Effects of three <i>Bacillus</i> specious on hatchability, growth performance and serum biochemistry in Japanese quails fed diet contaminated with Aflatoxin B1

Mojgani, Naheed; Razmgah, Niloofar; Torshizi, Mohammad Amir Karimi; Sanjabi, Mohammad Reza

doi:10.4025/actascianimsci.v42i1.50184

Acessibilidade / Reportar erro

Brasil

Acta Scientiarum. Animal Sciences

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

NONRUMINANTS NUTRITION • Acta Sci., Anim. Sci. 42 • 2020 • https://doi.org/10.4025/actascianimsci.v42i1.50184 copy

Effects of three Bacillus specious on hatchability, growth performance and serum biochemistry in Japanese quails fed diet contaminated with Aflatoxin B1

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT.

In total, 240 one-day-old Japanese quails (Coturnix Coturnix Japonica) allocated at random to 6 treatments with 4 replicates and 10 birds in each. Treatments used were: 1) Negative control (without any additives or AFB1); 2) Positive control (basal diet + 2.5 ppm AFB1; 2); 3) TA008 (positive control + 10⁸ cfu/ml Bacillus. megaterium TA008); 4) TA049 (positive control + 10⁸ cfu mL^-1 Bacillus. subtilis TA049); 5) TA010 (positive control+ 10⁸ cfu mL^-1 Brevibacillus brevis TA010) and 6) P (positive control + 2.5 g kg^-1 Polysorb® in feed). Hatchability and embryonic mortality were significantly influenced by additives and AFB1 (p < 0.05). Birds fed TA008 improved 12 % hatchability and reduced 10 % embryonic mortality in compared to positive control (p < 0.05). Weight gain and feed conversion ratio did not affected by treatments (p > 0.05). Feed intake was significantly improved in birds feeding by TA008 at 0-21 days (p < 0.05). There were significant differences on relative weights of carcass, gizzard and proventriculus among treatments (p < 0.05). Serum total protein, albumin, cholesterol, glucose, HDL, globulin and uric acid were significantly affected by treatments (p < 0.05). These results showed that the inclusion of bacillus megaterium as potential probiotic into contaminated diets could improve the adverse effects of AFB1 in Japanese quails.

Keywords:
Bacillus. sp.; Aflatoxin B1; japanese quail; hatchability; productive performance.

Editora da Universidade Estadual de Maringá - EDUEM Av. Colombo, 5790, bloco 40, CEP 87020-900 , Tel. (55 44) 3011-4253, Fax (55 44) 3011-1392 - Maringá - PR - Brazil
E-mail: actaanim@uem.br

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Author for correspondence. E-mail: nmoj1398@gmail.com

Nutrients	%
Fat	7
Fiber	4
Methionine	0.46
Lysine	1.2
Methionine + Cysteine	1.09
Calcium	1.03
Available phosphorous	0.7
Metabolizable Energy (Kcal kg^-1)	2900
Crude protein	20

Index	Level
Protein %	Maximum 25
Beta-glucan %	Minimum 0.9
Carbohydrate %	< 45
Fat (ether extract) %	Maximum 0.12
Ash %	17-25
Humid	Maximum 0.8
pH	5-7
Density (g L^-1)	500
Heavy metals (ppm)	<10

Experimental groups	Hatchability %	Embryonic death %
TA 008	58.00^b	8.00^d
TA 049	54.00^d	11.00^c
TA 010	55.00^c	11.00^c
Polysorb	47.00^e	14.00^b
Positive control	46.00^f	18.00^a
Negative control	60.00^a	8.00^d
SEM	0.00	0.00
P value	<0.0001	<0.0001

Treatments	Body weight gain (g)		Feed intake (g)		Feed conversion ratio
	0-21 day	21-42 day	0-21 day	21-42 day	0-21 day	21-42 day
TA008	100.00	112.5	138.75^c	365.00	4.76	11.19
TA049	90.60	92.44	149.33^abc	375.25	4.97	15.80
TA010	87.90	110.00	166.56^ab	422.50	5.64	12.86
Polysorb	85.00	96.25	146.02^ab	382.50	5.15	12.52
PC	90.00	70.75	158.83^abc	372.50	5.01	14.94
NC	90.00	91.25	171.50^a	442.50	5.39	12.23
SEM	4.3	11.3	7.00	36.3	0.2	1.3
P value	0.292	0.173	0.030	0.598	0.149	0.175

Treatments	Proventriculus and Gizzard (g 100 g^-1 BW)	Fat (g 100 g^-1 BW	Duodenum (g 100g^-1 BW)	Jejunum (g 100g^-1 BW)	Ilium (g 100g^-1 BW)
TA 008	2.49	0.385	1.08	1.08	0.837
TA 049	2.43	0.684	1.27	1.38	0.888
TA 010	2.40	0.385	1.20	1.14	0.957
Polysorb	2.58	0.658	1.22	1.13	0.868
PC	2.85	0.743	1.22	1.17	0.811
NC	2.89	0.498	1.25	1.29	0.984
SEM	0.012	0.10	0.05	0.08	0.10
P value	0.015	0.066	0.371	0.224	0.860

Treatments	Bursa fabricius (g 100 g^-1 body weight)	Liver (g 100 g^-1 body weight)	Spleen (g 100 g^-1 body weight)
TA 008	0.12^a	2.93^c	0.045^b
TA 049	0.064^b	3.24^bc	0.041^b
TA 010	0.092^ab	3.70^ab	0.061^ab
Polysorb	0.086^ab	3.46^abc	0.048^b
PC	0.065^b	4.06^a	0.082^a
NC	0.092^ab	3.41^abc	0.057^ab
SEM	0.010	0.200	0.007
P value	0.034	0.0123	0.0192

Treatments	Albumin (g dL^-1)	Cholesterol (mg dL^-1)	Glucose (mg dL^-1)	HDL (mg dL^-1)	Triglyceride (mg dL^-1)	Total protein (g dL^-1)	Acid uric (mg dL^-1)	Globulin (g dL^-1)	LDL (mg dL^-1)
TA 008	2.05^a	186.75^b	214.14^a	45.25^a	186.89	4.62^a	5.38^c	2.37^ab	101.33
TA 049	2.08^a	196.42^ab	201.40^ab	43.46^ab	234.93	4.69^a	6.11^ab	2.60^a	105.96
TA 010	2.04^a	187.98^b	202.23^ab	40.01^c	230.21	4.30^ab	5.61^c	2.25^bc	109.09
Polysorb	2.16^a	186.75^b	192.17^bc	41.75^bc	227.29	4.15^b	6.40^a	1.98^c	99.53
PC	1.81^b	219.07^a	193.72^abc	40.63^bc	204.14	4.17^b	6.52^a	2.36^ab	126.53
NC	2.11^a	219.07^a	177.87^c	39.37^c	235.55	4.44^ab	5.83^bc	2.33^ab	104.36
SEM	0.07	7.60	6.74	1.10	15.80	1.00	0.14	1.00	6.66
P value	0.004	0.0045	0.015	0.002	0.191	0.020	<0.0001	0.012	0.083