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Resumo A sexagem das espécies é uma ferramenta importante para o manejo populacional. Em tartarugas, cágados e jabutis, a sexagem geralmente é feita de acordo com características secundárias, como morfologia do plastrão ou técnicas de eversão do falo. Neste artigo, apresentamos o método de eversão do falo e comparamos sua eficiência com outras técnicas existentes. Este novo método visa encurtar o tempo de manejo e reduzir os efeitos colaterais físicos nos animais, promovendo o manejo clínico e reprodutivo do cágado-de-barbicha (Phrynops geoffroanus) e espécies relacionadas. Uma amostra de cinquenta indivíduos foi coletada na região central do estado do Piauí, Nordeste do Brasil. O método proposto consiste em remover a cabeça de sua posição de repouso. Os métodos que obtiveram índice de sucesso superior a 80% foram comparados entre si quanto ao tempo de estimulação necessário para a eversão do falo. O método aqui proposto alcançou 100% de eficiência na determinação do sexo de espécimes com comprimento de carapaça linear entre 10 e 34 cm. Além disso, quando comparado ao segundo método mais eficiente, reduziu substancialmente o tempo necessário para o manejo dos espécimes (91%), evitando possíveis sequelas nos indivíduos manuseados.

Abstract The sexing of species is an important tool for population management. In tortoises and turtles, sexing is usually done according to secondary characteristics, such as plastron morphology or phallus eversion techniques. In this paper, we present the phallus eversion method and compare its efficiency to other existing techniques. This new method aims at shortening the management time and reducing the physical aftereffects on the animals, fostering the clinical and reproductive management of Geoffroy’s side-necked turtle (Phrynops geoffroanus) and related species. A sample of fifty individuals was taken in the central region of Piauí state, Northeastern Brazil. The proposed method consisted of moving the head from its resting position. The methods achieving a success rate higher than 80% were compared to each other as for the stimulation time required for phallus eversion. The method as here proposed achieved 100% efficiency in the sex determination of specimens with a linear carapace length between 10 and 34 cm. Also, when compared to the second most efficient method, it substantially reduced the time needed for managing the specimens (91%), avoiding possible sequelae in the individuals handled.

https://doi.org/10.1590/1519-6984.249808 204 downloads

Long-term oncological and surgical outcomes after Video Endoscopic Inguinal Lymphadenectomy (VEIL) in patients with penile cancer
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Tobias-Machado, Marcos

; Ornellas, Antonio A.

; Hidaka, Alexandre K.

; Medina, Luis G.

; Mattos, Pablo A. L.

; Besio, Ruben S.

; Abreu, Diego

; Castro, Pedro R.

; Nishimoto, Ricardo H.

; Astigueta, Juan

; Dourado, Aurus

; Machado, Roberto D.

; Magnabosco, Wesley J.

; Corona-Montes, Victor

; Villoldo, Gustavo M.

; Zampolli, Hamilton C.

; Taha, Anis

; Auad, Pericles R.

; Faria, Eliney F.

; Arantes, Paulo B. O.

; Tavares, Alessandro

; Nascimento, Francisco S. M. S.

; Brazão Jr., Eder S.

; Rocha, Maurício M.

; Costa, Walter H.

; Panico, Vinicius

; Reis, Leonardo O.

; Almeida-Carrera, Roberto J.

; Silva, Rafael C.

; Zequi, Stênio C.

; Calixto, José R. R.

; Sotelo, Rene

International braz j urol

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oct 2023, Volume 49 N. 5 Pages 580 - 589

Abstract: En | Text: En | PDF: En

ABSTRACT Objective: To report outcomes from the largest multicenter series of penile cancer patients undergoing video endoscopic inguinal lymphadenectomy (VEIL). Materials and Methods: Retrospective multicenter analysis. Authors of 21 centers from the Penile Cancer Collaborative Coalition-Latin America (PeC-LA) were included. All centers performed the procedure following the same previously described standardized technique. Inclusion criteria included penile cancer patients with no palpable lymph nodes and intermediate/high-risk disease and those with non-fixed palpable lymph nodes less than 4 cm in diameter. Categorical variables are shown as percentages and frequencies whereas continuous variables as mean and range. Results: From 2006 to 2020, 210 VEIL procedures were performed in 105 patients. Mean age was 58 (45-68) years old. Mean operative time was 90 minutes (60-120). Mean lymph node yield was 10 nodes (6-16). Complication rate was 15.7%, including severe complications in 1.9% of procedures. Lymphatic and skin complications were noted in 8.6 and 4.8% of patients, respectively. Histopathological analysis revealed lymph node involvement in 26.7% of patients with non-palpable nodes. Inguinal recurrence was observed in 2.8% of patients. 10y- overall survival was 74.2% and 10-y cancer specific survival was 84.8%. CSS for pN0, pN1, pN2 and pN3 were 100%, 82.4%, 72.7% and 9.1%, respectively. Conclusion: VEIL seems to offer appropriate long term oncological control with minimal morbidity. In the absence of non-invasive stratification measures such as dynamic sentinel node biopsy, VEIL emerged as the alternative for the management of non-bulky lymph nodes in penile cancer.

https://doi.org/10.1590/s1677-5538.ibju.2023.0065

Gastroesophageal tube of the Iguana iguana (Iguanidae): histological description, histochemical and immunohistochemical analysis of 5-HT and SS cells
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Serra-Campos, A. O.

; Abreu-Junior, A. N. G.

; Nascimento, A. A.

; Abidu-Figueiredo, M.

; Lima, M. S. C. S.

; Machado-Santos, C.

Brazilian Journal of Biology

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2023, Volume 83 elocation e242086

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Os objetivos do trabalho foram descrever a estrutura histológica e histoquímica do tubo gastroesofágico da Iguana iguana e verificar a ocorrência e distribuição de células serotonina (5-HT) e somatostatina (SS) imunorreativas. Fragmentos do trato gastrointestinal (TGI) de cinco iguanas foram submetidos à técnica histológica e imunohistoquímica padrão. As células imunorreativas para 5-HT e SS foram quantificadas usando o STEPanizer. O esôfago apresenta epitélio pseudoestratificado colunar ciliado Alcian blue (AB) positivo, com células caliciformes altamente reativas ao ácido periódico de Schiff (PAS). No esôfago cervical, a densidade numérica de células 5-HT por unidade de área (QA [células 5-HT] / µm2) foi de 4.6x10-2 ± 2.0 e o esôfago celomático apresentou QA = 4.0x10-2 ± 1.0. O epitélio do estômago é colunar simples, PAS e AB positivo. As regiões cranial e média do estômago apresentaram (QA [células 5-HT] / µm2) = 6.18x10-2 ± 3.2 e a região caudal, QA = 0.6x10-2 ± 0.2. As células SS foram observadas apenas no estômago caudal, com densidade numérica (QA [células SS] / µm2) = 1.4x10-2 ± 0.9. Em I. iguana, foi observada variações em termos da distribuição das secreções de muco e padrão de ocorrência das células enteroendócrinas secretoras de serotonina e somatostatina no TGI, o que possivelmente reflete uma resposta adaptativa interespecifica.

Abstract The work aims were to describe the histological and histochemical structure of the gastroesophageal tube of Iguana iguana and verify the occurrence and distribution of immunoreactive serotonin (5-HT) and somatostatin (SS) cells. Fragments of the gastrointestinal tract (GIT) of five iguanas were which underwent standard histological and immunohistochemistry technique. Immunoreactive cells for 5-HT and SS were quantified using the STEPanizer. The oesophagus has ciliated columnar pseudostratified epithelium with staining Alcian blue (AB) + and goblet cells highly reactive to periodic acid Schiff (PAS). In the cervical oesophagus, the numerical density of 5-HT cells per unit area (QA [5-HT cells]/µm2) was 4.6x10-2 ± 2.0 and celomatic oesophagus presented QA = 4.0x10-2 ± 1.0. The epithelium of the stomach is simple columnar, PAS and AB +. The cranial and middle regions of the stomach presented (QA [5-HT cells]/µm2) = 6.18x10-2 ± 3.2 and the caudal region, QA = 0.6x10-2 ± 0.2. The SS cells were only observed in the caudal stomach, with numerical density (QA [SS cells]/µm2) = 1.4x10-2 ± 0.9 In I. iguana, variation was observed in terms of the distribution of mucus secretions and the pattern of occurrence of serotonin and somatostatin-secreting enteroendocrine cells in the TGI, which possibly will result in an interspecific adaptive response.

https://doi.org/10.1590/1519-6984.242086 370 downloads

Biogenic synthesis of silver nanoparticles from thyme extract for functionalization of a bioactive glass produced from rice husk ash
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Pereira, M. A.

; Oliveira, J. E. de

; Abreu, D. J. M. de

; Piccoli, R. H.

; Fonseca, C. S.

Cerâmica

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dec 2022, Volume 68 N. 388 Pages 469 - 474

Abstract: En | Text: En | PDF: En

Abstract The rice husk, agro-industrial residue, stands out for having silica, the main oxide former of the bioactive glass. To avoid contamination in this biomaterial, substances with bactericidal properties, like silver can be incorporated. Thus, the objective of this work is the synthesis of silver nanoparticles to functionalize a bioactive glass synthesized from rice husk ash (BRHA). The bioglass was prepared by the sol-gel method from the rice husk ash as a silica source. Silver nanoparticles were synthesized from thyme extract with AgNO3 and their formation was verified by UV-vis spectroscopy. The glass powder was added to this solution and its structure was evaluated by SEM-EDS, ATR-FTIR, and microbiology. The bioglass powder appeared in the form of porous agglomerates and the addition of silver altered its superficial roughness by deposition. The chemical bonds of the glass network related to bioactivity were maintained after the incorporation of the nanoparticles. Microbiological analysis by the agar diffusion test confirmed the inhibitory action of the nanoparticles for P. aeruginosa and S. aureus. Therefore, the developed material presented great potential for biomedical applications.

https://doi.org/10.1590/0366-69132022683883323

Synthesis of Eugenol-Fluorinated Triazole Derivatives and Evaluation of Their Fungicidal Activity
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Lima, Ângela M. A.

; Paula, Wenderson T. de

; Leite, Iris C. H. L.

; Gazolla, Poliana A. R.

; Abreu, Lucas M. de

; Fonseca, Victor R.

; Romão, Wanderson

; Lacerda Jr., Valdemar

; Queiroz, Vagner T. de

; Teixeira, Róbson R.

; Costa, Adilson V.

Journal of the Brazilian Chemical Society

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oct 2022, Volume 33 N. 10 Pages 1200 - 1210

Abstract: En | Text: En | PDF: En

Eugenol (C10H12O2, 4-allyl-2-methoxyphenol) is a phenolic natural product that has several biological activities and possibilities of applications. It is herein described the synthesis of eugenol-fluorinated triazole derivatives and evaluation of their fungicidal activity. The reaction of eugenol with epichlorohydrin resulted in the preparation of (±)-2-((4-allyl-2-methoxyphenoxy)methyl)oxirane (1) in 88% yield. The azidolysis of 1 with sodium azide gave the azido-alcohol (±)-1-(4-allyl-2-methoxyphenoxy)-3-azidopropan-2-ol (2) in 94% yield. The CuAAc reaction between compound 2 and different terminal alkynes afforded a series of eleven derivatives (3a 3k) within 48-80% yield. All the synthesized compounds were characterized by infrared (IR) and nuclear magnetic resonance (1H and 13C) spectroscopies and high-resolution mass spectrometry. The in vitro inhibitory activity of the compounds on the mycelial growth of a strain of Colletotrichum sp., that causes anthracnose disease on papaya fruits, was evaluated. The best result was observed for compound 1-(4-allyl-2-methoxyphenoxy)-3-(4-(2-fluorophenyl)-1H-1,2,3-triazol-1-yl)propan-2-ol (3d) that showed a mean growth-inhibition zone of 5.10 mm in a well-diffusion assay, and may serve as a template for additional structural modifications, aiming for more potent fungicidal activity.

https://doi.org/10.21577/0103-5053.20220040

Horismenus cupreus (Hymenoptera: Eulophidae) parasitizing Bedellia somnulentella (Lepidoptera: Bedelliidae) in Ipomoea batatas
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Santos, M. M.

; Cabral, M. J. D. S.

; Abreu, C. M.

; Silva, I. M. da

; Costa, V. A.

; Demolin-Leite, G. L.

; Pires, E. M.

; Soares, M. A.

Brazilian Journal of Biology

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2022, Volume 82 elocation e262626

Abstract: En | Text: En | PDF: En

https://doi.org/10.1590/1519-6984.262626

Customer satisfaction in service delivery with artificial intelligence: A meta-analytic study
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Aguiar-Costa, Laura M.

; Cunha, Carlos A. X. C.

; Silva, Wallysson K. M.

; Abreu, Nelsio R.

RAM. Revista de Administração Mackenzie

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2022, Volume 23 N. 6 elocation eRAMD220003

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: O presente estudo tem como objetivo identificar os principais construtos antecedentes e consequentes que compõem a satisfação do consumidor na prestação de serviços que utilizam inteligência artificial (IA) e suas magnitudes. Originalidade/valor: Este estudo busca preencher uma lacuna advinda da escassez de pesquisas meta-analíticas sobre a prestação de serviços com IA e sua relação com a satisfação do consumidor. Design/metodologia/abordagem: O presente trabalho adotou o método meta-analítico e o seu desenvolvimento seguiu três fases: 1. pesquisa; 2. coleta; e 3. codificação e análise de dados. Foram analisados 19 artigos publicados em periódicos de relevância internacional no período de janeiro de 2000 e dezembro de 2020, presentes nas plataformas Web of Science e Science Direct, que totalizaram 128 observações e 28 relações. Resultados: Foram identificados cinco construtos antecedentes e um construto consequente. A partir disso, construiu-se um modelo integrado para ilustrar as relações da satisfação do consumidor em serviços inteligentes. Os resultados alcançados demonstram que a satisfação do consumidor na prestação de serviços está significantemente correlacionada a adoção de inteligência artificial. Em suma, a avaliação quantitativa integrada que foi realizada neste estudo, visa contribuir com futuras evidências empíricas de tal maneira que ocorra um aumento do alcance dos estudos em inteligência artificial e satisfação do consumidor, a partir da análise dos seguintes construtos: valor percebido, recursos percebidos, percepção da qualidade, orientação mercadológica, identificação com o serviço e comportamento de uso de AI em serviços.

ABSTRACT Purpose: This study intends to identify the main background and consequent constructs that form consumer satisfaction in providing services using artificial intelligence (AI) and their magnitudes. Originality/value: This work seeks to fill a gap arising from the scarcity of meta-analytic research on service delivery with AI and also its relationship to consumer satisfaction. Design/methodology/approach: The study adopted the meta-analytic method, and its development followed three phases: 1. research; 2. collection; and 3. coding and data analysis. We analyzed 19 articles published in journals of international relevance from January 2000 to December 2020, present on the Web of Science and Science Direct platforms, totaling 128 observations and 28 topic-related. Findings: Five background constructs and one consequent construct were identified, from which an integrated model was built to illustrate the relationships between consumer satisfaction in intelligent services. The results show that consumer satisfaction in the provision of services is significantly correlated to the adoption of artificial intelligence. Then, the integrated quantitative evaluation that was performed in this study aims to contribute to future empirical evidence in such a way that an increase in the scope of studies on artificial intelligence and consumer satisfaction occurs, based on the analysis of the following constructs: perceived value, perceived features, perception of quality, marketing orientation, identification with the service and behavior of using AI in services.

https://doi.org/10.1590/1678-6971/eramd220003.en

PREPARATION OF HYBRID POLYMERS AND USE AS CONTROLLED RELEASE DELIVERY VEHICLE FOR VOLATILE REPELLENT TO CONTROL Aedes aegypti
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Oliveira, Ygor M. de

; Delfino, Ângladis V.

; Carvalho, Cenira M. de

; Conserva, Lucia M.

; Abreu, Fabiane C. de

Química Nova

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apr 2021, Volume 44 N. 4 Pages 425 - 431

Abstract: En | Text: En | PDF: En

The Zika virus is a flavivirus spread by the Aedes aegypti mosquito, which also transmits dengue and chikungunya. Current forms of control include elimination of mosquito breeding sites, use of insecticides, repellents and other means. The present study developed new adsorbent materials based on silica and biomaterials capable of releasing an Aedes aegypti mosquito repellent. The materials synthesized were characterized by Fourier-Transform Infrared, thermogravimetric analysis, Scanning Electron Microscopy and Brunauer, Emmett and Teller analysis and tested with citronella, an essential oil widely recognized as a natural insect repellent. The materials presented type IV isotherms, with type H1 hysteresis, characteristic of mesoporous materials, with pore diameters of between 50 Å and 80 Å. Materials based on silica-chitosan-glutaraldehyde dried in an oven (SCGO) and silica-chitosan-glutaraldehyde dried under a vacuum (SCGV) were found to produce more controlled release of citronella essential oil. The bioassay test was performed using a horizontal double-choice olfactometer. The adsorbent SCGO remained stable for the four hours of the bioassay, while the commercial repellent became ineffective after two hours of release. The production of a prototype in the form of bracelets, stickers releasing a volatile repellent is therefore proposed as a method for the control of the mosquito in urban areas.

https://doi.org/10.21577/0100-4042.20170698 70 downloads

Reusable Heterogeneous SnO2/ZnO Catalyst for Biodiesel Production from Acidified/Acid Oils
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Dall’Oglio, Daiane F.

; Garcia, Marco A. S.

; Fiorio, Jhonatan L.

; Abreu, Wiury C. de

; Pereira, Laise N. S.

; Braga, Adriano

; Moura, Edmilson M. de

; Guldhe, Abhishek

; Bux, Faizal

; Moura, Carla V. R. de

Journal of the Brazilian Chemical Society

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jan 2021, Volume 32 N. 1 Pages 182 - 193

Abstract: En | Text: En | PDF: En

A catalyst comprised of SnO2 impregnated on ZnO nanowires, which presented remarkable ability to catalyze fatty acid esterification/transesterification reactions, is reported. For optimization of reaction conditions, artificially acidified soybean oil with 10 wt.% oleic acid was used as a model feed. The optimized conditions were: 150 °C, 6 h, 5 g of oil, catalyst concentration of 5%, and methanol:oil molar ratio of 15:1. The catalyst achieved 92% of total fatty acid methyl esters (FAME) content and was used five times without the necessity of catalyst washing from one reaction to the other. Then, such conditions were applied to produce biodiesel from the oil extracted from Scenedesmus sp. microalgae; the system reached 72% of FAME content, without any previous refining or degumming process of the oil. Rietveld refinement, X-ray diffraction, elemental mapping in scanning transmission electron microscopy, X-ray photoelectron spectroscopy, and pyridine-desorption Fourier-transform infrared spectroscopy were used to characterize the material.

https://doi.org/10.21577/0103-5053.20200167 355 downloads

10.

The effect of continuous positive airway pressure on blood pressure in patients with obstructive sleep apnea and uncontrolled hypertension - Study design and challenges during the COVID-19 pandemic
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Cruz, Fernanda C.S.G.

; Drager, Luciano F.

; Queiróz, Daniel B.C.

; Souza, Gabriela A.

; Pedrosa, Rodrigo P.

; Patriota, Tarcya L.G Couto

; Dórea, Egidio L.

; Vieira, Marcelo Luiz C.

; Righi, Camila G.

; Martinez, Denis

; Silva, Geruza A. da

; Silva, Giovanio V.

; Pio-Abreu, Andrea

; Lotufo, Paulo A.

; Benseãor, Isabela M.

; Bortolotto, Luiz A.

; Fuchs, Flávio D.

; Lorenzi-Filho, Geraldo

Clinics

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2021, Volume 76 elocation e2926

Abstract: En | Text: En | PDF: En

OBJECTIVES: To describe the MORPHEOS (Morbidity in patients with uncontrolled HTN and OSA) trial, and describe the challenges imposed by the COVID-19 pandemic. METHODS: MORPHEOS is a multicenter (n=6) randomized controlled trial designed to evaluate the blood pressure (BP) lowering effects of treatment with continuous positive airway pressure (CPAP) or placebo (nasal strips) for 6 months in adult patients with uncontrolled hypertension (HTN) and moderate-to-severe obstructive sleep apnea (OSA). Patients using at least one antihypertensive medication were included. Uncontrolled HTN was confirmed by at least one abnormal parameter in the 24-hour ABPM and ≥80% medication adherence evaluated by pill counting after the run-in period. OSA was defined by an apnea-hypopnea index ≥15 events/hours. The co-primary endpoints are brachial BP (office and ambulatory BP monitoring, ABPM) and central BP. Secondary outcomes include hypertension-mediated organ damage (HMOD) to heart, aorta, eye, and kidney. We pre-specified several sub-studies from this investigation. Visits occur once a week in the first month and once a month thereafter. The programmed sample size was 176 patients but the pandemic prevented this final target. A post-hoc power analysis will be calculated from the final sample. ClinicalTrials.gov: NCT02270658. RESULTS: The first 100 patients are predominantly males (n=69), age: 52±10 years, body mass index: 32.7±3.9 kg/m2 with frequent co-morbidities. CONCLUSIONS: The MORPHEOS trial has a unique study design including a run-in period; pill counting, and detailed analysis of hypertension-mediated organ damage in patients with uncontrolled HTN that will allow clarification of the impact of OSA treatment with CPAP.

https://doi.org/10.6061/clinics/2021/e2926 145 downloads

11.

Growth curve of selectively bred and non-selectively bred tambaqui (Colossoma macropomum)
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MARCOS, REBECA

; RIBEIRO, RICARDO P.

; ABREU, JANESSA S. DE

; FORNARI, DARCI C.

; OLIVEIRA, CARLOS A.L. DE

; STREIT JR, DANILO P.

; BARROS, CAIO M.C.A. DE

; LOPERA-BARRERO, NELSON M.

; CORRÊA FILHO, RUY A.C.

; POVH, JAYME A.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 3 elocation e20190099

Abstract: En | Text: En | PDF: En

Abstract The aim of this study was to evaluate the growth curve of selectively bred and non-selectively bred tambaqui (Colossoma macropomum). The experiment involved 388 fish (weight: 65.38 ± 20.00 g; age: 217 days), consisting of 252 fish from seven selectively bred families (18 fish per family) and 18 non-selectively bred fish (control group). Groups were placed in two 800-m² tanks. Biometric measurements were taken on nine occasions at 30-day intervals, for a period of 254 days. Weight and morphometric traits were evaluated. To describe the tambaqui growth behavior, we adopted the Gompertz nonlinear regression model. Greater growth (p < 0.05) was observed in selectively bred families compared with control group. Four families stood out with higher (p < 0.05) asymptotic values for weight (F1: 2448.7 g; F7: 2284.7 g; F5 2180.1 g; F4: 2080.5 g; and control: 1808.4 g) and other morphometric traits. None of the selectively bred families (except F5) had a higher growth rate and age at inflection point than the fish from control group. In conclusion, selectively bred and non-selectively bred fish present distinct growth curves, but some families have greatly superior growth.

https://doi.org/10.1590/0001-3765202020190099 527 downloads

12.

In Silico studies of novel Sildenafil self-emulsifying drug delivery system absorption improvement for pulmonary arterial hypertension
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ABRAHIM-VIEIRA, BARBÁRA A.

; SOUZA, ALESSANDRA M.T. DE

; BARROS, RITA C.

; CARMO, FLÁVIA A. DO

; ABREU, LETÍCIA C.L. DE

; MOREIRA, RONYSON S.S.

; HONÓRIO, THIAGO S.

; RODRIGUES, CARLOS R.

; SOUSA, VALERIA P. DE

; CABRAL, LUCIO M.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 2 elocation e20191445

Abstract: En | Text: En | PDF: En

Abstract Sildenafil is a potent selective inhibitor of phosphosdiesterase-5 previously used in erectile dysfunction and subsequently approved in 2005 for pulmonary arterial hypertension treatment. Since oral administration of sildenafil shows pharmacokinetic problems with mean absolute bioavailability of 41%, the goal of this work was to develop a novel sildenafil self-emulsifying drug delivery system (SEDDS) for oral absorption improvement and management of dosage. One pharmaceutical solution and four SEDDS containing sildenafil were successfully obtained and SEDDS formed O/W nanoemulsion with droplet size less than 300 nm. The stability studies evidenced that the SEDDS containing 3.3% w/w of sildenafil yielded the best results. The safety of 2-pyrrolidone/isobutanol in oral formulations was assessed in mice and no lethality was achieved in the placebo groups with LD50 of 490 mg/Kg for SEDDS II-3.3, suggesting it as a safe excipient for humans. Therewithal, in silico studies using PBPK models provided the pharmacokinetic profile of sildenafil SEDDS. Subsequently, in silico evaluation indicated that the sildenafil SEDDS droplet size influenced its bioavailability, enhancing absorption, assuring a good pharmacokinetic profile. These findings suggest that the formulations developed here presented the potential to enhance drug oral absorption with the possibility to control drug dosage as they are liquid pharmaceutical formulations.

https://doi.org/10.1590/0001-3765202020191445 1063 downloads

13.

Case report of staghorn calculi in a pediatric patient
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Silva, Isadora E.

; Abreu, Gabriela C.

; Moura, Wagner M.

; Cerqueira, Débora C.

; Leandro, Cinthia A. C.

Jornal Brasileiro de Patologia e Medicina Laboratorial

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jun 2019, Volume 55 N. 3 Pages 295 - 304

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Este relato ilustra um caso de cálculo do tipo coraliforme de grande volume, assintomático, acompanhado de uma revisão dessa grave patologia. Relatamos o caso de um paciente do sexo masculino, dois anos de idade, diagnosticado com cálculo coraliforme e hidronefrose em rim esquerdo. Instituiu-se terapêutica cirúrgica, com realização de pielolitotomia, pieloplastia e colocação de cateter duplo-J sem intercorrências. Cálculo renal coraliforme ocupa a pelve e os cálices renais. Sua incidência é menor em crianças, sendo a média de diagnóstico entre 7 e 10 anos. A etiologia é ampla; em menores de 4 anos, relaciona-se especialmente com fatores infecciosos e hidronefrose. O tratamento padrão é a nefrolitotomia percutânea.

ABSTRACT This article reports a case of large volume asymptomatic staghorn calculus, and a brief revision of this rare pathology is done. A two-yearold male patient was diagnosed with staghorn calculus and hydronephrosis in the left kidney. The patient underwent surgical therapy with pyelolithotomy, pyeloplasty and placement of a double-J catheter, without complications. Staghorn calculi occupy the pelvis and renal calyces. Their incidence in children is lower than in adults. The average age at diagnosis is between 7 and 10 years. The etiology for calculi is diverse; in children under 4 years, it is especially related with infectious factors and hydronephrosis. The standard procedure for treatment is percutaneous nephrolithotomy.

RESUMEN Presentamos un caso de cálculo del tipo coraliforme de gran volumen, asintomático, y una revisión de esa enfermedad grave. Reportamos el caso de un paciente masculino, de dos años de edad, diagnosticado con cálculo coraliforme e hidronefrosis en riñón izquierdo. Se realizó tratamiento quirúrgico, a base de pielolitotomía, pieloplastia y colocación de un catéter doble J, sin complicaciones. El cálculo coraliforme ocupa la pelvis y los cálices renales. Su incidencia es menor en niños, con media de 7-10 años en el diagnóstico. La etiología es diversa; en menores de 4 años, se refiere específicamente a factores infecciosos e hidronefrosis. El tratamiento estándar es la nefrolitotomía percutánea.

https://doi.org/10.5935/1676-2444.20190022 7818 downloads

14.

Epidemiological aspects of human and canine visceral leishmaniasis in State of Alagoas, Northeast, Brazil
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Rocha, M. A. N.

; Matos-Rocha, T. J.

; Ribeiro, C. M. B.

; Abreu, S. R. O.

Brazilian Journal of Biology

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nov 2018, Volume 78 N. 4 Pages 609 - 614

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Leishmaniose visceral zoonótica, causada por protozoários do gênero Leishmania, é uma doença importante no mundo. Na região nordeste do Brasil, do estado de Alagoas é endêmico para LVZ. Neste sentido, este trabalho teve como objetivo analisar a situação epidemiológica da leishmaniose visceral humana e canina em Alagoas, Nordeste, Brasil, no período de 2007 a 2013. Foi realizado um estudo descritivo, observacional, retrospectivo, usando-se secundário do Sistema de Informação de Agravos de Notificação (SINAN), Centro de Controle de Zoonoses de Maceió (CCZ) e Laboratório Central de Saúde Pública de Alagoas (LACEN/AL). Durante o período de estudo, observou-se que o ano de maior incidência de Leishmaniose visceral humana (LVH) foi o de 2011 e o de menor foi no ano de 2013. Já a LVC teve maior incidência em 2007 e menor em 2012. Dos 55 municípios do Estado de Alagoas que apresentaram LVH, São José da Tapera apresentou uma média de casos de 4,4 nos últimos cinco anos classificado como de transmissão intensa; No que diz respeito à leishmaniose visceral canina (LVC), no mesmo período de estudo, foram examinados 45.112 cães no Estado, dos quais 4.466 foram positivos. Resultou assim, em uma taxa de 9,9% de positividade. Nossos dados são importantes porque a infecção canina é um importante fator de risco para a doença humana.

Abstract Zoonotic visceral leishmaniasis (ZVL), caused by protozoans of the genus Leishmania, it is a worldwide of great importance disease. In the northeast region of Brazil, the state of Alagoas has an endemic status for ZVL. Thus, this work aimed to analyze the epidemiological situation of human and canine visceral leishmaniasis in Alagoas, Northeast, Brazil, from 2007 to 2013. We conducted a descriptive, observational, retrospective study using secondary data from the Notifiable Diseases Information System, the Center of Zoonosis Control of Maceió, and the Central Laboratory of Public Health of Alagoas. During the studied period, it was observed that the highest incidence of human visceral leishmaniasis was in 2011 and the lowest in 2013. On the other hand, canine visceral leishmaniasis had its highest incidence in 2007 and its lowest in 2012. Of the 55 municipalities in the State of Alagoas that showed human visceral leishmaniasis (HVL), São José da Tapera presented an average of 4.4 cases over the past five years, being classified as of intense transmission. Regarding canine visceral leishmaniasis, in the same studied period, 45,112 dogs were examined in the State, of which 4,466 were positive. It resulted, thus, in a 9.9% positivity rate. Conclusions: Our data are important because canine infection is an important risk factor for the human disease.

https://doi.org/10.1590/1519-6984.166622 3395 downloads

15.

Immunophenotyping lymphocyte and acute phase proteins in canine X-linked muscular dystrophy
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ABREU, DILAYLA K. DE

; MONTEIRO, JANAINA M.

; SOUZA, CAROLINA C.

; KARAM, RAFAEL G.

; FERNANDES, RENATA A.

; LESSA, THAIS B.

; FAGLIARI, JOSÉ J.

; MIGLINO, MARIA ANGELICA

; AMBROSIO, CARLOS EDUARDO

Anais da Academia Brasileira de Ciências

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sep 2018, Volume 90 N. 3 Pages 2977 - 2990

Abstract: En | Text: En | PDF: En

Abstract Duchenne Muscular Dystrophy (DMD) is the most common X-linked muscular disease affecting humans. The Golden Retriever Muscular Dystrophy model (GRMD) is considerthe most suitable for several studies. This assay aims to quantify lymphocyte subpopulations CD4, CD5, and CD8, and standardize, the serum electrophoretic profile, to understand their contribution to the pathologic process in normal Golden Retriever dogs (GR group) and dystrophic´s (GRMD group), through the umbilical cord blood, in dogs aged from 2 to 3 months (GR II and GRMD II), and in dogs over 1 year of age (GR III and GRMD III). No significant differences were observed between the CD8+ lymphocyte subpopulations of the groups studied. The CD4+ and CD5+ lymphocyte subpopulations were significantly higher in the GRMD III group compared to the GR III group. Twenty-two different proteins in the gel were identified. The serum concentrations of the proteins belonging to the GR I and GRMD I groups were significantly lower than those of the other groups. We show that expression of acute phase proteins are worst during the aging of the dogs. We hope to expand knowledge to better understand the GRMD model and the translational data.

https://doi.org/10.1590/0001-3765201820170838 1137 downloads

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