Abstracts
Two novel indole alkaloids with plumeran skeleton, spruceanumines A (1) and B (2), and eight known indole alkaloids, aspidospermidine (3), demethoxypalosine (4), aspidocarpine (5), aspidolimine (6), fendlerine (7), aspidolimidine (8), obscurinervidine (9) and obscurinervine (10) were isolated from stem bark and seeds methanolic extracts of Aspidosperma spruceanum. Compounds structures were elucidated on the basis of spectroscopic data, mainly those obtained by ¹H and 13C NMR (1D and 2D) and mass spectrometry.
Aspidosperma spruceanum; Apocynaceae; plumeran indole alkaloids
Dois novos alcalóides indólicos com esqueleto plumerano, spruceanuminas A (1) e B (2), e oito alcalóides indólicos conhecidos, aspidospermidina (3), desmetoxipalosina (4), aspidocarpina (5), aspidolimina (6), fendlerina (7), aspidolimidina (8), obscurinervidina (9) e obscurinervina (10), foram isolados do extrato metanólico das cascas do caule e sementes de Aspidosperma spruceanum. As estruturas dos compostos foram elucidadas com base na análise de dados espectroscópicos, principalmente os obtidos por espectros de RMN ¹H e 13C (1D e 2D) e por espectrometria de massas.
ARTICLE
Spruceanumines A and B, novel plumeran indole alkaloids from Aspidosperma spruceanum (Apocynaceae)
Vilma B. OliveiraI; Ivo J. Curcino VieiraI; R. Braz-FilhoII, *; Leda MathiasI; Norberto P. LopesIII; Antonio E. M. CrottiIV; Daniel E. de A. UchôaV
ILaboratório de Ciências Químicas (LCQUI)-CCT, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), 28013-602 Campos dos Goytacazes-RJ, Brazil
IIPesquisador Visitante Emérito-FAPERJ, Laboratório de Ciências Químicas(LCQUI)-CCT-UENF/PPGQO-DEQUIMUFRRJ, 28013-602 Campos dos Goytacazes-RJ, Brazil
IIIFaculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, 14040-903 Ribeirão Preto-SP, Brazil
IVNúcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, 14404-600 Franca-SP, Brazil
VCentro Nordestino de Aplicação e Uso da Ressonância Magnética Nuclear, Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, 60021-97 Fortaleza-CE, Brazil
ABSTRACT
Two novel indole alkaloids with plumeran skeleton, spruceanumines A (1) and B (2), and eight known indole alkaloids, aspidospermidine (3), demethoxypalosine (4), aspidocarpine (5), aspidolimine (6), fendlerine (7), aspidolimidine (8), obscurinervidine (9) and obscurinervine (10) were isolated from stem bark and seeds methanolic extracts of Aspidosperma spruceanum. Compounds structures were elucidated on the basis of spectroscopic data, mainly those obtained by 1H and 13C NMR (1D and 2D) and mass spectrometry.
Keywords:Aspidosperma spruceanum, Apocynaceae, plumeran indole alkaloids
RESUMO
Dois novos alcalóides indólicos com esqueleto plumerano, spruceanuminas A (1) e B (2), e oito alcalóides indólicos conhecidos, aspidospermidina (3), desmetoxipalosina (4), aspidocarpina (5), aspidolimina (6), fendlerina (7), aspidolimidina (8), obscurinervidina (9) e obscurinervina (10), foram isolados do extrato metanólico das cascas do caule e sementes de Aspidosperma spruceanum. As estruturas dos compostos foram elucidadas com base na análise de dados espectroscópicos, principalmente os obtidos por espectros de RMN 1H e 13C (1D e 2D) e por espectrometria de massas.
Introduction
The Aspidosperma(Apocynaceae) genus is endemic to Americas and is found mainly in regions between Mexico and Argentina.1 Aspidospermagenus continues to be fascinating as an expressive source of indole alkaloids with novel skeletons, which are interesting from a biosynthetic perspective and reported biological properties. Several species of Aspidospermaare broadly used in popular medicine as potential antimalarial agents, leishmaniose treatment, uterus and ovary inflammation, as contraceptive, in diabetes, in stomach problems, against cancer, fever and rheumatism.2
Aspidosperma spruceanum (A. spruceanum ), commonly known as "Paratudo-Branco" inAtlantic forest in the North of Espírito Santo State, appears as a tree of 5-20 m. The isolation and structure elucidation of two alkaloids from stem bark of A. spruceanumcollected in Rio de Janeiro State, Brazil, were reported.3
In the present paper, we describe the isolation and characterization of two novel plumeran indole alkaloids named as spruceanumines A (1) and B (2), along with known indole alkaloids: aspidospermidine (3),4-7 demethoxypalosine (4),7-9 aspidocarpine (5),8,10,14 aspidolimine (6),8,14 fendlerine (7),15,16 aspidolimidine (8),8,13,15 obscurinervidine (9)14,17 and obscurinervine (10).14,17 Their structures were established by spectrometric techniques, mainly one-and two-dimensional nuclear magnetic resonance (NMR), as well as high resolution electron spray ionization mass spectra (HRESIMS).
Results and Discussion
Elaboration of stem bark and seeds methanol extract of A. spruceanumby classical chromatographic methods resulted in the isolation of ten plumeran indole alkaloids (1-10), whose structures are shown in Figure 1. The well-known plumeran indole alkaloids, aspidospermidine (3), demethoxypalosine (4), aspidocarpine (5), aspidolimine (6), fendlerine (7), aspidolimidine (8), obscurinervidine (9) and obscurinervine (10) were identified on the basis of 1H and 13C NMR spectral data, including 1H-1H correlation spectroscopy (COSY), 1H-1H nuclear overhauser effect spectroscopy (NOESY), heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) NMR experiments,18 which were also used to complete unambiguous 1H and 13C chemical shift assignments of 1 and 2.
Spruceanumines A (1) and B (2), were obtained as a mixture of amorphous form, = -101.7 (CHCl3, c0.61). Infrared (IR) spectrum showed bands at Vmax 3100-2890 (C-H stretching), Vmax 1755 (stretching of the γ-lactone carbonyl group) in addition to other bands at Vmax 1624, 1606 and 1497 (C=C stretching of the benzene ring), and 887 and 739 cm-1 (C-H bending of substituted benzene ring).19
Comparative analysis of the {1H}- and distortionless enhancement by polarization transfer (DEPT) 135º- 13C NMR spectra (Table 1) revealed signals corresponding to 24 (1) or 25 (2) carbon atoms, allowing to recognize the presence of signals corresponding to nine nonhydrogenated [(C)9: three sp3 (including one bounded to nitrogen and oxygen atoms at δC 106.79), six sp2 (including one carbonyl group at δC 175.10 and five sp2 attributed to aromatic ring], five methine [(CH)5: two sp3 linked to nitrogen atom (δC 68.91/δH 3.50 and δc44.73/δH 3.27 correlated in the HSQC pectrum with 1H chemical shifts at δH 3.50 and 3.27, respectively, as indicated also in the direct subsequent correlations, 1JCH) and three sp2 (one aromatic at δC 101.78/ δH 6.63 (s) and two olefinic at δc123.31/δH 5.81 (ddd) and 130.79/δH 5.37 (brd)], seven (1) and eight (2) sp3 methylene [(CH)or (CH), including one linked to oxygen atom at δC 72.26 (1) and 70.20 (2, revealing γ-effect of the methyl group CH-4')] and three methyl [(CH): δc15.10/δH 1.12 (d, J= 6.2 Hz), 1; δC 9.39/δH 0.98 (t, J= 7.5 Hz), 2; and (MeO)2 represented by signals at δc 56.49/δH 3.70 (s) and 61.18/δH 3.81 (s), 1; δc 56.97/δH 3.74 (s) and 61.18/δH 3.86 (s), 2] carbon atoms, allowing to deduce the expanded molecular formulae (C)7(C=O)(N-C-O)(CH)5(O-CH2)(CH2)6 (CH3)(MeO)2and (C)7(C=O)(N-C-O)(CH)5(O-CH2) (CH2)7(CH3)(MeO)2 for 1and 2, respectively. This later contains additional metylene group CH2 (δC 22.56/δH 1.69 (m) and 1.46 (m) coupled to the hydrogens of an adjacent methyl group (δC 9.39/δH 0.98 (t, J= 7.5 Hz).
The high resolution electro-spray ionization mass spectrum (ESI-MS) of 1and 2showed peaks corresponding to the protonated molecules [M+H]+ at m/z425.2170 of 1(C24H29N2O5= m/z425.2076, Δm/z 0.0094) and 439.2332 of 2 (C25H31N2O5 = m/z439.2233, Δm/z 0.0099) Daltons, which together with the NMR 13C spectrum enable to propose molecular formulas C24H28N2O5 (1) and C24H28N2O5 (2), respectively, containing twelve degrees of unsaturation (C24H28N2O5 - C24H28 N2O5 = H24 or C25H54N2O5 -C24H30N2O5 = H24), which is consistent with the structure of alkaloids containing the nucleus of 21-oxo-aspidoalbidine20 (11, aspidospermidin-18,21-olide, using actual numeration) as basic structure (eleven degrees of unsaturation = four corresponding to aromatic ring, two to carbonyl lactone group and additional pentacyclic moiety), which after the location of one 1,2-disubstituted double bond between the carbon atoms CH-14 and CH-15 and of one heterocyclic involving the N-substituent and the oxygen atom sustained by carbon atom C-12, justifying the presence of OCH2 (1: δc 72.26/δH 4.27 and 3.90; 2: δH 70.20/δH 4.35 and 4.00, revealing shielding induced by γ-effect of the methyl 3H-4'), methyl group represented by a doublet signal (J= 6.2 Hz) at δH 1.12 (3H-3' correlated in the HSQC spectrum with 13C chemical shift at δC 15.10) coupled hydrogen linked to nitrogenated carbon atom (δH 3.27 , m, H-2' correlated with 13C signal at δC 44.73, CH-2') in the alkaloid 1and by a triplet signal (J= 7.5 Hz) at 0.98 (3H-4') coupled to hydrogen atoms of the additional methylene of 2 (δH 1.69 and 1.46 correlated in the HSQC with 13C chemical shift at δC 22.56). The lower field 13C chemical shift CH-2' (δC 50.43) in compound 2when to that of 1 (δC 44.73) is indicative of a β-effect induced by the methyl group CH3-4', as shown in Table 1.
The identity of the six-membered heterocyclic ring containing and oxygen, was supported by 3JCH HMBC correlations between C-12 [(δC 136.24, 1and 2)] and 2H-1' [δH 4.27 and 3.90 (1); δH 4.35 and 4.00 (2) (Table 1), as well as by 1H-1H-COSY cross-peaks displayed by H-1'b (δH 4.27 in 1; 4.35 in 2), H-1'a (δH 3.90 in 1; 4.00 in 2), H-2' (δH 3.27 in 1; 3.13 in 2).
The 1H-1H-COSY spectrum (Table 1) showed coupling of methylenic hydrogens at δH 4.27 [(dd, J= 10.7 and 2.7 Hz, H-1'b (1)] and δH 3.90 [(dd, J= 10.7 and 8.8 Hz, H-1'a (1)] with the methinic hydrogen at δH 3.27 (m, H-2', 1) and at δH 4.35 [(dd, J= 10.8 and 2.6 Hz, H-1'b (2)] and δH 4.00 [(dd, J= 10.8 and 8.6 Hz, H-1'a (2)] correlated with the signal at δH 3.13 [(m, H-2', 2)], in agreement with the presence six-membered ring formation.
The assignment of a methyl group at C-2' was confirmed by its 1H-1H-COSY and 3JCH HMBC correlations with H-2' (δH 3.27) and 2H-1' (δH 4.35 and 4.00), respectively.
In spruceanumine B (2), the presence of an ethyl group at C-2 was confirmed by the coupling of the methylenic hydrogens CH2-3' (δH 1.69 and 1.46) with the vicinal methyl group (δH 0.98) and H-2' (δH 3.13).
The 1H NMR spectrum of mixture showed signals at δH 3.70 (1), 3.74 (2) and δH 3.81 (1), 3.86 (2), which are characteristics of methoxyl groups linked to the benzene ring.19 These signals showed heteronuclear interaction via one bond (1JCH) with the signals at δC 56.49 (1), 56.97 (2) and 61.18 (1e 2) observed in the HSQC spectrum, suggesting the presence of two methoxyl groups linked to the ring A. This, was confirmed by long range heteronuclear coupling (nJCH, n= 2 and 3) observed in the HMBC spectrum, as summarized in Table 1. The signal at δC 61.18 (Table 1) observed in the 13C NMR of 1and 2is a typical value corresponding to signal of methoxyl groups located at forbidden position (MeO-11), as also observed in the aromatic ring of 11(MeO-11). These data allowed to and postulate the same substitution for 1and 2, as indicated in Figure 1.
The 13C NMR spectrum (Table 1) revealed the presence of a γ-lactone covering the carbon atoms C-20 e CH-21 by the signal at δC 175.10 (C-18), consistent with carbonyl carbon lactone of five members,20-21 that was also confirmed by long-range coupling of C-18 (δC 175.10) with both hydrogen atoms 2H-19 represented by the signals at δH 2.50 (H-19b) and δH 2.12 (H-19a). Additional heteronuclear long-range couplings are summarized in Table 1.
The main ions fragments observed in the ESI-MS/MS spectrum (low resolution) of 1and 2are summarized in Scheme 1. These fragmentation pattern are compatible with that of plumeran alkaloids, as 21-oxo-aspidoalbidine (18-oxo by actual numeration utilized in the literature), previously isolated from Aspidosperma exalatum20, and they are also in agreement with the presence of 18, 21-olide function in 1and 2, as suggested by signals at δC 175.10 (C-18) and 106.79 (C-21).
The location of a double bond at CH-14, CH-15 was deduced from the HMBC correlations of carbons resonating at δC 123.51 (CH-14, 1and 2), 130.79 (CH-15, 1) and 130.66 (CH-15, 2), with olefinic hydrogens at δH 5.81 (H-14), and δH 5.37 (H-15). The vicinal coupling between these hydrogen atoms was confirmed in the 1H-1H-COSY spectrum.18,21
The relative stereochemistry of spruceanumine A (1) and B (2) was suggested from the nuclear overhauser effect (nOe) interactions displayed in the NOE spectrum, as summarized in Figure 2.
1H-1H-NOESY correlations of H-2 and H-2' of 1 and 2 indicated both a-orientations; of H-2 with one hydrogen H-6 of the methylene group CH2-6 of 1 and 2 was also used to establish the relative configuration 7(S); of H-2 with both H-2' and 2H-3 of the methylene group CH2-3' of 2 revealed a-orientation of H-2; of H-16β with methyl group CH3-3' of 1 and with methylene group CH2-3' of 2 are consistent with β orientation of this hydrogen atom H-16; spatial interaction of the of the H-15 with both H-19 and H-17 indicated to these hydrogen atoms a and β-orientation, respectively, as shown in Figure 2.
The relative intensity of 1H NMR signals from the methyl groups CH3-3' (1, δH 1.12) and CH3-4' (2, δH 0.98) was used to deduce the approximated percentage of the 32.9% and 67.1% to spruceanumine A (1) and, spruceanumine B (2) in the mixture, respectively.
Experimental
General Procedures
Measures of optic rotation were obtained on a Perkin Elmer 343 digital polarimeter. Melting points were obtained on a Microquímica MQRPF and were uncorrected. Fourier transform infrared spectroscopy (FTIR) spectra were recorded on a FTIR-8300 Shimadzu spectrometer using KBr disk. ESI-MS (high resolution) and ESI-MS/ MS (low resolution) mass spectra were obtained on a MICROMASSUltrOTOF-Q (Brüker Daltonics, Billerica, MA) mass spectrometer, using the positive ion mode of analysis. Chromatographic purifications were carried out over silica gel (70-230 mesh). Silica gel 60F254 was used in thin layer chromatography analysis.
1H and 13C NMR spectra were measured on a Brüker DRX500 spectrometer, equipped with inverse probes and field gradient, operating at 500 (1H) and 125 (13C) MHz. CDCl3 was used as solvent and tetramethylsilane (TMS) as internal reference. Chemical shifts are given in the δ scale (ppm) and coupling constants Jin Hz. One dimensional (1D) 1H and 13C NMR spectra were acquired under standard conditions by using a direct detection 5 mm 1H/13C dual probe. Standard pulse sequences were used for two dimensional spectra by using a multinuclear inverse detection 5 mm probe with field gradient.
Plant materials
The stem bark and seeds of A. spruceanumBenth ex. Mull. Arg. were collected in November 2004 at Reserva Florestal de Linhares, Linhares, Espírito Santo State, Brazil. A voucher specimen (CVRD-273) is deposited at the Reserva Florestal herbarium, Cia. Vale do Rio Doce, Linhares, Espírito Santo State.
Extraction and isolation
Dried and powdered stem bark (3.09 kg) and seeds (530.1 g) from A. spruceanumBenth ex. Mull Arg were extracted with methanol at room temperature, furnishing, after solvent evaporation, 63.7 g and 18.5 g of crude methanol extracts, respectively.
The methanol extract (63.7 g) from stem bark was successively partitioned with CH2Cl2/H2O. The CH2Cl2 fraction (7.7 g) was chromatographed over silica gel column with a gradient of hexane/ethyl acetate to afford ten fractions. Fraction 8 (475.8 mg) was rechromatographed over a silica gel column with a gradient of MeOH in CH2Cl2 yielding aspidolimine (6, 15.9 mg) and demethoxypalosine (4, 34.7 mg). Fraction 10 (364.5 mg) was rechromatographed over a silica gel column with a gradient of MeOH in CH2Cl2 supplying aspidocarpine (5, 97.9 mg) and aspidospermidine (3, 19.1 mg) alkaloids.
The methanol extract (18.5 g) from seeds was partitioned with CH2Cl2/H2O. CH2Cl2 fraction (7.4 g) was chromatographed over silica gel column with a gradient of CH2Cl2/methanol supplying six fractions. Fraction 3 (3.9 g) was rechromatographed over a silica gel column with a gradient of MeOH in CH2Cl2 supplying four fractions. Fraction 3.1 (74.6 mg) provided the spruceanumines A-B (1-2) alkaloids mixture. Fraction 3.2 (103.2 mg) was rechromatographed over a silica gel column with a gradient of MeOH in CH2Cl2 supplying five fractions. Fraction 3.2.2 (20.6 mg) yielded the fendlerine (7) and aspidolimidine (8) alkaloids mixture, and fraction 3.2.4 (68.2 mg) afforded a mixture of obscurinervidine (9) and obscurinervine (10).
Spruceanumine A(1)
Amorphous solid, mp 195ºC;
= -101.7º (CHCl3, c0.61); IR (KBr disk)V max/cm-1: 3100-2890 (C-H stretching), 1755 (C=O;) 1624, 1606, 1479 (benzene ring), 887, 739 (benzene ring). HRESI-MS ([M+H]+) Found: m/z425.2170. Calc. for C24H29N2O5+: 425.2071 (see Scheme 1); 1H and 13C NMR: see Table 1.Spruceanumine B (2)
Amorphous solid, mp 195ºC; = -101.7º(CHCl3, c0.61); IR (KBr disk) Vmax/cm-1: 3100-2890 (C-H stretching), 1755 (C=O;) 1624, 1606, 1479 (benzene ring), 887, 739 (benzene ring). HRESI-MS ([M+H]+) Found: m/z439.2233. Calc. for C25H31N2O5+: 439.2227 (see Scheme 1); 1H and 13C NMR: see Table 1.
Acknowledgements
The authors thank to Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ-RJ-Brazil) for a visitant research fellowship and grants, to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil) for a research fellowship and grants and to Centro Nordestino de Aplicação e Uso da Ressonância Magnética Nuclear (CENAUREMN), Universidade Federal do Ceará (UFC), Fortaleza, Ceará, for the facilities in the obtention of the NMR (1D and 2D) spectra.
Supplementary Information
Available free of charge at http://jbcs.org.br, as PDF file.
Received: November 17, 2008
Web Release Date: April 30, 2009
FAPESP helped in meeting the publication costs of this article.
Supplementary Information
IR, ESI-MS, ESI-MS/MS, 1H NMR, {1H)-13C NMR, DEPT 135º13C NMR, 1H-1H-COSY, 1H-1H-NOESY, HSQC (1JCH) and HMBC (nJCH, n=2 and 3) spectra of 1 and 2 are available free of charge at http://jbcs.sbq.org.br, as PDF file.
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Publication Dates
-
Publication in this collection
10 June 2009 -
Date of issue
2009
History
-
Received
17 Nov 2008 -
Accepted
30 Apr 2009