Notes on electropherograms of eye-lens, muscle proteins and zymograms of muscle esterases of fish collected during the first Brazilian expedition to the Antarctica

Ngan, Phan Van; Suzuki, Hana; Gomes, Vicente; Passos, Maria José de Arruda Campos Rocha

doi:10.1590/S0373-55241985000200008

Abstracts

A preliminary study was carried out on electropherograms of eye-lens, muscle proteins and zymograms of muscle esterases of ten Notothenia larseni, six Notothenia nudifrons and one lanternfish, Electrona antarctica. The fish were collected by the R/V "Prof. W. Besnard" of the Institute of Oceanography, University of São Paulo, during the First Brazilian Expedition to Antarctica. Eye-lens proteins were analysed on cellulose acetate membrane, muscle proteins and esterases on gel of polyaorylamide. Eye-lens proteins showed three types of electropherograms for N. larseni, and two types for N. nudifrons. One of the electropherograms of N. larseni can be readily distinguished from those of N. nudifrons. Electropherograms of muscle proteins of N. larseni and N. nudifrons are very similar and, consist of sixteen to seventeen fractions. Electropherograms of muscle proteins of N. larseni are severely affected by the conservation of the extracts overnight under -20ºC. All N. nudifrons were of the same zymograms of esterases while those of N. larseni varied. Electropherograms of eye-lens and muscle proteins as well as zymograms of esterases of the lanternfish are different from those of nototheniids.

Marine fishes; Electrophoresis; Esterases; Proteins; Muscle; Eye-lens; Notothenia larseni; Notothenia nudifrons; Electrona antarctica; R/V "Prof. W. Besnard"; Bransfield Strait; Antarctica

Foi realizado um estudo preliminar sobre eletroferogramas de proteínas de cristalino e de músculo esquelético, e zimogramas de esterases de músculo esquelético de dez Notothenia larseni, seis Notothenia nudifrons e de um peixe-lanterna, Electrona antarctica. Os peixes foram coletados pelo N/Oc. "Prof. W. Besnard" do Instituto Oceanográfico da Universidade de São Paulo durante a I Expedição Brasileira à Antártica. As proteinas do cristalino foram analisadas em membranas de acetato de celulose, enquanto que as proteínas e esterases do músculo esquelético, em gel de poliacrilamida. As proteínas do cristalino apresentam três tipos distintos de eletroferogramas para N. larseni, e dois para N. nudifrons. Um dos eletroferogramas de N. larseni, pode ser prontamente distinguido dos de N. nudifrons. Eletrof erogramas de proteínas de músculo de N. larseni e de N. nudifrons são muito semelhantes e consistem de 16 a 17 frações. Os eletroferogramas de proteínas de músculo de N. larseni são severamente afetados pela preservação dos extratos por uma noite a -20ºC. Todos os N. nudifrons apresentam um mesmo zimograma de esterases enquanto que os de N. larseni variam. Tanto os eletroferogramas de proteínas do cristalino e do músculo como os zimogramas de esterases do peixe-lanterna são diferentes dos apresentados pelos nototeniideos.

Peixes marinhos; Eletroforese; Esterases; Proteínas; Músculo; Cristalino; Notothenia larseni; Notothenia nudifrons; Electrona antarctica; N/Oc. "Prof. W. Besnard"; Estreito de Bransfield; Antártica

ARTIGOS

Notes on electropherograms of eye-lens, muscle proteins and zymograms of muscle esterases of fish collected during the first Brazilian expedition to the Antarctica

Phan Van Ngan; Hana Suzuki^* (* ) Graduate Student, Institute of Oceanography, Departament of Biological Oceanography, Fellow of CNPq. ; Vicente Gomes^* (* ) Graduate Student, Institute of Oceanography, Departament of Biological Oceanography, Fellow of CNPq. ; Maria José de Arruda Campos Rocha Passos^** (** ) CIRM - IOUSP

Instituto Oceanográfico da Universidade de São Paulo (Caixa Postal, 9075, 01000 São Paulo, SP)

SYNOPSIS

A preliminary study was carried out on electropherograms of eye-lens, muscle proteins and zymograms of muscle esterases of ten Notothenia larseni, six Notothenia nudifrons and one lanternfish, Electrona antarctica. The fish were collected by the R/V "Prof. W. Besnard" of the Institute of Oceanography, University of São Paulo, during the First Brazilian Expedition to Antarctica. Eye-lens proteins were analysed on cellulose acetate membrane, muscle proteins and esterases on gel of polyaorylamide. Eye-lens proteins showed three types of electropherograms for N. larseni, and two types for N. nudifrons. One of the electropherograms of N. larseni can be readily distinguished from those of N. nudifrons. Electropherograms of muscle proteins of N. larseni and N. nudifrons are very similar and, consist of sixteen to seventeen fractions. Electropherograms of muscle proteins of N. larseni are severely affected by the conservation of the extracts overnight under -20ºC. All N. nudifrons were of the same zymograms of esterases while those of N. larseni varied. Electropherograms of eye-lens and muscle proteins as well as zymograms of esterases of the lanternfish are different from those of nototheniids.

Descriptors: Marine fishes, Electrophoresis, Esterases, Proteins, Muscle, Eye-lens, Notothenia larseni, Notothenia nudifrons, Electrona antarctica, R/V "Prof. W. Besnard", Bransfield Strait, Antarctica.

RESUMO

Foi realizado um estudo preliminar sobre eletroferogramas de proteínas de cristalino e de músculo esquelético, e zimogramas de esterases de músculo esquelético de dez Notothenia larseni, seis Notothenia nudifrons e de um peixe-lanterna, Electrona antarctica. Os peixes foram coletados pelo N/Oc. "Prof. W. Besnard" do Instituto Oceanográfico da Universidade de São Paulo durante a I Expedição Brasileira à Antártica. As proteinas do cristalino foram analisadas em membranas de acetato de celulose, enquanto que as proteínas e esterases do músculo esquelético, em gel de poliacrilamida.

As proteínas do cristalino apresentam três tipos distintos de eletroferogramas para N. larseni, e dois para N. nudifrons. Um dos eletroferogramas de N. larseni, pode ser prontamente distinguido dos de N. nudifrons. Eletrof erogramas de proteínas de músculo de N. larseni e de N. nudifrons são muito semelhantes e consistem de 16 a 17 frações. Os eletroferogramas de proteínas de músculo de N. larseni são severamente afetados pela preservação dos extratos por uma noite a -20ºC.

Todos os N. nudifrons apresentam um mesmo zimograma de esterases enquanto que os de N. larseni variam.

Tanto os eletroferogramas de proteínas do cristalino e do músculo como os zimogramas de esterases do peixe-lanterna são diferentes dos apresentados pelos nototeniideos.

Descritores: Peixes marinhos, Eletroforese, Esterases, Proteínas, Músculo, Cristalino, Notothenia larseni, Notothenia nudifrons, Electrona antarctica, N/Oc. "Prof. W. Besnard", Estreito de Bransfield, Antártica.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Acknowledgements

The authors wish to express their thanks to Dr Adolf Kellermann, Institut fur Polarökologie der Christian-Albrechts Universität, and Mr. Uwe Rowedder, Küsten und Binnenfischerei, Kiel, West Germany, for identification of the nototheniids and the lanternfish, respectively. The authors wish also to thank Dr Renato Amaral, Universidade Federal do Rio Grande do Sul, Brazil, for his excellent assistance in sampling during the Expedition, and to Mrs Maria Cecilia Catunda for polishing the English in the manuscript.

(Received 03-Nov-83; accepted 17-Dec-85)

AKROYD, P. 1967. Acrylamide gel slab: electrophoresis in a simple glass cell for improved resolution and comparison of serum protein. Analytical Biochem., 19:399-410.
AYALA, F. J., ed. 1976. Molecular evolution. Sunderland, Mass., Sinauer Associates, 277p.
BARRETT, I. & WILLIAMS, A. A. 1967. Soluble lens proteins of some scombroid fishes. Copeia, (2):468-471.
BON, W. F. 1957. Some physico-chemical data about the ontogenetic and phylogenetic development of the eye lens proteins of fish. Pubbl. Staz. Zool. Napoli, 30 (3):373-380.
BREWER, J. M.; PESCE, A. J. & ASHWORTH, R. B. 1974. Experimental techniques in biochemistry. New Jersey, Prentice-Hall, p.351.
De LIGNY, W. 1969. Serological and biochemical studies on fish populations. Oceanogr. Mar. Biol. Ann. Rev., 7:411-513.
______ 1972. Blood and biochemical polymorphism in fishes. In: European Conference on Animal Blood Groups and Biochemical Polymorphisms:12. The Hague, Dr. W. Junk, p.55-65.
FLOWERDEW, M. W. & CRISP, D. J. 1975. Esterase heterogeneity and an investigation into racial differences in the cirriped Balanus balanoides using acrylamide gel electrophoresis. Mar. Biol., 33:33-39.
GORNALL, A. C.; BARDAWILL, C. S. & DAVID, M. M. 1949. Determination of serum proteins by means of biuret reaction. J. biol. Chem., 177:751-766.
HAEN, P. J. & O'ROURKE, F. J. 1969. Comparative electrophoretic studies on soluble eyelens proteins of some Irish freshwater fishes. Proc. R. Ir. Acad., 68(B4):67-75.
HUNTER, R. L. & MARKERT, C. L. 1957. Histochemical demonstration of enzymes separated by zone electrophoresis in starch gel. Science, N.Y., 125:1294-1295.
MOORE, G. S.; PETERS, H. A. & LEVIN, R. E. 1970. Alterations in the electrophoretic patterns of refrigerated fish. J. Fish. Res. Bd Can., 27:31-38.
O'ROURKE, F. J. 1974. Fish. In: Wright, C. A., ed. - Biochemical and immunological taxonomy of animals. London, Academic Press, p.243-302.
PETERSON, G. L. & SHEHADEH, Z. H. 1971. Subpopulations of the Hawaiian striped mullet Mugil cephalus: analysis of variations of nuclear eye-lens protein electropherograms and nuclear eye-lens weights. Mar. Biol., 11( 1):52-60.
PHAN, V. N. 1980. Estudo bioquímico e fisiológico sobre os bagres marinhos do Brasil. I. Sobre padrão eletroforético do plasma em gel de poliacrilamida dos bagres da região estuarino lagunar de Cananéia. Bolm Inst. oceanogr., S Paulo, 29(2):301-303.
______ ; VAZZOLER, A. E. A. de M. & PARDO, W. M. 1977. Micropogon furnieri II. Estudo dos padrões eletroforéticos de proteínas totais de cristalinos da população I (Cabo Frio - Torres). Ciênc. Cult., S Paulo, 29(7, supl.):539. Abstract.
SCOPES, R. K. 1968. Methods for starch gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscle of a variety of species. Biochem J., 107:139-150.
SELANDER, R. K. & JOHNSON, W. E. 1973. Genetic variation among vertebrate species. Ann. Rev. Ecol. Syst., 4: 75-91.
SMITHIES, O. 1959. An improved procedure for starch-gel electrophoresis: further variation in the serum proteins of normal adults. Biochem. J., 71:585-587.
SUZUKI, H.; VAZZOLER, A. E. A. de M. & PHAN, V. N. 1983. Estudo eletroforético de proteínas do músculo esquelético de Micropogonias furnieri (Desmarest, 1822) da costa SE-S do Brasil. I. Considerações técnicas. Bolm Inst. oceanogr., S Paulo, 32(2): 153-165.
TSUYUKI, H.; ROBERTS, E. & VANSTONE, W. E. 1965. Comparative zone electropherograms of muscle myogens and blood hemoglobins of marine and freshwater vertebrates and their application to biochemical systematics. J. Fish. Res. Bd Can., 22(1):203-213.
VAZZOLER, A. E. A. de M. & PHAN, V. N. 1981. Ocorrência de "catarata em Micropogonias furnieri (Desmarest, 1822), na área entre Cabo Frio e Torres (23şS - 29şS), Brasil: investigação de causas e estudo eletroforético das proteínas totais dos cristalinos. Bolm Inst. oceanogr., S Paulo, 30(1):65-76.
ZIGMAN, S. & YULO, T. 1979. Eye lens ageing in the dogfish (Mustelus canis). Comp. Biochem. Physiol., 63B:379-385.

(*

) Graduate Student, Institute of Oceanography, Departament of Biological Oceanography, Fellow of CNPq.

(**

) CIRM - IOUSP

Publication Dates

Publication in this collection
04 June 2012
Date of issue
1985

History

Accepted
17 Dec 1985
Received
03 Nov 1983

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] AKROYD, P. 1967. Acrylamide gel slab: electrophoresis in a simple glass cell for improved resolution and comparison of serum protein. Analytical Biochem., 19:399-410.

[2] AYALA, F. J., ed. 1976. Molecular evolution. Sunderland, Mass., Sinauer Associates, 277p.

[3] BARRETT, I. & WILLIAMS, A. A. 1967. Soluble lens proteins of some scombroid fishes. Copeia, (2):468-471.

[4] BON, W. F. 1957. Some physico-chemical data about the ontogenetic and phylogenetic development of the eye lens proteins of fish. Pubbl. Staz. Zool. Napoli, 30 (3):373-380.

[5] BREWER, J. M.; PESCE, A. J. & ASHWORTH, R. B. 1974. Experimental techniques in biochemistry. New Jersey, Prentice-Hall, p.351.

[6] De LIGNY, W. 1969. Serological and biochemical studies on fish populations. Oceanogr. Mar. Biol. Ann. Rev., 7:411-513.

[7] ______ 1972. Blood and biochemical polymorphism in fishes. In: European Conference on Animal Blood Groups and Biochemical Polymorphisms:12. The Hague, Dr. W. Junk, p.55-65.

[8] FLOWERDEW, M. W. & CRISP, D. J. 1975. Esterase heterogeneity and an investigation into racial differences in the cirriped Balanus balanoides using acrylamide gel electrophoresis. Mar. Biol., 33:33-39.

[9] GORNALL, A. C.; BARDAWILL, C. S. & DAVID, M. M. 1949. Determination of serum proteins by means of biuret reaction. J. biol. Chem., 177:751-766.

[10] HAEN, P. J. & O'ROURKE, F. J. 1969. Comparative electrophoretic studies on soluble eyelens proteins of some Irish freshwater fishes. Proc. R. Ir. Acad., 68(B4):67-75.

[11] HUNTER, R. L. & MARKERT, C. L. 1957. Histochemical demonstration of enzymes separated by zone electrophoresis in starch gel. Science, N.Y., 125:1294-1295.

[12] MOORE, G. S.; PETERS, H. A. & LEVIN, R. E. 1970. Alterations in the electrophoretic patterns of refrigerated fish. J. Fish. Res. Bd Can., 27:31-38.

[13] O'ROURKE, F. J. 1974. Fish. In: Wright, C. A., ed. - Biochemical and immunological taxonomy of animals. London, Academic Press, p.243-302.

[14] PETERSON, G. L. & SHEHADEH, Z. H. 1971. Subpopulations of the Hawaiian striped mullet Mugil cephalus: analysis of variations of nuclear eye-lens protein electropherograms and nuclear eye-lens weights. Mar. Biol., 11( 1):52-60.

[15] PHAN, V. N. 1980. Estudo bioquímico e fisiológico sobre os bagres marinhos do Brasil. I. Sobre padrão eletroforético do plasma em gel de poliacrilamida dos bagres da região estuarino lagunar de Cananéia. Bolm Inst. oceanogr., S Paulo, 29(2):301-303.

[16] ______ ; VAZZOLER, A. E. A. de M. & PARDO, W. M. 1977. Micropogon furnieri II. Estudo dos padrões eletroforéticos de proteínas totais de cristalinos da população I (Cabo Frio - Torres). Ciênc. Cult., S Paulo, 29(7, supl.):539. Abstract.

[17] SCOPES, R. K. 1968. Methods for starch gel electrophoresis of sarcoplasmic proteins. An investigation of the relative mobilities of the glycolytic enzymes from the muscle of a variety of species. Biochem J., 107:139-150.

[18] SELANDER, R. K. & JOHNSON, W. E. 1973. Genetic variation among vertebrate species. Ann. Rev. Ecol. Syst., 4: 75-91.

[19] SMITHIES, O. 1959. An improved procedure for starch-gel electrophoresis: further variation in the serum proteins of normal adults. Biochem. J., 71:585-587.

[20] SUZUKI, H.; VAZZOLER, A. E. A. de M. & PHAN, V. N. 1983. Estudo eletroforético de proteínas do músculo esquelético de Micropogonias furnieri (Desmarest, 1822) da costa SE-S do Brasil. I. Considerações técnicas. Bolm Inst. oceanogr., S Paulo, 32(2): 153-165.

[21] TSUYUKI, H.; ROBERTS, E. & VANSTONE, W. E. 1965. Comparative zone electropherograms of muscle myogens and blood hemoglobins of marine and freshwater vertebrates and their application to biochemical systematics. J. Fish. Res. Bd Can., 22(1):203-213.

[22] VAZZOLER, A. E. A. de M. & PHAN, V. N. 1981. Ocorrência de "catarata em Micropogonias furnieri (Desmarest, 1822), na área entre Cabo Frio e Torres (23şS - 29şS), Brasil: investigação de causas e estudo eletroforético das proteínas totais dos cristalinos. Bolm Inst. oceanogr., S Paulo, 30(1):65-76.

[23] ZIGMAN, S. & YULO, T. 1979. Eye lens ageing in the dogfish (Mustelus canis). Comp. Biochem. Physiol., 63B:379-385.