The platelets arrive quickly at the injury site and release several growth factors (GF) and citokines that contribute to bone repair and increasing local vascularization. The Platelet-rich Plasma (PRP) concentrates the platelets and their growth factors, increasing the rate of bone formation and better quality of trabecular bone. This research presents a protocol to PRP formulation and demonstrates some aspects about the use in canine bone repair. In this protocol blood was obtained from the jugular ven of tree adult dogs with medium weight of 20kg to produce PRP. Two defects in the medial aspect of proximal third of the tibia were surgically created to evaluate the clinical and radiographic aspect of PRP. The control defect wasn’t treated.The defect 2 was filled with 3 mg of autogenous bone graft from the tibia crest. The defect 3 was filled with PRP alone and the number 4 with PRP in the combination with 3mg autogenous bone graft. The proposed laboratory protocol demonstrated to be easy to execute at low cost. Further, it was adequate to concentrate platelets in final PRP, whose number was dependent on the blood from each dog. Comparing the defect regions, was concluded that the association of PRP and bone graft showed greater precocity and uniform radiopacity than the PRP or bone graft isolated, although both determine better results than the defect without treatment.
platelet gel; autogenous graft; growth factors
