Predominant trait inheritance in wheat with multivariate approach

Herança predominante de caracteres em trigo com enfoque multivariado

Rita Carolina de Melo Marcelo de Carli Toigo Sergio Dias Lannes Nicole Trevisani Thayse Cristine Vieira Pereira Sibila Grigolo Altamir Frederico Guidolin Jefferson Luís Meirelles Coimbra About the authors

ABSTRACT:

The objective of this study was to analize the inheritance of characters in wheat using multivariate analysis of variance. Sixteen genetic constitutions were evaluated between commercial cultivars and progenies F1 and F2. Treatments were arranged in completely randomized blocks, with three replicates. Five characters were evaluated. Multivariate analysis of variance and multivariate contrasts were then performed to test the hypotheses. The significant differences, obtained for comparison between parents and progenies F1, may be evidence of heterosis occurrence. However, only comparisons between progenies P1_P3 (F1) vs. P1_P3 (F2) and P4_P2 (F1) vs. P4_P2 (F2) showed significant differences. Multivariate analysis of variance revealed that inheritance of the characters studied is predominantly of the additive type. It is possible that, this occurred due to the degree of relationship between parents who reunited identical alleles at the same locus, by ancestry, as a result of inbreeding effects. The knowledge of additive inheritance may represent a faster incorporation of resistance or quality characteristics in new cultivars.

Key words:
Triticum aestivum; multivariate contrasts; additivity; inbreeding

RESUMO:

O objetivo deste trabalho foi estudar a herança de caracteres em trigo utilizando análise de variância multivariada. Foram avaliadas dezesseis constituições genéticas, entre cultivares comerciais e progênies F1 e F2. Os tratamentos foram dispostos em blocos completos casualizados, com três repetições. Cinco caracteres foram mensurados. Para testar as hipóteses foi realizada análise de variância multivariada, e posteriormente contrastes multivariados. As diferenças significativas obtidas para as comparações entre os genitores e as progênies F1, podem ser indícios da ocorrência de heterose. No entanto, somente as comparações entre as progênies P1_P3 (F1) vs. P1_P3 (F2) e P4_P2 (F1) vs. P4_P2 (F2) apresentaram diferenças significativas. A análise de variância multivariada revelou que a herança dos caracteres estudados é predominantemente do tipo aditiva. Isto ocorreu, possivelmente pelo grau de parentesco entre os genitores que reuniram alelos idênticos no mesmo loco por ascendência, como resultado dos efeitos da endogamia. O conhecimento da herança aditiva pode representar a incorporação mais rápida de resistência ou de características de qualidade em novas cultivares.

Palavras-chave:
Triticum aestivum; contrastes multivariados; aditividade; endogamia

INTRODUCTION:

The study of genetic inheritance of characters from directed crossings substantially contributes to understand the availability of genetic variability and gains with selection. The inheritance of a particular trait may be predominantly of the additive type or dominance (FEHR, 1987FEHR, W.R. Principles of cultivars development. New York: Macmillan, 1987. 536p.). Quantitatively, heterosis is measured by the difference between the value obtained in generation F1 and the mean value of the parents, being maximum in generation F1. In diploid species, heterozygosis is expected to be reduced to half every self-fertilization generation due to endogamy (WRIGHT, 1950WRIGHT, S. The genetical structure of populations. Annals of Eugenics, v.15, p.323-354, 1950. Available from: http://onlinelibrary.wiley.com/doi/10.1111/j.1469-1809.1949.tb02451.x/pdf>. Accessed: Apr. 24, 2017.
http://onlinelibrary.wiley.com/doi/10.11...
).

Making crossings between contrasting parents is necessary to study the inheritance of a character. In conducting a wheat breeding program by directed hybridization, establishing which parents should be crossed is one of the most important points because there is a large number of genotypes available. Whenever possible, other important traits should be considered while choosing the parents in a given cross, because they are essential for the commercial acceptance of a new cultivar (BENIN et al., 2009BENIN, G. et al. Combining ability of wheat genotypes estimated by multivariate analysis. Pesquisa Agropecuária Brasileira, v.44, n.9, p.1145-1151, 2009. Available from: https://seer.sct.embrapa.br/index.php/pab/article/view/2479/5816>. Accessed: Aug. 20, 2016. doi: 10.1590/S0100-204X2009000900012.
https://seer.sct.embrapa.br/index.php/pa...
).

The information obtained from experiments can be enriched when analyzed in a multivariate model, since selection of genotypes should not be based on only one character (DAWSON et al., 2012DAWSON, J.C. et al. Multi-trait evolution of farmer varieties of bread wheat after cultivation in contrasting organic farming systems in Europe. Genetica, v.140, p.1-17, 2012. Available from: http://dx.doi.org/10.1007/s10709-012-9646-9>. Accessed: Aug. 10, 2016. doi: 10.1007/s10709-012-9646-9.
http://dx.doi.org/10.1007/s10709-012-964...
; YEATER et al., 2015YEATER, K.M. et al. Multivariate analysis: greater insights into complex systems. Agronomy Journal, v.107, n.2, p.799-810, 2015. Available from: http://dx.doi.org/10.2134/agronj14.0017>. Accessed: Sept. 02, 2016. doi: 10.2134/agronj14.0017.
http://dx.doi.org/10.2134/agronj14.0017...
). Multivariate analysis of variance can identify the existing (co)variation between the response variables (HAIR et al., 2007HAIR, J.F.J. et al. Análise multivariada de dados. 6.ed. São Paulo: Bookmam, 2007. 593p.). The possibility of clarifying both relationship and effect of each study variable may be of fundamental importance in the area of biological sciences (CASTRO et al., 2013CASTRO, A.F.N.M. et al. Multivariate analysis for the selection of eucalyptus clones destined for charcoal production. Pesquisa Agropecuária Brasileira , v.48, n.6, p.627-635, 2013. Available from: http://dx.doi.org/10.1590/S0100-204X2013000600008>. Accessed: Aug. 12, 2016. doi: 10.1590/S0100-204X2013000600008.
http://dx.doi.org/10.1590/S0100-204X2013...
; HUANG et al., 2015HUANG, M. et al. Diallel analysis of combining ability and heterosis for yield and yield components in rice by using positive loci. Euphytica, v.205, p.37-50, 2015. Available from: http://dx.doi.org/10.1007/s10681-015-1381-8>. Accessed: Sept. 10, 2016. doi: 10.1007/s10681-015-1381-8.
http://dx.doi.org/10.1007/s10681-015-138...
; MOUSAVIZADEH et al., 2015MOUSAVIZADEH, S.J. et al. Multivariate analysis of edible Asparagus species in Iran by morphological characters. Euphytica , v.206, p.445-457, 2015. Available from: http://dx.doi.org/10.1007/s10681-015-1508-y>. Accessed: Sept. 10, 2016. doi: 10.1007/s10681-015-1508-y.
http://dx.doi.org/10.1007/s10681-015-150...
; SUSO & RÍO, 2015SUSO, M.J.; RÍO, R. del. A crop-pollinator inter-play approach to assessing seed production patterns in faba bean under two pollination environments. Euphytica , v.201, p.231-251, 2015. Available from: http://dx.doi.org/10.1007/s10681-014-1200-7>. Accessed: Sept. 03, 2016. doi: 10.1007/s10681-014-1200-7.
http://dx.doi.org/10.1007/s10681-014-120...
). For example, multivariate analysis in genetic improvement may facilitate the classification and identification of superior genetic constitutions (COIMBRA et al., 2007COIMBRA, J.L.M. et al. Técnicas multivariadas aplicadas ao estudo da fauna do solo: contrastes multivariados e análise canônica discriminante. Revista Ceres , v.54, n.313, p.271-277, 2007. Available from: http://www.redalyc.org/articulo.oa?id=305226813006>. Accessed: Sept. 01, 2016.
http://www.redalyc.org/articulo.oa?id=30...
; SCHMIT et al., 2016SCHMIT, R. et al. Application of multivariate techniques in the evaluation of pure lines of beans. Ciência Rural, v.46, n.9, p.1535-1541, 2016. Available from: http://dx.doi.org/10.1590/0103-8478cr20140329>. Accessed: Sept. 02, 2016. doi: 10.1590/0103-8478cr20140329.
http://dx.doi.org/10.1590/0103-8478cr201...
), as well as help understand the relationship between the characters evaluated thus allowing to understand the type of allele interaction involved in inheritance (BERTAN et al., 2009BERTAN, I. et al. Efeitos da heterose e endogamia em caracteres de importância agronômica em trigo. Revista Ceres, v.56, n.6, p.753-763, 2009. Available from: http://www.redalyc.org/articulo.oa?id=305226942008>. Accessed: Jan. 10, 2017.
http://www.redalyc.org/articulo.oa?id=30...
; SIAHBIDI et al., 2013SIAHBIDI, M.M.P. et al. Evaluation of genetic diversity and interrelationships of agromorphological characters in durum wheat (Triticum durum desf.) lines using multivariate analysis. International Journal of Agriculture, v.3, n.1, p.184-194, 2013. Available from: http://search.proquest.com/openview/d30435836651180c5d0b4b792d1207d3/1?pq-origsite=gscholar&cbl=1596380>. Accessed: Jan. 10, 2017.
http://search.proquest.com/openview/d304...
). Therefore, the objective of this research was to analize the inheritance of characters of agronomic interest in wheat using a multivariate approach.

MATERIAL AND METHODS:

The study was conducted during the winter crop in 2014, at the experimental field, which is located in the Research Center of the State Foundation for Agricultural Research (FEPAGRO), in the city of Vacaria (RS). The region is located in latitude 28º27’S and longitude 50°57’W (height: 950m). The soil is classified as typical Aluminoferric brunolatosol. According to the Koeppen international classification, the climate of the region is humid and subtropical, with mild summers.

Genetic constitutions (16) were evaluated: i) commercial cultivars (4) (‘BRS 331’, ‘Marfim’, ‘CD124’ e ‘BRS Parrudo’); ii) progenies F1 (6), obtained by artificial crosses between cultivars forming a complete diallel, without the reciprocal ones; iii) progenies in generation F2 (6), which were obtained by self-fertilization of progenies F1. The parents (P1: ‘BRS 331’; P2: ‘Marfim’; P3: ‘CD124’; e P4: ‘BRS Parrudo’) were selected for hybridization due to their contrasting characteristics and agronomic performances (Table 1). Treatments were arranged in completely randomized blocks with three replicates. The experimental plots consisted of two 0.2-m spaced 3-m length lines, with 0.1-m spacing between plants in the line.

Table 1
Main characteristics of wheat parents involved in hybridizations. UDESC-IMEGEM, Lages SC, Brazil.

Five characters were measured: (1) days from emergence to silking (DES); (2) plant height (PH; in cm), measure from the soil to the top of the spike, without considering the awns; (3) peduncle length (PL, in cm), distance from the last internode to the base of the spike of the main stem; (4) number of spikes per plant (SPP), counting of spikes that formed grains; and (5) grain mass per plant (GMP, in g). Averages for each character were obtained from all plants of the experimental unit.

The hypotheses on the genetic constitutions were tested by the multivariate analysis of variance (MANOVA). This analysis was obtained by means of the GLM (General Linear Model) procedure of the statistical SAS (SAS Institute, 2009SAS Institute. SAS/STAT: user’s guide version 9.2. Cary, 2009. 60p.) software. Interpretation of the MANOVA analysis indicated the global variation in the data. However, we are interested in the comparison between treatments. For this, multivariate contrast analysis between non-orthogonal means were performed. The comparisons (structure of treatments) were performed as follows: i) average of the parents (P1, P2, P3, and P4) vs. the respective progeny F1; ii) average of progenies F1 vs. average of progenies F2. The contrasts were as follows: C1: Mean of parents P1 and P3 vs. P1_P3 (F1); C2: Mean of parents P2 and P3 vs. P2_P3 (F1); C3: Mean of parents P2 and P1 vs. P2_P1 (F1); C4: Mean of parents P4 and P3 vs. P4_P3 (F1); C5: Mean of parents P4 and P1 vs. P4_P1 (F1); C6: Mean of parents P4 and P2 vs. P4_P2 (F1); C7: P1_P3 (F1) vs. P1_P3 (F2); C8: P2_P3 (F1) vs. P2_P3 (F2); C9: P2_P1 (F1) vs. P2_P1 (F2); C10: P4_P3 (F1) vs. P4_P3 (F2); C11: P4_P1 (F1) vs. P4_P1 (F2); C12: P4_P2 (F1) vs. P4_P2 (F2).

To differentiate between treatments, the canonical coefficients for each contrast were analyzed for the response variables with greater canonical weight. The standardized canonical coefficients (SCC) were interpreted as follows: i) positive values indicated effect of separation between treatments, and characters with higher SCC values showed a greater weight in differentiation; ii) negative values can be interpreted in a similar manner, but with direction contrary to the effect, so that negative values reduced the effect of the response variable under consideration (HAIR et al., 2007HAIR, J.F.J. et al. Análise multivariada de dados. 6.ed. São Paulo: Bookmam, 2007. 593p.).

RESULTS AND DISCUSSION:

Multivariate analysis of variance showed a significant difference for all factors evaluated, showing that populations present genetic variability for the set of characters that were simultaneously evaluated (Table 2). The presence of genetic variability is determinant for the continuity of studies on inheritance. However, notably, understanding of character inheritance seems to be restricted to univariate analyzes (BERTAN et al., 2009BERTAN, I. et al. Efeitos da heterose e endogamia em caracteres de importância agronômica em trigo. Revista Ceres, v.56, n.6, p.753-763, 2009. Available from: http://www.redalyc.org/articulo.oa?id=305226942008>. Accessed: Jan. 10, 2017.
http://www.redalyc.org/articulo.oa?id=30...
; VALENTINI et al., 2011VALENTINI, G. et al. Herança da resistência em feijoeiro à murcha causada por Curtobacterium flaccumfaciens pv. flaccumfaciens. Pesquisa Agropecuária Brasileira , v.46, n.9, p.1045-1052, 2011. Available from: http://dx.doi.org/10.1590/S0100-204X2011000900011>. Accessed: Aug. 28, 2016. doi: 10.1590/S0100-204X2011000900011.
http://dx.doi.org/10.1590/S0100-204X2011...
; CARPENTIERI-PIPOLO et al., 2014CARPENTIERI-PIPOLO, V. et al. Inheritance of late flowering in natural variants of soybean cultivars under short-day conditions. Pesquisa Agropecuária Brasileira , v.49, n.10, p.796-803, 2014. Available from: http://dx.doi.org/10.1590/S0100-204X2014001000006>. Accessed: Sept. 08, 2016. doi: 10.1590/S0100-204X2014001000006.
http://dx.doi.org/10.1590/S0100-204X2014...
). Most characters of agronomic interest are ruled by numerous pairs of alleles, so that the study of a single character is already very complex. However, the great advantage of applying multivariate analysis is in considering the covariance between variables. As the expression of any character is influenced by numerous other characters, multivariate analysis of variance can contribute to the knowledge of the inheritance type prevailing in wheat progenies.

Table 2
Summary of multivariate analysis of variance by means of four statistical tests for agronomic traits days from emergence to silking (DES), plant height (PH), peduncle length (PL), number of spikes per plant (SPP) and grain mass per plant (GMP). Analysis for block and population effects. UDESC-IMEGEM, Lages SC, Brazil.

Contrast C1 showed a significant difference between means of parents vs. F1. The characters that most contributed to the differentiation in this comparison were as follows: DES (0.18), PL (0.84) and GMP (0.87). Thus, the heterosis phenomenon should be considered, as well as the predominance of allelic interactions of the non-additive type. Possibly, artificial hybridization resulted in the action and interaction between favorable dominant alleles expressing a greater hybrid vigor. BERTAN et al. (2009BERTAN, I. et al. Efeitos da heterose e endogamia em caracteres de importância agronômica em trigo. Revista Ceres, v.56, n.6, p.753-763, 2009. Available from: http://www.redalyc.org/articulo.oa?id=305226942008>. Accessed: Jan. 10, 2017.
http://www.redalyc.org/articulo.oa?id=30...
) studied traits in wheat and reported different heterosis levels in all characters analyzed as a function of specific effects in each cross.

Significant differences were observed between genetic constitutions P2 and P1 vs. P2_P1 (F1) (contrast C3). Combination between parents allowed the assembly of different alleles and provided the appearance of heterozygous loci in generation F1. The characters positively contributed to differentiate the treatments, except SPP (-1.86), which presented the lowest canonical weight, i.e., the lowest relative contribution to distinguish between mean vectors.

The multivariate contrasts between the parental average and the respective F1 progeny revealed the presence of significant differences, except contrast C2 (P2 and P3 vs. P2_P3 (F1)). This result suggested that a higher degree of relationship between the parents mentioned above may exist, bringing together alleles identical by ancestry in the same locus (MALÉCOT, 1966MALÉCOT, G. Probabilités et hérédité. Paris: Presses, Universitaires de France 1966. 356p.). Comparison between progenies P2_P3 F1 and P2_P3 (F2) (C8) also revealed no significant difference. The mean vectors for the set of characters evaluated for the genetic constitutions mentioned above were not significant. Thus, it can be established that most of the genome in these populations is represented by homozygous loci, which were not contrasting for the traits studied.

Another form of inbreeding that can lead to increases in the number of homozygous loci depends on the degree of relationship between the parents involved in the crossing. The higher the parentage relationship, the greater the probability that two genes present in a certain locus of this individual, are identical by ancestry, which is also known as inbreeding coefficient (MALÉCOT, 1966MALÉCOT, G. Probabilités et hérédité. Paris: Presses, Universitaires de France 1966. 356p.).

The multivariate contrasts showed that the PH and SPP characters were not changed in 75% of linear comparisons between means, independent of the genetic constitution evaluated (Table 3). Wide genetic variability was not observed in the characters mentioned in the study populations, possibly because there are gene blocks associated with expression of this character. The use of recurrent selection is one of the strategies recommended to break such threshold. This breeding method increases the frequency of favorable alleles in a population through selection and cross-linking cycles, explores genetic variability, and results in a greater probability of obtaining genetic gains (ALVES et al., 2015ALVES, A.F. et al. Genetic progress and potential of common bean families obtained by recurrent selection. Crop Breeding and Applied Biotechnology, v.15, p.218-226, 2015. Available from: http://dx.doi.org/10.1590/1984-70332015v15n4a38>. Accessed: Aug. 12, 2016. doi: 10.1590/1984-70332015v15n4a38.
http://dx.doi.org/10.1590/1984-70332015v...
).

Table 3
Multivariate test for contrasts and standardized canonical coefficients (SCC) for response variables: days from emergence to silking (DES), plant height (PH), peduncle length (PL), number of spikes per plant (SPP) and grain mass per plant (GMP). Wilk's U statistics. UDESC-IMEGEM, Lages SC, Brazil.

Only the contrasts of means C7 and C12 (between progenies F1 vs. progenies F2) revealed significant differences. In these crossings, allele segregation significantly affected the characters through self-fertilization, mainly PL (0.82) and GMP (0.53) in contrast C7, and DES (0.06), PL (0.63), and GMP (1.43) in contrast C12.

Since inheritance is a genetic information, it belongs to a particular trait and a given population, significant differences were not observed in the comparisons (67%) between progenies F1 vs. F2. Thus, predominance of additive-type genetic inheritance occurred. One of the most important characteristics of additive-type allelic interaction is that selection can be facilitated, as an individual (or group of superior individuals) produces an offspring, which is also superior when selected (CHELAIFA et al., 2013CHELAIFA, H. et al. Prevalence of gene expression additivity in genetically stable wheat allohexaploids. New Phytologist, v.197, p.730-736, 2013. Available from: http://dx.doi.org/10.1111/nph.12108>. Accessed: Aug. 20, 2016. doi: 10.1111/nph.12108.
http://dx.doi.org/10.1111/nph.12108...
).

This result demonstrated the advanced state of improvement of elite cultivars used as parents, which resulted from a long and continuous process of launching new cultivars. The latest achievements resulted in capitalization of most of the additive genetic inheritance available. When dominance is absent in the segregating population, genetic variance is equal for all gene frequencies (HALLAUER, 1975HALLAUER, A.R. Relation of gene action and type of testers in maize breeding programs. Annual Corn Sorghum Conference, v.30, p.150-159, 1975.). In Brazil, recurrent use of genotypes with desirable characteristics and high overall combining ability directly contributes to restrict the genetic basis of wheat (BERED et al., 2000BERED, F. et al. Variabilidade genética em trigo. Biotecnologia Ciência & Desenvolvimento, n.14, p.22-25, 2000. Available from: http://biotecnologia.com.br/revista/bio14/variabi.pdf>. Accessed: Sept. 01, 2016.
http://biotecnologia.com.br/revista/bio1...
). This information was confirmed by the study conducted by SOUZA & CAIERÃO (2014SOUZA, C.N.A.; CAIERÃO, E. Cultivares de trigo indicadas para cultivo no Brasil e instituições criadoras 1922 a 2014. 2.ed. Brasília: Embrapa, 2014. 200p.). These authors reported that 547 wheat cultivars were indicated for cultivation in Brazil in the period 1922-2014, and only 11 parents were more often used to generate new cultivars, with predominance of the ‘Frontana’ cultivar. According to LOPES et al. (2015LOPES, M.S. et al. Exploiting genetic diversity from landraces in wheat breading for adaptation to climate change. Journal of Experimental Botany, v.66, n.12, p.3477-3486, 2015. Available from: http://dx.doi.org/10.1093/jxb/erv122>. Accessed: Sept. 10, 2016. doi: 10.1093/jxb/erv122.
http://dx.doi.org/10.1093/jxb/erv122...
), there are two potential bottlenecks in wheat diversity. The first one is related to the recent origin of wheat (about 8000 years ago), assuming that there are relatively few crosses between tetraploid and diploid parents. Thus, only part of the diversity of T. dicoccoides and Aegilops squarosa is present in wheat. The second bottleneck is related to founding lines for local populations, where breeding programs often depend on a relatively limited number of parental lineages (elite lineages) in the development of germplasm.

On the other hand, each wheat breeding cycle requires from four to eight years until the production of genetically uniform lines, and at least three years of experimentation, to conduct comparative tests with witness varieties that are in cultivation, which corresponds to 10-12 years so that the new cultivar reaches the crops. Thus, knowledge of the predominant additive-type inheritance allows the breeder to accelerate character fixation. This may represent an increase in the production of test lineages aiming to indicate new cultivars and, above all, a greater speed in the incorporation of new resistance or quality genes in adapted cultivars.

CONCLUSION:

The multivariate analysis of variance indicated that inheritance of characters in wheat is predominantly governed by the additive-type genetic variance. Knowledge of additive-type inheritance may represent a greater speed in the incorporation of new resistance or quality genes in adapted cultivars.

ACKNOWLEDGMENTS

To the Universidade do Estado de Santa Catarina (UDESC), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Apoio à Pesquisa Científica e Tecnológica do Estado de Santa Catarina (FAPESC) for the scholarship granting and financial support fot the development of the presente research

REFERENCES:

  • 0
    CR-2016-0859.R1

Publication Dates

  • Publication in this collection
    2017

History

  • Received
    18 Sept 2016
  • Accepted
    23 Mar 2017
  • Reviewed
    10 May 2017
Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br