 2020. When does nutrient management sequester more carbon in soils and produce high and stable grain yields in China? Land Degradation & Development 31: 1926-1941. https://doi.org/10.1002/ldr.3567
» https://doi.org/10.1002/ldr.3567 -
Yang C, Liu N, Zhang Y. 2019. Soil aggregates regulate the impact of soil bacterial and fungal communities on soil respiration. Geoderma 337: 444-452. https://doi.org/10.1016/j.geoderma.2018.10.002
» https://doi.org/10.1016/j.geoderma.2018.10.002 -
Yang L, Yi S, Mao X, Tao G. 2018. Innovation design of fertilizing mechanism of seeder based on TRIZ Theory. IFAC-PapersOnLine 51: 141-145. https://doi.org/10.1016/j.ifacol.2018.08.077
» https://doi.org/10.1016/j.ifacol.2018.08.077 -
Yang Y , Liu H , Lv J . 2022. Evaluation of the applicability of organic amendments from microbially driven carbon and nitrogen transformations. Science of The Total Environment 817: 153020. https://doi.org/10.1016/j.scitotenv.2022.153020
» https://doi.org/10.1016/j.scitotenv.2022.153020 -
Yong T, Chen P, Dong Q, Du Q, Yang F, Wang X, et al. 2018. Optimized nitrogen application methods to improve nitrogen use efficiency and nodule nitrogen fixation in a maize-soybean relay intercropping system. Journal of Integrative Agriculture 17: 664-676. https://doi.org/10.1016/S2095-3119 (17)61836-7
» https://doi.org/10.1016/S2095-3119 (17)61836-7 -
Yu M, Meng J, Yu L, Su W, Afzal M, Li Y, et al. 2019. Changes in nitrogen related functional genes along soil pH, C, and nutrient gradients in the charosphere. Science of the Total Environment 650: 626-632. https://doi.org/10.1016/j.scitotenv.2018.08.372
» https://doi.org/10.1016/j.scitotenv.2018.08.372 -
Zhang F, Wei Y, Bo Q, Tang A, Song Q, Li S, et al. 2022. Long-term film mulching with manure amendment increases crop yield and water productivity but decreases the soil carbon and nitrogen sequestration potential in semiarid farmland. Agricultural Water Management 273: 107909. https://doi.org/10.1016/j.agwat.2022.107909
» https://doi.org/10.1016/j.agwat.2022.107909 -
Zhang J, Balkovic J, Azevedo LB, Skalský R, Bouwman AF, Xu G, et al. 2018. Analyzing and modelling the effect of long-term fertilizer management on crop yield and soil organic carbon in China. Science of The Total Environment 627: 361-372. https://doi.org/10.1016/j.scitotenv.2018.01.090
» https://doi.org/10.1016/j.scitotenv.2018.01.090 -
Zhang J, Bei S, Li B, Zhang J, Christie P, Li X. 2019a. Organic fertilizer, but not heavy liming enhances banana biomass, increases soil organic carbon and modifies soil microbiota. Applied Soil Ecology 136: 67-79. https://doi.org/10.1016/j.apsoil.2018.12.017
» https://doi.org/10.1016/j.apsoil.2018.12.017 -
Zhang X, Gao H, Peng C, Li Q, Zhu P, Gao Q. 2019b. Variation trend of soil organic carbon, total nitrogen and the stability of maize yield in black soil under long-term organic fertilization. Journal of Plant Nutrition and Fertilizers 25: 1473-1481 (in Chinese, with abstract in English). https://doi.org/10.11674/zwyf.2023015
» https://doi.org/10.11674/zwyf.2023015 -
Zhang W, Liang Z, He X, Wang X, Shi X, Zou C, et al. 2019c. The effects of controlled release urea on maize productivity and reactive nitrogen losses: A meta-analysis. Environmental Pollution 246: 559-565. https://doi.org/10.1016/j.envpol.2018.12.059
» https://doi.org/10.1016/j.envpol.2018.12.059
Edited by
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Edited by: Flávio Henrique Silveira Rabêlo
Publication Dates
-
Publication in this collection
13 May 2024 -
Date of issue
2024
History
-
Received
12 Apr 2023 -
Accepted
26 June 2023