Correlative scanning and transmission electron microscopic study on the ependymal surface of Cebus apella

Carvalho, Cáudio A. Ferraz de; Watanabe, II-Sei; Souza, Romeu Rodrigues de

doi:10.1590/S0004-282X1986000400003

Abstracts

Topographical variations of the ependymal surface of the whole brain ventricular system of Cebus apella, a wild Brazilian species, were studied at the scanning electron microscope after C02 critical point drying and carbon-gold coating. Certain selected areas were also studied at the transmission electron microscope, following standard technical procedures. We observed distinct patterns in the distribution of cilia, microvilli, other cell projections, and the so called supraependymal structures. Based on the morphological findings, functional considerations are made.

Variações topográficas da superfície ependimária de todo o sistema ventricular encefálico do Cebus apella, uma espécie silvestre brasileira, foram estudadas mediante microscopia eletrônica de varredura, após secagem pelo método do ponto crítico do C02 e metalização pelo ouro-carbono. Determinadas áreas foram também estudadas no microscópio eletrônico de transmissão, após os procedimentos técnicos usuais. Observamos distintos padrões na distribuição de cílios, microvilosidades, e outras projeções celulares, além das assim chamadas estruturas supraependimárias. São feitas considerações morfofuncionais, baseadas nos achados morfológicos.

Correlative scanning and transmission electron microscopic study on the ependymal surface of Cebus apella

Estudo correlativo à microscopia eletrônica de varredura e de transmissão da superfície do epêndima do Cebus apella

Cáudio A. Ferraz de Carvalho; II-Sei Watanabe; Romeu Rodrigues de Souza

Department of Anatomy, Biomedical Sciences Institute, São Paulo University

SUMMARY

Topographical variations of the ependymal surface of the whole brain ventricular system of Cebus apella, a wild Brazilian species, were studied at the scanning electron microscope after C02 critical point drying and carbon-gold coating. Certain selected areas were also studied at the transmission electron microscope, following standard technical procedures. We observed distinct patterns in the distribution of cilia, microvilli, other cell projections, and the so called supraependymal structures. Based on the morphological findings, functional considerations are made.

RESUMO

Variações topográficas da superfície ependimária de todo o sistema ventricular encefálico do Cebus apella, uma espécie silvestre brasileira, foram estudadas mediante microscopia eletrônica de varredura, após secagem pelo método do ponto crítico do C02 e metalização pelo ouro-carbono. Determinadas áreas foram também estudadas no microscópio eletrônico de transmissão, após os procedimentos técnicos usuais. Observamos distintos padrões na distribuição de cílios, microvilosidades, e outras projeções celulares, além das assim chamadas estruturas supraependimárias. São feitas considerações morfofuncionais, baseadas nos achados morfológicos.

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Departamento de Anatomia, Bloco-30, Instituto de Ciências Biomédicas, Universidade de São Paulo - Caixa Postal, 4365 - 01000 - São Paulo, SP - Brasil.

1. AGDUHR, E. - Ueber ein zentrales Sinneorgan (?) bei den Vertebraten. Z. anat. Entwicks-Gesch. 66:223, 1922.
2. BARGMANN, W. & SCHARRER, E. - The site of origin of the hormones of the posterior pituitary. Amer. Scientist 39:245, 1951.
3. DIERICKX, K. - The dendrites of the preoptic neurosecretory nucleus of Rana temporária and the osmoreceptors. Arch. int. Pharmacodyn. 140:708, 1962.
4. FERRAZ DE CARVALHO, CA.; COSTACURTA, L. & CARVALHO FILHO, J.R. - Histological and histochemical study on the ependyma of Bradypus tridactylus. Acta. anat. (Basel) 92-424, 1975.
5. FERRAZ DE CARVALHO, CA. & COSTACURTA, L. - (A) Ultrastructural study on topographical variations of the ependyma in Bradypus tridactylus. Acta. anat. (Basel) 94:369, 1976.
6. FERRAZ DE CARVALHO, CA.; KÖNIG JR., B. & RODRIGUES JR., A.J. - (B) Ultrastructural study on the relations among nerve elements and ependymal cells of the Bradypus tridactylus. Rev. bras. Pesq. méd. biol. 9:137, 1976.
7. FERRAZ DE CARVALHO, CA.; CHAGAS, CF.; SILVA, CF. & REIS, F.P. - Correlative scanning and transmission electron microscopic study on the ependymal surface of Bradypus tridactylus. Arq. Neuro-Psiquiat. (São Paulo) 41:64, 1983.
8. HETZEL, W. - Ependymal structure of the anterior and inferior horn of the lateral ventricle of the rabbit brain: scanning electr. Microsc. 2:129, 1978.
9. HETZEL, W. - The posterior horn and collateral trigone of the lateral ventricle of the monkey brain (Macaca speziona): a scanning electron microscopic study. Cell Tiss. Res. 186:161, 1978.
10. ITO, H. - The receotor in the reDtilian brain. J. Hirnforsch. 7:943, 1965.
11. KARNOVSKY, M.J. - A formaldehyde-gluteraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27:137, 1965.
12. LEONHARDT, H. - Intraventrikuläre markhaltige Nervenfasern nahe der Apertura lateralis ventriculi quarti des Kanninchengehirns. Z. Zellforsch. 84:1, 1968.
13. MARQUET, E.; SOBED, H. J.; SCHWARZ, E. & WEISS, M. - Secretion by ependymal cells of the neurohypophysis and saccus vasculosus of Polyypterus ornatipinnis (Osteichthyes). J. Morph. 137:111, 1972.
14. MITCHELL, J.A. - Scanning electron microscopy of brain ventricular surfaces: a bibliography. Scanning electr. Microsc. 3:475, 1980.
15. NAKAI, Y. - Fine structure and its functional properties of the ependymal cell in the frog median eminence. Z. Zellforsch, 122:15, 1971.
16. PENSA, A. - Delia esistenza di fibre nervose aventi speciali rapporti col ependyma. Boll. Soc. méd. chir. (Pavia), 1903. (Apud AGDUHR. E. 1922).
17. PESONEN, N. - Ueber die intraependymalen Nervenelemente. Anat. Anz. 90: 193, 1940.
18. PONTENAGEL, M. - Elektronenmikroskopische Untersuchungen am Ependym, der Plexus chorioidei bei Rana esculenta und Rana fusca (Roesel). Z. mik. anat. Forsch. 68:371, 1962.
19. RODRIGUES, E.M. - Neurosecretory system of the toad Bufo arenarum Hensel and its changes during inanition. Gen. comp. Endocr. 4:684, 1964.
20. SCHECHTER, J. & WEINER, R. - Ultrastructural changes in the ependymal lining of the median eminence following the intraventricular administration of catecholamine. Anat. Ree. 172:643, 1972.
21. TAKEICHI, M. - The fine structure of ependymal cells. II. An electron microscopic study of the soft-shelled turtle paraventricular organ, with special reference to the first structure of ependymal cells and so called albuminous substance. Z. Zellforsch. 76:471, 1967.
22. WEINDL, A. & SCHINKO, I. - The ventricular system of the opossum brain. Scanning electr. Microsc. 2:861, 1978.

Publication Dates

Publication in this collection
29 June 2011
Date of issue
Dec 1986

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AGDUHR, E. - Ueber ein zentrales Sinneorgan (?) bei den Vertebraten. Z. anat. Entwicks-Gesch. 66:223, 1922.

[2] 2. BARGMANN, W. & SCHARRER, E. - The site of origin of the hormones of the posterior pituitary. Amer. Scientist 39:245, 1951.

[3] 3. DIERICKX, K. - The dendrites of the preoptic neurosecretory nucleus of Rana temporária and the osmoreceptors. Arch. int. Pharmacodyn. 140:708, 1962.

[4] 4. FERRAZ DE CARVALHO, CA.; COSTACURTA, L. & CARVALHO FILHO, J.R. - Histological and histochemical study on the ependyma of Bradypus tridactylus. Acta. anat. (Basel) 92-424, 1975.

[5] 5. FERRAZ DE CARVALHO, CA. & COSTACURTA, L. - (A) Ultrastructural study on topographical variations of the ependyma in Bradypus tridactylus. Acta. anat. (Basel) 94:369, 1976.

[6] 6. FERRAZ DE CARVALHO, CA.; KÖNIG JR., B. & RODRIGUES JR., A.J. - (B) Ultrastructural study on the relations among nerve elements and ependymal cells of the Bradypus tridactylus. Rev. bras. Pesq. méd. biol. 9:137, 1976.

[7] 7. FERRAZ DE CARVALHO, CA.; CHAGAS, CF.; SILVA, CF. & REIS, F.P. - Correlative scanning and transmission electron microscopic study on the ependymal surface of Bradypus tridactylus. Arq. Neuro-Psiquiat. (São Paulo) 41:64, 1983.

[8] 8. HETZEL, W. - Ependymal structure of the anterior and inferior horn of the lateral ventricle of the rabbit brain: scanning electr. Microsc. 2:129, 1978.

[9] 9. HETZEL, W. - The posterior horn and collateral trigone of the lateral ventricle of the monkey brain (Macaca speziona): a scanning electron microscopic study. Cell Tiss. Res. 186:161, 1978.

[10] 10. ITO, H. - The receotor in the reDtilian brain. J. Hirnforsch. 7:943, 1965.

[11] 11. KARNOVSKY, M.J. - A formaldehyde-gluteraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27:137, 1965.

[12] 12. LEONHARDT, H. - Intraventrikuläre markhaltige Nervenfasern nahe der Apertura lateralis ventriculi quarti des Kanninchengehirns. Z. Zellforsch. 84:1, 1968.

[13] 13. MARQUET, E.; SOBED, H. J.; SCHWARZ, E. & WEISS, M. - Secretion by ependymal cells of the neurohypophysis and saccus vasculosus of Polyypterus ornatipinnis (Osteichthyes). J. Morph. 137:111, 1972.

[14] 14. MITCHELL, J.A. - Scanning electron microscopy of brain ventricular surfaces: a bibliography. Scanning electr. Microsc. 3:475, 1980.

[15] 15. NAKAI, Y. - Fine structure and its functional properties of the ependymal cell in the frog median eminence. Z. Zellforsch, 122:15, 1971.

[16] 16. PENSA, A. - Delia esistenza di fibre nervose aventi speciali rapporti col ependyma. Boll. Soc. méd. chir. (Pavia), 1903. (Apud AGDUHR. E. 1922).

[17] 17. PESONEN, N. - Ueber die intraependymalen Nervenelemente. Anat. Anz. 90: 193, 1940.

[18] 18. PONTENAGEL, M. - Elektronenmikroskopische Untersuchungen am Ependym, der Plexus chorioidei bei Rana esculenta und Rana fusca (Roesel). Z. mik. anat. Forsch. 68:371, 1962.

[19] 19. RODRIGUES, E.M. - Neurosecretory system of the toad Bufo arenarum Hensel and its changes during inanition. Gen. comp. Endocr. 4:684, 1964.

[20] 20. SCHECHTER, J. & WEINER, R. - Ultrastructural changes in the ependymal lining of the median eminence following the intraventricular administration of catecholamine. Anat. Ree. 172:643, 1972.

[21] 21. TAKEICHI, M. - The fine structure of ependymal cells. II. An electron microscopic study of the soft-shelled turtle paraventricular organ, with special reference to the first structure of ependymal cells and so called albuminous substance. Z. Zellforsch. 76:471, 1967.

[22] 22. WEINDL, A. & SCHINKO, I. - The ventricular system of the opossum brain. Scanning electr. Microsc. 2:861, 1978.

Brasil

Brasil

Correlative scanning and transmission electron microscopic study on the ependymal surface of Cebus apella

Estudo correlativo à microscopia eletrônica de varredura e de transmissão da superfície do epêndima do Cebus apella

Abstracts

Publication Dates