Prevention of amphotericin B nephrotoxicity through use of phytotherapeutic medication* * Extracted from the dissertation entitled “Efeito protetor da diosmina e hesperidina na nefrotoxicidade induzida pela anfotericina B”, Escola de Enfermagem, Universidade de São Paulo, 2014.

Fábio dos Santos Schlottfeldt Sheila Marques Fernandes Daniel Malisani Martins Priscilla Cordeiro Cassiane Dezoti da Fonseca Mirian Watanabe Maria de Fatima Fernandes Vattimo About the authors

ABSTRACT

Objective

To evaluate the effect of diosmin and hesperidin flavonoids in the prevention of amphotericin B nephrotoxicity, through an experimental model on rats.

Method

Adult, male Wistar rats were distributed into the following groups: saline; diosmin hesperidin (animals that received 50 mg/kg of diosmin hesperidin, drinking water, for ten days); amphotericin B (animals that received 15 mg/kg/day of amphotericin B through intraperitoneal treatment for five days); amphotericin B+diosmin hesperidin. Renal function, fractional excretion of sodium; potassium and magnesium and oxidative metabolites were evaluated.

Results

Treatment with amphotericin B reduced renal function, as shown by the clearance of creatinine, increased tubular function markers and fractional excretion of sodium, potassium, magnesium and oxidative metabolites. Pre-treatment with diosmin hesperidin ameliorated clearance of creatinine and reduced tubular and oxidative injury.

Conclusion

Administration of amphotericin B resulted in reduction of renal function with tubular injury, and diosmin hesperidin showing an antioxidant protective effect on the kidneys.

Antioxidants; Amphotericin B; Diosmin; Hesperidin; Nursing Care; Patient Safety

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E-mail: reeusp@usp.br