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Revista do Instituto de Medicina Tropical de São Paulo

On-line version ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.36 no.5 São Paulo Sept./Oct. 1994

https://doi.org/10.1590/S0036-46651994000500001 

SEROLOGY

 

Gelatin particle indirect agglutination test for serodiagnosis of schistosomiasis: comparative study with enzyme-linked immunosorbent assay

 

Teste de aglutinação indireta com partículas de gelatina: estudo comparativo com enzima imunoensaio

 

 

Jun KobayashiI,IV; Flair José CarrilhoII; Tetsu ShimabukuroI; Luiz Caetano da SilvaIII; Elza Cotrim SoaresI; Nancy Fusae NishimuraI; Pedro Paulo ChieffiIII,VI; Yoshiya SatoIV,V

ILaboratório de Imuno-Parasitologia, Gastrocentro, State University of Campinas, Campinas, SP, Brasil
IIDepartment of Gastroenterology, School of Medicine, University of São Paulo, SP, Brasil
IIIInstituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, SP, Brasil. (LIM-06)
IVDepartment of Parasitology, School of Medicine, University of the Ryukyus, Japan
VResearch Center of Comprehensive Medicine, School of Medicine, University of the Ryukyus, Japan
VIDepartment of Preventive Medicine, School of Medicine, University of São Paulo, São Paulo, SP, Brasil

Correspondence to

 

 


SUMMARY

A new serological test, the gelatin particle agglutination test (GPAT), was used for the serodiagnosis of schistosomiasis mansoni. This technique showed the sensitivity (90.6%) and specificity (97.8%) close to those of enzyme-linked immunosorbent assay. The GPAT can be easily and rapidly performed without specialized equipment, by using lyophilized antigen-coated gelatin particles. The test also seems to be useful for mass screening of Schistosoma infection in field conditions.

Keywords: Schistosoma mansoni; Serodiagnosis; GPAT (gelatin particle agglutination test); ELISA.


RESUMO

Um novo teste sorológico, o teste de aglutinação com partículas de gelatina (TAPG), foi utilizado para o diagnóstico sorológico de esquistossomose mansônica. Esta técnica mostra que a sensibilidade (90.6%) e especificidade (97.8%) chegam próximas do teste de enzima imunoensaio.
O TAPG pode ser fácil e rapidamente realizado sem equipamentos especializados usando partículas liofilizadas sensibilizadas com o antígeno.
O teste mostrou ser também útil em condições de campo para triagem em massa da infecção pelo Schistosoma mansoni.


 


Full text available only in PDF format.

Texto completo disponível apenas em PDF.

 


ACKNOWLEDGMENT

We are grateful to Fujirebio Inc., Tokyo, Japan (Mr. S. Hanzawa and Mr. Y. Nagafuchi) for supplying the gelatin particles and to Prof. Dr. M. Tanabe, Keio University, for his kind advices. This study was supported in part by The JICA (Japan International Cooperation Agency) Technical Co-operation for the Project on Gastroenterological Diagnosis and Research Center of the State University of Campinas.

 

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Correspondence to:
Jun Kobayashi
Gastrocentro Universidade Estadual de Campinas, Cidade Universitária "Zeferino Vaz"
B. Geraldo, 13081 -970 Campinas, SP, Brasil

Recebido para publicação em 02/12/1993
Aceito para publicação em 12/04/1994

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