Abstract
Whole flour of cowpea grains of the cultivar BRS Tumucumaque biofortified in iron and zinc were processed in a co-rotating twin-screw extruder Clextral HT 25. A central rotational composite design 23, combining temperature (from 86.4 to 153.6 °C), screw rotation speed (from 163.6 to 836.4 rpm) and moisture content (from 16.6% to 23.4%), was used to assess the effects on flour constituents. The three variables significantly affected (p < 0.05) the levels of protein and copper in the extrudates. The reactions of the extrusion process caused a decrease in the levels of proteins and an increase in the levels of copper, zinc and potassium, and these amounts were accentuated as the values of the process variables approached those of the central point region. Extreme conditions at high rotational screw speeds combined with low moisture content reduced the magnesium content, at the opposite end of the values for these two variables, the intensity of the reduction was lower. In the analysis of global desirability, it was found that the extrusion condition at 112.6 °C, 587.4 rpm and 23.4% moisture provided the highest nutrient retention. The levels of iron and zinc remained above 60 and 40 mg kg-1, respectively, in extruded products.
Keywords:
Vigna unguiculata; Extrusion; Response surface methodology; Chemical composition; Mineral elements; Instant flour
Resumo
Farinha integral de grãos de feijão-caupi da cultivar BRS Tumucumaque biofortificados em ferro e zinco foi processada em extrusor de rosca dupla corrotativo Clextral HT 25. Um delineamento composto central rotacional 23, combinando as variáveis independentes: temperatura (86,4 a 153,6 °C), velocidade de rotação do parafuso (163,6 a 836,4 rpm) e teor de umidade (16,6% a 23,4%), foi utilizado para avaliar os efeitos sobre os constituintes da farinha. As três variáveis afetaram significativamente (p < 0,05) os teores de proteína e cobre dos extrusados. As reações do processo de extrusão provocaram diminuição nos teores de proteínas e aumento nos teores de cobre, zinco e potássio, e essas quantidades se acentuaram à medida que os valores das variáveis de processo se aproximaram aos da região do ponto central. Condições extremas de altas velocidades de rotação das roscas combinadas com baixos teores de umidade reduziram o teor de magnésio, enquanto que, no extremo inverso de valores para essas duas variáveis, a intensidade da redução foi menor. Na análise de desejabilidade global, verificou-se que a condição de extrusão a 112,6 °C, 587,4 rpm e 23,4% de umidade proporcionou a maior retenção de nutrientes. Os teores de ferro e zinco permaneceram acima de 60 e 40 mg kg-1, respectivamente, nos produtos extrusados.
Palavras-chave:
Vigna unguiculata; Extrusão; Metodologia de superfície de resposta; Composição química; Elementos minerais; Farinha instantânea
1 Introduction
Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important sources of protein in the diet of the semi-arid tropics of the planet, as it is a species of wide adaptability, rusticity, tolerance to conditions of high temperatures and water deficits. It is the 5th most cultivated pulse in the world (Food and Agriculture Organizations of the United Nations, 2020Food and Agriculture Organizations of the United Nations – FAO. (2020, april 30). FAOSTAT. Retrieved in 2020, July 29, from http://www.fao.org/faostat/en/#data/QC/visualize
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). In 2018, Brazil produced around 738 thousand tons of cowpea, or 25% of the total beans (V. unguiculata + Phaseolus vulgaris) produced in the country (3 million tons) (Empresa Brasileira de Pesquisa Agropecuária, 2020Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA. (2020, march 15). Dados conjunturais da produção de feijão-comum (Phaseolus vulgaris L.) e caupi (Vigna unguiculata (L.) Walp.) no Brasil (1985 a 2018): Área, produção e rendimento. Retrieved in 2020, July 29, from http://www.cnpaf.embrapa.br/socioeconomia/index.htm
http://www.cnpaf.embrapa.br/socioeconomi...
). On the world stage, Brazil ranks 3rd as the world´s largest producer, behind Nigeria (2.6 million tons) and Niger (2.4 million tons), and ahead of Burkina Faso (631 thousand tons) (Food and Agriculture Organizations of the United Nations, 2020Food and Agriculture Organizations of the United Nations – FAO. (2020, april 30). FAOSTAT. Retrieved in 2020, July 29, from http://www.fao.org/faostat/en/#data/QC/visualize
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).
The cowpea provides nutritious grains, containing on average 25% of proteins (Food and Agriculture Organizations of the United Nations, 2016Food and Agriculture Organizations of the United Nations – FAO. (2016, march 15). International year of pulses: Nutritious seeds for a sustainable future. Rome: FAO. Retrieved in 2020, July 29, from http://www.fao.org/pulses-2016/news/news-detail/en/c/337107/
http://www.fao.org/pulses-2016/news/news...
) of high digestibility compared to other pulses (Afoakwa et al., 2006Afoakwa, E. O., Yenyi, S. E., & Sakyi-Dawson, E. (2006). Response surface methodology for optimizing the pre-processing conditions during canning of a newly developed and promising cowpea (Vigna unguiculata) variety. Journal of Food Engineering, 73(4), 346-357. http://dx.doi.org/10.1016/j.jfoodeng.2005.01.037
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), in addition to energy, vitamins, soluble fibers and minerals, which assist in reducing cholesterol and control diabetes, thus improving other aspects related to health (Food and Agriculture Organizations of the United Nations, 2016Food and Agriculture Organizations of the United Nations – FAO. (2016, march 15). International year of pulses: Nutritious seeds for a sustainable future. Rome: FAO. Retrieved in 2020, July 29, from http://www.fao.org/pulses-2016/news/news-detail/en/c/337107/
http://www.fao.org/pulses-2016/news/news...
), as well as presenting low cost of production with sustainability. However, it also contains antinutritional compounds, such as trypsin inhibitors, which can be inactivated by adequate heat treatment, as they are thermolabile (Doblado et al., 2007Doblado, R., Frias, J., & Vidal-Valverde, C. (2007). Changes in vitamin C content and antioxidant capacity of raw and germinated cowpea (Vigna sinensis var. carilla) seeds induced by high pressure treatment. Food Chemistry, 101(3), 918-923. http://dx.doi.org/10.1016/j.foodchem.2006.02.043
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).
Thermoplastic extrusion is one of the processes that exposes raw materials or formulations prepared to high temperatures for a short time, which significantly reduce or eliminate most antinutritional compounds (Batista et al., 2010Batista, K. A., Prudêncio, S. H., Fernandes, K. F. (2010). Changes in the biochemical and functional properties of the extruded hard-to-cook cowpea (Vigna unguiculata L. Walp.). International Journal of Food Science and Technology, 45(4), 794-799; Diouf et al., 2019Diouf, A., Sarr, F., Sene, B., Ndiaye, C., Fall, S. M., & Ayessou, N. C. (2019). Pathways for reducing anti-nutritional factors: Prospects for Vigna unguiculata. Journal of Nutritional Health & Food Science, 7(2), 1-10. http://dx.doi.org/10.15226/jnhfs.2019.001157
http://dx.doi.org/10.15226/jnhfs.2019.00...
; Choton et al., 2020Choton, S., Gupta, N., Bandral, J. D., Amjum, N., & Choudary, A. (2020). Extrusion technology and its application in food processing: A review. The Pharma Innovation Journal, 9(2), 162-168.), and modifies the format or expands the raw material (Akhtar et al., 2015Akhtar, J., Malik, S., Alam, M. A., Student, M. T., & Allahabad, S. (2015). Extrusion technology used for novel foods production. International Journal of Engineering Development and Research, 3, 1-7.), allowing to obtain a diversity of products in a short processing time (Choton et al., 2020Choton, S., Gupta, N., Bandral, J. D., Amjum, N., & Choudary, A. (2020). Extrusion technology and its application in food processing: A review. The Pharma Innovation Journal, 9(2), 162-168.), and has recently been directed for obtaining versions of products with healthy and nutritious characteristics for consumers of all age groups (Jakkanwar et al., 2018Jakkanwar, S. A., Rathod, R. P., & Annapure, U. S. (2018). Development of cowpea-based (Vigna unguiculata) extruded snacks with improved in vitro protein digestibility. International Food Research Journal, 25(2), 804-813.). Ready-to-eat products made exclusively from whole cowpea flour are more nutritious and healthier than those obtained from traditional cereal flours, and can be consumed by individuals with restricted gluten intake, such as: celiac, represented by about 1.4% of the population (Singh et al., 2018Singh, P., Arora, A., Strand, T. A., Leffler, D. A., Catassi, C., Green, P. H., Kelly, C. P., Ahuja, V., & Makharia, G. K. (2018). Global prevalence of celiac disease: Systematic review and meta-analysis. Clinical Gastroenterology and Hepatology, 16(6), 823-836. PMid:29551598. http://dx.doi.org/10.1016/j.cgh.2017.06.037
http://dx.doi.org/10.1016/j.cgh.2017.06....
); those who are sensitive or who choose not to consume gluten, which represent 7 to 14% of the population (Cabrera-Chávez et al., 2017Cabrera-Chávez, F., Dezar, G. V. A., Islas-Zamorano, A. P., Espinoza-Alderete, J. G., Vergara-Jiménez, M., Magaña-Ordorica, D., & Ontiveros, N. (2017). Prevalence of self-reported gluten sensitivity and adherence to a gluten-free diet in argentinian adult population. Nutrients, 9(1), 81. PMid:28117706. http://dx.doi.org/10.3390/nu9010081
http://dx.doi.org/10.3390/nu9010081...
).
However, for some bean cultivars, thermoplastic extrusion, in general, has significantly reduced the protein and starch content (Ai et al., 2016Ai, Y., Cichy, K. A., Harte, J. B., Kelly, J. D., & Ng, P. K. W. (2016). Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders. Food Chemistry, 211, 538-545. PMid:27283664. http://dx.doi.org/10.1016/j.foodchem.2016.05.095
http://dx.doi.org/10.1016/j.foodchem.201...
). In a mixture of sorghum and bambara (70:30), the increased extrusion temperature (100 to 130 °C) decreased the protein and lipid content, and maintained or decreased the ash content (Jiddere & Filli, 2016Jiddere, G., & Filli, K. B. (2016). Physicochemical properties of sorghum malta and Bambara groundnut based extrudates. Journal of Food Science Technology Nepal, 9, 55-65. http://dx.doi.org/10.3126/jfstn.v9i0.12075
http://dx.doi.org/10.3126/jfstn.v9i0.120...
). In the production of snacks based on a mixture of rice, pea and carob flour, no change in protein content was observed, however, it could be noted a reduction in lipid content and an increase in carbohydrate content (Arribas et al., 2017Arribas, C., Cabellos, B., Sánchez, C., Cuadrado, C., Guillamón, E., & Pedrosa, M. M. (2017). Impact of extrusión on nutritional composition, dietary fiber and in vitro digestibility of gluten-free snacks based on rice, pea and carob flours blends. Food & Function, 8(10), 3654-3663. PMid:28914314. http://dx.doi.org/10.1039/C7FO00910K
http://dx.doi.org/10.1039/C7FO00910K...
). Mineral elements are unlikely to be lost during water evaporation at the outlet of the matrix (Camire, 1998Camire, M. E. (1998). Chemical changes during extrusion cooking. In F. Shahidi, C. Ho & N. Chuyen (Eds.), Process-induced chemical changes in food (pp. 109-121). New York: Plenum Press. http://dx.doi.org/10.1007/978-1-4899-1925-0_11.
http://dx.doi.org/10.1007/978-1-4899-192...
). However, in some studies, a decrease in the content of some mineral elements has been observed (Anuonye et al., 2010Anuonye, J. C., Onuh, J. O., Egwim, E., & Adeyemo, S. O. (2010). Nutrient and antinutrient composition of extruded acha/soybean blends. Journal of Food Processing and Preservation, 34(s2), 680-691. http://dx.doi.org/10.1111/j.1745-4549.2009.00425.x
http://dx.doi.org/10.1111/j.1745-4549.20...
) or an increase or decrease, depending on the cultivar and the process conditions (Korus et al., 2006Korus, J., Gumul, D., & Achremowicz, B. (2006). The influence of extrusion on chemical composition of dry seeds of bean (Phaseolus vulgaris L.). Electronic Journal of Polish Agricultural Universities, 9(1), 10. Retrieved in 2020, July 29, from http://www.ejpau.media.pl/volume9/issue1/art-10.html
http://www.ejpau.media.pl/volume9/issue1...
). The content of most mineral elements can vary (Peluola-Adeyemi et al., 2014Peluola-Adeyemi, O. A., Idowu, M. A., Sanni, L. O., & Bodunde, G. J. (2014). Effect of some extrusion parameters on the nutriente composition and quality of a snack developed from cocoyam (Xanthomonas sagittifolium) flour. African Journal of Food Science, 8(10), 510-518. http://dx.doi.org/10.5897/AJFS2014.1169205
http://dx.doi.org/10.5897/AJFS2014.11692...
) or not in the thermoplastic extrusion process (Singh et al., 2007Singh, S., Gamlath, S., & Wakeling, L. (2007). Nutritional aspects of food extrusion: A review. International Journal of Food Science & Technology, 42(8), 916-929. http://dx.doi.org/10.1111/j.1365-2621.2006.01309.x
http://dx.doi.org/10.1111/j.1365-2621.20...
). Increases in the levels of calcium, phosphorus, iron and copper have been attributed to water and wear on the extruder cylinder (Singh et al., 2000Singh, D., Chauhan, G. S., Suresh, I., & Tyagi, S. M. (2000). Nutritional quality of extruded snakes developed from composite of rice broken and wheat bran. International Journal of Food Properties, 3(3), 421-431. http://dx.doi.org/10.1080/10942910009524646
http://dx.doi.org/10.1080/10942910009524...
).
If, on the one hand, through food biofortification programs, cowpea cultivars that are able to absorb and accumulate more nutrients in the grains than traditional ones are obtained (Freire Filho, 2011), it is necessary to evaluate the possible nutritional losses that may occur during processing. The contents of the nutrients iron, zinc and proteins have been evaluated in cowpea cultivars. Those whose grains have more than 60 and 40 mg kg-1, respectively, and are considered biofortified in iron and zinc. The observed variation has been from 48.8 to 77.4 mg kg-1 for iron and from 35.6 to 53.7 mg kg-1 for zinc (Freire Filho, 2011). To be considered biofortified in protein, grains must contain about 30% of this nutrient, so the corresponding cooked grain will provide at least 15 g of protein out of the 50 g of the Reference Daily Intake (RDI), but the variation observed in grains has been from 21.5 to 26.8% (Freire Filho, 2011).
Transforming Biofortified Whole Cowpea Flour (BWCF) into crunchy expanded extrudates or instant flours is a convenient option to consume this nutritious and healthy grain, especially important for those who have reduced nutrient absorption capacity due to intestinal injuries (Ribeiro et al., 2017Ribeiro, P. V. M., Santos, A. P., Andreoli, C. S., Ribeiro, S. M. R., Jorge, M. P., & Moreira, A. V. B. (2017). Nutritional status variation and intestinal and extra intestinal symptomatology in patients with celiac disease and non-celiac gluten sensitivity given specialized dietary advice. Revista de Nutrição, 30(1), 57-67. http://dx.doi.org/10.1590/1678-98652017000100006
http://dx.doi.org/10.1590/1678-986520170...
; García-Manzanares & Lucendo, 2011García-Manzanares, A., & Lucendo, A. J. (2011). Nutritional and dietary aspects of celiac disease. Nutrition in Clinical Practice, 26(2), 163-173. PMid:21447770. http://dx.doi.org/10.1177/0884533611399773
http://dx.doi.org/10.1177/08845336113997...
) and for those who choose not to eat foods containing gluten, whose alternatives are less nutritious, since the functionality of gluten is usually replaced by that of starch (Horstmann et al., 2017Horstmann, S. W., Lynch, K. M., & Arendt, E. K. (2017). Starch characteristics linked to gluten-free products. Foods, 6(4), 29. PMid:28383504. http://dx.doi.org/10.3390/foods6040029
http://dx.doi.org/10.3390/foods6040029...
; Rai et al., 2018Rai, S., Kaur, A., & Chopra, C. S. (2018). Gluten-free products for celiac susceptible people. Frontiers in Nutrition, 5, 116. PMid:30619866. http://dx.doi.org/10.3389/fnut.2018.00116
http://dx.doi.org/10.3389/fnut.2018.0011...
).
In this study, it was evaluated how the different conditions of the thermoplastic extrusion process affected the chemical and mineral composition of the raw BWCF of the cultivar BRS Tumucumaque. The information is valuable for optimizing the extrusion process to identify appropriate conditions for obtaining extrudates with greater nutrient retention.
2 Material and methods
2.1 Raw material
The cowpea grains of the biofortified cultivar BRS Tumucumaque produced and cleaned in Sorriso, in the state of Mato Grosso (MT) were crushed in the knife mil (Renard, MFC-180-75-01, São Paulo, SP, Brazil), with a 3 mm diameter opening mesh attached to the outlet, followed by the grinding of a roller mill (Brabender OHG, Quadrumat Senior, Duisburg, North Rhine-Westphalia, Alemanha), resulting in BWCF.
2.2 Thermoplastic extrusion
The raw BWCF was subjected to the thermoplastic extrusion process in the co-rotating twin-screw equipment (Clextral, Evolum HT 25, Firminy, France), each 25 mm diameter screw, 40:1 length:diameter ratio, screw configuration was kept constant (Figure 1c), die containing four circular holes of 3.8 mm in diameter, ten distinct heating zones and a feed rate of 6.79 kg h-1, according to the central rotational composite design 23 (Table 1). The extrusion of the treatments was carried out in random order, after obtaining the extrudates, they were dried in an air circulation oven at 60 °C for 4 hours, followed by packing in polyethylene bags for cooling and grinding (Retsch, MM200, Haan, Germany) for analysis.
(a) Schematic representation of the top view (in mm) of the barrel, screws and front plate assembly (die) of the Clextral Evolum HT25 extruder: A, the barrel; B, the central manifold plate; C, the distributor plate; D, the holder-inserts plate; E, the insert hole; T, module temperature; GG1, feeding module; FER, closed modules; C1F/C2F, conveyor screw of single/double pitch; INO0, interface screw for transition between screws of single and double pitch; and C1FC, reverse screw of single pitch, slotted; (b) Differences in the color shade of extruded flours; (c) Effect of moisture content and process temperature on protein content (%, db) in whole cowpea flours extruded at 500 rpm screws rotation speed.
Variables independent of the thermoplastic extrusion process and levels expressed in coded and real values.
2.3 Chemical analysis
The contents of proteins, lipids, ash and carbohydrates were determined according to the methodology described by AOAC (2010)Association of Official Analytical Chemists – AOAC. (2010). Official methods of analysis (18th ed.). Washington: AOAC.. To determine the levels of Fe, Zn, Cu, Mn, P and K, an extract was initially prepared as following: 0.2 g of samples were deposited in tubes containing 5 mL of nitric acid: perchloric acid (2:1); digested at 200 °C; and then the volume was made up to 20 ml with distilled water. Aliquots of the extract were used to quantify the levels of minerals in the atomic absorption spectrophotometer (GBC, Model 90C, IL, USA). For P content, a 0.2 mL aliquot of the extract was diluted in 8.4 mL of distilled water, and 1 mL of acid ammonia molybdate solution and 4 mL of 2% vitamin C were added, followed by homogenization in Vortex, rest for 5 minutes, followed by reading at 725 nm on a UV-VIS spectrophotometer (Thermo Spectronic, model Genesis 20 4001/4, CA, USA).
2.4 Statistical analysis
The data obtained were analyzed by multiple linear regression (Barros Neto et al., 2010) and response surface graphs were plotted using the Statistical program (StatSoft, Version 10, OK, USA). The significant experimental models (p ≤ 0.05) were submitted to analysis of global desirability, in an equity condition, adopting values of “s” and “t” equal to 1, to identify the process condition that favors the retention of these nutrients.
3 Results and discussions
3.1 Effect of the extrusion process on chemical properties
Tables 2 and 4 show the results of the chemical composition of raw and extruded BWCF under different conditions of temperature and rotation speed of the screws and conditioning moisture of the samples. The protein content in the raw BWCF was 24.76% (db), whereas that of the extruded BWCF ranged from 23.54% to 24.90% (db) (Table 2). Similar reductions have been observed in other extruded leguminous flours (Ai et al., 2016Ai, Y., Cichy, K. A., Harte, J. B., Kelly, J. D., & Ng, P. K. W. (2016). Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders. Food Chemistry, 211, 538-545. PMid:27283664. http://dx.doi.org/10.1016/j.foodchem.2016.05.095
http://dx.doi.org/10.1016/j.foodchem.201...
; Cardoso-Santiago & Arêas, 2001Cardoso-Santiago, R. A., & Arêas, J. A. G. (2001). Nutritional evaluation of snacks obtained from chickpea and bovine lung blends. Food Chemistry, 74(1), 35-40. http://dx.doi.org/10.1016/S0308-8146(00)00335-6
http://dx.doi.org/10.1016/S0308-8146(00)...
), extruded cereal flour (Razzaq et al., 2012Razzaq, M. R., Anjum, F. M., & Khan, M. I. (2012). Effect of extruder variables on chemical characteristics of maize (Zea mays L.) extrudates. Pakistan Journal of Food Sciences, 22(2), 108-116.) and flour mixes (Anuonye et al., 2010Anuonye, J. C., Onuh, J. O., Egwim, E., & Adeyemo, S. O. (2010). Nutrient and antinutrient composition of extruded acha/soybean blends. Journal of Food Processing and Preservation, 34(s2), 680-691. http://dx.doi.org/10.1111/j.1745-4549.2009.00425.x
http://dx.doi.org/10.1111/j.1745-4549.20...
).
Effect of extrusion variables on the proximate composition (dry basis (db) of raw and extruded BWCF.
Iron (Fe), zinc (Zn), magnesium (Mg), copper (Cu), manganese (Mn), phosphorus (P) and potassium (K) in raw and extruded BWCF.
For the protein content of the extruded BWCF, the model obtained was significant (p = 0.008) with a good fit to the observed data (r2 = 0.85) (Table 3). The lowest protein contents were quantified close to the central point region and as the values of the extrusion parameters moved away from the process conditions of that region, there was less reduction in the protein content (Figure 1b). The milder conditions of the extrusion process were associated with higher conditioning moisture content, lower temperature and speed of rotation that preserved the BWCF protein content.
Coefficient values estimated by multivariate regression for proteins, lipids, ash and carbohydrates.
In compound flour (amaranth, beans, peanuts and pumpkin), it has been reported that the extrusion temperature and moisture content had a linear and negative interaction effect on protein content (Akande et al., 2017Akande, O. A., Nakimbugwe, D., & Mukisa, I. M. (2017). Optimization of extrusion conditions for the production of instant grain amaranth-based porridge flour. Food Science & Nutrition, 5(6), 1205-1214. PMid:29188049. http://dx.doi.org/10.1002/fsn3.513
http://dx.doi.org/10.1002/fsn3.513...
). The reduction in protein content has been attributed to denaturation (Peluola-Adeyemi et al., 2014Peluola-Adeyemi, O. A., Idowu, M. A., Sanni, L. O., & Bodunde, G. J. (2014). Effect of some extrusion parameters on the nutriente composition and quality of a snack developed from cocoyam (Xanthomonas sagittifolium) flour. African Journal of Food Science, 8(10), 510-518. http://dx.doi.org/10.5897/AJFS2014.1169205
http://dx.doi.org/10.5897/AJFS2014.11692...
), loss of some amino acids and nitrogen compounds on heating (Rehman & Shah, 2005Rehman, Z., & Shah, W. H. (2005). Thermal heat processing effects on antinutrients, protein and starch digestibility of food legumes. Food Chemistry, 91(2), 327-331. http://dx.doi.org/10.1016/j.foodchem.2004.06.019
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), and the degradation of lysine at high temperatures because of its two available reactive amino groups (Singh et al., 2007Singh, S., Gamlath, S., & Wakeling, L. (2007). Nutritional aspects of food extrusion: A review. International Journal of Food Science & Technology, 42(8), 916-929. http://dx.doi.org/10.1111/j.1365-2621.2006.01309.x
http://dx.doi.org/10.1111/j.1365-2621.20...
).
One of the main reactions that occurs during the extrusion process is the Maillard reaction, being dependent on the amount of substrate (reducing sugars and reactive amino acids) and process conditions, differences in discernible shades in the extruded BWCF (Figure 1a). Although sensorially desirable due to the development of color and flavor, it negatively affects protein quality by the loss of essential amino acids in this reaction (Bjorck & Asp, 1983Bjorck, I., & Asp, N. G. (1983). The effects of extrusion cooking on nutritional value. Journal of Food Engineering, 2(4), 281-30. http://dx.doi.org/10.1016/0260-8774(83)90016-X; Singh et al., 2000Singh, D., Chauhan, G. S., Suresh, I., & Tyagi, S. M. (2000). Nutritional quality of extruded snakes developed from composite of rice broken and wheat bran. International Journal of Food Properties, 3(3), 421-431. http://dx.doi.org/10.1080/10942910009524646
http://dx.doi.org/10.1080/10942910009524...
, 2007Singh, S., Gamlath, S., & Wakeling, L. (2007). Nutritional aspects of food extrusion: A review. International Journal of Food Science & Technology, 42(8), 916-929. http://dx.doi.org/10.1111/j.1365-2621.2006.01309.x
http://dx.doi.org/10.1111/j.1365-2621.20...
).
The lipid content in the BWCF was 1.98% (db). In the extruded samples, the concentrations of lipids ranged from 0.96% to 1.57% (db) (Table 2). The reduction in lipid content was attributed to partial decomposition and volatilization during extrusion processing (Marzo et al., 2002Marzo, F., Alonso, R., Urdaneta, E., Arricibita, F. J., & Ibáñez, F. (2002). Nutritional quality of extruded kidney bean (Phaseolus vulgaris L. var. Pinto) and its effects on growth and skeletal muscle nitrogen fractions in rats. Journal of Animal Science, 80(4), 875-879. PMid:12002323. http://dx.doi.org/10.2527/2002.804875x
http://dx.doi.org/10.2527/2002.804875x...
) and formation of amylose-lipid complexes and lipid proteins. In most comparisons between the lipid content of raw and extruded bean flours from different cultivars, there was a decrease or no change in content after thermal processing (Korus et al., 2006Korus, J., Gumul, D., & Achremowicz, B. (2006). The influence of extrusion on chemical composition of dry seeds of bean (Phaseolus vulgaris L.). Electronic Journal of Polish Agricultural Universities, 9(1), 10. Retrieved in 2020, July 29, from http://www.ejpau.media.pl/volume9/issue1/art-10.html
http://www.ejpau.media.pl/volume9/issue1...
). In other comparisons between raw and extruded flours, a reduction in lipid content was also observed (Razzaq et al., 2012Razzaq, M. R., Anjum, F. M., & Khan, M. I. (2012). Effect of extruder variables on chemical characteristics of maize (Zea mays L.) extrudates. Pakistan Journal of Food Sciences, 22(2), 108-116.; Anuonye et al., 2010Anuonye, J. C., Onuh, J. O., Egwim, E., & Adeyemo, S. O. (2010). Nutrient and antinutrient composition of extruded acha/soybean blends. Journal of Food Processing and Preservation, 34(s2), 680-691. http://dx.doi.org/10.1111/j.1745-4549.2009.00425.x
http://dx.doi.org/10.1111/j.1745-4549.20...
; Rampersad et al., 2003Rampersad, R., Badrie, N., & Comissiong, E. (2003). Physicochemical and sensory characteristics of flavoured snacks from extruded cassava/pigeon pea flour. Journal of Food Science, 68(1), 363-367. http://dx.doi.org/10.1111/j.1365-2621.2003.tb14166.x
http://dx.doi.org/10.1111/j.1365-2621.20...
; Alonso et al., 2000Alonso, R., Aguirre, A., & Marzo, F. (2000). Effects of extrusion and traditional processing methods on antinutrients and in vitro digestibility of protein and starch in faba and kidney beans. Food Chemistry, 68(2), 159-165. http://dx.doi.org/10.1016/S0308-8146(99)00169-7
http://dx.doi.org/10.1016/S0308-8146(99)...
), being attributed to the formation of complexes between amylose and fatty acids. The model obtained by multivariate regression analysis was not predictive (r2 = 0.39) or significant (p = 0.739) (Table 3).
The carbohydrate content in raw BWCF was 69.73% (db), i.e., this was the lowest quantified value, however, the levels between the samples of the extruded treatments ranged from 70.03 to 71.72% (db) (Table 2).
As the carbohydrate content was calculated by difference, the extruded BWCF treatments that showed large decreases in the protein, lipid and ash content, presented higher carbohydrate content, even higher than raw BWCF (Table 2). The increase in the carbohydrate content after extrusion has been observed in other studies (Cardoso-Santiago & Arêas, 2001Cardoso-Santiago, R. A., & Arêas, J. A. G. (2001). Nutritional evaluation of snacks obtained from chickpea and bovine lung blends. Food Chemistry, 74(1), 35-40. http://dx.doi.org/10.1016/S0308-8146(00)00335-6
http://dx.doi.org/10.1016/S0308-8146(00)...
; Anuonye et al., 2010Anuonye, J. C., Onuh, J. O., Egwim, E., & Adeyemo, S. O. (2010). Nutrient and antinutrient composition of extruded acha/soybean blends. Journal of Food Processing and Preservation, 34(s2), 680-691. http://dx.doi.org/10.1111/j.1745-4549.2009.00425.x
http://dx.doi.org/10.1111/j.1745-4549.20...
), however, the decrease has also been reported (Korus et al., 2006Korus, J., Gumul, D., & Achremowicz, B. (2006). The influence of extrusion on chemical composition of dry seeds of bean (Phaseolus vulgaris L.). Electronic Journal of Polish Agricultural Universities, 9(1), 10. Retrieved in 2020, July 29, from http://www.ejpau.media.pl/volume9/issue1/art-10.html
http://www.ejpau.media.pl/volume9/issue1...
; Alonso et al., 2000Alonso, R., Aguirre, A., & Marzo, F. (2000). Effects of extrusion and traditional processing methods on antinutrients and in vitro digestibility of protein and starch in faba and kidney beans. Food Chemistry, 68(2), 159-165. http://dx.doi.org/10.1016/S0308-8146(99)00169-7
http://dx.doi.org/10.1016/S0308-8146(99)...
). The regression model for the carbohydrate content in WCF extrudates showed a relatively high coefficient of determination (r2 = 0.73), but it was not significant (p = 0.080) (Table 3).
The ash content in the raw BWCF was 3.55% (db), and in the extruded BWCF the concentrations were significantly equal to or higher than the raw BWCF, varying from 3.55% to 4.38% (db) (Table 2). The model had a low determination coefficient (r2 = 0.62) and was not significant (p = 0.231) (Table 3). Some studies have reported an increase in ash content in extruded products (Marzo et al., 2002Marzo, F., Alonso, R., Urdaneta, E., Arricibita, F. J., & Ibáñez, F. (2002). Nutritional quality of extruded kidney bean (Phaseolus vulgaris L. var. Pinto) and its effects on growth and skeletal muscle nitrogen fractions in rats. Journal of Animal Science, 80(4), 875-879. PMid:12002323. http://dx.doi.org/10.2527/2002.804875x
http://dx.doi.org/10.2527/2002.804875x...
; Anuonye et al., 2010Anuonye, J. C., Onuh, J. O., Egwim, E., & Adeyemo, S. O. (2010). Nutrient and antinutrient composition of extruded acha/soybean blends. Journal of Food Processing and Preservation, 34(s2), 680-691. http://dx.doi.org/10.1111/j.1745-4549.2009.00425.x
http://dx.doi.org/10.1111/j.1745-4549.20...
; Pérez-Navarrete et al., 2007Pérez-Navarrete, C., Betancur-Ancona, D., Casotto, M., Carmona, A., & Tovar, J. (2007). Effect of extrusion on protein and starch bioavailability in corn and lima bean flour blends. Archivos Latinoamericanos de Nutricion, 57(3), 278-286. PMid:18271407.) and others decrease (Korus et al., 2006Korus, J., Gumul, D., & Achremowicz, B. (2006). The influence of extrusion on chemical composition of dry seeds of bean (Phaseolus vulgaris L.). Electronic Journal of Polish Agricultural Universities, 9(1), 10. Retrieved in 2020, July 29, from http://www.ejpau.media.pl/volume9/issue1/art-10.html
http://www.ejpau.media.pl/volume9/issue1...
; Cardoso-Santiago & Arêas, 2001Cardoso-Santiago, R. A., & Arêas, J. A. G. (2001). Nutritional evaluation of snacks obtained from chickpea and bovine lung blends. Food Chemistry, 74(1), 35-40. http://dx.doi.org/10.1016/S0308-8146(00)00335-6
http://dx.doi.org/10.1016/S0308-8146(00)...
), and there are reports of an increase in one cultivar and a decrease in another after extrusion (Razzaq et al., 2012Razzaq, M. R., Anjum, F. M., & Khan, M. I. (2012). Effect of extruder variables on chemical characteristics of maize (Zea mays L.) extrudates. Pakistan Journal of Food Sciences, 22(2), 108-116.).
All extruded samples continued to be rich in Fe, as they maintained levels above 60 mg kg-1. There was a variation from 72.32 to 103.09 mg kg-1 for this element among the extruded samples, the raw BWCF showed highest levels (Table 4). The regression model was neither predictive (r2 = 0.65) nor significant (p = 0.191).
The zinc contents ranged from 29.87 to 50.22 mg kg-1 (Table 4). The model presented a good fit to the observed data (r2 = 0.85) and significant (p = 0.009), the model's lack of adjustment was also significant (lack off it = 0.001) (Table 5). According to Henika (1972)Henika, R. G. (1972). Simple and effective system for use with response surface methodology. Cereal Science Today, 17(10), 309-314. whenever the mean square of the experimental error assumes extremely low values, the significance of the tests due to lack of adjustment should be considered irrelevant. In this case, the value was 5.5 times lower than the mean square of the regression, and the significance of the lack of adjustment was disregarded. Thus, the Zn content was influenced by the linear and quadratic effect of the rotation speed of the screws and by the quadratic effect of the temperature (Table 5). It was observed that as the processing conditions moved away from the central point region, the zinc levels decreased (Figure 2a).
Coefficient values estimated by multivariate regression for iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), phosphorus (P), magnesium (Mg) and potassium (K).
Effect of screws rotation speed (rpm) and temperature of the extrusion process (°C) on the content of (a) zinc (mg 100 g-1), (b) magnesium (mg 100 g-1), (c) copper and (d) potassium (g 100 g-1) in BWCF extrudates conditioning at 20% moisture.
Mg levels ranged from 23.72 to 29.98 mg kg-1 (Table 4). The regression model showed a good fit to the observed data (r2 = 0.87) and significant (p = 0.017). It was influenced by the linear and quadratic effects of the thread rotation speed, and its interaction with temperature and moisture (Table 5). In general, the Mg content decreased as the rotation speed of the screws became close to the extreme values (Figure 2b).
The Cu contents ranged from 4.59 to 6.20 mg kg-1, with the raw BWCF showing high levels, in line with the treatments of the central point (Table 4). The model presented a good fit to the observed data (r2 = 0.85) and significant (p = 0.009). The significance of the lack of adjustment was disregarded, following recommendations of Henika (1972)Henika, R. G. (1972). Simple and effective system for use with response surface methodology. Cereal Science Today, 17(10), 309-314.. As conditions in the central point moved away, Cu levels decreased (Figure 2b).
The levels of Mn varied from 9.11 to 10.22 mg kg-1 in the extruded samples (Table 4). The regression model was neither predictive (r2 = 0.69) nor significant (p = 0.125) (Table 5). For P, the variation was from 4.85 to 5.22 g kg-1 (Table 4), and the model was not predictive (r2 = 0.54) nor significant (p = 0.405) (Table5). The K levels among the extruded samples ranged from 13.12 to 16.26 g kg-1, and the raw BWCF presented one of the highest levels, close to the treatments of the central point (Table 4). The model presented a good fit to the observed data (r2 = 0.82) and significant (p = 0.017), being significantly affected by the quadratic effects of the rotation speed of the screws and the moisture content of the flours (Table 5). As the conditions of the extrusion process moved away from the central point, there was a reduction in K levels (Figure 2d).
There is little information about the effect of the extrusion process on the levels of minerals in food. A greater emphasis has been directed to the element Fe, relating the increases in the contents of the extrudates due to the wear of the metal parts during processing, in general, severe processing conditions can transfer the iron element from the extruder to the food due to the wear of the parts (Camire, 1998Camire, M. E. (1998). Chemical changes during extrusion cooking. In F. Shahidi, C. Ho & N. Chuyen (Eds.), Process-induced chemical changes in food (pp. 109-121). New York: Plenum Press. http://dx.doi.org/10.1007/978-1-4899-1925-0_11.
http://dx.doi.org/10.1007/978-1-4899-192...
). The non-uniform hydration in flour particles and abrasive particles can also cause wear of the metal parts (Rokey et al., 2010Rokey, G. J., Plattner, B., & Souza, E. M. (2010). Feed extrusion process description. Revista Brasileira de Zootecnia. 39(Suppl. Spe), 510-518. http://dx.doi.org/10.1590/S1516-35982010001300055.
http://dx.doi.org/10.1590/S1516-35982010...
) or due to deflection of the screws and high rotation speeds (Martin, 2016Martin, C. (2016). Twin screw extruders as continous mixer for termal processing: A technical and historical perspective. American Association of Pharmaceutical Scientists Technology, 17(1), 3-19. PMid:26883259.). The addition of mineral elements can also be attributed to the water used to moisten the raw material (Singh et al., 2000Singh, D., Chauhan, G. S., Suresh, I., & Tyagi, S. M. (2000). Nutritional quality of extruded snakes developed from composite of rice broken and wheat bran. International Journal of Food Properties, 3(3), 421-431. http://dx.doi.org/10.1080/10942910009524646
http://dx.doi.org/10.1080/10942910009524...
), which was not the case, as distilled water was used.
For the same model of mono-screw extruder, the following were published: 1) in extrudates of the mixture of dehulled pigeon pea flour with green banana flour (3:1), conditioned at 25% of moisture, and extruded at 100 °C and 120 rpm, the levels of Fe, Zn, Cu, Mg, Ca, K and Na were higher in relation to the raw mixture (Anuonye et al., 2012Anuonye, J. C., Jigam, A. A., & Ndaceko, G. M. (2012). Effects of extrusion-cooking on the nutrient and anti-nutrient composition of pigeon pea and unripe plantains blends. Journal of Applied Pharmaceutical Science, 2(5), 158-162.); 2) In extrudates made up of dehulled soy flour and green banana pulp flour (3:1), 25% of moisture, 150 °C and 150 rpm, an increase in the content of Fe (69.44%), Ni (28.57%), Se (6.25%) and Na (38.89%), and reductions in the content of Zn (93.57%), Ca (28.29%), Mg (34.57%), Cr (70.56%) and K (51.11%), were observed (Anuonye et al., 2010Anuonye, J. C., Onuh, J. O., Egwim, E., & Adeyemo, S. O. (2010). Nutrient and antinutrient composition of extruded acha/soybean blends. Journal of Food Processing and Preservation, 34(s2), 680-691. http://dx.doi.org/10.1111/j.1745-4549.2009.00425.x
http://dx.doi.org/10.1111/j.1745-4549.20...
); 3) in whole flours of 5 bean cultivars under 4 different conditions (moisture/temperature: 14%/120 °C; 20%/120 °C; 14%/180 °C; 20%/180 °C), it was demonstrated that in relation to the 20 extruded combinations, the raw flours presented Fe contents always higher; for Zn of the 20 combinations, six presented contents equal to or less than the raw BWCF (6/20); for Mg, this ratio was 12/20; Cu 11/20; Mn 2/20; P 7/20; and K 8/20 (Korus et al., 2006Korus, J., Gumul, D., & Achremowicz, B. (2006). The influence of extrusion on chemical composition of dry seeds of bean (Phaseolus vulgaris L.). Electronic Journal of Polish Agricultural Universities, 9(1), 10. Retrieved in 2020, July 29, from http://www.ejpau.media.pl/volume9/issue1/art-10.html
http://www.ejpau.media.pl/volume9/issue1...
).
In a double-screw extruder, 25% of moisture, 100 rpm and at 150 °C, the Fe content in pea flours extrudates was significantly higher than raw flour, but without differences in the levels of Ca, Mg, Cu, Mn, and P (Alonso et al., 2001Alonso, R., Rubio, L., Muzquiz, M., & Marzo, F. (2001). The effect of extrusion cooking on mineral bioavailability in pea and kidney bean seed meals. Animal Feed Science and Technology, 94(1-2), 1-13. http://dx.doi.org/10.1016/S0377-8401(01)00302-9
http://dx.doi.org/10.1016/S0377-8401(01)...
). This same behavior was observed between extruded bean flour and its respective raw flour, processed at 155 °C. This increase in the Fe content in the extrudates was probably related to the wear and tear of metallic parts, mainly of the extruder thread (Alonso et al., 2001Alonso, R., Rubio, L., Muzquiz, M., & Marzo, F. (2001). The effect of extrusion cooking on mineral bioavailability in pea and kidney bean seed meals. Animal Feed Science and Technology, 94(1-2), 1-13. http://dx.doi.org/10.1016/S0377-8401(01)00302-9
http://dx.doi.org/10.1016/S0377-8401(01)...
).
The variations observed in the chemical composition of BWCF should occur similarly in whole flours from non-biofortified cultivars.
3.2 Optimization of process conditions using desirability analysis
Using the significant experimental models, the process conditions that resulted in lower nutrient losses or overall desirability (D) were determined, obtaining a value equal to 0.75 (Figure 3), a relatively high value, being considered acceptable and good, according to the Lazic (2004)Lazic, Z. R. (2004). Design of experiments in chemical engineering: A pratical guide. Weinheim: Wiley-VCH. http://dx.doi.org/10.1002/3527604162.
http://dx.doi.org/10.1002/3527604162...
scale, and the process condition that provided this greater preservation of nutrients was the combination of 112.6 °C, 587.4 rpm and 23.4% of moisture (Figure 3).
Graph of the desirability function for nutrient preservation responses (proteins, zinc, magnesium, copper and phosphorus).
4 Conclusions
The extrusion temperature, screw rotation speed and conditioning moisture significantly affected the protein and copper contents of the Transforming Biofortified Whole Cowpea Flour (BWCF) extrudates. Zinc levels were not influenced by moisture. The magnesium and potassium contents were not affected by the temperature. It was possible to obtain an acceptable and good global desirability for nutrient retention in BWCF extrudates, but it is necessary to reconcile this information with the sensory characteristics of the extrudates.
Acknowledgements
The Brazilian Agricultural Research Corporation (Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) for its financial support, to the EMBRAPA Process Management System (SEG) nº 03.14.01.001.00.00.
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Cite as: Hashimoto, J. M., Sampaio, J. P. M., Franco, L. J. D., Nabeshima, E. H, & Silva, K. J. D. (2021). Optimization of nutrient retention in whole flours extruded from cowpea biofortified grain. Brazilian Journal of Food Technology, 24, e2020185. https://doi.org/10.1590/1981-6723.18520
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Funding: The Brazilian Agricultural Research Corporation (EMBRAPA) for its financial support, to the EMBRAPA Process Management System (SEG) nº 03.14.01.001.00.00.
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Publication Dates
-
Publication in this collection
17 Dec 2021 -
Date of issue
2021
History
-
Received
29 July 2020 -
Accepted
10 Aug 2021