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au:COELHO, ANA MARIA MENDONCA

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Changes in body composition of burn patients during the phases of response to trauma
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Arruda, Andressa Maranhão de

; Gadelha, Patrícia Calado Ferreira Pinheiro

; Soares, Bruna Lúcia de Mendonça

; Dowsley, Catiana Coelho Cabral

; Lemos, Maria da Conceição Chaves de

; Santos, Ana Célia Oliveira dos

Revista Brasileira de Enfermagem

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2023, Volume 76 N. 6 elocation e20230039

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: To assess the changes in body composition of burn patients through electrical bioimpedance in the phases of response to trauma. Methods: a longitudinal observational study, carried out from October 2019 to March 2020. Sociodemographic, clinical, epidemiological, anthropometric and body composition data were collected. Statistical analysis was performed with SPSS, considering a significance of 5%. The comparison between variables was performed using the paired Student’s t test. Results: the sample consisted of 58 adult burn patients, with a mean age of 38.2±12.5 years. The mean body surface area (BSA) with burns was 10.8±7.3%. Nutritional assessment demonstrated a depletion of body weight, Body Mass Index, fat-free mass and muscle mass in the phases of response to trauma (p<0.005). Conclusion: metabolic alterations in the different phases of the metabolic response to trauma led to a depletion of the nutritional status of burn patients of both sexes during hospitalization.

RESUMEN Objetivo: evaluar alteraciones en la composición corporal de pacientes quemados mediante bioimpedancia eléctrica en las fases de respuesta al trauma. Métodos: estudio observacional longitudinal, realizado de octubre de 2019 a marzo de 2020. Se recogieron datos sociodemográficos, clínicos, epidemiológicos, antropométricos y de composición corporal. El análisis estadístico se realizó mediante SPSS, considerando una significancia del 5%. La comparación entre variables se realizó mediante la prueba de la t de Student pareada. Resultados: la muestra estuvo compuesta por 58 pacientes adultos quemados, con una edad media de 38,2±12,5 años. El área de superficie corporal media (ASC) con quemaduras fue de 10,8±7,3%. La evaluación nutricional mostró disminución del peso corporal, índice de masa corporal, masa magra y masa muscular en las fases de respuesta al trauma (p<0,005). Conclusión: los cambios metabólicos en las diferentes fases de la respuesta metabólica al trauma llevaron a la disminución del estado nutricional de los pacientes quemados de ambos sexos durante la hospitalización.

RESUMO Objetivo: avaliar as alterações na composição corporal de pacientes queimados por meio da bioimpedância elétrica nas fases de resposta ao trauma. Métodos: estudo observacional longitudinal, realizado de outubro de 2019 a março de 2020. Foram coletados dados sociodemográficos, clínicos, epidemiológicos, antropométricos e de composição corporal. A análise estatística foi realizada com o SPSS, considerando significância de 5%. Comparação entre as variáveis foi realizada por meio do teste t de Student pareado. Resultados: a amostra foi composta por 58 pacientes adultos queimados, com média de 38,2±12,5 anos. A média da área de superfície corporal (ASC) com queimaduras foi de 10,8±7,3%. A avaliação nutricional demonstrou depleção do peso corporal, índice de massa corporal, massa magra e massa muscular nas fases de resposta ao trauma (p<0,005). Conclusão: alterações metabólicas nas diferentes fases da resposta metabólica ao trauma levaram a depleção do estado nutricional de pacientes queimados de ambos os sexos durante a internação.

https://doi.org/10.1590/0034-7167-2023-0039

Contribution of public oral pathology services to the diagnosis of oral and oropharyngeal cancer in Brazil
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LOUREDO, Brendo Vinicius Rodrigues

; CURADO, Maria Paula

; PENAFORT, Paulo Victor Mendes

; DE ARRUDA, José Alcides Almeida

; ABREU, Lucas Guimarães

; MESQUITA, Ricardo Alves

; PINTO-JÚNIOR, Décio dos Santos

; ABRAHÃO, Aline Corrêa

; ANDRADE, Bruno Augusto Benevenuto de

; AGOSTINI, Michelle

; MORAES, Renata Mendonça

; ANBINDER, Ana Lia

; DOURADO, Pedro Henrique Silva

; SANTOS, Teresa Cristina Ribeiro Bartholomeu dos

; PIRES, Fábio Ramoa

; BORDIGNON, Natalia Cristina Trentin

; GONDAK, Rogério Oliveira

; DE OLIVEIRA, Marcia Gaiger

; CARRARD, Vinicius Coelho

; MARTINS, Manoela Domingues

; SOUSA-NETO, Sebastião Silvério

; ARANTES, Diego Antônio Costa

; MENDONÇA, Elismauro Francisco

; CIESLAK-SANCHES, Silvia Roberta

; ANTUNES, Daniella Moraes

; AMARAL-SILVA, Gleyson Kleber do

; MANIERI, Patricia Rubia

; RAMALHO, Luciana Maria Pedreira

; DOS SANTOS, Jean Nunes

; LEONEL, Augusto César Leal da Silva

; PEREZ, Danyel Elias da Cruz

; VERHEUL, Hannah Carmem Carlos Ribeiro Silva

; BARROSO, Keila Martha Amorim

; RODRIGUES, Flávia Luiza Santos

; GONZAGA, Amanda Katarinny Goes

; FERNANDES, Romana Renery

; DE SOUZA, Lélia Batista

; SOUZA, Lucas Lacerda de

; PONTES, Flávia Sirotheau Corrêa

; PONTES, Hélder Antônio Rebelo

; SILVA, Caroline Alfaia

; CÂMARA, Jeconias

; LIBÓRIO-KIMURA, Tatiana Nayara

; SANTOS-SILVA, Alan Roger

; LOPES, Márcio Ajudarte

; ALMEIDA, Oslei Paes de

; ROMAÑACH, Mário José

; VARGAS, Pablo Agustin

Brazilian Oral Research

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2023, Volume 37 elocation e126

Abstract: En | Text: En | PDF: En

Abstract This study aimed to evaluate the contribution of oral and maxillofacial pathology laboratories (OMPLs) in Brazilian public universities to the diagnosis of lip, oral cavity, and oropharyngeal squamous cell carcinoma (SCC). A cross-sectional study was performed using biopsy records from a consortium of sixteen public OMPLs from all regions of Brazil (North, Northeast, Central-West, Southeast, and South). Clinical and demographic data of patients diagnosed with lip, oral cavity, and oropharyngeal SCC between 2010 and 2019 were collected from the patients’ histopathological records. Of the 120,010 oral and maxillofacial biopsies (2010-2019), 6.9% (8,321 cases) were diagnosed as lip (0.8%, 951 cases), oral cavity (4.9%, 5,971 cases), and oropharyngeal (1.2%, 1,399 cases) SCCs. Most cases were from Brazil’s Southeast (64.5%), where six of the OMPLs analyzed are located. The predominant profile of patients with lip and oral cavity SCC was Caucasian men, with a mean age over 60 years, low schooling level, and a previous history of heavy tobacco consumption. In the oropharyngeal group, the majority were non-Caucasian men, with a mean age under 60 years, had a low education level, and were former/current tobacco and alcohol users. According to data from the Brazilian National Cancer Institute, approximately 9.9% of the total lip, oral cavity, and oropharyngeal SCCs reported over the last decade in Brazil may have been diagnosed at the OMPLs included in the current study. Therefore, this data confirms the contribution of public OMPLs with respect to the important diagnostic support they provide to the oral healthcare services extended by the Brazilian Public Health System.

https://doi.org/10.1590/1807-3107bor-2023.vol37.0126

Production and validation of the short film Pés que te quero®: educational technology for people with diabetes
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Menezes, Luciana Gomes Catunda

; Guedes, Maria Vilani Cavalcante

; Oliveira, Sherida Karinini Paz de

; Rocha, Roseanne Montargil

; Pinheiro, Ana Karina Bezerra

; Silva, Lúcia de Fátima da

; Moura, Denizielle de Jesus Moreira

; Coelho, Manuela de Mendonça Figueirêdo

Revista Brasileira de Enfermagem

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2022, Volume 75 N. 5 elocation e20210329

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: descrever a produção e validação de tecnologia educacional do tipo curta-metragem para prevenção de úlceras do pé diabético. Métodos: estudo metodológico, com foco na validação de filme curta-metragem. A construção do filme foi executada em três etapas: pré-produção, produção e pós-produção. Empreendeu-se a validação interna com 37 avaliadores divididos em dois grupos: 31 proficientes enfermeiros, seis da área de comunicação. A validação externa foi realizada por 15 pessoas com Diabetes Mellitus. Resultados: os proficientes da enfermagem validaram o roteiro com Índice de Validade de Conteúdo (IVC) total de 0,95, com confiabilidade interna de 0,849 conferido pelo alfa de Cronbach, proficientes da comunicação validaram com IVC de 0,97 e público-alvo com 0,95 (clareza) e 0,97 (relevância). Conclusão: o curta-metragem se configurou como tecnologia educativa válida e confiável para promover o autocuidado com os pés às pessoas com Diabetes Mellitus.

ABSTRACT Objective: To describe the production and validation of short film type educational technology for the prevention of diabetic foot ulcers. Methods: A methodological study focused on the validation of a short film. The construction of the film was carried out in three stages: pre-production, production, and post-production. Thirty-seven evaluators undertook the internal validation, divided into two groups of 31 proficient nurses and six from the communication area. Fifteen people with diabetes mellitus performed the external validation. Result: The nursing proficient validated the script with a total content validity index of 0.95, with internal reliability of 0.849 conferred by Cronbach’s Alpha. Communication specialists validated with a content validity index of 0.97; and target audience with 0.95 (clarity) and 0.97 (relevance). Conclusion: The study showed that the short film is a valid and reliable educational technology to promote foot care to people with diabetes mellitus.

RESUMEN Objetivo: Describir la producción y validación de tecnología educacional del tipo cortometraje para prevención de úlceras del pie diabético. Métodos: Estudio metodológico, con enfoque en la validación del cortometraje. La construcción de la película fue ejecutada en tres etapas: preproducción, producción y postproducción. Se emprendió la validación interna con 37 evaluadores divididos en dos grupos: 31 proficientes enfermeros y 6 del área de comunicación. La validación externa fue realizada por 15 personas con diabetes mellitus. Resultados: Los proficientes de la enfermería validaron el guión con Índice de Validez de Contenido total de 0,95, con confiabilidad interna de 0,849 conferido por el alfa de Cronbach; proficientes de la comunicación validaron con Índice de Validez de Contenido de 0,97; y público objeto, con 0,95 (claridad) y 0,97 (relevancia). Conclusión: El cortometraje se configuró como tecnología educativa válida y confiable para promover el autocuidado con los pies a las personas con diabetes mellitus.

https://doi.org/10.1590/0034-7167-2021-0329

Use of a therapeutic communication application in the Nursing undergraduate program: randomized clinical trial
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Coelho, Manuela de Mendonça Figueirêdo

; Miranda, Karla Corrêa Lima

; Melo, Regina Claúdia de Oliveira

; Gomes, Linicarla Fabiole de Souza

; Monteiro, Ana Ruth Macêdo

; Moreira, Thereza Maria Magalhães

Revista Latino-Americana de Enfermagem

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2021, Volume 29 elocation e3456

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Objetivo: analisar o efeito no conhecimento sobre a comunicação terapêutica pelos acadêmicos de Enfermagem com o uso de aplicativos. Método: ensaio clínico randomizado e controlado realizado junto a 60 estudantes de Enfermagem. No tópico Grupamentos e técnicas de comunicação terapêutica, o grupo intervenção-GI (n=30) utilizou o aplicativo e o grupo controle-GC (n=30) foi submetido à aula tradicional. Foi avaliado o conhecimento pré (Teste zero -0) e pós-teste (imediato-Teste 1 e após 30 dias-Teste 2) dos envolvidos. Resultados: o teste 1, realizado imediatamente após a intervenção, mostrou uma média de 11 acertos no grupo controle e 13 no intervenção, com significância estatística (p=0,036). O teste 2 mostrou uma diminuição dos acertos nos dois grupos (GI=10,87 e GC=9,3), mas manteve a diferença entre GI e GC no pós-teste (p<0,01). Conclusão: a utilização do aplicativo sobre a comunicação terapêutica favoreceu o conhecimento dos estudantes, quando comparado ao método tradicional de ensino. Registro REBEC RBR-4TF6MR.

Objective: to analyze the effect on the knowledge of therapeutic communication by Nursing students through the use of applications. Method: a randomized and controlled clinical trial conducted with 60 nursing students. In the topic Groups and therapeutic communication techniques, the intervention-IG group (n=30) used the application and the control-CG group (n=30) was submitted to the traditional class. The pre- (Zero Test -0) and post-test knowledge (immediate-Test 1 and after 30 day-Test 2) of those involved were evaluated. Results: Test 1, performed immediately after the intervention, showed a mean of 11 hits in the control group and 13 in the intervention, with statistical significance (p=0.036). Test 2 showed a decrease in hits in both groups (IG=10.87 and CG=9.3), but maintained the difference between IG and CG in the post-test (p<0.01). Conclusion: the use of the application on therapeutic communication favored the knowledge of the students, when compared to the traditional teaching method. REBEC RBR-4TF6MR Registration.

Objetivo: analizar el efecto del conocimiento sobre comunicación terapéutica por los académicos de enfermería con el uso de aplicativos. Método: ensayo clínico aleatorio controlado, realizado en 60 estudiantes de enfermería. En el tópico, agrupamientos y técnicas de comunicación terapéutica, el grupo intervención-GI (n=30) utilizó el aplicativo y el grupo control-GC (n=30) fue sometido a una clase tradicional. Fue evaluado el conocimiento pretest (Test cero -0) y postest (inmediato-Test 1 y después de 30 días-Test 2) de los participantes. Resultados: el test 1, realizado inmediatamente después de la intervención, mostró media de 11 aciertos en el grupo control y 13 en la intervención, con significación estadística de p=0,036. El test 2 mostró diminución de los aciertos en los dos grupos (GI=10,87 y GC=9,3), pero mantuvo diferencia entre GI y GC en el postest (p<0,01). Conclusión: la utilización del aplicativo sobre comunicación terapéutica favoreció el conocimiento de los estudiantes al ser comparado con el método tradicional de enseñanza. Registro REBEC RBR-4TF6MR.

https://doi.org/10.1590/1518-8345.4461.3456 718 downloads

Updated Cardiovascular Prevention Guideline of the Brazilian Society of Cardiology - 2019
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Précoma, Dalton Bertolim

; Oliveira, Gláucia Maria Moraes de

; Simão, Antonio Felipe

; Dutra, Oscar Pereira

; Coelho, Otávio Rizzi

; Izar, Maria Cristina de Oliveira

; Póvoa, Rui Manuel dos Santos

; Giuliano, Isabela de Carlos Back

; Alencar Filho, Aristóteles Comte de

; Machado, Carlos Alberto

; Scherr, Carlos

; Fonseca, Francisco Antonio Helfenstein

; Santos Filho, Raul Dias dos

; Carvalho, Tales de

; Avezum Jr., Álvaro

; Esporcatte, Roberto

; Nascimento, Bruno Ramos

; Brasil, David de Pádua

; Soares, Gabriel Porto

; Villela, Paolo Blanco

; Ferreira, Roberto Muniz

; Martins, Wolney de Andrade

; Sposito, Andrei C.

; Halpern, Bruno

; Saraiva, José Francisco Kerr

; Carvalho, Luiz Sergio Fernandes

; Tambascia, Marcos Antônio

; Coelho-Filho, Otávio Rizzi

; Bertolami, Adriana

; Correa Filho, Harry

; Xavier, Hermes Toros

; Faria-Neto, José Rocha

; Bertolami, Marcelo Chiara

; Giraldez, Viviane Zorzanelli Rocha

; Brandão, Andrea Araújo

; Feitosa, Audes Diógenes de Magalhães

; Amodeo, Celso

; Souza, Dilma do Socorro Moraes de

; Barbosa, Eduardo Costa Duarte

; Malachias, Marcus Vinícius Bolívar

; Souza, Weimar Kunz Sebba Barroso de

; Costa, Fernando Augusto Alves da

; Rivera, Ivan Romero

; Pellanda, Lucia Campos

; Silva, Maria Alayde Mendonça da

; Achutti, Aloyzio Cechella

; Langowiski, André Ribeiro

; Lantieri, Carla Janice Baister

; Scholz, Jaqueline Ribeiro

; Ismael, Silvia Maria Cury

; Ayoub, José Carlos Aidar

; Scala, Luiz César Nazário

; Neves, Mario Fritsch

; Jardim, Paulo Cesar Brandão Veiga

; Fuchs, Sandra Cristina Pereira Costa

; Jardim, Thiago de Souza Veiga

; Moriguchi, Emilio Hideyuki

; Schneider, Jamil Cherem

; Assad, Marcelo Heitor Vieira

; Kaiser, Sergio Emanuel

; Lottenberg, Ana Maria

; Magnoni, Carlos Daniel

; Miname, Marcio Hiroshi

; Lara, Roberta Soares

; Herdy, Artur Haddad

; Araújo, Cláudio Gil Soares de

; Milani, Mauricio

; Silva, Miguel Morita Fernandes da

; Stein, Ricardo

; Lucchese, Fernando Antonio

; Nobre, Fernando

; Griz, Hermilo Borba

; Magalhães, Lucélia Batista Neves Cunha

; Borba, Mario Henrique Elesbão de

; Pontes, Mauro Ricardo Nunes

; Mourilhe-Rocha, Ricardo

Arquivos Brasileiros de Cardiologia

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oct 2019, Volume 113 N. 4 Pages 787 - 891

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190204 40841 downloads

Are there differences in the growth adaptation processes of growing and mature organism models of short bowel syndrome?
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Tannuri, Ana Cristina Aoun

; Rotondo, ĺtalo Geraldo

; Barros, Guilherme Garcia

; Vaisberg, Victor Van

; Mendes-Neto, Cícero

; Paes, Vitor Ribeiro

; Coelho, Maria Cecilia Mendonça

; Gonçalves, Josiane

; Serafini, Suellen

; Tannuri, Uenis

Clinics

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2018, Volume 73 elocation e499

Abstract: En | Text: En | PDF: En

OBJECTIVES: The purpose of this study was to present an experimental model of short bowel syndrome (SBS) in weaning rats and to compare the adaptative mechanisms of the remaining bowel in weaning rats and adult animals by means of morphometric, histologic and molecular methods. METHODS: Twenty-four weaning rats were divided into 3 groups of 8 animals, one control group and two short bowel groups (euthanasia after 4 and 21 days), and were compared with similar adult groups. Morphometric evaluations of the animals and histopathological and molecular studies of the remaining bowel were performed. RESULTS: The weight of young rats increased after enterectomy, whereas that of adult rats decreased after enterectomy (p<0.0001). The ratio of intestinal length/body weight was significantly higher in weaning rats than in adults (p<0.002), showing that intestinal growth was more intense in weaning rats. Intestinal resection promoted increased thickness of the small bowel lamina propria (p=0.001) and reduced thickness of the colon lamina propria (p=0.04) in weaning rats relative to those in adults. In addition, intestinal resection promoted increased expression of the Bcl-xl gene (antiapoptotic) in adult animals compared with that in weaning rats (p=0.001). CONCLUSION: Morphometric, histological and molecular differences were shown in the adaptation processes of growing and mature organisms.

https://doi.org/10.6061/clinics/2018/e499 282 downloads

Mechanisms of the beneficial effect of sevoflurane in liver ischemia/reperfusion injury
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Cavalcante, Fernanda Paula

; Coelho, Ana Maria Mendonça

; Machado, Marcel Cerqueira Cesar

; Sampietre, Sandra Nassa

; Patzina, Rosely Antunes

; Diniz, Márcio Augusto

; Chaib, Eleazar

; D'Albuquerque, Luiz Augusto Carneiro

Acta Cirúrgica Brasileira

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nov 2015, Volume 30 N. 11 Pages 749 - 755

Abstract: En | Text: En | PDF: En

PURPOSE: To evaluate the underlying mechanisms by which sevoflurane protects the liver against ischemia/reperfusion injury evaluate the mechanism by which sevoflurane exerts this protective effect. METHODS: Twenty-six rats were subjected to partial ischemia/reperfusion injury for 1h: one group received no treatment, one group received sevoflurane, and sham group of animals received laparotomy only. Four hours after reperfusion, levels of alanine and aspartate aminotransferases, tumor necrosis factor-a, and interleukins 6 and 10 were measured. Analyses of mitochondrial oxidation and phosphorylation, malondialdehyde content, histology, and pulmonary vascular permeability were performed. RESULTS: Serum levels of alanine and aspartate aminotransferases were significantly lower in the sevoflurane group compared to untreated controls (p<0.05). The sevoflurane group also showed preservation of liver mitochondrial function compared to untreated controls (p<0.05). Sevoflurane administration did not alter increases in serum levels of tumor necrosis factor-a, and interleukins 6 and 10. Sevoflurane treatment significantly reduced the coagulative necrosis induced by ischemia/reperfusion (p<0.05). Pulmonary vascular permeability was preserved in the sevoflurane group compared to untreated controls. CONCLUSION: Sevoflurane administration protects the liver against ischemia/reperfusion injury, via preservation of mitochondrial function, and also preserves lung vascular permeability.

https://doi.org/10.1590/S0102-865020150110000005 1791 downloads

Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ
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Santos, Maria Mercês

; Tannuri, Ana Cristina Aoun

; Coelho, Maria Cecilia Mendonça

; Gonçalves, Josiane de Oliveira

; Serafini, Suellen

; Silva, Luiz Fernando Ferraz da

; Tannuri, Uenis

Clinics

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may 2015, Volume 70 N. 5 Pages 373 - 379

Abstract: En | Text: En | PDF: En

OBJECTIVE: Intestinal ischemia-reperfusion injury occurs in several clinical conditions and after intestinal transplantation. The aim of the present study was to investigate the phenomena of apoptosis and cell proliferation in a previously described intestinal ischemia-reperfusion injury autograft model using immunohistochemical markers. The molecular mechanisms involved in ischemia-reperfusion injury repair were also investigated by measuring the expression of the early activation genes c-fos and c-jun, which induce apoptosis and cell proliferation. MATERIALS AND METHODS: Thirty adult male Wistar rats were subjected to surgery for a previously described ischemia-reperfusion model that preserved the small intestine, the cecum and the ascending colon. Following reperfusion, the cecum was harvested at different time points as a representative segment of the intestine. The rats were allocated to the following four subgroups according to the reperfusion time: subgroup 1: 5 min; subgroup 2: 15 min; subgroup 3: 30 min; and subgroup 4: 60 min. A control group of cecum samples was also collected. The expression of c-fos, c-jun and immunohistochemical markers of cell proliferation and apoptosis (Ki67 and TUNEL, respectively) was studied. RESULTS: The expression of both c-fos and c-jun in the cecum was increased beginning at 5 min after ischemia-reperfusion compared with the control. The expression of c-fos began to increase at 5 min, peaked at 30 min, and exhibited a declining tendency at 60 min after reperfusion. A progressive increase in c-jun expression was observed. Immunohistochemical analyses confirmed these observations. CONCLUSION: The early activation of the c-fos and c-jun genes occurred after intestinal ischemia-reperfusion injury, and these genes can act together to trigger cell proliferation and apoptosis.

https://doi.org/10.6061/clinics/2015(05)12 1530 downloads

Effects of ischemic preconditioning in a pig model of large-for-size liver transplantation
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Leal, Antonio José Gonçalves

; Tannuri, Ana Cristina Aoun

; Belon, Alessandro Rodrigo

; Guimarães, Raimundo Renato Nunes

; Coelho, Maria Cecília Mendonça

; Gonçalves, Josiane de Oliveira

; Serafini, Suellen

; Melo, Evandro Sobroza de

; Tannuri, Uenis

Clinics

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feb 2015, Volume 70 N. 2 Pages 126 - 135

Abstract: En | Text: En | PDF: En

OBJECTIVE: In most cases of pediatric liver transplantation, the clinical scenario of large-for-size transplants can lead to hepatic dysfunction and a decreased blood supply to the liver graft. The objective of the present experimental investigation was to evaluate the effects of ischemic preconditioning on this clinical entity. METHODS: Eighteen pigs were divided into three groups and underwent liver transplantation: a control group, in which the weights of the donors were similar to those of the recipients, a large-for-size group, and a large-for-size + ischemic preconditioning group. Blood samples were collected from the recipients to evaluate the pH and the sodium, potassium, aspartate aminotransferase and alanine aminotransferase levels. In addition, hepatic tissue was sampled from the recipients for histological evaluation, immunohistochemical analyses to detect hepatocyte apoptosis and proliferation and molecular analyses to evaluate the gene expression of Bax (pro-apoptotic), Bcl-XL (anti-apoptotic), c-Fos and c-Jun (immediate-early genes), ischemia-reperfusion-related inflammatory cytokines (IL-1, TNF-alpha and IL-6, which is also a stimulator of hepatocyte regeneration), intracellular adhesion molecule, endothelial nitric oxide synthase (a mediator of the protective effect of ischemic preconditioning) and TGF-beta (a pro-fibrogenic cytokine). RESULTS: All animals developed acidosis. At 1 hour and 3 hours after reperfusion, the animals in the large-for-size and large-for-size + ischemic preconditioning groups had decreased serum levels of Na and increased serum levels of K and aspartate aminotransferase compared with the control group. The molecular analysis revealed higher expression of the Bax, TNF-alpha, I-CAM and TGF-beta genes in the large-for-size group compared with the control and large-for-size + ischemic preconditioning groups. Ischemic preconditioning was responsible for an increase in c-Fos, IL-1, IL-6 and e-NOS gene expression. CONCLUSION: Ischemia-reperfusion injury in this model of large-for-size liver transplantation could be partially attenuated by ischemic preconditioning.

https://doi.org/10.6061/clinics/2015(02)10 1380 downloads

10.

Aplicabilidade da CIPE® fundamentada na teoria da modelagem e modelagem de papel
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Coelho, Manuela de Mendonça Figueirêdo

; Miranda, Karla Corrêa Lima

; Guedes, Maria Vilaní Cavalcante

; Monteiro, Ana Ruth Macêdo

; Silva, Lúcia de Fátima da

; Leite, Ana Claúdia de Souza

Revista Brasileira de Enfermagem

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jun 2014, Volume 67 N. 3 Pages 438 - 442

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

Objetivou-se analisar a proposta de plano educativo organizado por meio do processo de enfermagem, utilizando a Classificação Internacional para a Prática de Enfermagem (CIPE®), fundamentada pela Teoria da Modelagem e Modelagem de Papel, a um grupo de mulheres em Maracanaú-CE, Brasil. Trata-se de um estudo de caso realizado em grupo educativo de mulheres. A abordagem qualitativa conduziu a produção dos dados guiados pelo Método Criativo e Sensível. Dois diagnósticos centrais emergiram das necessidades apresentadas pelo grupo: saúde comprometida e conhecimento adequado, assim como formulação de resultados e intervenções de enfermagem. O estudo aponta a possibilidade de aplicação do processo de enfermagem apoiado pela CIPE® e ações educativas baseadas na Teoria da Modelagem e Modelagem de Papel.

This study aimed to analyze the proposal of educational plan organized through the Nursing Process, using the International Classification for Nursing Practice (ICNP®), based on the Theory of Modeling and Role Modeling, to one group of women in Maracanaú-CE, Brazil. A case study was carried out in an educational group of women. A qualitative approach conducted the data production guided by the Creative and Sensitive Method. Two main diagnoses emerged from the needs presented by the group: compromised health and adequate knowledge, as well as formulation of results and nursing interventions. This study indicates the possibility of Nursing Process supported by INCP® and educational activities based on the Theory of Modeling and Role Modeling.

Objetivó-se analizar la propuesta de plan educativo organizado por el Proceso de Enfermería, utilizando la Clasificación Internacional para la Práctica de Enfermería, basada en la Teoría de Modelaje y Modelaje de Papel, a un grupo de mujeres en Maracanaú-CE, Brasil. Un estudio de caso fue realizado en grupo de educación a mujeres. El enfoque cualitativo ha dado lugar a la producción de datos guiados por el Método Creativo y Sensible. Dos diagnósticos centrales emergieron de las necesidades presentadas por el grupo: salud comprometida y conocimiento adecuado, así como formulación de resultados e intervenciones de enfermería. Se indica la posibilidad de aplicación de lo Proceso de Enfermería con el apoyo de la Clasificación Internacional para la Práctica de Enfermería y acciones educativas basadas en la Teoría de Modelaje y Modelaje de Papel.

https://doi.org/10.5935/0034-7167.20140058 4637 downloads

11.

Aplicabilidade da CIPE® fundamentada na teoria da modelagem e modelagem de papel
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Coelho, Manuela de Mendonça Figueirêdo

; Miranda, Karla Corrêa Lima

; Guedes, Maria Vilaní Cavalcante

; Monteiro, Ana Ruth Macêdo

; Silva, Lúcia de Fátima da

; Leite, Ana Claúdia de Souza

Revista Brasileira de Enfermagem

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jun 2014, Volume 67 N. 3 Pages 438 - 442

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

https://doi.org/10.5935/0034-7167.20140058 2 downloads

12.

MOTIVOS DA NÃO ADESÃO DE MÃES AO ESQUEMA VACINAL DE FILHOS EXPOSTOS AO HIV
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Sherlock, Maria do Socorro Mendonça

; Cardoso, Maria Vera Lúcia Moreira Leitão

; Lélis, Ana Luíza Paula de Aguiar

; Lopes, Márcia Maria Coelho Oliveira

; Pereira, Maria Lúcia Duarte

Rev Rene

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2013, Volume 14 N. 2 Pages 341 - 353

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

We aimed to describe the knowledge of HIV seropositive mother on the children immunization schedule and know the reasons for their non-compliance to the children immunization schedule. This is a descriptive study with qualitative approach carried out in the outpatient clinic of a public institution, in Fortaleza-CE, Brazil. The subjects were 16 mothers or caregivers of children up to two years of age exposed to HIV/AIDS. Data collection happened through semi-structured interviews conducted from January to March 2012. Two categories emerged from the analysis: Lack of knowledge of mother about special immunization schedule for children with HIV and Reasons for non-compliance to special immunization schedule. Mothers presented poor knowledge, fear and disinterest in the information on the disease and preventive measures, as well as the difficulties faced. Health professionals should promote strategies with these mothers seeking integration, commitment and comprehension of the importance of immunization in children.

El objetivo fue describir el conocimiento de madre seropositiva al VIH sobre el calendario de vacunación del hijo y conocer las razones de no adhesión al esquema de vacunas del hijo. Estudio descriptivo, cualitativo, llevado a cabo en ambulatorio de institución pública de Fortaleza-CE, Brasil. Participaron 16 madres/cuidadoras de niños con hasta dos años expuestos al VIH/SIDA. Los datos fueron recolectados a través de entrevista semiestructurada, de enero a marzo/2012. Del análisis, emergieron dos categorías: desconocimiento de la madre acerca de las vacunas especiales en niños con VIH y razones de la no adherencia al programa de vacunación especial. Las madres presentaron deficiencia de conocimiento, señalaron miedo y desinterés por informaciones sobre la enfermedad y las medidas preventivas, además de dificultades enfrentadas. Los profesionales de salud deben promover acciones con estas madres para integración, responsabilidad y atención acerca de la importancia de las vacunas a la salud del hijo.

Objetivou-se descrever o conhecimento da mãe soropositiva para HIV sobre o calendário de vacinação do filho e conhecer os motivos da não adesão ao esquema vacinal do filho. Estudo descritivo, abordagem qualitativa realizado no ambulatório de uma instituição pública, em Fortaleza-CE. Os sujeitos constituíram-se de 16 mães/cuidadoras de crianças de até dois anos de idade, expostas ao HIV/AIDS. Os dados foram coletados por meio de entrevista semiestruturada aplicada de janeiro a março/2012. Da análise, emergiram duas categorias: desconhecimento da mãe sobre as vacinas do esquema especial em crianças com HIV, motivos da não adesão ao esquema vacinal especial. As mães apresentaram deficiência de conhecimento, demonstraram medo e desinteresse pelas informações acerca da doença e das medidas preventivas, bem como dificuldades enfrentadas. Os profissionais de saúde devem promover estratégias junto a essas mães visando integração, compromisso e entendimento sobre a importância da vacinação para saúde do filho.

https://doi.org/10.15253/2175-6783.2013000200013 51 downloads

13.

A simplified experimental model of large-for-size liver transplantation in pigs
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Leal, Antonio Jose Goncalves

; Tannuri, Ana Cristina Aoun

; Belon, Alessandro Rodrigo

; Guimaraes, Raimundo Renato Nunes

; Coelho, Maria Cecilia Mendonca

; de Oliveira Goncalves, Josiane

; Sokol, Suellen Serafini

; De Melo, Evandro Sobroza

; Otoch, Jose Pinhata

; Tannuri, Uenis

Clinics

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2013, Volume 68 N. 8 Pages 1152 - 1156

Abstract: En | Text: En | PDF: En

OBJECTIVE: The ideal ratio between liver graft mass and recipient body weight for liver transplantation in small infants is unknown; however, if this ratio is over 4%, a condition called large-for-size may occur. Experimental models of large-for-size liver transplants have not been described in the literature. In addition, orthotopic liver transplantation is marked by high morbidity and mortality rates in animals due to the clamping of the venous splanchnic system. Therefore, the objective of this study was to create a porcine model of large-for-size liver transplantation with clamping of the supraceliac aorta during the anhepatic phase as an alternative to venovenous bypass. METHOD: Fourteen pigs underwent liver transplantation with whole-liver grafts without venovenous bypass and were divided into two experimental groups: the control group, in which the weights of the donors were similar to the weights of the recipients; and the large-for-size group, in which the weights of the donors were nearly 2 times the weights of the recipients. Hemodynamic data, the results of serum biochemical analyses and histological examination of the transplanted livers were collected. RESULTS: The mortality rate in both groups was 16.5% (1/7). The animals in the large-for-size group had increased serum levels of potassium, sodium, aspartate aminotransferase and alanine aminotransferase after graft reperfusion. The histological analyses revealed that there were no significant differences between the groups. CONCLUSION: This transplant method is a feasible experimental model of large-for-size liver transplantation.

https://doi.org/10.6061/clinics/2013(08)15 1795 downloads

14.

Effects of the administration of pentoxifylline and prednisolone on the evolution of portal fibrogenesis secondary to biliary obstruction in growing animals: immunohistochemical analysis of the expression of TGF-β and VEGF
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Andrade, Wagner de Castro

; Silva, Luiz Fernando Ferraz da

; Coelho, Maria Cecilia de Mendonça

; Tannuri, Ana Cristina Aoun

; Alves, Venancio Avancini Ferreira

; Tannuri, Uenis

Clinics

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dec 2012, Volume 67 N. 12 Pages 1455 - 1461

Abstract: En | Text: En | PDF: En

OBJECTIVE: During the neonatal and infancy periods, some chronic liver diseases may lead to progressive hepatic fibrosis, which is a condition that can ultimately result in the loss of organ function and severe portal hypertension necessitating hepatic transplantation. In a previous report, pharmacological interventions were demonstrated to modulate hepatic fibrosis induced by bile duct ligation in young rats. The administration of pentoxifylline or prednisolone, or the combination of both, resulted in reduced fibrogenesis in portal spaces. The objectives of the present study were to evaluate the expression of transforming growth factor β and vascular endothelial growth factor after bile duct ligation in young rats and to assess the effect of those same drugs on cytokine expression. METHODS: In this experimental study, 80 young rats (21 or 22 days old) were submitted either to laparotomy and common bile duct ligation or to sham surgery. The animals were allocated into four groups according to surgical procedure, and the following treatments were administered: (1) common bile duct ligation + distilled water, (2) sham surgery + distilled water, (3) common bile duct ligation + pentoxifylline, or (4) common bile duct ligation + prednisolone. After 30 days, a hepatic fragment was collected from each animal for immunohistochemical analysis using monoclonal antibodies against transforming growth factor β and vascular endothelial growth factor. Digital morphometric and statistical analyses were performed. RESULTS: The administration of pentoxifylline reduced the transforming growth factor β-marked area and the amount of transforming growth factor β expressed in liver tissue. This effect was not observed after the administration of prednisolone. There was a significant reduction in vascular endothelial growth factor expression after the administration of either drug compared with the non-treatment group. CONCLUSIONS: The administration of pentoxifylline to cholestatic young rats resulted in the diminished expression of transforming growth factor β and vascular endothelial growth factor in liver tissue. The administration of steroids resulted in the diminished expression of vascular endothelial growth factor only. These pathways may be involved in hepatic fibrogenesis in young rats submitted to bile duct ligation and exposed to pentoxifylline or prednisolone.

https://doi.org/10.6061/clinics/2012(12)17 1433 downloads

15.

Do opioid receptors play a role in the pathogenesis of the inflammatory response in acute pancreatitis?
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Penido, Artur

; Coelho, Ana Maria Mendonça

; Molan, Nilza Trindade

; Silva, Fabiano Pinheiro da

; D'Albuquerque, Luiz Augusto Carneiro

; Machado, Marcel Cerqueira Cesar

Acta Cirúrgica Brasileira

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sep 2012, Volume 27 N. 9 Pages 600 - 605

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Investigar o efeito do bloqueador opióide naltrexone na resposta inflamatória da pancreatite aguda. METODOS: Pancreatite aguda foi induzida em ratos machos Wistar, através de injeção retrógada de solução de taurocolato de sódio a 2,5% nos ductos pancreáticos. Os animais foram alocados em dois grupos: Grupo controle (n=9) animais receberam 0,5 ml de solução salina intra-peritonial 15 minutos antes da indução da pancreatite aguda e Grupo naltrexone (n=9) animais receberam naltrexone (15mg/kg de peso), em 0,5 ml de volume final por via intraperitoneal, 15 minutos antes da indução da pancreatite aguda. Foram avaliados o volume de ascite, os níveis séricos de amilase e IL-6, assim como TNF-α peritoneal e a atividade da mieloperoxidase (MPO) no tecido pulmonar. RESULTADOS: Não foram encontradas diferenças significantes nos parâmetros analisados entre o grupo que recebeu solução salina e o que recebeu naltrexone . CONCLUSÕES: Os receptores opióides não desempenham papel importante na resposta inflamatória sistêmica associada à pancreatite aguda. Se os opioides alteram a sinalização inflamatória nos leucócitos está ação não se reflete na patogênese da pancreatite aguda

PURPOSE: To investigate the effect of the opioid blocker naltrexone in the inflammatory response in acute pancreatitis (AP). METHODS: Acute pancreatitis was induced in anesthetized male Wistar rats by retrograde injection of 2.5% sodium taurocholate diluted in 0.5ml saline into the main pancreatic duct. Animals were randomized to the following experimental groups: Control Group (n=9): animals received an intraperitoneal injection of saline solution (0.5ml), 15 minutes before the induction of AP. Naltrexone Group (n=9): animals received an intraperitoneal injection of naltrexone 0.5ml (15 mg/kg), 15 minutes before induction of AP. Peritoneal levels of TNF-α and serum levels of IL-6 and amylase were determined The volume of the ascitic fluid was also evaluated. Myeloperoxidase (MPO) activities were analyzed in homogenates of pulmonary tissue. RESULTS: There were no significant differences in the ascitic fluid volume, nor in TNF-a and IL-6 levels in the naltrexone group compared to controls. Treatment with naltrexone did not affect the lung MPO activity compared to control group. CONCLUSIONS: The opioid receptors don't play an important role in the pathogenesis of the inflammatory response in acute pancreatitis. If opioids affect leukocytes inflammatory signaling, there are no major implications in the pathogenesis of acute pancreatitis.

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