Antioxidant effect of the guava byproduct in the diet of broilers in the starter phase

ABSTRACT

This work aimed to investigate the antioxidant capacity of the guava agroindustrial waste as a functional additive in broiler feed to improve the performance and meat quality of boilers. The experiment was conducted in a completely randomized design, consisting of four treatments and six replicates with 12 birds. Treatments included different levels of guava byproduct in the feed: 0, 0.5, 1.0, and 1.5%. We evaluated the performance of broilers at 7 and 21 days old. At 21 days old, two birds from each experimental unit were euthanized for intestine histologic evaluation (duodenum, jejunum, and ileum) and breast and deboned thigh samples were collected for analyzes of pH, colorimetry (L*, a*, and b*), and thiobarbituric reactive substances (TBARS). The use of guava byproduct in the diet at 7 days old did not affect feed intake and feed conversion of the birds. However, the body weight and weight gain increased linearly with the inclusion of the byproduct. At 21 days old, the guava byproduct did not depress the performance of birds. There was no effect of treatments on villus height, crypt depth, and villus:crypt ratio of the duodenum and jejunum of the birds. The inclusion of guava byproduct resulted in lower crypt depth and linear increase in villus:crypt ratio of the ileum. There was no significant difference in pH and colorimetry of the breast and thigh. With increasing inclusion of byproduct, TBARS value was reduced to 0.72%, indicating greater lipid stability in thigh meat in this inclusion amount. Guava byproduct can be used as an alternative antioxidant additive in broiler feed because it does not depress the productive performance and improves thigh meat quality of boilers.

Key Words:
animal-performance indexes; antioxidant; intestinal histology; meat characteristics; Psidium guajava L

Introduction

Byproducts of fruits have contributed to the increased production of organic waste, causing many environmental problems. Thus, studies have been conducted to check the nutritional value of the waste and suggest alternatives for its use (Souza et al., 2011).

Knowing these plants and applying them in consolidated production systems, as in animal production, brings benefits to both parties, as it allows food production, conservation of regional native species, and the use of agroindustrial byproducts. In addition, there are the incessant search for substitutes for synthetic products used in animal feed, which appear increasingly restricted by the importing countries of animal products produced in Brazil.

In this context, guava, Psidium guajava L., presents the potential to be used in broiler feed as a natural antioxidant. It has regular amount of acids, sugars, and pectin and presents tannins, flavonoids, essential oils, sesquiterpenoids alcohols, and acids triterpenoids in its constitution, which can reduce or inhibit oxidation of lipids or other molecules, preventing the spread of oxidation chain reactions (Iha et al., 2008). Nascimento et al. (2010) reported that, in lipid environment, the hydroacetonic and hydromethanolic extracts of the guava agroindustrial waste exhibit significant antioxidant action, acting efficiently in the initial stage of oxidation by blocking the reaction.

The replacement of synthetic by natural antioxidants presents advantages because they are not harmful to health. In the use of natural antioxidants, there is also an advantage in the preservationist level, as far as the food industry produce waste, which can have a beneficial target, favoring man and the environment. For example, guava undergoes processing for the production of juices, jams, pulps, and extracts. This fruit have skins and seeds, natural sources of antioxidants, which are often discarded rather than used. One of the byproducts generated in the guava industries is called “decanter” and consists of solid residue from the separation of the juice from the pulp. This byproduct is rich in phenolic antioxidants and is discharged by the juice industry; therefore, it can be used in animal diets.

Thus, with the high proportion of agroindustrial organic residues and the presence of guava bioactive phytochemicals, this work aimed to investigate the antioxidant capacity of the guava agroindustrial waste as a functional additive in broiler diet to improve performance and meat quality of broilers.

Material and Methods

The experiment was conducted in June and July 2014. All procedures in this study were conducted according to the Protocol registration CEUA/UFG 056/14 and approved by the Ethics Committee on the Use of Animals.

Two-hundred eighty-eight female broilers of Cobb500 strain were used. They were raised from one to 21 days old and purchased from a commercial hatchery. The birds were housed in batteries composed of galvanized wire cages with dimensions of 0.50 × 0.40 × 0.40 m. Each cage was equipped with a drinking fountain and a feeder on the front.

The experimental design was completely randomized, consisting of four treatments and six replicates with 12 birds each. The birds were weighed and divided into plots with uniform weights between them. The treatments included different levels of guava byproduct in pre-starter and starter diets: 0; 0.5; 1.0 and 1.5%.

The guava byproduct was purchased from a trading company, obtained through the decanter process, which separated the juice from the pulp, generating a solid residue, which is equivalent to approximately 10% of the volume of guava pulp processed. The determination of total phenolic compounds was carried out, being 0.17% determined by spectrophotometry, and the chemical analysis of the byproduct (Table 1).

Table 1
Chemical composition of guava byproduct

Parameter	g/kg dry matter
Dry matter	941.4
Mineral matter	10.0
Crude protein	39.5
Ethereal extract	16.3
Neutral detergent fiber	761.8
Acid detergent fiber	453.2
Phenol	1.7

The experimental diets were isonutritive, based on corn and soybean meal, meeting the nutritional requirements of each growth stage, according to the recommendations of Rostagno et al. (2011) (Table 2). The feeding program comprised two stages: pre-starter diet (1 to 7 days old) and starter diet (8 to 21 days old). The guava byproduct was added to the basal diet replacing starches.

Table 2
Basal feed composition and nutritional values for the pre-starter (1 to 7 days old) and starter (8 to 21 days old) stages

Item		Pre-starter stage	Starter stage
Ingredient (g/kg as fed)
	Corn grain	581.1	603.4
	Soybean meal 45%	346.1	325.5
	Soy oil	10.5	14.8
	Dicalcium phosphate	18.2	16.9
	Limestone	8.8	8.4
	Salt	4.8	4.6
	DL-Methionine 99%	3.8	2.6
	L-Lysine HCL	4.0	2.2
	L-Threonine	1.7	0.6
	Vitamin supplement1	0.5	0.5
	Mineral supplement2	0.5	0.5
	Starch	20.0	20.0
	Total	1,000.0	1,000.0
Composition
	Metabolizable energy (kcal/kg)	2,950	3,000
	Crude protein (g/kg as fed)	209.80	199.00
	Calcium (g/kg as fed)	8.91	8.39
	Available phosphorus (g/kg as fed)	4.48	4.21
	Sodium (g/kg as fed)	2.11	2.03
	Potassium (g/kg as fed)	8.01	7.70
	Digestible lysine (g/kg as fed)	13.16	11.26
	Digestible cystine + methionine (g/kg as fed)	9.34	7.99
	Digestible threonine (g/kg as fed)	8.55	7.32

• ¹
  Vitamin supplement (composition per kg of product): vitamin A, 3,125,000 IU; vitamin D3, 550,000 IU; vitamin E, 3,750 mg; vitamin K3, 625 mg; vitamin B1, 250 mg; vitamin B2, 1,125 mg; vitamin B6, 250 mg; vitamin B12, 3,750 mg; niacin, 9.500 mg; calcium pantothenate, 3,750 mg; folic acid, 125 mg; DL-methionine, 350,000 mg; choline chloride, 150,000 mg; selenium, 50 mg; antioxidant, 2.500 mg; vehicle (sufficient quantity to), 1,000 g.
• ²
  Mineral supplement (composition per kg of product): manganese, 150,000 mg; zinc, 100,000 mg; iron 100,000 mg; copper, 16,000 mg; iodine, 1,500 mg.

The birds received feed and water freely throughout the experimental period; the feeders were supplied with food twice a day to avoid waste. The cleaning of the drinking fountains was performed daily, as well as the exchange of water. The temperature and humidity were constantly monitored by thermometer and hygrometer, respectively, and the environment was controlled with the help of curtains and lighting management, according to the needs of the birds.

Performance was evaluated at 7 and 21 days old. The birds and feed were weighed weekly and the weight of dead birds were recorded daily. The analyzed variables were average feed intake, average body weight, weight gain, and feed conversion.

At 21 days old, two birds from each experimental unit, with the average weight of the portion, were euthanized by cervical dislocation for intestine histologic evaluation. For the preparation of histological blades, segments of 2.0 cm of the duodenum, jejunum, and ileum were collected and fixed in 10% formaldehyde buffered for 24 h. After fixation, they were stored in 70% ethanol, processed according to the methodology of Luna (1968), and stained with hematoxylineosin method.

The images were obtained in fivefold increase, using an optical microscope Leica DM 4000B coupled to a microcomputer. The images were analyzed with ImageJ software, with which were performed villus height and crypt depth measurements.

The quality of meat samples of breast and deboned thigh were analyzed. They were collected and placed in plastic bags and kept in cool boxes with ice for transport to the analysis laboratory, 24 h after slaughter.

Afterwards, the pH readings were made in triplicate in the ventral portion of the muscles using the portable pH meter (Brand: Testo, model 205). Colorimetry determinations (L*, a*, and b*) were performed at three different points in the ventral portion of the muscles using a colorimeter (Brand KONICA MINOLTA, model Chroma meter CR-400).

Subsequently, the samples were vacuum-packed and kept chilled in freezer for the analysis of thiobarbituric reactive substances (TBARS). The samples were mixed homogeneously, so that 10 g of each could be used; the acid was added and the sample was placed in boiling water bath for 50 min and then read in the spectrophotometer, according to the methodology of Tarladgis et al. (1960).

After verification of the normal residue data, they were subjected to variance analysis with 5% of significance; regression was performed between the levels of inclusion of guava byproduct, through R Software program.

Results

Feed intake and feed conversion of broilers were not affected by the use of guava byproduct in the diet of 1 to 7-day olds. However, the body weight and weight gain increased linearly with the inclusion of guava byproduct (P<0.05) (Table 3).

Table 3
Average body weight (BW), weight gain (WG), feed intake (FI), and feed conversion (FC) of seven-day-old broilers fed diets containing guava byproduct

Treatment		BW (g)	WG (g)	FI (g)	FC (g/g)
0% of guava byproduct		133.61	95.46	107.61	1.135
0.5% of guava byproduct		151.58	113.33	126.95	1.120
1.0% of guava byproduct		146.80	108.57	117.72	1.084
1.5% of guava byproduct		147.11	108.86	121.50	1.115
CV (%)		6.33	8.57	9.14	7.140
P-value	Linear	0.0321	0.0332	0.085	0.529
	Quadratic	0.056	0.056	0.175	0.459

• CV - coefficient of variation.
• ¹
  y=138.14+7.78x(R2=0.80).
• ²
  y=99.97+7.72x(R2=0.80).

At 21 days old, the guava byproduct did not affect broiler performance (Table 4). It is possible that the absence of guava byproduct effects on performance was reflecting the ideal conditions for development.

Table 4
Average body weight (BW), weight gain (WG), feed intake (FI), and feed conversion (FC) of 21-day-old broilers fed diets containing guava byproduct

Treatment		BW (g)	WG (g)	FI (g)	FC (g/g)
0% of guava byproduct		609.12	570.93	956.33	1.678
0.5% of guava byproduct		622.02	583.79	954.66	1.635
1.0% of guava byproduct		612.90	574.65	946.19	1.648
1.5% of guava byproduct		609.50	571.22	919.94	1.611
CV (%)		4.46	4.75	4.14	4.350
P-value	Linear	0.923	0.918	0.124	0.158
	Quadratic	0.541	0.575	0.460	0.983

• CV - coefficient of variation.

There was no effect of the use of guava byproduct on villus height, crypt depth, and villus:crypt ratio in the duodenum and jejunum of the birds (Tables 5 and 6).

Table 5
Villus height, crypt depth, and villus:crypt ratio of the duodenum of 21 -day-old broilers fed guava byproduct

Treatment		Villus height (μm)	Crypt depth (μm)	Villus:crypt ratio
0% of guava byproduct		583.45	76.99	7.87
0.5% of guava byproduct		587.60	87.45	7.25
1.0% of guava byproduct		612.90	85.35	7.26
1.5% of guava byproduct		623.13	67.87	9.40
CV (%)		15.21	23.08	25.17
P-value	Linear	0.418	0.344	0.201
	Quadratic	0.916	0.112	0.174

• CV - coefficient of variation.

Table 6
Villus height, crypt depth, and villus:crypt ratio of the jejunum of 21-day-old broilers fed guava byproduct

Treatment		Villus height (μm)	Crypt depth (μm)	Villus:crypt ratio
0% of guava byproduct		402.31	67.96	6.00
0.5% of guava byproduct		454.57	80.06	5.85
1.0% of guava byproduct		465.20	61.17	7.62
1.5% of guava byproduct		434.34	65.20	7.01
CV (%)		12.29	20.75	20.57
P-value	Linear	0.384	0.416	0.125
	Quadratic	0.125	0.387	0.916

• CV - coefficient of variation.

The ileum villus height was not influenced by the addition of the guava byproduct in the diet, but there was a significant result for the crypt depth and villus:crypt ratio variables (Table 7).

Table 7
Villus height, crypt depth, and villus:crypt ratio of the ileum of 21 -day-old broilers fed guava byproduct

Treatment		Villus height (μm)	Crypt depth (μm)	Villus:crypt ratio
0% of guava byproduct		369.13	82.11	4.51
0.5% of guava byproduct		332.79	64.48	5.31
1.0% of guava byproduct		373.06	65.12	6.27
1.5% of guava byproduct		474.84	61.94	7.11
CV (%)		27.30	15.97	25.25
P-value	Linear	0.504	0.0071	0.0192
	Quadratic	0.477	0.141	0.203

• CV - coefficient of variation.
• ¹
  y=77.896 −12.20x(R2=0.70).
• ²
  y=4.732 +1.381x(R2=0.63).

There was no significant difference in pH of the breast and thigh of broilers in the starter stage, fed different levels of the guava byproduct in the diet (Tables 8 and 9).

Table 8
Colorimetry and pH of the breast of 21 -day-old broilers fed guava byproduct

Treatment		L*	a*	b*	pH
0% of guava byproduct		40.65	4.38	6.36	5.96
0.5% of guava byproduct		39.65	4.05	5.36	5.90
1.0% of guava byproduct		40.53	4.81	6.68	5.95
1.5% of guava byproduct		40.89	4.32	6.49	5.96
CV (%)		3.96	25.99	15.91	2.59
P-value	Linear	0.653	0.824	0.448	0.739
	Quadratic	0.479	0.759	0.512	0.534

• ^L*
  lightness;
• ^a*
  red content;
• ^b*
  yellow content.
• CV - coefficient of variation.

Table 9
Colorimetry and pH of the thigh of 21-day-old broilers fed guava byproduct

Treatment		L*	a*	b*	pH
0% of guava byproduct		40.67	9.37	6.65	6.09
0.5% of guava byproduct		42.22	9.03	6.30	6.04
1.0% of guava byproduct		41.56	9.31	7.59	6.04
1.5% of guava byproduct		41.48	9.45	7.01	6.02
CV (%)		3.65	10.87	11.91	1.47
P-value	Linear	0.461	0.816	0.169	0.650
	Quadratic	0.276	0.628	0.531	0.351

• ^L*
  lightness;
• ^a*
  red content;
• ^b*
  yellow content.
• CV - coefficient of variation.

No significant effect of guava byproduct was observed for lightness (L*), red content (a*), and yellow content (b*) for breast meat (Table 8).

The L*, a*, and b* values for thigh meat also did not differ (Table 9).

In addition, there was no influence of the levels of guava byproduct on the average values of TBARS in the breast meat samples (Table 10).

Table 10
Thiobarbituric reactive substances (TBARS) resulting from lipid oxidation of thigh and breast meat samples of 21-day-old broilers fed guava byproduct

Treatment		TBARS mg/1000 g
		Thigh	Breast
0% of guava byproduct		0.1775	0.040
0.5% of guava byproduct		0.1324	0.061
1.0% of guava byproduct		0.1333	0.029
1.5% of guava byproduct		0.1108	0.046
CV (%)		25.10	27.15
P-value	Linear	0.726	0.856
	Quadratic	0.0311	0.893

• CV - coefficient of variation.
• ¹
  y=0.1254 +0.094x−0.0657x2(R2=0.39).

Discussion

The nutrient digestion and absorption capacity suffers significant adjustments in the post-hatching period and may be influenced by the feeding level and diet composition (Uni, et al, 1995; Longo et al., 2005). In this study, we could verify that, although feed intake was not affected by the addition of the byproduct in the diet, weight gain was improved.

Lira et al. (2009) evaluated the effect of including guava meal (0, 3, 6, 9, and 12%) as alternative feed for broilers and found that inclusion up to the level of 12% in the diet was similar to performance and carcass yield of broilers fed without residue, as in the result of this work when using the residue as a functional additive.

The villus height and crypt depth reflect the absorption of nutrient capacity: the larger the size and amount of the villus, the greater the area of absorption of nutrients (Furlan et al. 2004). However, in this study, the addition of guava byproduct in the diets did not change the intestinal epithelium of the initial portions of the small intestine. This may be due to the absence of oxidative challenge, since the antioxidant additives are more significant to prevent oxidative losses.

The inclusion of guava byproduct in broiler diet resulted in lower crypt depth and linear increase in villus: crypt ratio. According to Kuzmuk et al. (2005), the villus height and the crypt depth are considered indicators of good development of the intestine and, under normal conditions, present a higher villus:crypt ratio, indicating that there was adequate rate of cell renewal and greater absorptive capacity.

According to Macari and Maiorka (2000), the absorption processes are dependent on mechanisms that occur in the intestinal mucosa. Trophic agents that stimulate mitotic process in the crypt-villus region stimulate the development of the mucosa; as a result, the number of cells and villus size are increased. Thus, a trophic agent provides an increase for DNA quantity because it increases the number of cells. These agents are related to ingestion and digestion of food, as well as to the chemical properties of nutrients present in the intestinal lumen.

Thus, this effect may be due to the presence of phenolic compounds in guava, giving it antioxidant properties in scavenging free radicals and suppressing the spread chain of oxidative process (Iha et al., 2008); in addition, the trophic agent promotes intestinal maturity and integrity (Yi et al., 2005). Since the higher the development of villus and crypt and its ratio, the greater the surface absorption of nutrients, better will be the broiler performance. The mucosal damage may increase the need for maintenance, providing smaller quantities of nutrients necessary for the growth of animals (Dibner and Richards, 2004).

Some studies indicate that compounds of secondary metabolism of plants used as additives can beneficially help the intestinal epithelium (Silva et al., 2010; Bona et al., 2012; Amad et al., 2013; Hashemipour et al., 2013).

The final pH of the meat, after 24 h, is determining to the quality of meat because it is related to protein and meat pigments. Therefore, the value that stabilizes influences the color parameters, water holding capacity, tenderness, loss of weight by cooking, juiciness, and microbiological stability (Fletcher, 2002).

The pH of the breast, with the addition of guava byproduct, showed results from 5.90 to 5.96, with no significant difference. These data are within the medium pH for chicken breast meat, which are from 5.70 to 5.96, as described by Mendes (2001) and Van Laack et al. (2000). If the pH is greater than 6.2 after 24 h, the chicken meat will present high water retention, which implies a short conservation time and the establishment of dark color, characterizing the DFD (dark, firm, and dry) meat. If the pH is below 5.8 in less than 4 h, there will be the PSE (pale, soft, and exudative) meat, characterized by poor water retention and the pale and soft appearance (Bridi et al., 2012).

A higher tendency to the yellow (higher b* in relation to a* values) could be observed as Rodrigues et al. (2008), strengthening the muscle characteristics of the broiler breast compared with others such as sheep meat, for example, in which L* = 38,00; a* = 12 to 15; and b* = 3 to 5 (Souza et al., 2004).

In general, the pH of the thigh meat was higher than the pH of the breast, possibly because of differences between the types of muscle fibers of each one. The dark color of the thigh and over thigh meat suggests a greater amount of type I fibers, which are aerobic; therefore, it has a lower glycolytic potential and its metabolism results in less glycogen and decreased production of lactic acid in the transformation of muscle into meat (Erickson, 2006). According to Castellini et al. (2002), the means for thigh pH are between 6.02 and 6.25, which were found in this study.

Unlike the breast, the thigh meat was redder, corroborating Oliveira et al. (2015). According to Faria et al. (2009) and Warris (2010), there is a variation in the pH of the meat from muscles in different anatomical locations. The muscles involved in locomotor activity, such as the thigh and over thigh, for their normal biological activities (movement), generally have lower reserves of glycogen in the myocyte for post-mortem glycolysis. Thus, in these muscles, there is often less extensive acidification and higher pH values within 24 h. In addition, in regions of muscles associated with locomotion, there is greater metabolic intensity and greater need for myoglobin pigment, associated with the intracellular transport of oxygen (Warris, 2010). It is noteworthy that the myoglobin is one of the most important chromogenic pigments of meat.

There was a quadratic effect of supplementation of guava byproduct on the TBARS value of the thigh meat, that is, with increasing inclusion of byproduct, the TBARS value was reduced to 0.72%, indicating greater lipid stability of thigh meat in the inclusion value (Figure 1). At 21 days of age, the antioxidant effect of the guava byproduct on the TBARS parameter can be verified, which means that this effect can probably act at 42 days of age at the slaughter stage.

Figure 1
Thiobarbituric reactive substances (TBARS) resulting from lipid oxidation of the thigh meat sample of 21-day-old broilers fed guava byproduct.

The higher TBARS values found in the thigh meat compared with the breast meat can be explained by differences in metabolism (dark muscle) and composition. The thigh meat, or dark muscles, consists predominantly of oxidative fibers and the breast meat (white muscle) consists predominantly of glycolytic fibers (Banks, 1992). Ramos et al. (2012) stated that the muscles of increased activity are the possessors of higher iron levels. It can be inferred that the richest muscles in myoglobin (thigh meat) are more susceptible to lipid oxidation (Kathirvel and Richards, 2012). Besides having a different kind of muscle, the thigh meat is also richer in lipids than the breast meat.

The results that show a lower lipid oxidation of the thigh meat, for the treatments with guava byproduct, can be attributed to the antioxidant effect of phenolic compounds such as flavonoids, as disclosed by Sebastián et al. (2003) for pork. According to Gatellier et al. (2007), chicken meat has low levels of natural antioxidants such as vitamin E, being particularly prone to lipid oxidation and, therefore, the inclusion of guava byproduct in broiler feed can be an alternative to avoid such a problem.

Another important factor to be considered is the pH of the thigh meat. Although there was no significant difference between the values of experimental treatments, it can be seen that the treatment with the guava byproduct obtained the lowest values. This may be one of the factors that led to a lower lipid oxidation in the meat, which, among other factors, is linked to the development of pathogenic microorganisms, as they have their growth impaired in means with lower pH values.

Conclusions

Guava byproduct can be used as an alternative antioxidant additive in broiler diet at an early stage because it does not depress the productive performance and improves thigh meat quality of broilers.

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Publication Dates

• Publication in this collection
  2018

History

• Received
  22 Sept 2016
• Accepted
  22 May 2017