Cutaneous mast cell tumor in a captive Bush dog (Speothos venaticus): pathological and immunophenotypical aspects - case report

A senile male captive bush dog (Speothos venaticus) presented a small perianal cutaneous nodule. Histologically, there was an ulcerated round cell tumor composed of well differentiated mast cells with abundant intracytoplasmic purple Giemsa-positive granules, with a diffuse eosinophilic infiltrate. Immunohistochemistry revealed that 30% of the neoplastic cells were positive for Kit in the cytoplasm and cell membrane, and all neoplastic cells were negative for MAC and CD3. Less than 10% of the neoplastic cells were positive for Ki67. At necropsy other primary tumors were identified in this animal, including an intestinal adenoma, an adrenal cortex adenoma and a testicular interstitial cell tumor.

Keywords:
wild canids; immunohistochemistry; multiple tumors

Authorship

D.O. Santos

Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, BrasilUniversidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte, MG, Brasil

http://orcid.org/0000-0002-3083-1572

A.R. Oliveira

http://orcid.org/0000-0002-9031-792X

T.P. Carvalho

http://orcid.org/0000-0002-7158-8249

N.F. Paula

http://orcid.org/0000-0002-2506-1163

H.P. Tinoco

Fundação de Parques Municipais e Zoobotânica, Jardim Zoológico, Belo Horizonte, MG, BrasilFundação de Parques Municipais e ZoobotânicaBrasilBelo Horizonte, MG, BrasilFundação de Parques Municipais e Zoobotânica, Jardim Zoológico, Belo Horizonte, MG, Brasil

http://orcid.org/0000-0003-4270-5503

C.M. Coelho

http://orcid.org/0000-0002-5689-6198

A.T.P.G. Ribeiro

http://orcid.org/0000-0003-0339-1767

G.C. Martins

Centrovet, Belo Horizonte, MG, BrasilCentrovetBrasilBelo Horizonte, MG, BrasilCentrovet, Belo Horizonte, MG, Brasil

http://orcid.org/0000-0002-4317-4233

E.S. Veloso

Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, MG, BrasilUniversidade Federal de Minas GeraisBrazilBelo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Belo Horizonte, MG, Brasil

http://orcid.org/0000-0003-4696-7738

E. Ferreira

http://orcid.org/0000-0002-1835-0303

F. Pierezan

http://orcid.org/0000-0002-3319-8784

R.L. Santos ^**Corresponding author: rsantos@vet.ufmg.br

http://orcid.org/0000-0002-4830-0470

*Corresponding author: rsantos@vet.ufmg.br

SCIMAGO INSTITUTIONS RANKINGS

Centrovet, Belo Horizonte, MG, BrasilCentrovetBrasilBelo Horizonte, MG, BrasilCentrovet, Belo Horizonte, MG, Brasil

Figures | Tables

Figures (3)
Tables (2)

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Figure 1
Cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Ulcerated, infiltrative, poorly demarcated, and non-encapsulated round cell neoplasm confined to superficial dermis, HE, 50x. b. Well differentiated neoplastic mast cells with mild nuclear and cellular pleomorphism and mitotic figures (arrows). Marked infiltration of eosinophils. HE, 400x. c. Moderate number of purple granules at the cytoplasm of the neoplastic cell, characterizing the mast cell origin, Giemsa, 400x.

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Figure 2
Immunohistochemistry of a cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Mild immunolabeling of KIT protein, observed both at cell membrane and cytoplasm, from > 30% neoplastic cells, DAB, 200x. b. Proliferation index using Ki67 with nuclear immunolabeling in < 10% of neoplastic cells, DAB, 200x. c. Higher magnification of a, DAB, 1000x. d. Higher magnification of b, DAB, 1000x.

Thumbnail

Figure 3
Intestinal adenoma, adrenal cortex adenoma and testicular interstitial cell tumor in a bush dog (Speothos venaticus). a,b. Intestinal adenoma. a. Intestinal mucosa with a polypoid proliferation projecting into the lumen. HE, 50x. b. Well differentiated epithelial cells organized in tubules with abundant goblet cells. HE, 100x. c,d. Adrenal cortex adenoma. c. Cortical expansive neoplasm organized in nests separated by connective tissue and with multifocal areas of mineralization. HE, 50x. d. Well differentiated neoplastic cells with minimal cellular and nuclear pleomorphism and vacuolated cytoplasm. HE, 200x. e. Testicular interstitial cell tumor. Well differentiated interstitial neoplastic cells with vacuolated cytoplasm between seminiferous tubules. HE, 200x.

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Table 1
Immunohistochemistry protocol for detection of MAC, CD3, c-KIT, and Ki67*

Thumbnail

Table 2
Immunohistochemistry (IHC) results for MAC, CD3, KIT protein and Ki67 expression in the cutaneous mast cell tumor neoplastic cells in a bush dog (Speothos venaticus)

Figure 1 Cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Ulcerated, infiltrative, poorly demarcated, and non-encapsulated round cell neoplasm confined to superficial dermis, HE, 50x. b. Well differentiated neoplastic mast cells with mild nuclear and cellular pleomorphism and mitotic figures (arrows). Marked infiltration of eosinophils. HE, 400x. c. Moderate number of purple granules at the cytoplasm of the neoplastic cell, characterizing the mast cell origin, Giemsa, 400x.

Figure 2 Immunohistochemistry of a cutaneous mast cell tumor in a bush dog (Speothos venaticus). a. Mild immunolabeling of KIT protein, observed both at cell membrane and cytoplasm, from > 30% neoplastic cells, DAB, 200x. b. Proliferation index using Ki67 with nuclear immunolabeling in < 10% of neoplastic cells, DAB, 200x. c. Higher magnification of a, DAB, 1000x. d. Higher magnification of b, DAB, 1000x.

Figure 3 Intestinal adenoma, adrenal cortex adenoma and testicular interstitial cell tumor in a bush dog (Speothos venaticus). a,b. Intestinal adenoma. a. Intestinal mucosa with a polypoid proliferation projecting into the lumen. HE, 50x. b. Well differentiated epithelial cells organized in tubules with abundant goblet cells. HE, 100x. c,d. Adrenal cortex adenoma. c. Cortical expansive neoplasm organized in nests separated by connective tissue and with multifocal areas of mineralization. HE, 50x. d. Well differentiated neoplastic cells with minimal cellular and nuclear pleomorphism and vacuolated cytoplasm. HE, 200x. e. Testicular interstitial cell tumor. Well differentiated interstitial neoplastic cells with vacuolated cytoplasm between seminiferous tubules. HE, 200x.

Table 1 Immunohistochemistry protocol for detection of MAC, CD3, c-KIT, and Ki67*

Antibody	Manufacturer	Clone	Dilution	Incubation time (h)	Antigen retrieval**	Amplification
MAC387	AbdSerotec	MCA874G	1:400	16	Citratebuffer+Pascal	Novolink
CD3	Dako	Polyclonal	1:300	16	Citratebuffer+Pascal	Novolink
c-KIT	Dako	Polyclonal	1:800	16	Citratebuffer+Pascal	Novolink
Ki-67	Dako	MIB-1	1:50	16	Citratebuffer+Pascal	Novolink

*MAC: anti-macrophage; CD3: anti-T lymphocyte; c-KIT: anti-KIT; Ki-67: proliferation cell marker.
** Pascal - Pressurized humid heat (125(C).

Table 2 Immunohistochemistry (IHC) results for MAC, CD3, KIT protein and Ki67 expression in the cutaneous mast cell tumor neoplastic cells in a bush dog (Speothos venaticus)

IHC antibody	Results	Localization
MAC	Negative	-
CD3	Negative	-
KIT protein	> 30%	Membrane and cytoplasm
Ki67	< 10%	Nuclei

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Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

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Cutaneous mast cell tumor in a captive Bush dog (Speothos venaticus): pathological and immunophenotypical aspects - case report

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[1] *Corresponding author: rsantos@vet.ufmg.br