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Revista do Instituto de Medicina Tropical de São Paulo

versão On-line ISSN 1678-9946

Rev. Inst. Med. trop. S. Paulo vol.61  São Paulo  2019  Epub 20-Dez-2018 

Brief Communication

Bloodstream infection in hematopoietic stem cell transplantation outpatients: risk factors for hospitalization and death

Rachel Russo1 

Elisa Teixeira Mendes1  2

Anna Sara Levin1  3 

Frederico Dulley4 

Maura S. Oliveira1 

Maria Aparecida Shikanai-Yasuda1 

Silvia Figueiredo Costa1  3

1Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil

2Pontifícia Universidade Católica de Campinas, Faculdade de Medicina, Programa de Pós-Graduação em Ciências da Saúde, Campinas. São Paulo, Brazil

3Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, Laboratório de Bacteriologia (LIM-54), São Paulo, São Paulo, Brazil

4Universidade de São Paulo, Faculdade de Medicina, Disciplina de Hematologia, São Paulo, São Paulo, Brazil


We described 235 bloodstream infection (BSI) episodes in 146 hematopoietic stem cell transplantation (HSCT) outpatients and evaluated risk factors for hospitalization and death. Records of outpatients presenting with positive blood cultures over a 5-year period (January 2005 to December 2008) were reviewed. Variables with p< 0.1 in bivariate analysis were used in a regression logistic model. A total of 266 agents were identified, being 175 (66.7%) gram-negative, 80 (30.3%) gram-positive bacteria and 9 (3.4%) fungi. The most common underlying disease was acute leukemia 40 (27.4%), followed by lymphoma non-Hodgkin 26 (18%) and 87 patients (59.6%) were submitted to allogeneic hematopoietic stem cell transplant (HSCT). BSI episodes were more frequent during the first 100 days after transplantation (183 or 77.8%), and ninety-one (38.7%) episodes of BSI occurred up to the first 30 days. Hospitalization occurred in 26% of the episodes and death in 10% of cases. Only autologous HSCT was protector for hospitalization. Although, central venous catheter (CVC) withdrawal and the Multinational Association of Supportive Care in Cancer (MASCC) score up to 21 points were protector factors for death in the bivariate analysis, only MASCC remained as protector.

KEYWORDS: Bacteremia; Hematopoietic stem cell transplantation; Outpatients; Outcome; Resistance


Bloodstream infection (BSI) is the most relevant infections in patients undergoing hematopoietic stem cell transplant (HSCT) with high morbidity and mortality1. Until recently, gram-positive bacteria (GPB) were the most common agents causing BSI in these patients; moreover, in the last years there has been an increase of gramnegative bacteria (GNB) in several hospitals2.

Despite the increase in the number of allogeneic (allo) HSCT patients with early discharge and outpatient autologous (auto) transplantation programs; there is a lack of studies evaluating the safety of treating infections in HSCT outpatients.

The objective of this study was to evaluate the etiological agents causing BSI in HSCT outpatients as well as the risk factors associated with hospitalization and 30-day mortality.


The Hospital das Clinicas is a teaching hospital with 2,200-beds and is a reference center for auto and allo-HSCT in Brazil. The outpatient unit is open from 7 am to 7 pm, every day of the week, including weekends. All patients submitted to HSCT were hospitalized at the time of transplantation.

Records of outpatients presenting with positive blood cultures over a 5-year period (January 2005 to December 2008) were reviewed. This study was approved by the Ethics Committee of the Hospital das Clinicas of University of Sao Paulo.

The BSI definition used were patients with positive blood culture collected through the central venous catheter (CVC) or a peripheral access during a period in which patients had fever and physicians had described antibiotics. The end points evaluated were hospitalization and 30-day mortality BSI onset. Clinical and demographic variables at the time of BSI, such as gender, age, underlying disease, length of hospitalization, type of HSCT, CVC (type and withdrawal), the presence of mucositis or graft-versus-host disease (GVHD) and Multinational Association of Supportive Care in Cancer (MASCC) risk index score were evaluated. Hospitalizations depended on the clinical status, and when possible, the treatment was administered in the outpatient unit in which blood cultures were indicated in the presence of fever, hemodynamic instability or any infectious symptoms.

Microorganism identification and antimicrobial susceptibility test was performed using Vitek® (laboratory BioMérieux) and disk diffusion test followed the updated recommendations of CLSI (Clinical and Laboratory Standards Institute).

Data were analyzed using the softwares Epi Info version 3.5.1 and STATA. The two-tailed Fisher's exact test was used for categorical variables and the Wilcoxon test was used for continuous variables. Logistic regression models were developed to identify factors associated with hospitalization within 21 days onset of BSI and 30-day mortality onset BSI. All non-overlapping variables with p < 0.1 on bivariate analysis were entered into a stepwise forward model. A p-value <0.05 was considered statistically significant.


A total of 743 HSCT outpatients were evaluated during the study period and the records of 146 of them were analyzed. A total of 235 BSI episodes were identified among the 146 patients evaluated, being 207 (88%) monomicrobial and 28 (12%) polymicrobial. The hospitalization occurred in 61 (26%) episodes, the more frequent reason was septic shock, 18/61 (31%) (Table 1). A total of 266 agents were isolated, being 175 (66.7%) gram-negative bacteria (GNB), 80 (30.3%) gram-positive bacteria (GPB) and 9 (3.4%) fungi (Table 1).

Table 1 Clinical characteristic of 235 bloodstream infection episodes in 146 HSCT outpatients in Hospital das Clinicas, Brazil 

Characteristic N (%)
Mucositis 63 (25)
Grade of mucositis (%)
I 25 (48)
II 15 (29)
III 10(19)
IV 2 (4)
GVHD (%)
Skin 58 (70)
Gut 48 (60)
Lung 5 (6)
Grade of GVHD (%)
I 12 (44)
II 4 (15)
III 8 (30)
IV 3 (11)
Severe neutropenia (<100 cells/mm3) (%) 63 (28)
ANC, median (range) (cells/mm3) 2,300(0-37,600)
Length of neutropenia, median (range) (days) 6 (1-30)
Imune recovery after transplant (%)
Until 30 days after BMT 91 (39)
30 days to 100 days after BMT 92 (39)
After 30 days of BMT 52 (22)
CVC (%)
Non-tunneled 2 (1)
Tunneled 191 (84)
Totally implantable 9 (4)
Absence 25 (11)
CVC infection (%) 19 (9)
CVC withdrawal (%) 66 (33)
Previous usage of antimicrobials (%) 190 (81)
Until 30 days before collection (%) 187 (80)
On time of collection (%) 139 (60)
Cefepime 113 (58)
Levofloxacin 52 (27)
Meropenem 105 (54)
Teicoplanin 136 (70)
BSI agents (%)
Most frequent Gram-positive bacteria 80 (30)
Staphylococcus coagulase negative 31 (12%)
Enterococcus faecium 9 (3%)
Micrococcus spp 9 (3%)
Staphylococcus aureus 7 (3%)
Streptococcus pneumoniae 4 (1%)
Most frequent Gram-negative bacteria 175 (67)
Stenotrophomonas maltophilia 39 (15%)
Acinetobacter spp 24 (9%)
Pseudomonas aeruginosa 15 (6%)
Burkholderia cepacia 13 (5%)
Klebsiella spp 12 (4%)
Enterobacter cloacae 11 (4%)
Serratia marcescens 9 (3%)
Agrobacterium spp 8 (3%)
Fungi 9 (3)
Candida spp 6 (2%)
Cultures (%)
Monomicrobial 207 (88)
Polymicrobial 28 (12)
Hospitalization up to 21 days after culture (%) 61 (26)
Hospitalization reasons (%)
infection complications 8 (14)
Sepsis 12 (14)
Sepsis shock 18 (31)
Others 20
New infection (%) 84 (36)
Length of positive culture until hospitalization, media (range) (days) 3.3 (0-21)
MASCC, median (range) 22 (8-26)
Death up to 30 days (%) 22 (10)
Time between positive culture and death, median (range) (days) 42 (0-995)

CVC: central venous catheter; MASCC: Multinational Association for Supportive Care in Cancer Risk Index, GVHD: Graft-Versus-Host Disease. Others BSI agents: Leuconostoc spp, Bacillus spp, Sphingomonas paucimobilis, Chryseobacterium spp, E.coli, Leuconostoc spp, Aeromonas spp. Other: myelodysplastic syndrome (25%), Fanconi anemia (25%), myelofibrosis (12.5%), Ewing’ sarcoma (6.25%), Krabbe disease (6.25%), germinal cells tumor (6.25%), adrenoleukodystrophy (6.25%), seminoma (6.25%).

Resistance to cefepime in P. aeruginosa was identified in only 2/15 (13%) of the isolated agents, to imipenem in 2/14 (14%), and to meropenem in 3/10 (27%). Among Enterobacter cloacae, resistance to cefepime occurred in 3/11 (27%) cases, no carbapenem resistance was identified. Among Acinetobacter spp, resistance to cefepime occurred in 3/24 (14%) and resistance to imipenem in 2/24 (8%). Resistance to levofloxacin occurred in 1/39 (3%) and to trimethoprim-sulfamethoxazole in 3/39 (8%) of S. maltophilia isolates. Resistance to vancomycin was identified in 7/9 (78%) of E. faecium BSI. Gram-negative bacteria accounted for 72% of bloodstream infections in allogeneic transplants and 62% of autologous ones (p=0.12). The mean time between transplantation and BSI was higher among allogeneic than autologous HSCT patients (145.5 and 44.1 days, respectively), probably because allogeneic patients remain hospitalized for longer periods after transplantation and at higher risk of infection after discharge. Death in 30 days were 8.5% in autologous and 15.2% in allogenic HSCT (p=0.17). Median time between positive culture and death in days were 56 days in gram-negative and 27 days in gram-positive infections.

The bivariate analysis showed that previous use of levofloxacin, MASCC score higher than 21 and autologous (auto)-HSCT were protector factors and neutropenia was a risk factor for hospitalization. Moreover, only auto-HSCT remained as protector in the multivariate analysis (Table 2). The bivariate analysis showed that the CVC withdrawal, and MASCC higher than 21 points were protector factors for death (Table 2). Moreover, only MASCC score remained as protector (Table 2).

Table 2 Risk factors for hospitalization and death in 30 days among 235 episodes of BSI in HSCT outpatients patients. 

Variables Hospitalization N (%) Bivariate Analysis RR (CI 95%) P-value Multivariate analysis OR (CI 95%) p-value
No Yes
Previous usage of levofloxacin (%) 44 (85) 8 (16) 0.82 (0.70-0.97) 0.030 0.56 (0.23-1.40) 0.22
Autologous BMT (%) 74 (88) 10 (12) 0.75 (0.65-0.86) 0.0001 0.23 (0.11-0.68) 0.005
Bacteremia due to gram-positive* (%) 46 (66) 24 (34) 1.17 (0.97-1.41) 0.050 1.16 (0.51-2.65) 0.71
Mucositis (%) 43 (68) 20 (32) 1.11 (0.91-1.33) 0.16
Severe neutropenia (%) 38 (60) 25 (40) 1.28 (1.02-1.60) 0.007 1.67 (0.71-3.92) 0.24
CVC withdrawn (%) 52 (79) 14 (21) 0.91 (0.77-1.07) 0.19
Length of BMT until bacteremia, median (range) (days) 174 61 60 (6-746) 0.21
ANC. median (range) cels/mm3 164 61 220 (0-23,400) 0.12
MASCC. median 174 59 18 (8-26) < 0.0001
MASCC score higher than 21 (%) 101 (82) 23 (18) 0.82 (0.70-0.96) 0.008 0.55 (0.27-1.13) 0.10
Death N (%) Bivariate Analysis RR (CI 95%) P-value Multivariate analysis OR (CI 95%) p-value
Yes No
Age. (median) 32 38 0.29
Previous usage of levofloxacin (%) 26 (93) 2 (7) 0.89 (0.77-1.03) 0.17
Autologous BMT (%) 54 (92) 5 (8) 0.93 (0.86-1.01) 0.17 3.73 (0.90-15.28) 0.06
Bacteremia Gram-positive* (%) 39 (81) 9(19) 1.11 (0.95-1.29) 0.10
Bacteremia Gram-negative (%) 81(90) 19 (9) 0.91 (0.79-1.0) 0.15
Fungi 4 (100) 0 0.87 (0.81-0.92) 0.58
Mucositis (%) 38 (83) 8 (17) 1.02 (0.92-1.12) 0.41
GVHD (%) 34 (83) 7(17) 1.07 (0.92-1.25) 0.21
Severe neutropenia (%) 32 (82) 7 (18) 1.06 (0.90-1.23) 0.28
CVC withdrawal (%) 34 (97) 1 (3) 0.87 (0.79-0.97) 0.050 40 (0.07-2.38) 0.32
ANC, median (range) (cels/mm3) 2,350 1,250 0.54
Duration of neutropenia. (days, mean) 3.4 4.5 0.43
MASCC. median 21 16 0.0001 0.78 (0.68-0.90) 0.001
MASCC score higher than 21 (%) ** 127 (88) 18 (12) 0.90 (0.83-1.02) 0.07
Shock 11 (27) 29 (73) 1.70 (0.43-6.69) 0.34

CVC: central venous catheter; MASCC: Multinational Association for Supportive Care in Cancer Risk Index. GVHD: Graft-Versus-Host Disease


We observed a high proportion of GNB-BSI in our HSCT outpatient unit. The predominance of GNB has been reported by Brazilian studies that evaluated HSCT inpatients3. In our casuistic, BSI episodes occurred mainly in the first 100 days after the transplantation, similarly to other studies in the literature1,2 and S. maltophilia was the most frequent agent in both monomicrobial and polymicrobial BSI. In general, this agent causes outbreaks in BMT settings4. Moreover, as in our hospital, a recent study has shown that S. maltophilia and P. aeruginosa were the most commonly isolated GNB in HSCT patients5. We observed a high frequency of infection caused by waterborne bacteria (18%). Infection by these agents is rare even in neutropenic patients6. However, our service recommends the protection of the CVC's lumen with a plastic dressing to prevent exposure to tap water, a potential source of waterborne agents but it was not possible to assess patients’ compliance with this recommendation. Thus, inappropriate care of CVC at home during baths might explain this finding7.

In our study the 30-day mortality onset BSI was 10% and hospitalization occurred in 26% of patients. Moreover, the multivariate analysis showed that autologous HSCT was the only independent protector factor for hospitalization. MASCC score and CVC withdrawal were protective factors for death in the bivariate analysis. In addition, only MASCC score higher than 21, remained as protector factor in the multivariate analysis. Unfortunately, in our study the catheter removal was not carried out as recommended in the literature because of the difficulty of venous access.

In the literature the presence of infection is a major cause of death in HSCT patients1. Ortega et al showed that 10% of HSCT inpatients with infection evolved to death6. Therefore, the proportion of deaths in the present study in outpatients was similar to that described in BSI HSCT inpatients8. Vancomycin-resistant enterococcus (VRE) mortality in HSCT patients ranges from 7% to 34%9 similar to our findings. In contrast, authors have reported a high mortality of BSI caused by carbapenem-resistant GNB in HSCT10.

In conclusion, GNB were the most frequent agents causing BSI in HSCT outpatients in our hospital; hospitalization rate was low and auto-HSCT was a protector factor for hospitalization and the MASCC score higher than 21 points for 30-day mortality.


No funding of any kind has been received.


1. Schuster MG, Cleveland AA, Dubberke ER, Kauffman CA, Avery RK, Husain S, et al. Infections in hematopoietic cell transplant recipients: results From the Organ Transplant Infection Project, a multicenter, prospective, cohort study. Open Forum Infect Dis. 2017;4:ofx050. [ Links ]

2. Averbuch D, Tridello G, Hoek J, Mikulska M, Akan H, Yanez San Segundo L, et al. Antimicrobial resistance in Gram-negative rods causing bacteremia in hematopoietic stem cell transplant patients: intercontinental prospective study of Infectious Diseases Working Party of the European Bone Marrow Transplantation group. Clin Infect Dis. 2017;65:1819-28. [ Links ]

3. Mendes ET, Dulley F, Basso M, Batista MV, Coracin F, Guimarães T, et al. Healthcare-associated infection in hematopoietic stem cell transplantation patients: risk factors and impact on outcome. Int J Infect Dis. 2012;16:e424-8. [ Links ]

4. Labarca JA, Leber AL, Kern VL, Territo MC, Brankovic LE, Bruckner DA, et al. Outbreak of Stenotrophomonas maltophilia bacteremia in allogenic bone marrow transplant patients: role of severe neutropenia and mucositis. Clin Infect Dis. 2000;30:195-7. [ Links ]

5. Girmenia C, Bertaina A, Piciocchi A, Perruccio K, Algarotti A, Busca A, et al. Incidence, risk factors and outcome of pre-engraftment gram-negative bacteremia after allogeneic and autologous hematopoietic stem cell transplantation: an Italian prospective multicenter survey. Clin Infect Dis. 2017;65:1884-96. [ Links ]

6. Ortega M, Rovira M, Almela M, Marco F, de la Bellacasa JP, Martínez JA, et al. Bacterial and fungal bloodstream isolates from 796 hematopoietic stem cell transplant recipients between 1991 and 2000. Ann Hematol. 2005;84:40-6. [ Links ]

7. Toscano CM, Bell M, Zukerman C, Shelton W, Novicki TJ, Nichols WG, et al. Gram-negative bloodstream infections in hematopoietic stem cell transplant patients: the roles of needleless device use, bathing practices, and catheter care. Am J Infect Control. 2009;37:327-34. [ Links ]

8. McDiarmid S, Hutton B, Atkins H, Bence-Bruckler I, Bredeson C, Sabri E, et al. Performing allogeneic and autologous hematopoietic SCT in the outpatient setting: effects on infectious complications and early transplant outcomes. Bone Marrow Transplant. 2010;45:1220-6. [ Links ]

9. Tavadze M, Rybicki L, Mossad S, Avery R, Yurch M, Pohlman B, et al. Risk factors for vancomycin-resistant enterococcus bacteremia and its influence on survival after allogeneic hematopoietic cell transplantation. Bone Marrow Transplant. 2014;10:1310-6. [ Links ]

10. Forcina A, Baldan R, Marasco V, Cichero P, Bondanza A, Noviello M, et al. Control of infectious mortality due to carbapenemase-producing Klebsiella pneumoniae in hematopoietic stem cell transplantation. Bone Marrow Transplant. 2017;52:114-9. [ Links ]

Received: August 30, 2018; Accepted: November 26, 2018

Correspondence to: Silvia Figueiredo Costa Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, Laboratório de Bacteriologia (LIM-54), Av. Dr. Enéas de Carvalho Aguiar, 470, Cerqueira Cesar, CEP 05403-000, São Paulo, SP, Brazil Tel: + 55 11 3066-7030, Fax: + 55 11,


None to declare.

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