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Scientia Agricola, Volume: 80, Published: 2023
  • Monitoring performance indicators of mechanized agricultural operations through a systemic method Agricultural Engineering

    Peloia, Paulo Rodrigues; Milan, Marcos; Romanelli, Thiago Libório; Gimenez, Leandro Maria

    Abstract in English:

    ABSTRACT: Key performance indicators (KPI) are essential to decision-making in an organization, but the approach to analysis and composition used in the formulation of the KPIs can lead to errors. Analysis based only on averages does not allow for discriminating between variations that are natural to the process or special cases which require investigation. The use of control charts can identify this differentiation. However, when several charts are presented encompassing different measurement units and scales, systemic interpretation can be impaired. To assist in this interpretation, this research study aimed at proposing a method to facilitate the analysis of control charts when multiple indicators are employed in the monitoring of agricultural operations. Based on the data obtained over 26 weeks from a mechanized sugarcane (Saccharum officinarum L.) harvesting front, six indicators were defined and analyzed through individual control charts and, systemically, through a standardized group control chart. Results show that the points identified as being outside the control zone (special causes of variation) according to the standardized group control chart were the same as those identified by the six individual charts, which demonstrates the potential of this method to summarize the information with no loss of quality of analysis.
  • Wort disinfection treatment with electron beam for bioethanol production Agricultural Engineering

    Calegari, Rubens Perez; Silva, Eric Alberto da; Silva, Ana Paula Maria da; Gomes, Marcelo Pego; Mota, Layna Amorim; Arthur, Valter; Baptista, Antonio Sampaio

    Abstract in English:

    ABSTRACT Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort from sugarcane (Saccharum officinarum L.) molasses and investigate the bioethanol fermentation. Four treatments (T0 – T3) were carried out using ionizing doses of radiation through the electron accelerator: 0 (control), 10, 20, and 40 kGy. Total mesophiles, total bacteria, sucrose, glucose, fructose, phenolics, flavonoids, hydroxymethylfurfural (5-HMF), and Furfural were measured. An alcoholic fermentation assay was performed after the irradiation process. The irradiated treatments showed no inversion of sugars and formation of the inhibitory by-products flavonoids, furfural and 5-HMF, except for the phenolic compounds. The lower dose tested (10 kGy) reduced more than 99.9 % of the total mesophiles and more than 99.99 % of the total bacteria in the substrate. In the fermentation, the irradiated worts presented similar (p > 0.05) yields (92, 93, and 94 %) and ethanol productivity levels (0.89, 0.88, and 0.87 g L–1 h–1, for T1, T2, and T3 respectively). However, all treatments presented higher yields and productivity (p < 0.05) when compared to the control (88 % and 0.85 g L–1 h–1), highlighting the possible use of e-beam in wort fermentation at a lower dose (10 kGy). This allows reduction in losses caused by microbial contamination, besides increasing fermentation yield and productivity with lower energy consumption.
  • Soybean extracts can improve plant development Agricultural Microbiology

    May, André; Silva, Evandro Henrique Figueiredo Moura da; Vieira Junior, Nilson Aparecido; Vilela, Elke Simoni Dias; Santos, Michelli de Souza dos; Coelho, Luciana Fontes; Pedrinho, Alexandre; Batista, Bruna Durante; Viana, Ronaldo da Silva

    Abstract in English:

    ABSTRACT: Microbial biodiversity of an environment can contribute to plant growth and increase crop yield. Plant extracts from soybean (Glycine max (L.) Merrill) were investigated on soybean plants grown after inoculation with these extracts. Soil samples were collected from two important Brazilian soybean-growing regions to produce the extracts used in the experiments. The extracts were produced with material collected from aboveground biomass and rhizosphere of soybean plants cultivated in a controlled greenhouse (phase 1). The extracts produced in phase 1 were applied in a sequential experiment (phase 2). Phase 2 was conducted to examine the plant microbiome after the microbial alteration process in the greenhouse through seed inoculation with the extracts produced previously. Samples of aboveground biomass were collected to determine root dry matter and crop yield. Bacterial 16S rRNA sequences were processed to determine the final microbial content of soybean. The inoculated treatments had lower species diversity; however, the phyla Firmicutes and Bacteroidetes were more abundant in the treatments than in the non-inoculated treatment. The soybean plant stem in the inoculated treatment also had a positive response to enrichment of the bacterial classes Betaproteobacteria, Bacilli and Flavobacteria. Inoculation affected the microbial composition of soybean plants. The alteration of microbiome changes revealed differences for crop yield between the inoculated and non-inoculated treatments, with up to 93.5 % higher crop yields per plant according to the extract applied.
  • Enzymatic stoichiometry in tropical soil under pure and mixed plantations of eucalyptus with N2-fixing trees Agricultural Microbiology

    Pereira, Arthur Prudêncio de Araujo; Araujo, Ademir Sérgio Ferreira; Santana, Maiele Cintra; Lima, Antonio Yan Viana; Araujo, Victor Lucas Vieira Prudêncio de; Verma, Jay Prakash; Cardoso, Elke Jurandy Bran Nogueira

    Abstract in English:

    ABSTRACT: Soil enzymes play a fundamental role in nutrient cycling in forest systems. The stoichiometry of C, N, and P–acquiring enzymes has been used to indicate nutrient limitation in the soil. However, the enzymatic stoichiometry remains poorly understood in pure and mixed eucalypt plantations. Thus, this study aims to assess the activity of enzymes in the soil to address the hypothesis that the introduction of N2-fixing trees could influence the enzymatic stoichiometry on C, N, and P cycling. The activity of β-glucosidase (BG), urease (U), and acid phosphatase (AP) was assessed in soil (0-20 cm depth) of pure Eucalyptus grandis without (E) and with N fertilization (E+N), and a mixed system with E. grandis and Acacia mangium (E+A), and a pure A. mangium (A) plantation at 27 and 39 months after planting. The activities of BG/U, BG/AP, and U/AP were used to calculate the enzyme C/N, C/P, and N/P ratios, respectively. Rates of N–acquiring enzymes were higher in E and E+N, while soil microorganisms invested in P–acquiring enzymes in A and E+A. The vector length and angle demonstrated that C demand by microorganisms does not change in relation to N and P, regardless of the treatment. However, N demand decreased in relation to P in A and E+A (mainly at 27 months). Our results suggest that enzymes activity in pure eucalypt systems is limited for their soil-litter nutrient contents. At the same time while acacia and mixed plantation seem to invest in P–acquiring enzymes to improve biological N2 fixation promoted by diazotrophic bacteria associated to acacia.
  • Optimization of oxalic acid production by fungi for biotechnological solubilization of rock phosphate Agricultural Microbiology

    Amaro, Jeniffer Kelly Cortes; Xavier, Laura Vieira; Ribeiro, Michelle Miranda Araújo de Carvalho; Vieira, Bruno Sérgio; Mendes, Gilberto de Oliveira

    Abstract in English:

    ABSTRACT: This study describes a biotechnological strategy for producing and applying oxalic acid to solubilize phosphorus (P) from rock phosphate (RP). We evaluated six fungal species (Aspergillus niger FS1, Penicillium islandicum FS41, Pleurotus ostreatus PO1, Rhizoctonia solani Rhiz555, Sclerotium rolfsii Sr25, and Sclerotinia sclerotiorum Scl134) and three culture media (potato dextrose broth, Tsao and Strasser media) to maximize oxalic acid production. Among the fungal isolates tested and culture media, S. rolfsii Sr25 and Tsao medium showed efficient oxalic acid production. Tsao medium was optimized following a response surface methodology after initial screening of factors affecting RP solubilization. The optimized concentrations were 1 g L–1 NaNO3, 100 g L–1 glucose, 2 g L–1 KH2PO4, 4.5 g L–1 yeast extract, and 25 mg L–1 MgSO4·7H2O used for 20 days of incubation. Under these conditions, 71 mmol L–1 oxalic acid was obtained, representing a three-fold increase over production under non-optimized conditions (20 mmol L–1). Under optimized conditions, oxalic acid produced by S. rolfsii Sr25 reacted with low-solubility RP and solubilized 100 % of the P contained in ore. Thus, using S. rolfsii Sr25 to produce oxalic acid seems a promising biotechnological alternative for P solubilization from RP.
  • Coffee crops adaptation to climate change in agroforestry systems with rubber trees in southern Brazil Agrometeorology

    Zaro, Geovanna Cristina; Caramori, Paulo Henrique; Wrege, Marcos Silveira; Caldana, Nathan Felipe da Silva; Virgens Filho, Jorim Sousa das; Morais, Heverly; Yada Junior, George Mitsuo; Caramori, Daniel Campos

    Abstract in English:

    ABSTRACT: Adaptation to climate change is a strategy for crops to cope with the scenario of rising temperatures worldwide. In the case of Coffea arabica L., the use of agroforestry systems (AFS) with woody species is a promising practice to reduce excessive heat during the day. This study aimed to 1) evaluate air temperature changes that occur in an AFS of coffee and double alleys of rubber trees (Hevea brasiliensis Müell. Arg.) and 2) carry out an analysis of future warming scenarios by comparing the cultivation of Arabic coffee in full sun and in an AFS of double alleys of rubber trees. The microclimatic variables were measured between two rows of coffee trees at 1.0 m of height from June 2016 to June 2018. The results indicate that the AFS with double alleys of rubber trees spaced 16 m apart had an average temperature reduction from 1.4 to 2.5 °C from 10h00 to 16h00. The study also simulated temperature increases of 1.7, 2.6, 3.1, and 4.8 °C from 2018 to 2099, according to scenarios predicted by the Intergovernmental Panel on Climate Change (IPCC), and the impact in coffee production in Paraná State, Brazil. Using the climatic generator PGECLIMA_R, simulations suggest a progressive reduction of traditional areas suitable for open-grown coffee in the state. Production conditions can be maintained through the AFS, since the systems attenuate mean temperatures by 1-2 °C. We conclude that the AFS of coffee and rubber trees contribute to coffee crop adaptations to a future warmer environment.
  • Improvement of reference crop evapotranspiration estimates using limited data for the Brazilian Cerrado Agrometeorology

    Althoff, Daniel; Rodrigues, Lineu Neiva

    Abstract in English:

    ABSTRACT Evapotranspiration (ET) is a key component of the hydrological cycle. Therefore, adequately estimating it is crucial to improving water resource planning and management.One of the most affordable methods of estimating ET is first to estimate reference crop evapotranspiration (ETo) and later associate it to crop and soil coefficients. The FAO Kc-ETo approach can be used only when ETo is computed with the FAO Penman-Monteith equation. However, low data availability may restict the equations used to estimate ETo. In this study, we assess and calibrate common methods used to estimate ETo under such conditions of limited data availability. Based on the annual calibration, the Makkink (NSE = 0.85) outperformed the Priestley-Taylor (NSE = 0.73), Hargreaves-Samani (NSE = 0.56), and Penman-Monteith temperature approach (NSE = 0.58). The seasonal calibration of parameters showed no significant improvement to the methods assessed (ΔNSE ≤ 0.01), except for the Priestley-Taylor (ΔNSE = 0.06). The performance of temperature-based equations was particularly limited due to the performance of the equation adopted to estimate global solar radiation. Thus, improving the representation of global solar radiation for limited data availability can also play a key role in improving ETo prediction.
  • Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage Animal Science And Pastures

    Amorim, Jaíne Santos; Gois, Glayciane Costa; Silva, Altiery Felix e; Santos, Milenna Alves dos; Figuêiredo, Priscila Izidro de; Miranda, Juliana Oliveira de; Rodrigues, Rafael Torres de Souza; Araújo, Gherman Garcia Leal de; Voltolini, Tadeu Vinhas

    Abstract in English:

    ABSTRACT: Pornunça (Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay (Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter – DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg−1 DM and 76.78 mg kg−1 DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.
  • Apparent calcium retention and digestibility coefficients of limestone with different particle sizes in laying hens Animal Science And Pastures

    Diana, Thiago Ferreira; Calderano, Arele Arlindo; Rostagno, Horácio Santiago; Marques, Maria Rita de Lima; Tavernari, Fernando de Castro; Veroneze, Renata; Albino, Luiz Fernando Teixeira

    Abstract in English:

    ABSTRACT Apparent calcium (Ca) retention and digestibility coefficients are affected by limestone particle size in the diet of laying hens. This study aimed to determine the apparent retention and digestibility coefficients of Ca in limestone of different particle sizes in laying hens. The study comprised 288 Lohmann Brown laying hens (50 weeks of age; 1,964 ± 98 g) distributed in a completely randomized experimental design with a 3 × 2 factorial arrangement [three Ca concentrations (10, 20, and 30 g kg–1) × two limestone particle sizes (480 and 1,978 µm)] with eight repetitions per treatment and six birds per experimental unit. The experiment included five days for adaptation and five days for total excreta collection. All birds were slaughtered at the end of the ten days to collect the ileal contents. The total or ileal Ca content was plotted against the Ca of diets concentration using linear regression analysis. The regression line slope represented the apparent retention (CaR) and digestibility coefficients of Ca (CaD) in limestone. There was interaction between Ca concentration in the diet and limestone granulometry on CaD (p = 0.001) and CaR (p < 0.001). The CaD and CaR of fine- and coarse-grained limestone increased linearly with increasing Ca concentrations in the diet. The apparent digestibility coefficients estimated for laying hens fed with fine-grained and coarse-grained limestone were 0.72 and 0.35, respectively. The apparent retention coefficients estimated for laying hens fed fine-grained and coarse-grained limestone were 0.96 and 0.47, respectively.
  • Meta-analysis of the experimental coefficient of variation in wheat using the Bayesian and Frequentist approaches Biometry, Modeling And Statistics

    Nardino, Maicon; Silva, Fabyano Fonseca e; Olivoto, Tiago; Barros, Willian Silva; Carvalho, Chainheny Gomes de; Signorini, Victor Silva; Mezzomo, Henrique Caletti; Casagrande, Cleiton Renato

    Abstract in English:

    ABSTRACT A large set of variables is assessed for progeny selection in a plant-breeding program and other agronomic fields. The meta-analysis of the coefficient of variation (CVe) produces information for researchers and breeders on the experimental quality of trials. This analysis can also be applied in the decision-making process of the experimental plan regarding the experimental design, the number of repetitions, and the treatments and plants/progenies to be measured. In this study, we evaluated the dataset distribution and the descriptive statistics of CVe through the Frequentist and Bayesian approaches, aiming to establish the credibility and confidence intervals. We submitted CVe data of ten wheat (Triticum aestivum L.) traits reported in 1,068 articles published to the Bayesian and Frequentist analyses. Sample data were analyzed via Gamma and normal models. We selected the model with the lowest Akaike Information Criterion (AIC) value, and then we tested three link functions. In the Bayesian analysis, uniform distributions were used as non-informative priors for the Gamma distribution parameters with three ranges of q~U (a,b,). Thus, the prior probability density function was given by: pθ =1β-α, θ ∈ α,β. The Bayesian and Frequentist approaches with the Gamma model presented similar results for CVe; however, the range Bayesian credible intervals was narrower than the Frequentist confidence intervals. Gamma distribution fitted the CVe data better than the normal distribution. The credible and confidence intervals of CVe were successfully applied to wheat traits and could be used as experimental accuracy measurements in other experiments.
  • Assessment of grape volatile composition using fluorescence indices of leaves in vineyards affected by iron chlorosis Crop Science

    García, María-Rosa González; Sánchez, Ramón; Vilanova, Mar; Martín, Pedro

    Abstract in English:

    ABSTRACT: Multiplex 330 (MX) is a portable, non-invasive fluorescence sensor that provides different multiparametric indices that are sensitive to the concentration of chlorophylls, flavonols, and anthocyanins on the leaf surface. This study investigated the use of these indices to assess the variability on free volatile composition of grapes (Vitis vinifera L.) in the field as well as other components of their quality potential in vineyards affected by iron deficiency chlorosis (IDC). Twenty non-irrigated Tempranillo/110 Richter vineyard subzones from non-affected to moderately affected by IDC were monitored in Ribera del Duero area (Spain) during two seasons. The results indicated that MX can characterize the spatial variation of leaf pigment concentrations, agronomic performance, and grape quality in vineyards affected by IDC. The MX indices measured at the leaf level close to harvest had better predictive values for the concentrations of free alcohols, volatile acids, C13-norisoprenoids, esters and acetates in the must than vine vigor, size or degree of technological maturity of the grapes. Our study demonstrates that the MX indices to estimate leaf pigment concentrations can be helpful to assess the technological maturity and free volatile composition of wine grapes in vineyards affected by IDC; nevertheless, the efficacy of the indexes may vary according to the year season.
  • Performance of ‘BRS Magna’ vines grown under different training systems, rootstocks and production cycles Crop Science

    Leão, Patrícia Coelho de Souza; Cunha, Marcos Andrei Custódio da; Souza, Edimara Ribeiro de

    Abstract in English:

    ABSTRACT Over the past decade, the business of producing juice from grapes (Vitis labrusca L. and hybrids) has been expanding in response to increasing consumption encouraged by campaigns promoting the benefits grape juice can have on health. This study aimed to determine the effect of the production cycle, vine training system, and rootstock on the yield and physical and physical-chemical characteristics of ‘BRS Magna’ grapes grown in the Vale do Submédio São Francisco in the northeastern region of Brazil. The study was conducted in Petrolina, Pernambuco, over eight production cycles (2017 to 2020). The grapevines were trained in three vine training trellis systems: espalier, lyre, and pergola; and grafted onto two rootstocks: IAC 572 and IAC 766. The production cycle, training system, and rootstocks affected the yield components, vigor, and physical characteristics of the BRS Magna grapes. The combination of the pergola training system and rootstock IAC 766 stood out from the others, providing mean yields estimated at 26 t ha –1 per production cycle. The soluble solids content (SS), titratable acidity (TA), and SS TA –1 ratio were affected neither by the training system nor the rootstock, and higher values for SS and lower values for TA were obtained in the first, second, fourth and eighth production cycles. Thus, the pergola training system and rootstock IAC 766 are recommended for promoting yield gains in the ‘BRS Magna’ grape in the Vale do Submédio São Francisco.
  • Population dynamics, vertical distribution and damage characterization of burrower bug in peanut Entomology

    Michelotto, Marcos Doniseti; Bolonhezi, Denizart; Freitas, Rogério Soares de; Santos, João Francisco dos; Godoy, Ignácio José; Schwertner, Cristiano Feldens

    Abstract in English:

    ABSTRACT: Although the burrower bug (Cyrtomenus mirabilis) is considered a vitalsoil pest in peanut crops, Arachis hypogaea L., in South America, there is little information on its occurrence and damage characterization. This study aimed to evaluate the vertical distribution and fluctuation of the burrower bug in the soil and the damage this species causes in peanuts. Two peanut cultivars (IAC OL3 and IAC 503) were evaluated in three locations in the state of São Paulo: Pindorama, Ribeirão Preto and Votuporanga, in the 2017/18 and 2018/19 harvests. Trenches were dug 0.5 m long, 0.3 m wide and 0.3 m deep, along plant lines at four spots on each sampling date, and stratified in layers 10 cm deep. Plant samples (0.5 m) were collected and evaluated for number of pods and percentage of kernels with symptoms of insect damage. More than 85 % of the C. mirabilis population was found in depths of up to 10 cm, especially after plant fructification, and an increase in nymphs from 100 days after sowing (DAS) was seen when an increased number of maturing pods was observed. The occurrence of nymphs and adults of C. mirabilis and their damage to peanut kernels was similar in both cultivars (IAC OL3 and IAC 503), when these were harvested according to their developmental cycles.
  • Estimating the relationship of sugarcane borer larvae and crop damage based on adult captures and climate variables Entomology

    Assis, Henrico Luís Bizão de; Paiva, Paulo Eduardo Branco; Dinardo-Miranda, Leila Luci; Yamamoto, Pedro Takao

    Abstract in English:

    ABSTRACT: Sugarcane borer Diatraea saccharalis (F.) is the primary sugarcane pest in Brazil. To estimate the relationship between larvae in sugarcane stalks and captures of male adults of D. saccharalis, we collected samples weekly: (1) adults with one delta trap with three virgin females and three female pupae and (2) larvae in 120 stalks per plot of 12.6 hectares (355 × 355 m). The study was conducted in two sites with five plots each, in the municipalities of Nova Ponte and Tupaciguara, Minas Gerais State, Brazil, from July 2016 to May 2017. Relationships between (1) males trapped per week and the number of larvae outside of stalks (LOS) were estimated and (2) we evaluated climate variables, namely average temperature, average relative air humidity, hours with relative air humidity below 30 %, rainfall and number of rainy days, and adults and larvae of D. saccharalis. We obtained generalized linear models for LOS in autumn and for larvae inside the stalks (LIS) in spring and autumn and trapped males in both sites. A significant and direct relationship between LIS and males trapped allows predicting larvae density based on captures of males. In addition, plant damage can be estimated based on accumulated captures of males. There was a negative relationship between hours of air humidity < 30 % and larvae outside of stalks. Densities of LIS can be estimated from male captures and by the humidity variables in the trapping week. Nevertheless, the models require validation in the field.
  • Fast-growing forest management to regulate the balance between wood production and water supply Forestry Science

    Cassiano, Carla Cristina; Moreira, Rildo Moreira e; Ferraz, Silvio Frosini de Barros

    Abstract in English:

    ABSTRACT: Increasingly, fast-growing forest plantations are able to support the wood supply but may simultaneously reduce water availability. The trade-off between wood production and water supply is more evident in areas with low water availability, high seasonal variation, or high water demand from local communities. The management regime adopted in forest plantations can either increase or reduce this trade-off. Thus, we assess herein the water and wood supply under different fast-growing forest plantation management regimes to understand how forest management practices can balance the provision of these services. The study was conducted at two catchments with a predominance of fast-growing forest plantations, namely, the mosaic management catchment (MMC) and the intensive management catchment (IMC). Rainfall and streamflow were monitored for three water years. Hydrological indexes were calculated to assess the hydrological regime of both catchments, and make inventories of the forest to assess forest growth rates. MMC had streamflow coefficients, baseflow index and baseflow stability higher than those of IMC. Mean annual wood increment was 32.73 m3 ha-1 yr-1 in MMC, with a mean age of 15 years, and 44.40 m3 ha-1 yr-1 in IMC at coppice in the second year. MMC hydrological indexes remained stable over the period studied, while in IMC the hydrological indexes were affected by climatic variations, mainly in drier years. MMC showed potential for supplying both water and wood. However, in IMC there was a trade-off between wood supply at the expense of the water supply. Thus, the intensity of fast-growing management can be adjusted to achieve a balance between water and wood supply on a catchment scale.
  • Environmental variability in simultaneous selection of common bean for grain quality and mineral concentration traits Genetics And Plant Breeding

    Ribeiro, Nerinéia Dalfollo; Maziero, Sandra Maria

    Abstract in English:

    ABSTRACT: The selection of common bean (Phaseolus vulgaris L.) lines based on grain quality and mineral concentration traits will be more efficient if a minimum number of experiments is established. This study was carried out to determine whether grain quality and mineral concentration traits are significantly affected by the genotype × environment interaction; to estimate heritability and genetic gain in individual and combined experiments; and to select superior common bean lines considering a minimum number of experiments. A total of 17 common bean genotypes were evaluated in four experiments. Grain quality was determined through seven traits, and the concentration of six minerals was analyzed by acid digestion. Statistical analyses were completed using data obtained from both individual (I, II, III and IV) and combined (I and II; I, II and III; and I, II, III and IV) experiments. Except for the potassium concentration, all traits showed a significant genotype × environment interaction effect. Heritability and genetic gain estimates of grain quality and mineral concentration traits varied when the data were obtained from one or more experiments. Genetic gain may be inflated because of the data being based on one or two experiments. Four carioca bean (BRS MG Uai, LP 09-33, LEC 01-16 and Pérola) and four black bean (TB 02-19, CHP 04-239-52, TB 03-11 and IAC Netuno) genotypes were selected for their high grain quality and mineral concentration based on four experiments. Data from at least four experiments should be used to select common bean lines superior in grain quality and mineral concentration traits to increase the efficiency of simultaneous selection.
  • Inhibition of mycelial growth, conidial germination, and Botrytis cinerea Pers.: Fr colonization in begonia with biocompatible products Plant Pathology

    Piermann, Luana; Fujinawa, Miriam Fumiko; Pontes, Nadson de Carvalho; Galvão, José Abrahão Haddad; Bettiol, Wagner

    Abstract in English:

    ABSTRACT: This study evaluated the effects of potassium and sodium carbonate and bicarbonate, Bacillus subtilis (Cohn, 1872) QST-713, Bacillus pumilus (Meyer & Gottheil, 1901) QST-2808, and crude and roasted coffee oils on the inhibition of mycelial growth and conidial germination in Botrytis cinerea Pers.: Fr and the colonization of begonia (Begonia elatior Hort. ex Steud) leaf discs by B. cinerea inoculated before, simultaneously and after with these alternative products. The assays were carried out using the Baladin begonia cultivar. The inhibition of B. cinerea mycelial growth and conidial germination was proportional to increases in the concentration of all the products. The inhibition of conidial germination was directly proportional to the concentrations of B. pumilus QST-2808 and B. subtilis QST-713. Coffee oils were less efficient in inhibiting germination than the other products. The crude and roasted coffee oils, potassium and sodium carbonates and bicarbonates, and B. pumilus and B. subtilis sprayed 24 h before, simultaneously, or 24 h after pathogen inoculation inhibited the colonization of begonia leaf discs by B. cinerea. The positive results for the suppression of B. cinerea by the alternative products tested herein merit scrutiny. There is a pressing need to evaluate these products in the management of gray mold, as the severity of this disease is usually high under favorable conditions in greenhouses.
  • Tomato fruit blotch virus cytopathology strengthens evolutionary links between plant blunerviruses and insect negeviruses Plant Pathology

    Kitajima, Elliot Watanabe; Nakasu, Erich Yukio Tempel; Inoue-Nagata, Alice Kazuko; Salaroli, Renato Barbosa; Ramos-González, Pedro Luis

    Abstract in English:

    ABSTRACT Tomato fruit blotch virus (ToFBV) is a blunervirus that causes blotches on mature tomato (Solanum lycopersicon L.) fruits in Italy and Australia in 2020, and was newly detected in Brazil. A cytological study on pericarp tissues from the blotched areas of infected fruits collected in Brasília, Brazil, revealed characteristic cell alterations. Small and slender bacilliform particles (ca. 25 nm wide × 100 nm long) were found accumulating in the perinuclear space and the lumen of the endoplasmic reticulum of the epidermis, peri- and mesocarp cells. No viroplasm-like inclusion was observed either in the nuclei or in the cytoplasm. Such cell alterations are reminiscent of those described in cultured mosquito cells infected by negeviruses, an unofficial group of insect viruses. Negeviruses and some other arthropod-borne viruses shared a common ancestor in the RdRp gene with kitavirids, including blunerviruses. Although additional detailed studies are required, we show evidence that ToFBV particles are enveloped and bacilliform, and that such similarity in cytopathology seems to support the evolutionary relationship between plant kitavirids and insect negeviruses.
  • Foliar application of graphene oxide, Fe, and Zn on Artemisia dracunculus L. under salinity Plant Physiology And Biochemistry

    Hassanpouraghdam, Mohammad Bagher; Mehrabani, Lamia Vojodi; Kheirollahi, Nahideh; Soltanbeigi, Amir; Khoshmaram, Leila

    Abstract in English:

    ABSTRACT: Salinity is an abiotic stressor that greatly influences crop growth and yield. Scientists are always exploring diverse methods to combat salinity depression. Here, we conducted a greenhouse experiment to study the effects of NaCl salinity (0, 50, and 100 mM) under the foliar spray with zinc-oxide, nano zinc-oxide, iron-chelate, magnetized-Fe, and graphene-oxide on tarragon. The results revealed that the treatment with foliar spray of graphene showed the highest K+/Na+. Salinity of 50 and 100 mM × all foliar applications increased superoxide dismutase activity, whereas 100 mM NaCl raised the malondialdehyde content to its highest level. All salinity levels × Zn foliar spray improved the catalase activity. The foliar spray and salinity experiment exposed to Zn-treated plants attained the highest contents of essential oils. Proline and total phenolics showed the greatest amounts with 50 and 100 mM of NaCl, respectively. The GC/MS analysis revealed 38 compounds in the oil of Artemisia dracunculus L. as estragole (81-91.8 %) was the most dominant constituent. The highest amounts of estragole were found at 50 and 100 mM NaCl foliar sprayed with ZnO. Cis-Ocimene (0.1-6.4 %) was another major constituent with a high variation between the treatments. The highest cis-ocimene content was recorded at 100 mM NaCl × foliar Fe-chelated and in the graphene treatment. Overall, salinity adversely affected the physiological responses of tarragon; however, foliar spray with both forms of Fe and Zn partially ameliorated the adverse salinity effects.
  • Do enhanced efficiency potassium sources increase maize yield in soil with high potassium content? Soils And Plant Nutrition

    Ribeiro, Bruno Neves; Roms, Rafael Zoccolaro; Coelho, Anderson Prates; Batista-Silva, Willian; Souza, Juscelio Ramos de; Gissi, Luciano de; Lemos, Leandro Borges

    Abstract in English:

    ABSTRACT: Enhanced efficiency potassium fertilizers can be a management tool that is crucial to crop sustainability in maize (Zea mays L.). However, there is a need for studies aimed at validating the use of these fertilizers in different production environments. This study aimed to evaluate the performance of maize under sources and rates of K through conventional and enhanced efficiency fertilizers in soil with high available K content. The experiment was carried out for two years in an Oxisol (605 g kg–1 of clay) with high K content (6.7 mmolc dm–3). Three sources were used, one conventional (KCl), one obtained by additives sprayed on the fertilizer surface (KCl-C), and one obtained by compacting KCl powder and adding additives (KCl-CC), associated with three K2O rates as top-dressing (50, 100, 150 kg ha–1) and a control without K2O. In all treatments, 48 kg ha–1 of K2O was applied in the sowing furrow. In the first year, maize yield increased linearly for both the KCl and KCl-C sources. The maximum yield (7,967 kg ha–1) for the KCl-CC was obtained at 88 kg ha–1. In the second year, the maximum yields for the KCl (7,553 kg ha–1) and KCl-C (8,166 kg ha–1) were obtained with 20 and 67 kg ha–1 K2O, respectively, while for the KCl-CC maize yield did not change. Enhanced efficiency K sources promote increases in maize yield ranging from 4.3 % to 7.1 %. Top-dressing K fertilization in high-fertility soils is a viable alternative for producers focused on increasing maize yield, mainly when enhanced efficiency sources are used.
  • NO 3 , K + , and chlorophyll index in fertigated grapevines in the semi-arid region of Brazil Soils And Plant Nutrition

    Silva, Alexsandro Oliveira da; Silva, Davi José; Bassoi, Luis Henrique; Chaves, Agnaldo Rodrigues de Melo

    Abstract in English:

    ABSTRACT Evaluating the effects of N and K supply on grapevines ( Vitis vinifera L.) and the techniques for nutritional diagnosis is of great importance for fertigation management of this crop. This study evaluated the effects of N and K fertigation on the soluble concentrations of NO 3 – and K + in the petiole sap and on the leaf chlorophyll index in drip irrigated ‘Syrah’ grapevine (from 17 June 2013 to 25 Nov 2014). The treatments consisted of five levels of N (0, 15, 30, 60 and 120 kg ha –1 ) and K 2 O (0, 15, 30, 60 and 120 kg ha –1 ), combined in an incomplete 5 2 factorial scheme in 13 combinations and arranged in randomized blocks with four replications. We determined NO 3 – and K + concentrations in petiole sap, leaf chlorophyll index, grapevine cluster mass and number per plant, mean grapevine cluster mass, and phenolic composition. High NO 3 – concentrations contributed positively to grapevine yield; however, increased K+ concentrations caused a negative response of sap. For 120 kg N ha –1 rate, NO 3 – in the sap and chlorophyll index showed higher values at the flowering stage, while high values for K + were observed during the grape-ripening stage.
  • In memoriam of Paulo Cesar Sentelhas Obituary

    Romanelli, Thiago Libório; Monteiro, Jose Eduardo Boffino de Almeida
Escola Superior de Agricultura "Luiz de Queiroz" USP/ESALQ - Scientia Agricola, Av. Pádua Dias, 11, 13418-900 Piracicaba SP Brazil, Phone: +55 19 3429-4401 / 3429-4486 - Piracicaba - SP - Brazil
E-mail: scientia@usp.br