Optimizing <i>in vitro</i> growth of basil using LED lights

Oliveira, Rayssa Camargo de; Luz, José Magno Queiroz; Santos, Andréia Pereira dos; Oliveira, Roberta Camargos de; Blank, Arie Fitzgerald

doi:10.1590/0103-8478cr20220030

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biology • Cienc. Rural 53 (5) • 2023 • https://doi.org/10.1590/0103-8478cr20220030 copy

Optimizing in vitro growth of basil using LED lights

Otimização crescimento in vitro de manjericão usando lâmpadas LED

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

Tissue culture is an important technique for assessing the influence of light on plant growth. This study evaluated the in vitro cultivation of Ocimum basilicum cultivars under different light sources. First, an experiment was conducted to evaluate the effect of fluorescent and LED (Light Emitting Diode) light sources (yellow, blue, green, and red) on five basil cultivars (Cinnamon, Grecco a Palla, Italian Large, Limoncino, and Maria Bonita), followed by, another experiment, with two LED lights (Growlux and Blue). In vitro basil plants did not exhibit good growth under yellow lamps. The size of the roots of Cinnamon, Grecco a Palla and Limoncino cultivars under the two light conditions were similar. Moreover, the luminous condition of the Growlux lamps generated a greater quantity of leaves and fresh, and dry weight than blue LED lamps for Cinnamon, Grecco a Palla and Limoncino cultivars. Thus, it was concluded that the Growlux lamps promoted a greater amount of basil biomass.

Key words:
Ocimum basilicum; micropropagation; fluorescent; cultivars

Universidade Federal de Santa Maria Universidade Federal de Santa Maria, Centro de Ciências Rurais , 97105-900 Santa Maria RS Brazil , Tel.: +55 55 3220-8698 , Fax: +55 55 3220-8695 - Santa Maria - RS - Brazil
E-mail: cienciarural@mail.ufsm.br

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[1] E-mail: rayssacamargo@yahoo.com.br. ^*Corresponding author

Cultivars	LEA	NSH	LSH (cm)	FWH(g)	DWH(g)	SPD
Cinnamon	4.24 b	1.79 a	1.48 ab	0.12 c	0.016 b	5.20 b
Grecco a P.	8.65 a	1.81 a	1.84 ab	0.20 b	0.017 b	8.20 b
Italian L.L.	3.77 b	1.73 a	1.20 b	0.19 bc	0.018 b	13.72 a
Limoncino	5.08 b	1.76 a	2.69 a	0.25 b	0.025 b	14.80 a
Maria B.	6.12 b	2.05 a	1.29 ab	0.43 a	0.043 a	13.54 a
---------------------------------------------------------------------------------Lamps------------------------------------------------------------------------------
Yellow	5.04 bc	1.74 a	1.44 ab	0.22 ab	0.018 ab	10.32 b
Blue	9.38 a	1.70 a	2.74 a	0.26 ab	0.022 ab	13.84 a
Red	4.50 c	1.60 a	1.32 b	0.29 a	0.026 a	9.98 b
Green	6.40 ab	1.74 a	1.86 ab	0.16 b	0.016 b	10.62 b
Fluorescent	5.64 bc	2.04 a	1.7 ab	0.25 ab	0.022 ab	10.70 ab
CV	47.31	31.57	30.84	78.79	65.21	32.37

Cultivars	---------------LEA--------------		---------------------NSH-----------------------			---------------------LSH (cm)-------------------
	Blue	Growlux	Blue	Growlux		Blue		Growlux
Cinnamon	8.27 Ba	18.72 Aab	1.22 Aa	2.35 Ab		2.46 Aa		3.36 Ab
G.a Palla	11.70 Ba	23.77 Aa	1.92 Ba	5.07 Aa		2.67 Aa		2.56 Ab
Limoncino	8.20 Ba	13.90 Ab	1.52 Aa	2.12 Ab		1.79 Ba		4.88 Aa
CV	---------------23.36-------------		---------------------32.37----------------------			------------------------22.29---------------------
Cultivars	--------------LRO (cm)--------		----------FWH (g)--------		-------------DWH (g)------------		-------------SPD--------------
	Blue	Growlux	Blue	Growlux	Blue	Growlux	Blue	Growlux
Cinnamon	2.57Aa	4.87Aa	0.326 B	0.956 A	0.108 B	0.220 A	17.14 a	17.01 ab
G.a Palla	3.44Aa	4.71Aa	0.370 B	1.144 A	0.080 B	0.260 A	8.84 b	11.75 b
Limoncino	3.27Aa	3.22Aa	0.396 B	1.105 A	0.119 B	0.212 A	17.29 a	20.81 a
CV	---------------38.09-------------		-----------72.75-----------		--------------29.90----------------		--------------20.06------------