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Antifungal effect of propolis alcoholic extracts on grapevine pathogens

The objective of this work was to verify the in vitro effect of alcoholic extracts of propolis (AEP) on the germination of spores of Phakopsora euvitis and Pseudocercospora vitis, and also on the mycelial growth and sporulation of Elsinoe ampelina. For the germination assay the AEP was used at concentrations of 0.025; 0.05; 0.1; 0.2; 0.25 and 0.5%. Distilled water, ethanol at 0.35% and the fungicide azoxystrobin were used as control treatments. For mycelial growth and sporulation the concentrations 0.05; 0.1; 0.2; 0.4; 0.5 and 1.0% of AEP were used. The control treatments were potato-dextrose-agar (PDA) medium, PDA + 0.7% ethanol and the fungicide azoxystrobin. The results showed that AEP has a low in vitro antifungal activity against P. euvitis, P. vitis and E. ampelina, for the tested concentrations.

Phakopsora euvitis; Pseudocercospora vitis; Elsinoe ampelina

Instituto Biológico Av. Conselheiro Rodrigues Alves, 1252 - Vila Mariana - São Paulo - SP, 04014-002 - São Paulo - SP - Brazil