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Brazilian Archives of Biology and Technology
On-line version ISSN 1678-4324
QUERIDO, André Luiz de Souza; COELHO, Jorge Luiz Cavalcante; ARAUJO, Elza Fernandes de and CHAVES-ALVES, Virgínia Maria. Partial purification and characterization of xylanase produced by Penicillium expansum. Braz. arch. biol. technol. [online]. 2006, vol.49, n.3, pp.475-480. ISSN 1678-4324. http://dx.doi.org/10.1590/S1516-89132006000400016.
An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mmol min-1 mg -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+.
Keywords : Partial purification; xylan [xylanases]; Penicillium expansum.