Hays et al.2424. Hays NP, Kim H, Wells AM, Kajkenova O, Evans WJ. Effects of whey and fortified collagen hydrolysate protein supplements on nitrogen balance and body composition in older women. J Am Diet Assoc 2009[acesso em 15 maio 2014];109(6):1082-87.
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Humans: nine elderly women (between 65-85 years of age) |
Whey protein (0.85 g/kg/weight/day) 15 days CH (0,81 g/kg/weight/day) 15 days |
Whey protein group:Lower weight; higher excretion of nitrogen CH group: Maintaining of weight; Lower excretion of nitrogen |
Maintaining of weight and muscle mass |
Guillerminet et al.2626. Guillerminet F, Beaupied H, Fabien-Soulé V, Tomé D, Benhamou CL, Roux C, et al. A. Hydrolyzed collagen improves bone metabolism and biomechanical parameters in ovariectomized mice: an in vitro and in vivo study. Bone 2010;46(3):827-34.
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In vivoMice |
CH 10 or 25 g/kg/weight/day 4-12 weeks Bone mineral density |
Higher growth and differentiation of osteoblasts; higher growth and differentiation of osteoclasts |
Osteoprotective action |
Jackix et al.2727. Jackix EA, Cúneo F, Amaya-Farfan J, Assunção JV, Quintaes KD. A food supplement of hydrolyzed collagen improves compositionaland biodynamic characteristics of vertebrae in ovariectomizedrats. J Med Food 2010;13(6):1-6.
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Rats, six groups |
CH 50 mg; 100 mg, control (gelatin) Sample of femur and vertebral column. |
Supported weight four-times heavier; Higher percentage of bone protein; Higher bone mineralization |
Greater conservation of the composition and bone strength |
Kim et al.2828. Kim HK, Kim MG, Leem KH. Osteogenic activity of collagen peptide via ERK/MAPK pathway mediated boosting of collagen synthesis and its therapeutic efficacy in osteoporotic bone by back-scattered electron imaging and microarchitecture analysis. Molecules [Internet] 2013 [acesso em 15 maio 2014];18(12):15474-89.Disponível em: http://www.mdpi.com/1420-3049/18/12/15474 http://www.mdpi.com/1420-3049/18/12/1547...
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In vitroHuman cells |
Gene COL1A1 |
Increased osteoblast differentiation by gene expression |
Osteoprotective action |
In vivoRats |
CH 150; 500 mg/kg/weight/day 12 weeks Vertebrae, lumbars |
Increase of bone mineral density |
Clark et al.3434. Clark KL, Sebastianelli W, Flechsenhar KR, Aukermann DF, Meza F, Millard RL, et al. 24-Week study on the use of collagen hydrolysate as a dietary supplement in athletes with activity-related joint pain. Curr Med Res Opin 2008;24(5):1485-96.
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Humans: 147 athletes |
CH 10 g placebo (xanthan gum) Inflammation, mobility and joint pain |
Significant improvement in pain (knee arthralgia) |
Reduction of pain, protection of cartilage |
Sugihara et al.3737. Sugihara F, Inoue N, Kuwamori M, Taniguchi M. Quantification of hydroxyprolyl-glycine (Hyp-Gly) in human blood after ingestion of collagen hydrolysate. J Biosci Bioeng 2012;113(2):202-3.
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Humans: five individuals |
CH 8 g Blood samples 0,5; 1; 2; 4 hours Pro-Hyp; Hyp-Gly |
Increase of AA, di- and tripeptides in peripheral blood |
Proliferation and cell growth, as well as protection of cartilage |
Hartog et al.3838. Hartog A, Cozijnsen M, Vrij G, Garssen J. Collagen hydrolysate inhibits zymosan-induced inflammation. Exp Biol Med 2013;238(7):798-802.
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Rats |
CH 12,5; 25; 50 mg Three consecutive days Induced inflammation in the ear Blood sample |
Higher concentration of glycine in plasma; swelling reduction; proinflammatory cytokines |
Potential reduction in pain (hip and knee) |
Shigemura et al.3939. Shigemura Y, Kubomura D, Sato Y, Sato K. Dose dependent changes in the levels of free and peptide forms of hydroxyproline in human plasma after collagen hydrolysate ingestion. Food Chem [Internet] 2014 [acesso em 16 maio 2014];159:328-32. Disponível em: http://www.sciencedirect.com/science/article/pii/S0308814614002763 http://www.sciencedirect.com/science/art...
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Humans: Healthy volunteers n= 4 |
Different doses of CH/kg/weight (30,8; 153,8; 384,6 mg/kg/weight Blood sample before, 15, 30, 60, 120, 240, 360 minutes after ingestion |
Dose-dependent increase of 6.43; 20.17; 32.84 nmol/mL in the plasma concentration of Hyp, respectively. |
Increase of Hp in plasma and potential of increase in amino acid absorption |
Bruyère et al.4040. Bruyère O, Zegels B, Leonori I, Rabenda V, Janssen A, Bourges C, Reginster JY. Effect of collagen hydrolysate in articular pain: A 6-month randomized, double-blind, placebo controlled study. Complement Ther Med [Internet] 2012 [acesso em 16 maio 2014];20(3):124-30. Disponível em: http://www.complementarytherapiesinmedicine.com/article/S0965-2299(12)00002-7/fulltext http://www.complementarytherapiesinmedic...
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Humans: 200 patients older than 50 years of age |
CH 12 g Placebo (gel capsules) |
6 month treatment, improvement in symptoms according to EVA-D scale; tolerability |
Efficacy and safety of supplementation |