Microsatellites markers are a useful tool in much plant genetic analysis. Currently, few microsatellites loci have been reported in Carica papaya L.. Therefore, the objective of this study was accomplishing a search for microsatellites in the public available - GenBank / NCBI (National Center of Biotechnology Information), aiming the future use in molecular and genetic studies applied to genetic breeding. The sequences were collected from GenBank / NCBI in FASTA-formatted files and analyzed for the presence of microsatellites with a minimum of 20, 7 and 5 repetitions of mono-, di- and trinucleotides, respectively, and above of 4 repetitions for tetra- and pentanucleotides. The sequences with over 90% of similarity, were considered redundant and, therefore, eliminated of the analyses. A total of 44,591 sequences had been analyzed, of which 3,180 were not redundant, showing 3,947 microsatellites. Out of those, 3,587 were classified as perfect, 8 imperfects, 65 interrupted, 239 perfect-compounds, 8 imperfect-compounds and 40 interrupted-compounds. The dinucleotides and trinucleotides repetitions represented 65.7 and 14.4% of the whole analyzed sequences, respectively. Only the AT/TA motif represented 44.1% of the microsatellites sequences. The most common motifs of tri-, tetra- and pentanucleotides were AAT, AATT and TTTAA, respectively. In the available sequences it can be observed that the papaya genome has an average one microsatellite for every 5,65 kb.
molecular marker; Carica papaya L.; SSR; GenBank
