Bipolaris euphorbiae Muchovej & Carvalho is considered a potential bioagent for control of selvage peanuts (Euphorbia heterophylla L.) on soybean crops in Brazil. However, little is known about the nutritional characteristics of this fungus. The main objective of this research was to evaluate different sources of carbon (glucose, starch, sucrose, lactose and maltose), of nitrogen (sodium nitrate, ammonium nitrate, ammonium sulphate, bi-basic ammonium phosphate, ammonium chloride), of phosphorus (monobasic potassium phosphate, bi-basic potassium phosphate, calcium phosphate, sodium phosphate, phosphoric acid, ammonium phosphate) and addition of organic sources of macro and micronutrients as supplement in the culture medium for the growth and sporulation of B. euphorbiae. Starch was the carbon source that promoted the greater fungus growth and was considered the most favorable to sporulation. The nitrogen source sodium nitrate and the phosphorus source monobasic potassium phosphate and calcium phosphate provided the best growth and sporulation of B. euphorbiae, but the others phosphorus source analyzed, except phosphoric acid, favored the fungus sporulation. The supplementation of the culture medium with peptone and yeast extract promoted a better growth and sporulation of the fungus and the addition of vitamins favored the production of conidia.
weed; biological control; culture medium; supplements