Considering th e morphology, diet and spatial distribution of Satanoperca pappaterra and Crenicichla britskii (Perciformes: Cichlidae) in the Upper Paraná River floodplain (Brazil), the following questions were investigated: (1) Could the body shape predict the use of trophic resources and habitat by C. britskii and S. pappaterra? (2) Could the relationship between morphology and use of trophic resources and habitat be also extended to the intraspecific scale? (3) What are the most important morphological traits used to predict the variation on diet and habitat occupation within and between species? We hypothesized that intra and interspecific differences in morphological patterns imply in different forms of resource exploitation and that the ecomorphological analysis enables the identification of trophic and spatial niche segregation. Fish samplings were performed in different types of habitats (rivers, secondary channels, connected and disconnected lagoons) in the Upper Paraná River floodplain. Analyses of the stomach content was conducted to characterize the feeding patterns and twenty-two ecomorphological indices were calculated from linear morphological measurements and areas. A principal component analysis (PCA) run with these indices evidenced the formation of two significant axes, revealing in the axis 1 an ecomorphological ordination according to the type of habitat, regardless the species. The individuals of both species exploiting lotic habitats tended to have morphological traits that enable rapid progressive and retrograde movements, braking and continuous swimming, whereas individuals found in lentic and semi-lotic habitats presented morphology adapted to a greater maneuverability and stabilization in deflections. On the other hand the axis 2 evidenced a segregation related to the feeding ecology, between S. pappaterra and C. britskii. The relationship between morphology and use of spatial and feeding resource was corroborated by the Mantel test performed at inter and intraspecific levels. Therefore the hypothesis was accepted suggesting that analyses incorporating both intraspecific and interspecific morphological variations can contribute to a greater understanding about the ecological structure of fish assemblages by providing evidences on the niche characteristics of each species.
Crenicichla britskii; Ecomorphology; Ecomorphotype; Satanoperca pappaterra; Upper Paraná River floodplain
RESUMOConsiderando a morfologia, a dieta e a distribuição espacial de Satanoperca pappaterra e Crenicichla britskii (Perciformes: Cichlidae) na planície de inudação do alto rio Paraná (Brasil) as seguintes questões foram investigadas: (1) A forma do corpo pode ser utilizada para predizer o uso dos recursos espaciais e tráficos por ambas as espécie? (2) As relações entre morfologia e uso dos recursos tráficos e espaciais podem ser estendidas à escala intraespecífica? (3) Quais são as características morfológicas utilizadas para predizer a variação na dieta e ocupação do em nível intra e interespecífico? Testou-se a hipótese de que diferenças intra e interespecíficas nos padrões morfológicos implicam em diferentes formas de exploração dos recursos, sendo que a partir de análises ecomorfológicas é possível identificar a segregação do nicho tráfico e espacial. Os peixes foram amostrados em diferentes tipos de hábitats (rios, canais secundários, lagoas conectadas e desconectadas) na planície de inudação do alto rio Paraná. Análises de conteúdo estomacal foram realizadas a fim de caracterizar os padrões alimentares, enquanto vinte e dois índices ecomorfológicos foram calculados com base nas medidas morfométricas lineares e áreas. A análise de componentes principais (PCA) realizada com os referidos índices evidenciou a formação de dois eixos significativos: no eixo 1 houve uma ordenação ecomorfológica de acordo com o tipo de hábitat explorado, independentemente da espécie considerada. Nesse sentido, indivíduos de ambas as espécie coletados em ambientes lóticos tenderam a apresentar características morfológicas que propiciam maior capacidade de movimentos progressivos e retrógrados, frenagens e natação contínua, enquanto os indivíduos encontrados em ambientes lênticos e semi-lóticos apresentaram morfologia adaptada à maior capacidade de manobrabilidade e estabilização em guinadas. Por outro lado, o eixo 2 evidenciou segregação ecomorfológica relacionada à dieta, revelando uma divergência entre S. pappaterra e C. britskii. Essa relação entre morfologia e uso dos recursos espacial e alimentar foi confirmada pela significância do teste de Mantel realizado em nível intra e interespecífico. Portanto, a hipótese pressuposta foi aceita, sugerindo que análises que incorporam variações morfológicas intra e interespecíficas podem contribuir para o maior entendimento sobre a estrutura das assembleias de peixes, propiciando evidências acerca das características do nicho de cada espécie.
Ecomorphology is a branch of environmental sciences that deals with relationships between morphology and variations in the resource use in individuals, populations, guilds and assemblages (Peres-Neto, 1999; Oliveira et al., 2010). Ecomorphological studies seek to understand how organisms respond to environmental pressures, and may reveal patterns of association between body shape and the resources use (Gatz Jr., 1979; Wikramanayake, 1990; Ricklefs & Miles, 1994; Motta et al., 1995; Wainwright & Richard, 1995; Delariva & Agostinho, 2001; Freire & Agostinho, 2001; Xie et al., 2001; Casatti & Castro, 2006; Oliveira et al., 2010).
Cichlids comprise a group of fish widely distributed throughout the Neotropical regions and have singular morphological characteristics that allow them to live under diverse environmental conditions (Lowe-McConnell, 1991; Meyer, 1993). Some authors have reported correlations between body shape, diet and habitat among Cichlid species, where differences in morphology were used to explain differences in the exploitation of trophic and spatial resources (Norton & Brainerd, 1993; Norton, 1995; Winemiller, et al. 1995; Montaña & Winemiller, 2009). These studies analyzed the distribution of species in the interspecific multivariate ecomorphological space and argued that some patterns may be related to the evolutionary convergence or divergence of the body shape. Likewise, intraspecific ecomorphological approaches in Cichlids have evidenced that differences in feeding apparatus within the same population facilitate resource partitioning and reduce the competition (Swanson et al., 2003).
Despite these close relationships previously reported, associations between body shape and resource use are not ubiquitous (Douglas & Matthews, 1992) and instances of ecomorphological mismatch have been documented at inter and intraspecific level (Binning & Chapman, 2010; Griffen & Mosblack, 2011). Therefore ecomorphological analyses performed with sympatric and closely related species or even at the intraspecific level are important to test the role of morphology to predict ecology and may allow identifying adaptive ecomorphological patterns set by the selection process (Douglas & Matthews, 1992; Mittelbach et al., 1992), as well as to determine the main functional morphological traits used to explore the trophic and spatial niche (Delariva & Agostinho, 2001; Pagotto et al., 2011).
Crenicichla britskii Kullander, 1982 and Satanoperca pappaterra (Heckel, 1840) are two sympatric cichlid species; the first is endemic to the Upper Paraná River basin and the latter is widely distributed across some hydrographic basin in South America (Agostinho et al., 2004; Castro et al., 2004; Graça & Pavanelli, 2007). The singular trophic apparatus and body shape of these species may be one of the main characteristics responsible for explaining their successful colonization in aquatic ecosystems (Kullander, 2003; Sampaio & Goulart, 2011). Considering the morphology, diet and spatial distribution of these species in the Upper Paraná River floodplain, the following questions were investigated: (1) Could the body shape predict the use of trophic resources and habitat by
C. britskii and S. pappaterra? (2) Could the relationship between morphology and use of trophic resources and habitat be also extended to the intraspecific scale? (3) What are the most important morphological traits used to predict the variation on diet and habitat occupation within and between species? We hypothesized that intra and interspecific differences in morphological patterns imply in different forms of resource exploitation and that the ecomorphological analysis enables the identification of trophic and spatial niche segregation.
Material and Methods
Study area. The Paraná River is the second largest river of South America and the largest of the La Plata River basin, with more than 4,000 km length, 2.8 million km2 of catchment area and 500 million m3 of annual discharge with peak flows of 65,000 m3 s-1 (Maack, 1981; Bonetto, 1986). The study area covered the floodplain located in the third lower stretch of the upper Paraná River.
This floodplain is situated in the upper part of the Environmental Protection Area of Islands and Wetlands of the Paraná River, comprising the last dam-free stretch of the Paraná River in Brazilian territory, characterized by a meandering system containing lotic environments (rivers), semi-lotic (secondary channels s) and lentic (connected and disconnected lagoons), whose characteristics are listed in Table 1. Samplings were conducted at 36 sampling sites along these different types of habitats, belonging to the sub-basins of the rivers Paraná, Baía and Ivinhema.
Description of the sampled environments
Data collection. Samplings were performed in June, September, December 2009, and March and June 2010 in the different habitats of the Upper Paraná River floodplain (Table 1). Fish were captured by gill nets of different mesh sizes (2.4, 3, 4, 5, 6, 7, 8, 10, 12, 14 and 16 cm) exposed for 24 hours, and inspected every eight hours. Samplings were carried out through the Long Term Ecological Research (PELD - site 6). The captured individuals were taken to the field laboratory of the Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá (UEM), located in Porto Rico city, Paraná State, Brazil, where they were numbered, measured and weighed. The species identification was undertaken according to Graça & Pavanelli (2007). Voucher specimens of S. pappaterra (NUP 12349) and C. britskii (NUP 12350, NUP 12351) were deposited in the Ichthyological Collection of Nupélia, http:// peixe.nupelia.uem.br/.
Diet analysis. Fish were gutted and stomachs were fixed in 4% formaldehyde, and later preserved in alcohol 70° GL. Seventy stomachs of S. pappaterra and 17 of C. britskii were used to characterize the trophic ecology of these species. Stomach content analyses were performed under a stereomicroscope and an optical microscope whenever needed.
The diet analysis was performed by the occurrence method (percentage of stomachs containing each item in relation to the total of occurrences) and volumetric method, by which it was estimated, in percentage, the volume of each food item in relation to the volume present in all stomachs (Hyslop, 1980). Thus, the volume was quantified using grid Petri dishes, on which the food items were compressed with glass slides until 1mm height. The number of quadrants occupied by each food item on the dish was multiplied by 0.001 to obtain the volumes in mm3 (Hellawell & Abel, 1971). Given the absence of food in the stomach of many individuals, it was also analyzed the content of the first third of the intestine, but, for practical purposes, these were treated together, and referred to as stomach contents.
The food resources were grouped into nine categories: detritus, higher plant, algae, crustacean, aquatic insect, terrestrial invertebrate, mollusk, other aquatic invertebrate, and fish. To characterize the trophic patterns of each species, the volumetric and occurrence data of the items were combined in the Alimentary Index (IAi) (Kawakami & Vazzoler, 1980), expressed by:
where: i = food item; O = frequency of occurrence (%) of item i in the diet; V = volume (%) of item i in the diet.
Ecomorphological analysis. Nineteen linear morphometric measurements and six areas were taken (Table 2; see Oliveira et al., 2010 for further detail) from 89 individuals, 66 S. pappaterra and 23 C. britskii. Linear measurements up to 150 mm were taken with a digital caliper accurate to 0.01 mm, and larger measurements, using a metal ruler accurate to 1.0 mm. The areas of eyes and fins were obtained by drawing the contour of the structures in plastic sheets that were then digitized for the calculation of the areas using the software AutoCAD® (Autodesk, 2004). The measurements were taken from the left side of the specimens. The collection of morphometric data was performed with newly caught fish to minimize the effect of preservative substances on the morphometric variability (Peterson & VanderKooy, 1996). To reduce the influence of ontogenetic variation on the ecomorphological data, only adult individuals were used. In order to reduce the dimensionality of the variables, linear morphometric measurements were transformed, using the logarithm function as a parameter for standardization. Ecomorphological indices were calculated from these linear morphometric measurements and areas (Table 2), which expressed the shapes of morphological structures and hence reveal their ecological roles (Gatz Jr., 1979; Winemiller, 1991).
Ecomorphological indices with respective formulas and biological interpretations. Formulas include the following measurements: standard length (SL), maximum body height (MBH), body midline height (BMH), maximum body width (MBW), caudal peduncle length (CPdL), caudal peduncle height (CPdH), caudal peduncle width (CPdW), head length (HdL), head height (HdH), head width (HdW), length of snout with the mouth closed (LSC), length of snout with the mouth open (LSO), eye height (EH), mouth height (MH), mouth width (MW), caudal fin height (CH), anal fin length (AL), pectoral fin length (PtL), pelvic fin length (PvL), eye area (EA), dorsal fin area (DA), caudal fin area (CA), anal fin area (AA), pectoral fin area (PtA), pelvic fin area (PvA).
Data analysis. A principal component analysis (PCA) was applied on the covariance matrix formed by 22 ecomorphological indices for both species, aiming to summarize the multivariate morphological space into few dimensions that could better explain the organization of the original data set (Peres-Neto, 1999). To select the significant axes in the PCA, the broken-stick criterion was used (Jackson, 1993), in which the axes with eigenvalues higher than those generated at random were retained for interpretation. The scores were characterized according to the species type, the habitat where each individual fish was collected and the food resource type preferably exploited by each individual. A Multi-Response Permutation Procedure (MRPP) was employed for checking possible ecomorphological differences in the multivariate space occupied by these groups of scores. A Monte Carlo procedure with 10,000 randomizations was used to test the null hypothesis that the occupation of the ecomorphological space is not significantly different.
Mantel tests correlating the matrix of morphological distance with the matrix of trophic and spatial distance were performed for each species separately and also for both species together, in order to investigate whether higher similarity (smaller morphological distance) is related to the use of similar feeding resources (smaller trophic distance) and occupation of the same type of habitat (same spatial distance), at intra and interspecific level respectively. These tests were carried out through the Monte Carlo randomization procedure with 10,000 permutations, with the purpose to test the null hypothesis of no association between trophic, spatial and morphological patterns. The Mantel test was performed with 71 individuals, 58 S. pappaterra and 13 C. britskii.
The morphologic distance was obtained from the Euclidean distances between the ecomorphological indices for each pair of individuals, according to Gotelli & Ellison (2004). The trophic distance was obtained from the Bray-Curtis distance between the volume of the food item for each pair of individuals, ranging from 0 (maximum similarity) to 1 (maximum dissimilarity), according to Valentin (1995).
The matrix of spatial distance was obtained by assigning the value 1 to the pair of individuals from the same habitat and 0 to the pair of individuals that had not satisfied this condition. The ordination analyses (PCA), the MRPP, the calculation of trophic distance and morphological and Mantel test were performed using the software PC-Ord® 5.0 (McCune & Mefford, 1999).
Diet. In Table 3 are listed the food items that comprised the diet of both species, with respective volumes (V) and occurrences (O), in percentage, and also the alimentary index for each species, calculated for trophic categories, expressed in percentage. This index revealed that S. pappaterra primarily consumed detritus (IAi = 76.13%), whereas C. britskii consumed mostly fish (scale, fin, and others fragments of fish) (IAi = 50.00%) and crustacean (IAi = 19.03%).
Diet composition and alimentary index of Satanoperca pappaterra and Crenicichla britskii in the Upper Paraná River floodplain (%V = percentage of volume; %O = percentage of occurrence and IAi = alimentary index; NI = non-identified).
Ecomorphological analysis. The PCA evidenced the formation of two significant axes, according to the broken-stick model, and explained 74.19% total data variability (Table 4). The axis 1 revealed an ecomorphological ordination according to the type of habitat and the MRPP indicated that the ecomorphological patterns in each habitat type was significantly different (A = 0.037; p<0.01). The individuals exploiting lotic habitats tended to have positive scores and showed higher values of the aspect ratio of the anal, pelvic, pectoral and caudal fins (ARA, ARPv, ARPt and ARC), whereas the individuals exploiting lentic and semi-lotic habitats tended to have negative scores and showed larger pectoral, dorsal and anal fins, as well as larger eyes (Fig. 1a). This relationship between morphology and habitat was corroborated by the Mantel test performed at inter (r = 0.42, p<0.01) and intraspecific level (C. britskii: r = 0.42, p<0.01; S. pappaterra: r = 0.39; p<0.01).
Eigenvectors of morphological variables for axes 1 and 2 of the principal components analysis. The eigenvectors with the highest positive and negative values (in bold) were selected to interpret the species ordination in the multivariate morphological space. In the lower portion of the table are listed the eigenvalues and the percentage of variability explained by each principal component.
Dispersion of the scores of the first two PCA axes, calculated with the variance matrix of 22 ecomorphological indices. a) Scores classified by the type of environment; b) Scores classified by food resources, where: Emp = empty, Cru = crustacean, Aqu = aquatic insect, Fis = fish, Mol = mollusk, Hig = higher plant, Det = detritus. Dashed line: Crenicichla britskii; dotted line: Satanoperca pappaterra. ARA = Aspect ratio of the anal fin; ARC = Aspect ratio of the caudal fin; ARPt = Aspect ratio of the pectoral fin; ARPv = Aspect ratio of the pelvic fin; PI = Protrusion index; RAA = Relative area of the anal fin; RAD = Relative area of the dorsal fin; RAE = Relative area of the eye; RAPt = Relative area of the pectoral fin ; RAPv = Relative area of the pelvic fin ; RHM = Relative height of the mouth; RHPd = Relative width of the caudal peduncle; RWPd = Relative width of the caudal peduncle.
The axis 2 evidenced a segregation related to the morphological structures used to capture food items, revealing according to MRPP an ecomorphological segregation between S. pappaterra and C. britskii (A = 0.08; p<0.01). The first species, with negative scores, had higher values for the aspect ratio of the pelvic fin (ARPv), relative width of the caudal peduncle (RWPd), and relative areas of the pectoral and pelvic fins (RAPt and RAPv). On the other hand, C. britskii, with positive scores, had higher values for the relative areas of the anal fin (RAA), relative height of the mouth and peduncle (RHM and RHPd) and protrusion index (PI) (Fig. 1b; Fig. 2). The relationship between morphology and diet was corroborated by Mantel test performed at inter- (r = 0.65; p<0.01) and intraspecific level (C. britskii: r = 0.72, p<0.01; S. pappaterra: r = 0.63; p<0.01).
Head of Satanoperca pappaterra (a) and Crenicichla britskii (b), showing differences in the mouth protrusion.
Ecomorphology assumes that morphological attributes reflect important ecological characteristics, such as the use of resources, revealing the strategies adopted by the organisms and their adaptation to environmental conditions (Peres-Neto, 1999). In the present study, the hypothesis that differences in morphological patterns imply in different forms of resource exploitation was accepted, corroborating Oliveira et al. (2010) regarding the trend of the fish assemblage from the Upper Paraná River floodplain in establishing patterns of trophic and spatial niche exploitation according to ecomorphological adaptation of species. In addition, our results support the idea that morphology is a good predictor of the species ecology at inter- and intraspecific level. Thus such as reported by Oliveira et al. (2010), we found a significant correlation between morphology and spatial distribution at the interspecific level as well as the occurrence of two different ecomorphotypes: fish that exploit lotic and lower water habitats. However in the present study this trend was also registered within the same species. Thus the significant correlation between morphology and spatial distribution registered by the Mantel tests performed at intraspecific level evidenced that specimens collected in lotic habitats were morphologically more similar to each other than specimens from lentic habitats and vice versa.
According to the ordination along the ecomorphological multivariate space, both C. britskii and S. pappaterra collected in lotic habitats tended to have higher values of the aspect ratio of the anal, pelvic, pectoral, and caudal fins. These characteristics imply rapid progressive and retrograde movements, braking and continuous swimming, mainly in habitats with high current flow (Gatz Jr., 1979; Casatti & Castro, 1998; Breda et al., 2005). On the other hand, individuals of both species found in lentic and semi-lotic habitats presented morphological traits (larger dorsal, anal and pectoral fins) that provide greater maneuverability and stabilization in deflections (Gatz Jr., 1979; Watson & Balon, 1984; Breda et al., 2005). Maneuverability is defined as the ability of organisms to perform quick small-angled maneuvers (Webb et al., 1996). Thus, species with these characteristics exhibit a superior performance in the exploitation of structurally complex habitats with low current velocity (Oliveira et al., 2010; Chuang et al., 2006), such as connected and disconnected lagoons, where the presence of floating macrophyte stands contribute to increasing the environmental complexity. Conversely the intense sedimentation with predominance of mud and organic matter in the water column in these lentic habitats tend to promote a reduction in the mean value of water transparency (Thomaz et al., 2004), which may select those individuals with higher values of the eye area.
The second trend in the ecomorphological multivariate space was related to the feeding ecology. In this case it was recorded a morphological divergence between the species. Crenicichla britskii presented higher values of the relative height of the mouth and protrusion index. Such characteristics are associated with its ability to capture larger and evasive preys throughout the water column (Hulsey & Gracia de León, 2005; Cochran-Biederman & Winemiller, 2010), since the terminal position of the mouth and its higher protrusion (see Fig. 2) favor a wide oral aperture that enables a diet based on larger feeding items, such as crustacean, mollusk, fish and aquatic insect (Gibran et al., 2001; Casatti, 2002; Sampaio & Goulart, 2011). Furthermore, this species also present a well-developed anal fin and a higher caudal peduncle, which are functionally related to the higher maneuverability, movement stabilization and start-ups at short distance displacements (Breda et al., 2005; Oliveira et al., 2010). These characteristics contribute to the impulsion of the fish during the early stage of the attack (Lauder & Liem, 1981) and play an important role to perform sudden and interrupted displacements for the prey capture.
On the other hand, Satanoperca pappaterra has morphological traits (e.g. sub-terminal mouth and well-developed lips) that favor the capture of detritus and benthic invertebrates on the bottom, as recorded by Sampaio & Goulart (2011). The present study revealed that this species can be considered a good swimmer, since it has wider caudal peduncle and well-developed pelvic and pectoral fins, which indicate higher abilities in stabilization, braking and accelerations (Gatz Jr., 1979; Watson & Balon, 1984; Breda et al., 2005). According to Oliveira et al. (2010), well-developed caudal peduncles and pectoral fins are of paramount importance for benthic fish to stabilize the body on the substrate, as well as to start-ups at short distances displacements, which probably contributed to the feeding in the benthic region.
Although less clear than the interspecific variation in the ecomorphological space, individual divergences may also have an important role on the trophic niche occupation by both species. The significance of the Mantel test performed at intraspecific level evidenced that even subtle divergences in body shape are important to the capture of different food items, as evidenced by the consumption of aquatic insects by some specimens of C. britskii with higher values for the aspect-ratio of caudal, anal, pectoral and pelvic fins. This morphological design may favor a greater ability to swim (Breda et al., 2005) and capture aquatic insects along the water column or associated with submerged vegetation (Gibran et al., 2001). Therefore, such as the spatial dimension of the niche where the intraspecific morphological differentiation is related to the exploitation of habitats with distinct environmental conditions, trophic niche characteristics of each species are conditioned to its individual morphological variations. These results emphasize the need for recognizing the importance of differences within-species in studies of community ecology, which is rarely considered, especially in trait-based approaches encompassing Metazoan, where interspecific mean trait differences between co-occurring species are used to reveal structural patterns and community assembly processes, e.g. niche filtering and biotic interactions (Violle et al., 2012). Such approaches involving morphological diversity without any concern about intraspecific variations may be underestimating the real functional diversity exhibited by each species. According to Siefert (2012) intraspecific variation provides a more complete view of community structure patterns and processes driving their arrangement. Thus, as reported in our study, intraspecific variation in a range of individual traits determines resource-specific efficiency and preferences (Bolnick et al., 2003), being an indicative of the niche breadth occupied by the species (Van Valen, 1965; Bolnick et al., 2007).
In the present study, the ecomorphological analysis evidenced that variations in resource use are related to the body shape. In this way, the ecomorphology proved to be an effective tool able to contribute to the knowledge about the ecology of the species and allowed the understanding of how organisms respond to environmental pressures, which demonstrates its high predictive nature. Moreover our study supports that analyses incorporating both intraspecific and interspecific morphological variations can contribute to a greater understanding about the ecological structure of fish assemblages by providing evidences on the niche characteristics of each species.
The authors are grateful to Norma Segatti Hahn, Milza Fedatto Abelha, Fabrício B. Teresa and to two anonymous referees for their valuable suggestions for the improvement of this manuscript; Fabio Teruo Mise and Gabriel Deprá for their help with statistical analysis and drawings of fish, respectively; PEA/Nupélia/UEM/PROEX for logistic support; A. L. A. Sampaio and J. P. A. Pagotto are grateful to Coordination of Improvement of Higher Education Personnel (CAPES/Ministry of Education, Brazil) for the scholarships.
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Published June 28, 2013
Publication in this collection
24 May 2013
Date of issue
27 Apr 2012
14 Feb 2013