Comparative study of Cysticercus longicollis and Cysticercus cellulosae antigens in neurocysticercosis immunodiagnosis of human

COMPARATIVE STUDY OF Cysticercus longicollis AND Cysticercus cellulosae ANTIGENS IN NEUROCYSTICERCOSIS IMMUNODIAGNOSIS OF HUMAN (ABSTRACT)^*. DISSERTATION. CURITIBA, 2000.

RUBENS LUIZ FERREIRA GUSSO^**

Two immunodiagnosis tests were standardised: indirect fluorescent antibody test (IFAT) and enzyme¾linked immunosorbent assay (ELISA), for the detection of anti-Cysticercus cellulosae antibodies, in cerebrospinal fluid (CSF), in carriers of neurocysticercosis (with alive or degenerating cysticerci).

o antigens were studied: a homologous (Cysticercus cellulosae) and a heterologous (Cysticercus longicollis) antigen. For the production of Cysticercus longicollis antigen only the vesicular fluid was used on the ELISA test, which was much superior than Cysticercus cellulosae. For Cysticercus cellulosae, the parasitic membranes of the scolex and total saline extract were used due to the small yield of the vesicular liquid. For IFAT the number of particles was established in 20 to 30 (for field, increase of 400x). The conjugate concentration used was 1:250. The ELISA test was standardised: antigen concentration: 1mg/well; dilution of the CSF: 1/1; dilution of the conjugate: 1: 3000; cut-off to C. cellulosae was 0.210 and cut-off to C. longicollis was 0.306. When compared to the gold standard and the serologic techniques with homologous antigen, characterizing the definitive diagnosis, the IFAT sensitivity was of 90.6%, considering the total number of analyzed samples (39), being 90.5% for patients' samples with cysticerci in activity and 90.9% for patients' samples with calcifications in the encephalon. As for the ELISA test the sensitivity for the same group of samples was of 90.9%, being from 95.2% and 83.3%, for patients' samples with cysticerci in activity and for patients' samples with calcifications respectively, equally with definitive certainty diagnosis for neurocysticercosis.

The heterologous antigen can be used as alternative to the homologous antigen. The revenue and the control of the environmental variables in the maintenance of the stump and the indicators of the tests allow the use of this biological component for production of extracts destined antigens for the research of anti-Cysticercus cellulosae antibodies in CSF.

KEY WORDS: Cysticercuslongicollis; Cysticercuscellulosae; neurocysticercosis

**Address: Rua Cícero Jaime Brey 103, 82515-230 Curitiba PR, Brasil. E-mail: [e-mail]

THESES é seção de Arquivos de Neuro-Psiquiatria que divulga os resumos em inglês de teses defendidas e aprovadas. O assunto da tese deve estar relacionado a aspectos clínicos ou experimentais de interesse em neurologia, ciências a ela afins e psiquiatria.

Para publicação, o Autor deve encaminhar ao Editor: abstract da tese, acompanhado do título em inglês e em português; key words; disquete com a reprodução desses dados.

Adicionalmente, o Autor deve informar: a natureza da tese (Dissertação/Tese de Mestrado, Tese de Doutorado, Tese de Livre-Docência - como exemplos); nome da instituição na qual foi defendida e respectiva área de concentração; nome do Orientador, quando for o caso; endereço para correspondência.

*

Estudo comparativo dos antígenos de

Cysticercus longicollis e

Cysticercus cellulosae no imunodiagnóstico da neurocisticercose humana (Resumo). Dissertação de Mestrado, Universidade Federal do Paraná (Área:Ciências Veterinárias, Setor de Ciências Agrárias). Orientadora: Vanete Thomaz Soccol; co-orientador: Sérgio Monteiro de Almeida.

Publication Dates

• Publication in this collection
  10 Sept 2002
• Date of issue
  Sept 2002