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Somatic embryogenesis from immature embryos in maize genotypes

Eloise Helena Fernandes Alberto José Prioli Carlos Alberto Scapim Ivan Schuster Elisa Serra Negra Vieira Antônio Teixeira do Amaral Júnior Lia Mara Moterle About the authors

The establishment of a protocol for regenerating plants by tissue culture is the first step in breeding programs which have the objective of using genetic transformation on plants. The plant regeneration can be achieved either by organogenesis or embryogenesis. In the second case, the somatic embryogenesis depends on the identification of responsive genotypes which enhance the efficiency of the program. The aim of the present study was to identify maize genotypes with high capacity to produce somatic embryos and consequently regenerate maize plants. Eleven genotypes (inbreds and hybrids) were investigated in the present experiment. Under two-week periods, the cultures were obtained from immature embryos which were inoculated into growing medium N6 with 690mg L-1 of proline and 10mM of 2,4-D. Callis of type II, friable and embryogenic, were observed in the LD82025, CD308, and CML314 genotypes. After, they were submitted to the regeneration process and the best performance was achieved by the LD82025. No embryogenic callus was developed from CD307, CD304, OC-705, 105-B, and GU04328. In the present case, the inbred LD82025 is the most promising maize genotype for participating in a breeding program that will use the genetic transformation of maize plants.

Zea mays; tissue culture; regeneration


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